BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0542.0021.p (304 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM1295 [OU] COG0616 Periplasmic serine proteases (ClpP class) 94 2e-19 TM1295 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 94 2e-19 PA1415 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 69 7e-12 mll0231 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 56 9e-08 CAC1424 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 54 4e-07 >STM1295 [OU] COG0616 Periplasmic serine proteases (ClpP class) Length = 618 Score = 94.4 bits (233), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 9/222 (4%), Positives = 44/222 (19%), Gaps = 44/222 (19%) Query: 45 FYLVLGQKKAVLIDAGFGFGDIRPIIRSITDLPVMLVLTHGDPDHSYGSEYFGDVWLYQP 104 + ++ + ++L ++ D + G + Sbjct: 26 VLNLFFIFLVLVGVGIWMQIGNGSNSEQTARGALLLDISGVIVDKPSTNHRLGALGRQLF 85 Query: 105 DYGQIMGFDTKHERQFAAELGVKYLKENNPEAIERFDSKAFAEKSLLRVLTPHFVKDGEL 164 + ++ F + + + Sbjct: 86 GA----------------------------SSDRLQENSLFDIVNA-------IRQAKDD 110 Query: 165 FDLGGKTLEVVYTPGHSYGHIMLLDRAKKRLFSGDMI-CDYVIIYFFESDRNAPFSMALD 223 ++ G L++ G + + +A + Y + + Sbjct: 111 RNITGIVLDLKNFTGADQPSMRYIGKALREFRDSGKPVFAVGENYSQGQ---YYLASFAN 167 Query: 224 SWKKIKRLEKDIKDIYSSH----GQYPITMEWVDAYIECFSG 261 + + ++++ ++ + Sbjct: 168 KIWLSPQGQV-DLHGFATNGLYYKTLLDKLKVSTHVFRVGTY 208 >TM1295 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 218 Score = 94.4 bits (233), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 72/222 (32%), Positives = 104/222 (46%), Gaps = 44/222 (19%) Query: 45 FYLVLGQKKAVLIDAGFGFGDIRPIIRSITDLPVMLVLTHGDPDHSYGSEYFGDVWLYQP 104 YLV+G++KA+L+D G G GD++ +RSIT+ PV +VLTH DH + F V+L Sbjct: 26 MYLVVGEEKALLVDTGMGEGDLKGFVRSITEKPVEVVLTHAHWDHIMQANQFEKVYLNHR 85 Query: 105 DYGQIMGFDTKHERQFAAELGVKYLKENNPEAIERFDSKAFAEKSLLRVLTPHFVKDGEL 164 D+ + IE F E L VK+G+ Sbjct: 86 DF----------------------------QIIELFKINVNYENFL-------NVKEGDR 110 Query: 165 FDLGGKTLEVVYTPGHSYGHIMLLDRAKKRLFSGDMI-CDYVIIYFFESDRNAPFSMALD 223 FDLGG+TLEV+ PGH+ G I+LLDR + LFSGD + + ++ P L Sbjct: 111 FDLGGRTLEVIEVPGHTPGSIVLLDRENRLLFSGDSVGAGHTWMHLPGC---LPLREYLK 167 Query: 224 SWKKIKRLEKDIKDIYSSH----GQYPITMEWVDAYIECFSG 261 S KK+K + + IY H P +++D I+ G Sbjct: 168 SLKKLKETDS-FEKIYHGHLTGTQLKPFGPDYLDDLIKAVEG 208 >PA1415 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 242 Score = 69.3 bits (168), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 46/163 (28%), Positives = 81/163 (49%), Gaps = 7/163 (4%) Query: 45 FYLVLGQKKAVLIDAGFGFGDIRPIIRSITDLPVMLVLTHGDPDHSYGSEYFGDVWLYQP 104 + V G+++ VL+D+G G + + +T+ P++ V +H DH G F + L P Sbjct: 35 MWHVQGRERDVLVDSGSGLVSLCEQLPWLTERPLLAVASHTHFDHIAGHHEFAER-LAHP 93 Query: 105 DYGQIMGFDTKHERQFAAELGVKYLKENNPEAIERFDSKAFAEKSLLRVLTPHFVKDGEL 