BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0011.p (474 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0111 [E] COG0065 3-isopropylmalate dehydratase large subunit 615 e-176 STM0329 [E] COG0065 3-isopropylmalate dehydratase large subunit 472 e-133 PAB0891 [E] COG0065 3-isopropylmalate dehydratase large subunit 244 2e-64 MJ0499 [E] COG0065 3-isopropylmalate dehydratase large subunit 242 7e-64 MJ1003 [E] COG0065 3-isopropylmalate dehydratase large subunit 231 2e-60 >STM0111 [E] COG0065 3-isopropylmalate dehydratase large subunit Length = 346 Score = 615 bits (1585), Expect = e-176, Method: Compositional matrix adjust. Identities = 0/461 (0%), Positives = 0/461 (0%), Gaps = 1/461 (0%) Query: 4 KTMFEKIWDNHVIHQEEGKPSIIYIDLHLVHEVTSPQAFEGLRLSGRKVRRPGLTFATMD 63 H +G+ L P Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 64 HNVPTKDR-YNITDPISKQQIDTLSQNCRDFGVKLFDLNDIDQGVVHVMGPEIGLTHPGK 122 T + ++ I + D ++ + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQ 122 Query: 123 TIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLQQSKAKTMEVRFTGKRNPGVTAKD 182 T +G S + A L A R R GV+ + Sbjct: 123 TDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNET 182 Query: 183 MILGVIAKYGTDFATGYVIEYTGEAIRELSMEERMTVCNMSIEGGARAGLIAPDETTFNY 242 + + D I L+ + + +S G + Sbjct: 183 AFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLT 242 Query: 243 LRGRQYVPQGAAYEAAVETWKELVSDEGAQYDTVVEFDVETLIPQVTWGTSPGMGTNITS 302 + A + ++ + + Sbjct: 243 GKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFV 302 Query: 303 TVPNPADFTTENERKAAEKALEYMDLVPGTPISEIGIDYVFIGSCTNGRIEDLRAAAEVA 362 Sbjct: 303 362 Query: 363 KGHKVSDKVTAIVVPGSGRVKMQAEKEGLDKVFTEAGFEWREAGCSMCLAMNPDVLQPGQ 422 Sbjct: 363 422 Query: 423 RCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAIKGHFTDVR 463 Sbjct: 423 463 >STM0329 [E] COG0065 3-isopropylmalate dehydratase large subunit Length = 346 Score = 472 bits (1214), Expect = e-133, Method: Compositional matrix adjust. Identities = 0/466 (0%), Positives = 0/466 (0%), Gaps = 3/466 (0%) Query: 1 MGNKTMFEKIWDNHVIHQEEGKPSII-YIDLHLVHEVTSPQAFEGLRLSGRKVRRPGLTF 59 M + + + + L P Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 60 ATMDHNVPTKDRYN--ITDPISKQQIDTLSQNCRDFGVKLFDLNDIDQGVVHVMGPEIGL 117 + + + + D + + + + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 118 THPGKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLQQSKAKTMEVRFTGKRNPG 177 + T V L + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 178 VTAKDMILGVIAKYGTDFATGYVIEYTGEAIRELSMEERMTVCNMSIEGGARAGLIAPDE 237 TA ++ + D I+ + V + Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGT 240 Query: 238 TTFNYLRGRQYVPQGAAYEAAVETWKELVSDEGAQYDTVVEFDVETLIPQVTWGTSPGMG 297 T YL G + + + + + + Q+ Sbjct: 241 LTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQP 300 Query: 298 TNITSTVPNPADFTTENERKAAEKALEYMDLVPGTPISEIGIDYVFIGSCTNGRIEDLRA 357 Sbjct: 301 360 Query: 358 AAEVAKGHKVSDKVTAIVVPGSGRVKMQAEKEGLDKVFTEAGFEWREAGCSMCLAMNPDV 417 Sbjct: 361 420 Query: 418 LQPGQRCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAIKGHFTDVR 463 Sbjct: 421 466 >PAB0891 [E] COG0065 3-isopropylmalate dehydratase large subunit Length = 346 Score = 244 bits (623), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 0/460 (0%), Positives = 0/460 (0%), Gaps = 53/460 (11%) Query: 5 TMFEKIWDNHVIHQEEGKPSIIY---IDLHLVHEVTSPQAFEGLR-LSGRKVRRPGLTFA 60 G + + L ++ P Sbjct: 4 HRIPHSSLEVS-TLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 61 TMDHNVPTKDRYNITDPISKQQIDTLSQNCRDFGVKLFDLNDIDQGVVHVMGPEIGLTHP 120 + + L + G + + + I Sbjct: 63 GLTETYVGNWLA------KHGSREKLIIASKVSGPSRN-NDKGIRPDQALDRKNIREALH 115 Query: 121 GKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLQQSKAKTMEVRFTGKRNPGVTA 180 + + ++ + S T++ +R + Sbjct: 116 DSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRY 175 Query: 181 KDMILGVIAKYGTDFATGYVIEYTGEAIRELSMEERMTVCNMSIEGGARAGLIAPDETTF 240 + + + + + ++ + + Sbjct: 176 IGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSC 235 Query: 241 NYLRGRQYVPQGAAYEAAVETWKELVSDEGAQYDTVVEFDVETLIPQVTWGTSPGMGTNI 300 + Q Sbjct: 236 LGFGTL-----------TGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYV------ 278 Query: 301 TSTVPNPADFTTENERKAAEKALEYMDLVPGTPISEIGIDYVFIGSCTNGRIEDLRAAAE 360 + + + Sbjct: 279 DIARRH---------GLDPA--------------QMALAFVRRQPFVASTLLGATTMDQL 315 Query: 361 VAKGHKVSDKVTAIVVPGSGRVKMQAEKEGLDKVFTEAGFEWREAGCSMCLAMNPDVLQP 420 Sbjct: 316 375 Query: 421 GQRCASTSNRNFEGRQGR-GGRTHLVSPAMAAAAAIKGHF 459 Sbjct: 376 415 >MJ0499 [E] COG0065 3-isopropylmalate dehydratase large subunit Length = 346 Score = 242 bits (618), Expect = 7e-64, Method: Compositional matrix adjust. Identities = 0/467 (0%), Positives = 0/467 (0%), Gaps = 52/467 (11%) Query: 5 TMFEKIWDNHVIHQEEGKPSIIY--IDLHLVHEVTSPQAFEGLRLSG-RKVRRPGLTFAT 61 + L P Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 62 MDHNVPTKDRYNITDPISKQQIDTLSQN-CRDFGVKLFDLNDIDQGVVHVMGPEIGLTHP 120 + + ++K + G ND + + Sbjct: 64 LTETYVG-------NWLAKHGSREKLIIASKVSGPSRN--NDKGIRPDQALDRKNIREAL 114 Query: 121 GKTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLQQSKAKTMEVRFTGKRNPGVTA 180 ++ + T + G Sbjct: 115 HDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIR 174 Query: 181 KDMILGVIAKYGTDFATGYVIEYTGEAIRELSMEERMTVCNMSIEGGARAGLIAPDETTF 240 + A + + + + Sbjct: 175 YIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYS 234 Query: 241 NYLRGRQYVPQGAAYEAAVETWKELVSDEGAQYDTVVEFDVETLIPQVTWGTSPGMGTNI 300 + + T + Sbjct: 235 ------CLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAV-------------- 274 Query: 301 TSTVPNPADFTTENERKAAEKALEYMDLVPGTPISEIGIDYVFIGSCTNGRIEDLRAAAE 360 + V + A+ + + + T + Sbjct: 275 AAYVDIARR----HGLDPAQ--------MALAF-----VRRQPFVASTLLGATTMDQLKT 317 Query: 361 VAKGH-KVSDKVTAIVVPGSGRVKMQAEKEGLDKVFTEAGFEWREAGCSMCLAMNPDVLQ 419 + Sbjct: 318 NIESLH 377 Query: 420 PGQRCASTSNRNFEGRQGR-GGRTHLVSPAMAAAAAIKGHFTDVRDW 465 Sbjct: 378 424 >MJ1003 [E] COG0065 3-isopropylmalate dehydratase large subunit Length = 346 Score = 231 bits (589), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 0/465 (0%), Positives = 0/465 (0%), Gaps = 51/465 (10%) Query: 5 TMFEKIWDNHVIHQEEGKPSI-IYIDLHLVHEVTSPQAFEGLRLSGRKVRRPGLTFATMD 63 + + + A + Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 64 HNVPTKDRYNITDPISKQQIDTLS-QNCRDFGVKLFDLNDIDQGVVHVMGPEIGLTHPGK 122 + + S + + + Sbjct: 62 QGLTE-------TYVGNWLAKHGSREKLIIASKVSGPSRN--NDKGIRPDQA-LDRKNIR 111 Query: 123 TIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCLQQSKAKTMEVRFTGKRNPGVTAKD 182 + A + + T + Sbjct: 112 EALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLD-ALAEYQRA 170 Query: 183 MILGVIAKYGTDFATGYVIEYTGEAIRELSMEERMTVCNMSIEGGARAGLIAPDETTFNY 242 + I + + + ++ Sbjct: 171 GKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVEL 230 Query: 243 LRGRQYVPQGAAYEAAVETWKE--LVSDEGAQYDTVVEFDVETLIPQVTWGTSPGMGTNI 300 L + + + E V Sbjct: 231 LAYS----CLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAV------------ 274 Query: 301 TSTVPNPADFTTENERKAAEKALEYMDLVPGTPISEIGIDYVFIGSCTNGRIEDLRAAAE 360 P + + Sbjct: 275 --AAYVDIA-----------------RRHGLDPAQMALAFVRRQPFVASTLLGATTMDQL 315 Query: 361 VAKGHKVSDKVTAIVVPGSGRVKMQAEKEGLDKVFTEAGFEWREAGCSMCLAMNPDVLQP 420 Sbjct: 316 375 Query: 421 GQRCASTSNRNFEGRQGR-GGRTHLVSPAMAAAAAIKGHFTDVRD 464 Sbjct: 376 420 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.133 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 36,708,358 Number of extensions: 1501182 Number of successful extensions: 2637 Number of sequences better than 1.0e-05: 40 Number of HSP's gapped: 2546 Number of HSP's successfully gapped: 47 Length of query: 474 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 364 Effective length of database: 25,691,691 Effective search space: 9351775524 Effective search space used: 9351775524 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 118 (50.1 bits)