BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0017.p (265 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sll0373 [E] COG0014 Gamma-glutamyl phosphate reductase 214 1e-55 all2166 [E] COG0014 Gamma-glutamyl phosphate reductase 202 5e-52 alr3995 [E] COG0014 Gamma-glutamyl phosphate reductase 127 3e-29 sll0461 [E] COG0014 Gamma-glutamyl phosphate reductase 89 8e-18 >sll0373 [E] COG0014 Gamma-glutamyl phosphate reductase Length = 433 Score = 214 bits (545), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 114/261 (43%), Positives = 161/261 (61%), Gaps = 3/261 (1%) Query: 3 SNRKIIEVLHRALDETAIPSQALQLIEDPNRSSVDEMLKLNGLLDVIIPRGGSALIQNVV 62 S + + EV+ AL +T + +A+ L+ R + E+L LN +D+IIPRG + +Q + Sbjct: 159 SCQVLTEVIQTALAKTVVSPEAINLLT--TREEIRELLGLNQYVDLIIPRGSNEFVQYIQ 216 Query: 63 LNATVPVIETGAGICHTYLDAGADPEMALSISLNAKAQRPSVCNSMETLLVHRDYAAEHL 122 N +PV+ GICH YLDA AD A+ I+++AK Q P+ CN++ETLLVH+ AAE L Sbjct: 217 QNTQIPVLGHADGICHLYLDAQADLSKAIPITVDAKTQYPAACNAIETLLVHQAIAAEFL 276 Query: 123 LPLAEAFRKIPVELRGCPETTALIPWALPAAREDFATEYNDYILNIKIVGGLDEALQHIA 182 PLA+A + V LRG T LI PA D+ TEY+D IL+IKIV L+ A+ HI Sbjct: 277 PPLAQALGEKGVSLRGDSGTQKLIDCE-PATEADWCTEYSDLILSIKIVDSLEAAIDHIN 335 Query: 183 EFSTKHSECIVTRDVGNAERFLQEVDAAAVYHNASTRFTDXXXXXXXXXXXXSTQKLHAR 242 ++ +KH++ I++ D+ AE+F Q VD+A VYHN STRF D STQK+ R Sbjct: 336 QYGSKHTDGIISEDLTAAEQFCQRVDSAGVYHNCSTRFADGFRYGFGAEVGISTQKMPPR 395 Query: 243 GPMGLPALTSSKYKIHGSGQI 263 GP+GL L + KY++ G G I Sbjct: 396 GPVGLEGLVTYKYQLWGHGHI 416 >all2166 [E] COG0014 Gamma-glutamyl phosphate reductase Length = 252 Score = 202 bits (514), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 0/263 (0%), Positives = 0/263 (0%), Gaps = 2/263 (0%) Query: 1 LSSNRKIIEVLHRALDETAIPSQALQLIEDPNRSSVDEMLKLNGLLDVIIPRGGSALIQN 60 I + + Q+ +++ +D + + + Sbjct: 161 GVHAYYDILSEAGCEVDIWRTTYYHQMP--SHQAIIDWVTATGLRPWLQDLTESEQQLFL 218 Query: 61 VVLNATVPVIETGAGICHTYLDAGADPEMALSISLNAKAQRPSVCNSMETLLVHRDYAAE 120 Sbjct: 219 278 Query: 121 HLLPLAEAFRKIPVELRGCPETTALIPWALPAAREDFATEYNDYILNIKIVGGLDEALQH 180 Sbjct: 279 338 Query: 181 IAEFSTKHSECIVTRDVGNAERFLQEVDAAAVYHNASTRFTDXXXXXXXXXXXXSTQKLH 240 Sbjct: 339 398 Query: 241 ARGPMGLPALTSSKYKIHGSGQI 263 Sbjct: 399 421 >alr3995 [E] COG0014 Gamma-glutamyl phosphate reductase Length = 252 Score = 127 bits (318), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 0/261 (0%), Positives = 0/261 (0%) Query: 3 SNRKIIEVLHRALDETAIPSQALQLIEDPNRSSVDEMLKLNGLLDVIIPRGGSALIQNVV 62 ++L A E I +++ +D + + + Sbjct: 161 GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKR 220 Query: 63 LNATVPVIETGAGICHTYLDAGADPEMALSISLNAKAQRPSVCNSMETLLVHRDYAAEHL 122 Sbjct: 221 280 Query: 123 LPLAEAFRKIPVELRGCPETTALIPWALPAAREDFATEYNDYILNIKIVGGLDEALQHIA 182 Sbjct: 281 340 Query: 183 EFSTKHSECIVTRDVGNAERFLQEVDAAAVYHNASTRFTDXXXXXXXXXXXXSTQKLHAR 242 Sbjct: 341 400 Query: 243 GPMGLPALTSSKYKIHGSGQI 263 Sbjct: 401 421 >sll0461 [E] COG0014 Gamma-glutamyl phosphate reductase Length = 420 Score = 89.0 bits (219), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 64/258 (24%), Positives = 109/258 (42%), Gaps = 1/258 (0%) Query: 3 SNRKIIEVLHRALDETAIPSQALQLIEDPNRSSVDEMLKLNGLLDVIIPRGGSALIQNVV 62 S I E+L L + +P ++ I +V +++ L+++IP G + ++ + Sbjct: 159 STAAICEILREGLLDADLPVDSVSHIPSETSPNVQDLVGNASQLNLVIPYGRPSFVEQIS 218 Query: 63 LNATVPVIETGAGICHTYLDAGADPEMALSISLNAKAQRPSVCNSMETLLVHRDYAAEHL 122 T PV++ G C+ Y + D EM + +++ P N++E +LV L Sbjct: 219 QQCTPPVLKAAMGNCYLYWSSKGDLEMVRQMIIDSHVGHPDPVNAIEKVLVSPGQNPAPL 278 Query: 123 LPLAEAFRKIPVELRGCPETTALIPWALPAARE-DFATEYNDYILNIKIVGGLDEALQHI 181 + L + +LRG E P L A+E ++ Y D + + L A+ I Sbjct: 279 VRLLNNLQAKGFKLRGDAELCEQFPDHLTLAKENEWGKAYLDRTVAFRTTQNLKTAIAWI 338 Query: 182 AEFSTKHSECIVTRDVGNAERFLQEVDAAAVYHNASTRFTDXXXXXXXXXXXXSTQKLHA 241 S+ H +CI T + +F VD+A VY N F S+QK Sbjct: 339 NSHSSGHGDCIATDSYQESRQFSMGVDSALVYVNIPPYFCRNPRHGESLFLGVSSQKGQR 398 Query: 242 RGPMGLPALTSSKYKIHG 259 RG +GL A + K + G Sbjct: 399 RGLIGLEAFMTPKQIVQG 416 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.133 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,567,603 Number of extensions: 696529 Number of successful extensions: 2520 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 2518 Number of HSP's successfully gapped: 4 Length of query: 265 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 161 Effective length of database: 26,383,695 Effective search space: 4247774895 Effective search space used: 4247774895 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)