BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0010.p (208 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0110 [E] COG0066 3-isopropylmalate dehydratase small subunit 231 8e-61 STM0330 [E] COG0066 3-isopropylmalate dehydratase small subunit 143 2e-34 DR1784 [E] COG0066 3-isopropylmalate dehydratase small subunit 68 9e-12 TM0555 [E] COG0066 3-isopropylmalate dehydratase small subunit 67 2e-11 MK1206 [E] COG0066 3-isopropylmalate dehydratase small subunit 66 3e-11 >STM0110 [E] COG0066 3-isopropylmalate dehydratase small subunit Length = 346 Score = 231 bits (588), Expect = 8e-61, Method: Compositional matrix adjust. Identities = 10/199 (5%), Positives = 38/199 (19%), Gaps = 3/199 (1%) Query: 8 IMEAFKKLTGIVAPVDRVNVDTDAIIPKQFLKRIERTGFGQFLFYEWRF-DEAGND-NAA 65 + + + + T + + + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 66 FEMNKPRYKGASILISRANFGCGSPREHAPWAIMDYGFRVVIAPSYADIFYNNCFKNGIL 125 + Y G + + + + + + + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 126 PIKLSESQVDELFKRTAEHDGYKLTVDLENNLLSDDHGLSITFELDEHRRQFLLQGLDDI 185 + + + KL ++ + L E++R ++ + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLL-DTLDALAEYQRAGKIRYIGVS 179 Query: 186 GLTLQHADEIAAYEERHAA 204 T ++H Sbjct: 180 NETAFGVMRYLHLADKHDL 198 >STM0330 [E] COG0066 3-isopropylmalate dehydratase small subunit Length = 346 Score = 143 bits (360), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 15/197 (7%), Positives = 35/197 (17%), Gaps = 1/197 (0%) Query: 9 MEAFKKLTGIVAPVDRVNVDTDAIIPKQFLKRIERTGFGQFLFYEWRFDEAGNDNAAFEM 68 M+ + + + + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 69 NKPRYKGASILISRANFGCGSPREHAPWAIMDYGFRVVIAPSYADIFYNNCFKNGILPIK 128 + + + + + I P A N + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 129 LSESQVDELFKRTAEHDGYKLTVDLENNLLSDDHGLSITFELDEHRRQFLLQGLDDIGLT 188 L +D + + S S+ LD + IG++ Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAV-SLLDTLDALAEYQRAGKIRYIGVS 179 Query: 189 LQHADEIAAYEERHAAK 205 + A + Y Sbjct: 180 NETAFGVMRYLHLADKH 196 >DR1784 [E] COG0066 3-isopropylmalate dehydratase small subunit Length = 346 Score = 68.2 bits (165), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 2/135 (1%), Positives = 17/135 (12%), Gaps = 18/135 (13%) Query: 24 RVNVDTDAIIPKQFLKRIERTGFGQFLFYEWRFDEAGNDNAAFEMNKPRYKGASILISRA 83 + + A + Sbjct: 9 SSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI-----------NLIDVAEMYPVPP 57 Query: 84 NFGCGSPREHAPWAIMDYGFRVVIAPSYADIFYNNCFKNGILPIKLSESQVDELFKRTAE 143 E + + + + + + + + + Sbjct: 58 RPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQ--ALDRKNIR---- 111 Query: 144 HDGYKLTVDLENNLL 158 + ++ Sbjct: 112 -EALHDSLKRLQTDY 125 >TM0555 [E] COG0066 3-isopropylmalate dehydratase small subunit Length = 346 Score = 67.0 bits (162), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 7/135 (5%), Positives = 21/135 (15%), Gaps = 18/135 (13%) Query: 26 NVDTDAIIPKQFLKRIERTGFGQFLFYEWRFDEAGNDNAAFEMNKPRYKGASILISRANF 85 + + + + + + + Y Sbjct: 15 TLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEM---------YPV-PPRPETQGL 64 Query: 86 GCGSPREHAPWAIMDYGFRVVIAPSYADIFYNNCFKNGILPIKLSESQVDELFKRTAEHD 145 + S + + + + + L HD Sbjct: 65 TETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIR--EAL------HD 116 Query: 146 GYKLTVDLENNLLSD 160 K +L Sbjct: 117 SLKRLQTDYLDLYQV 131 >MK1206 [E] COG0066 3-isopropylmalate dehydratase small subunit Length = 346 Score = 66.2 bits (160), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 12/133 (9%), Positives = 20/133 (15%), Gaps = 17/133 (12%) Query: 26 NVDTDAIIPKQFLKRIERTGFGQFLFYEWRFDEAGNDNAAFEMNKPRYKGASILISRANF 85 + E Q + A + R Sbjct: 13 VSTLGLGTMTFGEQNSEADAHAQLDY-------AVAQGINLI----DVAEMYPVPPRPET 61 Query: 86 GCGSPREHAPWAIMDYGFRVVIAPSYADIFYNNCFKNGILPIKLSESQVDELFKRTAEHD 145 + W +I S N K L + E Sbjct: 62 QGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREA------LH 115 Query: 146 GYKLTVDLENNLL 158 + + L Sbjct: 116 DSLKRLQTDYLDL 128 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.140 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,617,586 Number of extensions: 661383 Number of successful extensions: 1718 Number of sequences better than 1.0e-05: 20 Number of HSP's gapped: 1705 Number of HSP's successfully gapped: 20 Length of query: 208 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 106 Effective length of database: 26,614,363 Effective search space: 2821122478 Effective search space used: 2821122478 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 113 (48.1 bits)