BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0002.p (223 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value DR0289 [L] COG0177 Predicted EndoIII-related endonuclease 187 1e-47 aq_496 [L] COG0177 Predicted EndoIII-related endonuclease 185 4e-47 MA4014 [L] COG0177 Predicted EndoIII-related endonuclease 184 1e-46 CPn0707 [NU] COG1157 Flagellar biosynthesis/type III secretory p... 176 2e-44 CAC0676 [I] COG1183 Phosphatidylserine synthase 176 2e-44 >DR0289 [L] COG0177 Predicted EndoIII-related endonuclease Length = 225 Score = 187 bits (474), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 95/201 (47%), Positives = 123/201 (61%), Gaps = 1/201 (0%) Query: 4 AEVRHILDTIGDLFPDAHCELNHSNAFELTIAVLLSAQCTDATVNKVTEDLFQKYKTPLD 63 A +L +G L+PDA EL + FEL +A +LSAQ TD +VN T LF Y Sbjct: 16 ARAPQVLSALGRLYPDARTELVFNTPFELLVATVLSAQATDVSVNAATPALFAAYPDAHA 75 Query: 64 YISVPIEELESDIRRIGLYRNKAKHIQSLCSILIEQYGGEVPQAHDLLVTLPGVGRKTAN 123 +++E IR IGLYR KAK++ +L +L+E++GGEVP D +V LPG GRKTAN Sbjct: 76 LSQATADDIEPYIRSIGLYRGKAKNLAALARLLVERHGGEVPNDFDAVVALPGAGRKTAN 135 Query: 124 VVVSNAFGVPAIAVDTHVERVAKRLALAGWKDSVLEVEKKLMKAVPRDEWTLTHHRLIFF 183 VV+SNA+ PAIAVDTHV R+A+RL L+ + + +VE L K PRD W HH LI Sbjct: 136 VVLSNAYDYPAIAVDTHVGRLARRLGLS-VQTNPDKVEADLQKLFPRDRWVFLHHALILH 194 Query: 184 GRYHCKAQNPKCQVCPLLDVC 204 GR C A+ P+C C L C Sbjct: 195 GRRVCHARKPQCPSCELASFC 215 >aq_496 [L] COG0177 Predicted EndoIII-related endonuclease Length = 232 Score = 185 bits (470), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 91/205 (44%), Positives = 136/205 (66%), Gaps = 1/205 (0%) Query: 8 HILDTIGDLFPDAHCELNHSNAFELTIAVLLSAQCTDATVNKVTEDLFQKYKTPLDYISV 67 I+ + ++ + EL + NAF+L + +L+AQ +D VNKV+++ F+KYKTP D Sbjct: 26 EIVKRLEKVYLNPRLELEYENAFQLLVMAILAAQESDKVVNKVSKEFFKKYKTPQDIARA 85 Query: 68 PIEELESDIRRIGLYRNKAKHIQSLCSILIEQYGGEVPQAHDLLVTLPGVGRKTANVVVS 127 +EELE D++ I YR KAK I+ C LIE Y GEVP++ LV LPGVGRKTAN+V+ Sbjct: 86 NLEELEEDLKHINFYRRKAKLIKECCEKLIELYKGEVPKSVGELVKLPGVGRKTANMVIG 145 Query: 128 NAFGVPAIAVDTHVERVAKRLALAGWKDSVLEVEKKLMKAVPRDEWTLTHHRLIFFGRYH 187 A+ +PAI VD HV RV +R++L+ K+ ++E +L + VP++ WT L+ G+ Sbjct: 146 GAYNLPAIIVDRHVHRVVERISLSKQKNPD-KMEMELSEIVPQELWTKFSLLLLNHGKTI 204 Query: 188 CKAQNPKCQVCPLLDVCREGKKRMK 212 CKA+NP+C+ CP+LD+C G+K +K Sbjct: 205 CKARNPECEKCPILDLCEYGQKNLK 229 >MA4014 [L] COG0177 Predicted EndoIII-related endonuclease Length = 256 Score = 184 bits (466), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 94/203 (46%), Positives = 134/203 (66%), Gaps = 6/203 (2%) Query: 7 RHILDTIGDL----FPDAHCELNHSNAFELTIAVLLSAQCTDATVNKVTEDLFQKYKTPL 62 RH D++ L +PDA LN+SN EL IA +LSAQ TD +N+VTE+LF+KY+T Sbjct: 46 RHNFDSVWALLKAEYPDAKPSLNYSNPLELLIATVLSAQSTDVQINRVTENLFKKYRTAE 105 Query: 63 DYISVPIEELESDIRRIGLYRNKAKHIQSLCSILIEQYGGEVPQAHDLLVTLPGVGRKTA 122 DY I ELE DI G Y+NKAK+I++ +++E++GGEVP+ LVTLPGVGRKTA Sbjct: 106 DYAGADIRELEIDIYSTGFYKNKAKNIKAAAQMIVERFGGEVPKTMKELVTLPGVGRKTA 165 Query: 123 NVVVSNAFGV-PAIAVDTHVERVAKRLALAGWKDSVLEVEKKLMKAVPRDEWTLTHHRLI 181 N+V++ AFGV IAVDTHV+RV++RL D ++E+ L+ +++ LI Sbjct: 166 NIVLARAFGVIEGIAVDTHVKRVSRRLGFTRHSDPE-KIEQDLITLARKEDLDSISMTLI 224 Query: 182 FFGRYHCKAQNPKCQVCPLLDVC 204 + GR C+A+ P+C VC + ++C Sbjct: 225 YHGRKVCRARKPRCYVCVVKELC 247 >CPn0707 [NU] COG1157 Flagellar biosynthesis/type III secretory pathway ATPase Length = 211 Score = 176 bits (447), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 98/209 (46%), Positives = 130/209 (62%), Gaps = 1/209 (0%) Query: 1 MKAAEVRHILDTIGDLFPDAHCELNHSNAFELTIAVLLSAQCTDATVNKVTEDLFQKYKT 60 M A+ IL + + P ELN S+ FEL IAVLLSAQ TD +VNK T L+ T Sbjct: 1 MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANT 60 Query: 61 PLDYISVPIEELESDIRRIGLYRNKAKHIQSLCSILIEQYGGEVPQAHDLLVTLPGVGRK 120 P + + +E +++ I+ IGLY +KA++I C IL+EQ+ GEVP+ L LPGVGRK Sbjct: 61 PAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRK 120 Query: 121 TANVVVSNAFGVPAIAVDTHVERVAKRLALAGWKDSVLEVEKKLMKAVPRDEWTLTHHRL 180 TANVV++ AFG P IAVDTH+ RV R A K+ V +VE+KL+K VP + HH L Sbjct: 121 TANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKN-VEQVEEKLLKVVPAEFKVDCHHWL 179 Query: 181 IFFGRYHCKAQNPKCQVCPLLDVCREGKK 209 I GRY C A+ P+C C + D+C +K Sbjct: 180 ILHGRYTCIARKPRCGSCIIEDLCEYKEK 208 >CAC0676 [I] COG1183 Phosphatidylserine synthase Length = 211 Score = 176 bits (447), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 98/209 (46%), Positives = 130/209 (62%), Gaps = 1/209 (0%) Query: 1 MKAAEVRHILDTIGDLFPDAHCELNHSNAFELTIAVLLSAQCTDATVNKVTEDLFQKYKT 60 M A+ IL + + P ELN S+ FEL IAVLLSAQ TD +VNK T L+ T Sbjct: 1 MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANT 60 Query: 61 PLDYISVPIEELESDIRRIGLYRNKAKHIQSLCSILIEQYGGEVPQAHDLLVTLPGVGRK 120 P + + +E +++ I+ IGLY +KA++I C IL+EQ+ GEVP+ L LPGVGRK Sbjct: 61 PAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRK 120 Query: 121 TANVVVSNAFGVPAIAVDTHVERVAKRLALAGWKDSVLEVEKKLMKAVPRDEWTLTHHRL 180 TANVV++ AFG P IAVDTH+ RV R A K+ V +VE+KL+K VP + HH L Sbjct: 121 TANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKN-VEQVEEKLLKVVPAEFKVDCHHWL 179 Query: 181 IFFGRYHCKAQNPKCQVCPLLDVCREGKK 209 I GRY C A+ P+C C + D+C +K Sbjct: 180 ILHGRYTCIARKPRCGSCIIEDLCEYKEK 208 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,731,001 Number of extensions: 647415 Number of successful extensions: 10459 Number of sequences better than 1.0e-05: 4658 Number of HSP's gapped: 5795 Number of HSP's successfully gapped: 4658 Length of query: 223 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 121 Effective length of database: 26,614,363 Effective search space: 3220337923 Effective search space used: 3220337923 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 114 (48.5 bits)