BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0018.p (288 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yqjO [E] COG0345 Pyrroline-5-carboxylate reductase 198 7e-51 BS_proH [E] COG0345 Pyrroline-5-carboxylate reductase 191 1e-48 BH1503 [E] COG0345 Pyrroline-5-carboxylate reductase 181 8e-46 lin0414 [E] COG0345 Pyrroline-5-carboxylate reductase 152 7e-37 AGc4015 [E] COG0345 Pyrroline-5-carboxylate reductase 129 5e-30 >BS_yqjO [E] COG0345 Pyrroline-5-carboxylate reductase Length = 346 Score = 198 bits (504), Expect = 7e-51, Method: Compositional matrix adjust. Identities = 15/279 (5%), Positives = 49/279 (17%), Gaps = 9/279 (3%) Query: 13 QIVFYGAGSMAEAIVRGMIARNVVESGNIVMLNXXXXXXXXXXXXXYGVLGSNDPEQKNE 72 + + G + S + Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVP---PRP 59 Query: 73 YLRTAPVIVLAMKPKDAAEALRSLGPLLSPDQLVISMIAGMTIRTMQGLLGKSQPVVRTM 132 + + + + + G+ + + ++ Sbjct: 60 ETQGLTETYVGNWLAKHGSREKLIIASKV-SGPSRNNDKGIRPDQALDRKNIREALHDSL 118 Query: 133 PNTSSSIGLGATGIAYSKEVDEQSRRTALNIFEAVGLTSVIDEERMETLTGISGSGPAYI 192 + + + + A ++ + + + + Sbjct: 119 KRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGV 178 Query: 193 YYMMEAMIAAGIRGGLPAEQSRELTVQTVLGAARMVQQTGEEPAALRKKVTSPNGSTQAA 252 + + + R +T+Q + G + + V S Sbjct: 179 SNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGF 238 Query: 253 LEVLEKGDFFETVISAVNRCAERSREM-----GLALEKE 286 + K A N R A+ Sbjct: 239 GTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAY 277 >BS_proH [E] COG0345 Pyrroline-5-carboxylate reductase Length = 346 Score = 191 bits (484), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 16/253 (6%), Positives = 38/253 (15%), Gaps = 4/253 (1%) Query: 7 IPLLNHQ-IVFYGAGSMAEAIVRGMIARNVVESGNIVMLNXXXXXXXXXXXXXYGVLGSN 65 + + +EA + V + N++ + Sbjct: 11 LEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVG 70 Query: 66 DPEQKNEYLRTAPVIVLAMKPKDAAEALRSLGPLLSPDQLVISMIAGMTIRTMQGLLGKS 125 + +++A K + A Sbjct: 71 -NWLA--KHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLD 127 Query: 126 QPVVRTMPNTSSSIGLGATGIAYSKEVDEQSRRTALNIFEAVGLTSVIDEERMETLTGIS 185 V ++ G S ET G+ Sbjct: 128 LYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVM 187 Query: 186 GSGPAYIYYMMEAMIAAGIRGGLPAEQSRELTVQTVLGAARMVQQTGEEPAALRKKVTSP 245 + + ++ L + + Sbjct: 188 RYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLN 247 Query: 246 NGSTQAALEVLEK 258 A L Sbjct: 248 GAKPAGARNTLFS 260 >BH1503 [E] COG0345 Pyrroline-5-carboxylate reductase Length = 346 Score = 181 bits (460), Expect = 8e-46, Method: Compositional matrix adjust. Identities = 17/272 (6%), Positives = 49/272 (18%), Gaps = 3/272 (1%) Query: 9 LLNHQIVFYGAGSMAEAIVRGMIARNVVESGNIVMLNXXXXXXXXXXXXXYGVLGSNDPE 68 + S +N + + + Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRP-- 59 Query: 69 QKNEYLRTAPVIVLAMKPKDAAEALRSLGPLLSPDQLVISMIAGMTIRTMQGLLGKSQPV 128 + + L V K + + + + + + Sbjct: 60 -ETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSL 118 Query: 129 