BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0007.p (479 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value alr0092 [M] COG0860 N-acetylmuramoyl-L-alanine amidase 130 5e-30 alr0093 [M] COG0860 N-acetylmuramoyl-L-alanine amidase 127 4e-29 BS_yrvJ_2 [M] COG0860 N-acetylmuramoyl-L-alanine amidase 126 9e-29 slr1910 [M] COG0860 N-acetylmuramoyl-L-alanine amidase 124 3e-28 slr1744 [M] COG0860 N-acetylmuramoyl-L-alanine amidase 114 3e-25 >alr0092 [M] COG0860 N-acetylmuramoyl-L-alanine amidase Length = 359 Score = 130 bits (327), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 0/194 (0%), Positives = 0/194 (0%), Gaps = 4/194 (2%) Query: 287 NAASTDDPATLPGNNGRKLVVLDAGHGAKDSGAVGVTGKYEKNFNXXXXXXXXXXXXQEN 346 Sbjct: 435 - 493 Query: 347 KVDVVLTRSDDTFLELKERAGMANNLKADLFISVHANXX--XXXXXXXXETYYQRAESKA 404 Sbjct: 494 - 552 Query: 405 LANVMHKYLVQATGLSDRGVRYGNFHVIRETTMPAVLLEVGYLSNKGDEALLFSEDLQNK 464 Sbjct: 553 612 Query: 465 VAAGMVAGIKEYLK 478 Sbjct: 613 626 >alr0093 [M] COG0860 N-acetylmuramoyl-L-alanine amidase Length = 359 Score = 127 bits (319), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 0/186 (0%), Positives = 0/186 (0%), Gaps = 4/186 (2%) Query: 294 PATLPGNNGRKLVVLDAGHGAKDSGAVGVTGKYEKNFNXXXXXXXXXXXXQENKVDVVLT 353 Sbjct: 443 --- 499 Query: 354 RSDDTFLELKERAGMANNLKADLFISVHANXXXXXXXXXXETYYQRAESKALANVMHKYL 413 Sbjct: 500 559 Query: 414 VQATG-LSDRGVRYGNFHVIRETTMPAVLLEVGYLSNKGDEALLFSEDLQNKVAAGMVAG 472 Sbjct: 560 619 Query: 473 IKEYLK 478 Sbjct: 620 625 >BS_yrvJ_2 [M] COG0860 N-acetylmuramoyl-L-alanine amidase Length = 198 Score = 126 bits (316), Expect = 9e-29, Method: Compositional matrix adjust. Identities = 65/185 (35%), Positives = 105/185 (56%), Gaps = 5/185 (2%) Query: 298 PGNNGR---KLVVLDAGHGAKDSGAVGVTGKYEKNFNXXXXXXXXXXXXQENKVDVVLTR 354 P NG K +++D GHG KDSG +G +GK+EKN + DV +TR Sbjct: 15 PSGNGSLNNKTIIVDPGHGGKDSGTIGYSGKFEKNLTIKTAKLLASKL-RSAGADVYVTR 73 Query: 355 SDDTFLELKERAGMANNLKADLFISVHANXXXXXXXXXXETYYQR-AESKALANVMHKYL 413 DDTF+ L+ R ++ AD FIS+H + YY A+ + LA+ +H + Sbjct: 74 QDDTFVSLQSRVSTSHYRNADAFISIHYDSYADTSTRGSTAYYYSPAKDQELASDVHSEV 133 Query: 414 VQATGLSDRGVRYGNFHVIRETTMPAVLLEVGYLSNKGDEALLFSEDLQNKVAAGMVAGI 473 V+ + + DRGV +G+++V+RE PA+L E+GY+S+ +EA++ S Q KV G+ +G+ Sbjct: 134 VKRSSIPDRGVLFGDYYVLRENRQPAMLYELGYVSHPQEEAIVHSNSYQEKVTDGIESGL 193 Query: 474 KEYLK 478 ++Y + Sbjct: 194 EKYFQ 198 >slr1910 [M] COG0860 N-acetylmuramoyl-L-alanine amidase Length = 338 Score = 124 bits (312), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 