BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0019.p (251 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1697 [L] COG3935 Putative primosome component and related prot... 130 2e-30 lin2009 [L] COG3935 Putative primosome component and related pro... 122 7e-28 SA1286 [L] COG3935 Putative primosome component and related prot... 86 7e-17 SPy1482 [L] COG3935 Putative primosome component and related pro... 69 6e-12 SPy0953 [L] COG3935 Putative primosome component and related pro... 69 6e-12 >BH1697 [L] COG3935 Putative primosome component and related proteins Length = 233 Score = 130 bits (328), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 77/231 (33%), Positives = 124/231 (53%), Gaps = 33/231 (14%) Query: 17 LQNGMAVIPYALLKYYRKLNLTGSEAMLLIHLLSFRQVEGMEFPSLEELQTVTGRSIAAI 76 L+ G IP L+ +YR+L+L E + L+H+ +F + EG FP+ ++L + + Sbjct: 10 LEAGALSIPKLLMHHYRQLSLDEQELVALLHIQTFIE-EGSSFPTPDDLASRMSLASVEC 68 Query: 77 AGELQKLMKEGFISIDEDIDELRDIHYERYNFSGLYGKLGAFLAGAAQDPAQGSSKPAGT 136 + L L+K+G +++++ DE + YE+++ S L+ KL +A + + + Sbjct: 69 SKCLNGLIKKGCLALEKRSDE-EGVIYEQFSLSPLWEKLAELVAREIKQLEEKRGQ---- 123 Query: 137 VPSLHAGAPAPDGGYGRAFTSAAGKDAEENRSLFTIFEKEFGRPLSPMECETISGWVDQD 196 EE +L+ +FE+EF RPLSP+E ET+S W+DQD Sbjct: 124 ---------------------------EEEMTLYRLFEREFSRPLSPIEGETLSMWIDQD 156 Query: 197 NYPEELILLALKESVFAGKIHFRYIDRILLEWARNRVKNAQDVKTYTQKFR 247 + ELI AL+E+V +GK++FRYIDRIL EW +N V K Y +KFR Sbjct: 157 QHTPELIKAALREAVISGKLNFRYIDRILFEWKKNGVTTLDQAKKYGEKFR 207 >lin2009 [L] COG3935 Putative primosome component and related proteins Length = 239 Score = 122 bits (305), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 84/251 (33%), Positives = 119/251 (47%), Gaps = 40/251 (15%) Query: 1 MDGKAWNAWGEGVSFGLQNGMAVIPYALLKYYRKLNLTGSEAMLLIHLLSFRQVEGMEFP 60 MD K W + G +P L+K Y + L +E +LL+ + SF E FP Sbjct: 1 MDHKMLEKW-------MAEGQVTLPQVLVKNYAAIGLNENELVLLLQIQSF-AAEAEFFP 52 Query: 61 SLEELQTVTGRSIAAIAGELQKLMKEGFISIDEDIDELRDIHYERYNFSGLYGKLGAFLA 120 S+E L T + + L+K+ ++I++ D I E+YN L+GKL AF Sbjct: 53 SMEMLTDRTTLRLEDTIKTMDSLLKKSVVAIEQSKDNSLMIS-EQYNLEPLWGKLVAFYE 111 Query: 121 GAAQDPAQGSSKPAGTVPSLHAGAPAPDGGYGRAFTSAAGKDAEENRSLFTIFEKEFGRP 180 D Q + E+ +L+++FE EFGRP Sbjct: 112 NIEADNRQDL-------------------------------EVEQQTNLYSLFEAEFGRP 140 Query: 181 LSPMECETISGWVDQDNYPEELILLALKESVFAGKIHFRYIDRILLEWARNRVKNAQDVK 240 LSPME E +S WVDQD +LI ALKE+V + K++FRYIDRILL W++ VK D K Sbjct: 141 LSPMEAEMLSAWVDQDRTSPDLIKEALKEAVISQKLNFRYIDRILLNWSKQGVKTIDDAK 200 Query: 241 TYTQKFRGGGR 251 ++F GR Sbjct: 201 RVAEEFHQNGR 211 >SA1286 [L] COG3935 Putative primosome component and related proteins Length = 228 Score = 85.5 bits (210), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 64/235 (27%), Positives = 102/235 (43%), Gaps = 39/235 (16%) Query: 15 FGLQNGMAVIPYALLKYYRKLNLTGSEAMLLIHLLSFRQVEGMEFPSLEELQ---TVTGR 71 + L+ VI LL +Y L L + ++L+ L+ + + PS+E LQ T+ R Sbjct: 4 YQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETSNKQ-PSIELLQKGSTMQPR 62 Query: 72 SIAAIAGELQKLMKEGFISIDEDIDELRDIHYERYNFSGLYGKLGAFLAGAAQDPAQGSS 131 I + +Q L++ + + DE E N + KL L + + + +S Sbjct: 63 DITMV---IQNLIQRELLELQVQKDE-EGRFTEYMNLDPFFEKLSHILKQQSMETKEQNS 118 Query: 132 KPAGTVPSLHAGAPAPDGGYGRAFTSAAGKDAEENRSLFTIFEKEFGRPLSPMECETISG 191 K E+ + LF + E F RPLSP E ET++ Sbjct: 119 K-------------------------------EKFKQLFRVLEDTFARPLSPYEIETLNQ 147 Query: 192 WVDQDNYPEELILLALKESVFAGKIHFRYIDRILLEWARNRVKNAQDVKTYTQKF 246 W+D D + +I AL E+ K+ F+Y+DRILL W +N VK D + +KF Sbjct: 148 WIDVDKHDSAIIQAALDEANSLNKLSFKYMDRILLNWKKNNVKTIDDSRKIREKF 202 >SPy1482 [L] COG3935 Putative primosome component and related proteins Length = 128 Score = 69.3 bits (168), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 31/87 (35%), Positives = 53/87 (60%) Query: 165 ENRSLFTIFEKEFGRPLSPMECETISGWVDQDNYPEELILLALKESVFAGKIHFRYIDRI 224 + + LF F+ FGR +SP E E I W+ +DN P E++ LAL+E+V KI ++YI++I Sbjct: 2 DEKKLFENFQLTFGRMISPFEIEDIQKWIHEDNMPIEVVNLALREAVENNKISWKYINKI 61 Query: 225 LLEWARNRVKNAQDVKTYTQKFRGGGR 251 L++W ++ + V+ Q+F + Sbjct: 62 LVDWYKSGDTTVEKVRDRLQRFDDSKK 88 >SPy0953 [L] COG3935 Putative primosome component and related proteins Length = 128 Score = 69.3 bits (168), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 31/87 (35%), Positives = 53/87 (60%) Query: 165 ENRSLFTIFEKEFGRPLSPMECETISGWVDQDNYPEELILLALKESVFAGKIHFRYIDRI 224 + + LF F+ FGR +SP E E I W+ +DN P E++ LAL+E+V KI ++YI++I Sbjct: 2 DEKKLFENFQLTFGRMISPFEIEDIQKWIHEDNMPIEVVNLALREAVENNKISWKYINKI 61 Query: 225 LLEWARNRVKNAQDVKTYTQKFRGGGR 251 L++W ++ + V+ Q+F + Sbjct: 62 LVDWYKSGDTTVEKVRDRLQRFDDSKK 88 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.137 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,269,943 Number of extensions: 931404 Number of successful extensions: 2926 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 2926 Number of HSP's successfully gapped: 9 Length of query: 251 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 147 Effective length of database: 26,383,695 Effective search space: 3878403165 Effective search space used: 3878403165 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)