164 +I+ A +G + E +PE +AE + + +G++ Sbjct: 94 AEAEILAAPDGDNTLARAYVGDEMF-EAHPEC-----PLCYAEYRVRAAPATRLIDEGDV 147 Query: 165 FDLGGKTLEVVYTPGHSYGHIMLLDRAKKRLFSGDMICDYVII 207 DLG + L+V++TPGHS G I L + A + LFSGD++ D ++ Sbjct: 148 LDLGDRVLQVLHTPGHSPGGISLWEAATQTLFSGDIVYDGPLV 190 >mll0231 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 253 Score = 55.8 bits (133), Expect = 9e-08, Method: Compositional matrix adjust. Identities = 47/184 (25%), Positives = 83/184 (45%), Gaps = 13/184 (7%) Query: 29 KLDDDLYIITEAGLVHFYL-----VLGQKKAVLIDAGFGFGDIRPIIRSITDLPVMLVLT 83 ++ D + +I E + F+ V G+ + +L D G G +R + +T+ + V + Sbjct: 17 RMADGVTLIHEPWIKPFFRCNIWHVRGRDRDLLFDTGLGHFSLRTHVPLVTERKLTCVAS 76 Query: 84 HGDPDHSYGSEYFGDVWLYQPDYGQIMGFDTKHERQFAAELGVKYLKENNPEAIERFDSK 143 H DH F D ++ P +I+ D ++E A + + P+ E Sbjct: 77 HTHFDHIGCHHEFPDRGVH-PAEAKILA-DPRNEWTVADRYATDDMFDGLPQGWES---- 130 Query: 144 AFAEKSLLRVLTPHFVKDGELFDLGGKTLEVVYTPGHSYGHIMLLDRAKKRLFSGDMICD 203 + +L + G++ DLG + EV++TPGHS G I L ++ L SGD+I D Sbjct: 131 --SRYRILPAPADRLLDHGDVVDLGDRAFEVIHTPGHSPGGIALYEKKTGILLSGDIIYD 188 Query: 204 YVII 207 +I Sbjct: 189 GPLI 192 >CAC1424 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 249 Score = 53.5 bits (127), Expect = 4e-07, Method: Compositional matrix adjust. Identities = 62/241 (25%), Positives = 102/241 (42%), Gaps = 37/241 (15%) Query: 26 KVQKLDDDLYIITEAGL----VHFYLVLGQKKAVLIDAGFGFGDIRPIIRSITDLPVMLV 81 K++KL+ YI T + + +L+ + + +ID G + PI I D + ++ Sbjct: 2 KLKKLNKSTYIFTYDNIKEYSTNVFLIKKKNRVYVIDTFCGTKSMEPITNEIGDKEICVI 61 Query: 82 LTHGDPDHSYGSEYFGDVWLYQPDYGQIMGFDTKHERQFAAELGVKYLKENNPEAIERFD 141 TH DH +G+ F D + K R+ E LKEN ER+ Sbjct: 62 NTHFHWDHIFGNSAFKDAIIISH----------KKCRKILEESWDSQLKEN-----ERY- 105 Query: 142 SKAFAEKSLLRVLTPHFVKDGE-LFDLGGKTLEVVYTPGHSYGHIMLLDRAKKRLFSGDM 200 AEK + P+ E +F+ G +E+ Y+PGH+ I + D + L+ GD Sbjct: 106 VLGSAEKCI-----PNLTFKSEIMFEEDG--IEIFYSPGHTEDSISIFDHESRILYVGDN 158 Query: 201 ICDYVIIYFFESDRNAPFSMALDSWKKIKRLEKDIKDIYSSHGQYPITMEWVDA--YIEC 258 + + I+Y SD +A S K +E D I++ H T + +D Y+E Sbjct: 159 L-EKPIVYVENSDIDAYMSTLK------KYIEYDADKIFAGHTLNLTTEDILDTINYLEA 211 Query: 259 F 259 Sbjct: 212 L 212 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.142 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,927,876 Number of extensions: 967886 Number of successful extensions: 7884 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 7889 Number of HSP's successfully gapped: 9 Length of query: 304 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 198 Effective length of database: 26,153,027 Effective search space: 5178299346 Effective search space used: 5178299346 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 115 (48.9 bits)