94/315 (29%), Positives = 147/315 (46%), Gaps = 35/315 (11%) Query: 182 FSENRLTIAMDGNSQPVVSKVTGPDRIVIDLPNATFSDVFGTGQVLSPDLNGSLAVTDYP 241 ++RL I D + +P V+ + P R+V+DLP P + L T Y Sbjct: 41 LRDSRLDIITDEDVRPQVNVLRNPTRVVVDLPGIEHR---------GPTIYKPL--TQY- 88 Query: 242 DVSGVRYSLYSTNPYTVRFVIDLNN---AKNYSVNVSGDVSKLVVVDL------------ 286 V R ++ N + R V++L K + V V G L Sbjct: 89 -VKEARVGRWNNN--STRIVLELTEPFTVKPWEVQVRGLAPNRWYARLPTILQPSEYSLP 145 Query: 287 -NAASTDDPATLPGNNGRKLVVLDAGHGAKDSGAVGVTGKYEKNFNXXXXXXXXXXXXQE 345 + + PA P R VVLDAGHG +D GA+G G EK+ ++ Sbjct: 146 QETVAVNVPAPAPRPLRRFTVVLDAGHGGQDPGAIGQRGIREKDV-VLAITRGVARELEK 204 Query: 346 NKVDVVLTRSDDTFLELKERAGMANNLKADLFISVHANXXXXXXXXX--XETYYQRAESK 403 ++VV+TR+ D F+ L+ R A +AD+F+S+HAN ETYY + + Sbjct: 205 QGIEVVMTRNSDIFVSLQGRVQRAAAARADIFVSIHANSIGLGRPEVNGVETYYFQT-GR 263 Query: 404 ALANVMHKYLVQATGLSDRGVRYGNFHVIRETTMPAVLLEVGYLSNKGDEALLFSEDLQN 463 +LA +H+ ++Q + DR VR F+V+R T+MP+ L+EVG+++ D L + Q Sbjct: 264 SLAQTIHRSILQRLNVRDRRVRQARFYVLRRTSMPSTLVEVGFVTGSQDSRNLSNPRFQQ 323 Query: 464 KVAAGMVAGIKEYLK 478 ++A + AGI +YLK Sbjct: 324 QMAEAIAAGIVQYLK 338 >slr1744 [M] COG0860 N-acetylmuramoyl-L-alanine amidase Length = 649 Score = 114 bits (285), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 62/179 (34%), Positives = 97/179 (54%), Gaps = 5/179 (2%) Query: 302 GRKLVVLDAGHGAKDSGAVGVTGKYEKNFNXXXXXXXXXXXXQENKVDVVLTRSDDTFLE 361 G +LVV+D GHG KD GA+G+ G EK+ Q+ V V++TR+ D F+ Sbjct: 472 GGRLVVVDPGHGGKDPGAIGIRGVQEKDVVLAVSQYLQRYLEQQG-VRVLMTRTGDYFIS 530 Query: 362 LKERAGMANNLKADLFISVHANXXXXXXXXXX--ETYYQRAESKALANVMHKYLVQATGL 419 L+ R MAN ADLF+S+HAN E YY + L+ +H+ +V + + Sbjct: 531 LQGRTDMANRAGADLFVSIHANSMGMGRPDVNGFEIYYH--GNAGLSQAIHRNVVNSLNV 588 Query: 420 SDRGVRYGNFHVIRETTMPAVLLEVGYLSNKGDEALLFSEDLQNKVAAGMVAGIKEYLK 478 DR VR F+V+R + MP+ L+E+G+++ D L + Q ++A + G+ EYL+ Sbjct: 589 RDRRVRQARFYVLRNSRMPSTLVEMGFVTGNEDNYKLTDPNFQQQMAQAIARGVLEYLQ 647 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.132 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,060,892 Number of extensions: 1399924 Number of successful extensions: 4217 Number of sequences better than 1.0e-05: 48 Number of HSP's gapped: 4142 Number of HSP's successfully gapped: 48 Length of query: 479 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 369 Effective length of database: 25,691,691 Effective search space: 9480233979 Effective search space used: 9480233979 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 118 (50.1 bits)