VRTMPNTSSSIGLGATGIAYSKEVDEQSRRTALNIFEAVGLTSVIDEERMETLTGISGSG 188 R + + + T ++ T E Sbjct: 119 KRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGV 178 Query: 189 PAYIYYMMEAMIAAGIRGGLPAEQSRELTVQTVLGAARMVQQTGEEPAALRKKVTSPNGS 248 + + + + LP + + + + + + + S G Sbjct: 179 SNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGF 238 Query: 249 TQAALEVLEKGDFFETVISAVNRCAERSREMG 280 + L + +R S E Sbjct: 239 GTLTGKYLNGAKPAGARNTLFSRFTRYSGEQT 270 >lin0414 [E] COG0345 Pyrroline-5-carboxylate reductase Length = 346 Score = 152 bits (383), Expect = 7e-37, Method: Compositional matrix adjust. Identities = 8/268 (2%), Positives = 35/268 (13%), Gaps = 3/268 (1%) Query: 12 HQIVFYGAGSMAEAIVRGMIARNVVESGNIVMLNXXXXXXXXXXXXXYGVLGSNDPEQKN 71 + + G + S + P + Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 72 EYLRTAPVIVLAMKPKDAAEALRSLGPLLSPDQLVISMIAGMTIRTMQGLLGKSQPVVRT 131 + + + + + + + + Sbjct: 62 QG---LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSL 118 Query: 132 MPNTSSSIGLGATGIAYSKEVDEQSRRTALNIFEAVGLTSVIDEERMETLTGISGSGPAY 191 + + L + + E Sbjct: 119 KRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGV 178 Query: 192 IYYMMEAMIAAGIRGGLPAEQSRELTVQTVLGAARMVQQTGEEPAALRKKVTSPNGSTQA 251 ++ R + E + Sbjct: 179 SNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGF 238 Query: 252 ALEVLEKGDFFETVISAVNRCAERSREM 279 + + + + + +R Sbjct: 239 GTLTGKYLNGAKPAGARNTLFSRFTRYS 266 >AGc4015 [E] COG0345 Pyrroline-5-carboxylate reductase Length = 346 Score = 129 bits (324), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 14/267 (5%), Positives = 42/267 (15%), Gaps = 13/267 (4%) Query: 14 IVFYGAGSMAEAIVRGMIARNVVESGNIVMLNXXXXXXXXXXXXXYGVLGSNDPEQKNEY 73 + G + + + Q Sbjct: 11 LEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPV----PPRPETQGLTE 66 Query: 74 LRTAPVIVLAMKPKDAAEALRSLGPLLSPDQLVISMIAGMTIRTMQGLLGKSQPVVRTMP 133 + ++ + + I + + ++ L Sbjct: 67 TY-VGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALH-DSLKRLQTD 124 Query: 134 NTSSSIGLGATGIAYSKEVDEQSRRTALN-IFEAVGLTSVID-EERMETLTGISGSGPAY 191 + T + + +R + I S Sbjct: 125 YLDLYQVHWPQR-PTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETA 183 Query: 192 IYYMMEAMIAAGIRGGLPAEQSRELTVQTVLGAARMVQQTGEEPAALRKKVTSPNGSTQA 251 M +A ++ + + + E L G+ Sbjct: 184 FGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTG 243 Query: 252 ALEVLEKGD----FFETVISAVNRCAE 274 K + + + Sbjct: 244 KYLNGAKPAGARNTLFSRFTRYSGEQT 270 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.131 0.354 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,030,588 Number of extensions: 633012 Number of successful extensions: 1561 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 1543 Number of HSP's successfully gapped: 16 Length of query: 288 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 183 Effective length of database: 26,268,361 Effective search space: 4807110063 Effective search space used: 4807110063 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)