BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0016.p (458 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_proB [E] COG0263 Glutamate 5-kinase 278 2e-74 BS_proJ [E] COG0263 Glutamate 5-kinase 255 1e-67 SMc03776 [E] COG0263 Glutamate 5-kinase 228 2e-59 BS_proB [E] COG0263 Glutamate 5-kinase 220 4e-57 mll4011 [E] COG0263 Glutamate 5-kinase 215 1e-55 >BS_proB [E] COG0263 Glutamate 5-kinase Length = 367 Score = 278 bits (710), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 23/313 (7%), Positives = 54/313 (17%), Gaps = 1/313 (0%) Query: 1 VVKIGSSSLTGPEGGLNRKAVAFFAAEIAGLQKDGCEVLLVTSGAVAAGFRAIGYPARPK 60 +V +S+ +A +++ + A R Sbjct: 7 LVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHRIVIVTSGAIAAGREHLGYPELP 66 Query: 61 LLHEKQAAAAVGQVMLMQAYQEAFAEYGIPTAQILLTRTDFCSRRAMNNAVMTVEELLRQ 120 + A + E +L R L Sbjct: 67 ATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQMLLTRADMEDRERFLNARDTLRALLD 126 Query: 121 GAVPIFNENDTVSVDELKFGDNDTLSALVANLLKASHLLVLTDMDGLYSGDPRKHPGATR 180 + + ++ + +A +L + L+L + Sbjct: 127 NNIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQKGLYTADPRSNPQAE 186 Query: 181 YERVEEITPEIYAIXXXXXXXXXXXXMRSKIDAAKIATRGGVPVFVGRATEPGDLQLAAA 240 + + S A + A + Sbjct: 187 LIKDVYGIDDALRAIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIAAGSKPGVIGDVM 246 Query: 241 GTGKGTYF-DTTLSSLPVKKQWLGFMSTPLGSLYVDEGAVEALLHGGHSLLPVGVKRIEG 299 + L +K+W+ + L G L Sbjct: 247 EGISVGTLFHAQATPLENRKRWIFGAPPAGEITVDEGATAAILERGSSLLPKGIKSVTGN 306 Query: 300 SFHSGDVVEVLGP 312 + Sbjct: 307 FSRGEVIRICNLE 319 >BS_proJ [E] COG0263 Glutamate 5-kinase Length = 252 Score = 255 bits (651), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 0/312 (0%), Positives = 0/312 (0%), Gaps = 3/312 (0%) Query: 1 VVKIGSSSLTGPEGGLNRKAVAFFAAEIAGLQKDGCEVLLVTSGAVAAGFRAIGYPARPK 60 S + A++ + +L A + Sbjct: 11 HFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPAMIAE 70 Query: 61 LLHEKQAAAAVGQVMLMQAYQEAFAEYGIPTAQILLTRTDFCSRRAMNNAVMTVEELLRQ 120 V + +A + L ++ ++ Sbjct: 71 ARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQM 130 Query: 121 GAVPIFNENDTVSVDELKFGDNDTLSALVANLLKASHLLVLTDMDGLYSGDPRKHPGATR 180 + + + + D +A + +L + H + Sbjct: 131 PDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPSH 190 Query: 181 YERVEEITPEIYAIXXXXXXXXXXXXMRSKIDAAKIATRGGVPVFVGRATEPGDLQLAAA 240 ++ +T + P +A Sbjct: 191 QAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLAFPRLFIVARR 250 Query: 241 GTGKGTYFDTTLSSLPV--KKQWLGFMSTPLGSLYVDEGAVEALLHGGHSLLPVGVKRIE 298 Sbjct: 251 - 309 Query: 299 GSFHSGDVVEVL 310 Sbjct: 310 321 >SMc03776 [E] COG0263 Glutamate 5-kinase Length = 252 Score = 228 bits (580), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 0/316 (0%), Positives = 0/316 (0%), Gaps = 4/316 (1%) Query: 1 VVKIGSSSLTGPEGGLNRKAVAFFAAEIAGLQKDGCEVLLVTSGAVAAGFRAIGYPARPK 60 + L N + VA + A+ AR Sbjct: 15 ERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPAMIAEARSA 74 Query: 61 LLHEKQAAAAVGQVMLMQAYQEAFAEYGIPTAQILLTRTDFCSRRAMNNAVMTVEELLRQ 120 L + A + +QA FA + V+ V+ Sbjct: 75 LPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQMPDNW 134 Query: 121 GAVPIFNENDTVSVDELKFGDNDTLSALVANLLKASHLLVLTDMDGLYSGDPRKHPGATR 180 + + L+ + A S D+ A Sbjct: 135 LEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAII 194 Query: 181 YERVEEITPEIYAIXXXXXXXXXXXXMRSKIDAAKIATRGGVPVFVGRATEPGDLQLAAA 240 ++ G + + Sbjct: 195 DWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLA--FPRLFIVARRME 252 Query: 241 GTG-KGTYFDTTLSSLPVKKQWLGFMSTPLGSLYVDEGAVEALLHGGHSLLPVGVKRIEG 299 Sbjct: 253 - 311 Query: 300 SFHSGDVVEVLGPDAR 315 Sbjct: 312 327 Score = 220 bits (560), Expect = 4e-57, Method: Compositional matrix adjust. Identities = 126/315 (40%), Positives = 173/315 (54%), Gaps = 1/315 (0%) Query: 1 VVKIGSSSLTGPEGGLNRKAVAFFAAEIAGLQKDGCEVLLVTSGAVAAGFRAIGYPARPK 60 VVK+G+S LTG LNR + + A L G +++VTSGA+AAG +GYP P Sbjct: 8 VVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHRIVIVTSGAIAAGREHLGYPELPA 67 Query: 61 LLHEKQAAAAVGQVMLMQAYQEAFAEYGIPTAQILLTRTDFCSRRAMNNAVMTVEELLRQ 120 + KQ AAVGQ L+Q +++ F+ YGI Q+LLTR D R NA T+ LL Sbjct: 68 TIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQMLLTRADMEDRERFLNARDTLRALLDN 127 Query: 121 GAVPIFNENDTVSVDELKFGDNDTLSALVANLLKASHLLVLTDMDGLYSGDPRKHPGATR 180 VP+ NEND V+ E+K GDND LSAL A L A LL+LTD GLY+ DPR +P A Sbjct: 128 NIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQKGLYTADPRSNPQAEL 187 Query: 181 YERVEEITPEIYAIXXXXXXXXXXXXMRSKIDAAKIATRGGVPVFVGRATEPGDLQLAAA 240 + V I + AI M +K+ AA +A R G+ + ++PG + Sbjct: 188 IKDVYGIDDALRAIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIAAGSKPGVIGDVME 247 Query: 241 GTGKGTYFDTTLSSLPVKKQWLGFMSTPLGSLYVDEGAVEALLHGGHSLLPVGVKRIEGS 300 G GT F + L +K+W+ F + P G + VDEGA A+L G SLLP G+K + G+ Sbjct: 248 GISVGTLFHAQATPLENRKRWI-FGAPPAGEITVDEGATAAILERGSSLLPKGIKSVTGN 306 Query: 301 FHSGDVVEVLGPDAR 315 F G+V+ + + R Sbjct: 307 FSRGEVIRICNLEGR 321 >mll4011 [E] COG0263 Glutamate 5-kinase Length = 252 Score = 215 bits (548), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 0/314 (0%), Positives = 0/314 (0%), Gaps = 2/314 (0%) Query: 2 VKIGSSSLTGPEGGLNRKAVAFFAAEIAGLQKDGCEVLLVTSGAVAAGFRAIGYPARPKL 61 S + A++ + +L A + Sbjct: 12 FSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPAMIAEA 71 Query: 62 LHEKQAAAAVGQVMLMQAYQEAFAEYGIPTAQILLTRTDFCSRRAMNNAVMTVEELLRQG 121 V + +A + L ++ ++ Sbjct: 72 RSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVLAVQMP 131 Query: 122 AVPIFNENDTVSVDELKFGDNDTLSALVANLLKASHLLVLTDMDGLYSGDPRKHPGATRY 181 + + + + D +A + +L + H + Sbjct: 132 DNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQMPSHQ 191 Query: 182 ERVEEITPEIYAIXXXXXXXXXXXXMRSKIDAAKIATRGGVPVFVGRATEPGDLQLAAAG 241 ++ +T + P +A Sbjct: 192 AIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLAFPRLFIVARRM 251 Query: 242 TGKGTYFDTTLSSLPVKKQWLGFMSTPLGSLYVDEGAVEALLHGGHSLLPVGVKRIEGSF 301 Sbjct: 252 E-- 309 Query: 302 HSGDVVEVLGPDAR 315 Sbjct: 310 323 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.133 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,178,700 Number of extensions: 1157365 Number of successful extensions: 2099 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 2091 Number of HSP's successfully gapped: 10 Length of query: 458 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 348 Effective length of database: 25,691,691 Effective search space: 8940708468 Effective search space used: 8940708468 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)