BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0542.0019.p (331 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin1217 [GEPR] COG0477 Permeases of the major facilitator superf... 182 6e-46 SPy1856 [GEPR] COG0477 Permeases of the major facilitator superf... 160 4e-39 L101912 [GEPR] COG0477 Permeases of the major facilitator superf... 156 5e-38 lin1658 [GEPR] COG0477 Permeases of the major facilitator superf... 49 8e-06 >lin1217 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 401 Score = 182 bits (462), Expect = 6e-46, Method: Compositional matrix adjust. Identities = 90/326 (27%), Positives = 170/326 (52%) Query: 1 MEVIGRRKXXXXXXXXXXXXXXXXXXVYDLNMLLVVRFVHGVIFGVATTAIPTVIMGMIP 60 +E+ GR++ + + ++ ++RF++G +G +TA ++ IP Sbjct: 68 LELFGRKQVLRFGILFFLVTTMAYLYMPTIAIMFIIRFLNGFAYGTTSTATNAIVTAYIP 127 Query: 61 RERRGEGVGYFTLSGTMASAIGPFLGLVIREQANFTTMFVVCAVFYVISIIVTAFADIPE 120 R GEG+ Y+ LS ++A+AIGPF+G+++ + +F T+ + V +++ ++ + + Sbjct: 128 NSRNGEGINYYGLSTSLAAAIGPFIGMILLSKTSFYTIIIFSTVIVLLTALLCFYLPVKN 187 Query: 121 VVMTKEQLEVVKGFRLQDFFEKTAMPVSIVMLFVGMAYASILAFINSYALSLSLMDAAAF 180 +V+TKE ++ + ++ F E +P++ + +G++Y+S+L F+ SYA ++L+ A F Sbjct: 188 IVLTKEHRTALQTWTVKSFIETKVIPITFIAFLMGISYSSVLTFLASYAREINLVSAGTF 247 Query: 181 FFVIYSAFIIISRLFTGRLLDNRGDNIVMYPAXXXXXXXXXXISKAGDSFTXXXXXXXXX 240 FFV+Y+ I +R +G+L D +G+ VMYP+ +S A S Sbjct: 248 FFVVYALVITFTRPMSGKLFDAKGEKYVMYPSYLFLAVGLVVLSTATSSIVLLISGGLIG 307 Query: 241 XXXXTIMSCAQVIAANNSPQHRMGIATSTFFFCADLGVGAGSFFAGEIVSMTGFRDMYMV 300 T MS Q + HR+GI ST+F DLG+G G + GEI + F+ +Y++ Sbjct: 308 LGYGTFMSNGQAVCLKVCEPHRIGIGLSTYFIGLDLGLGIGPYIMGEIHHVLSFQGIYII 367 Query: 301 MAIIVALSILLYYILHGRKTASDKHS 326 I + + +Y L +K +S H Sbjct: 368 AGAIAFVCVFIYMFLSRKKVSSGAHE 393 >SPy1856 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 431 Score = 160 bits (404), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 97/321 (30%), Positives = 160/321 (49%), Gaps = 10/321 (3%) Query: 1 MEVIGRRKXXXXXXXXXXXXXXXXXXVYDLNMLLVVRFVHGVIFGVATTAIPTVIMGMIP 60 +EV GRR + ++M+ +VRF++G +GV +TA T++ IP Sbjct: 101 LEVFGRRLVLRGGAIFYLLTTLAYFYMPTISMMYLVRFLNGFGYGVVSTATNTIVTAYIP 160 Query: 61 RERRGEGVGYFTLSGTMASAIGPFLGLVIRE--QANFTTMFVVCAVFYVISIIVTAFA-D 117 +RGEG+ ++ LS ++A+AIGPF+G + + +F + V+C+V + ++V AFA Sbjct: 161 ARKRGEGINFYGLSTSLAAAIGPFVGTFMLDNLHIDFRMIIVLCSVL-IGCVVVGAFAFP 219 Query: 118 IPEVVMTKEQLEVVKGFRLQDFFEKTAMPVSIVMLFVGMAYASILAFINSYALSLSLMDA 177 + + + EQL K + + F EK A+ ++ + +G+AYAS+L F Y + L Sbjct: 220 VKNMSLNAEQLAKTKSWTVDSFIEKKALFITAIAFLMGIAYASVLGFQKLYTSEIHLTTV 279 Query: 178 AAFFFVIYSAFIIISRLFTGRLLDNRGDNIVMYPAXXXXXXXXXXISKAGDSFTXXXXXX 237 A+FFV+Y+ I I+R GRL+D +GD V+YP+ + + Sbjct: 280 GAYFFVVYALIITITRPAMGRLMDAKGDKWVLYPSYLFLAMGLFLLGSVSSGGSYLLSGA 339 Query: 238 XXXXXXXTIMSCAQVIAANNSPQHRMGIATSTFFFCADLGVGAGSFFAGEIVSM------ 291 T MSC Q + +HR A ST+ DLG+GAG + G I + Sbjct: 340 LIGFGYGTFMSCGQAASIQGVDEHRFNTAMSTYMIGLDLGLGAGPYLLGLIKDLALGSGV 399 Query: 292 TGFRDMYMVMAIIVALSILLY 312 FR ++ + A+I + LLY Sbjct: 400 ASFRHLFWLAAVIPLICTLLY 420 >L101912 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 406 Score = 156 bits (394), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 95/311 (30%), Positives = 155/311 (49%), Gaps = 16/311 (5%) Query: 29 DLNMLLVVRFVHGVIFGVATTAIPTVIMGMIPRERRGEGVGYFTLSGTMASAIGPFLGLV 88 ++ +L ++R + G FG+ TA T+ ++P RRGEG+GY+ LS T+A+A+GP L ++ Sbjct: 97 NIPLLFLIRVIQGAGFGIGATASGTIAGHVVPASRRGEGIGYYALSVTLAAAVGPALSIM 156 Query: 89 IREQANFTTMFVVCAVFYVISIIVTAFADIPEVVMTKEQLEVV---KGFRLQDFFEKTAM 145 I F ++ + VI+ I+ F + E + + KG ++ + EK+A+ Sbjct: 157 IYSSLGFGSLLGIALGLLVITFILIFFVRVKEFSAEERAYALAHEPKG--VERYIEKSAL 214 Query: 146 PVSIVMLFVGMAYASILAFINSYALSLSLMDAAAFFFVIYSAFIIISRLFTGRLLDNRGD 205 P+SI+ G ++IL + S++ L + A + FF +Y+ I +SR FTGRL D +GD Sbjct: 215 PISIIAFLAGFIDSAILTGMGSFSTDLKIPLAGSLFFTMYAVLIFVSRPFTGRLFDTKGD 274 Query: 206 NIVMYPAXXXXXXXXXXISKAG------DSFTXXXXXXXXXXXXXTIMSCAQVIAANNSP 259 N + P + AG + Q IA +S Sbjct: 275 NWIFNPTFIFFAVAMLLVGLAGFFSPAIGFVVLLIAGGAFGLGYGGVAPFGQAIAIRDSS 334 Query: 260 QHRMGIATSTFFFCADLGVGAGSFFAGEIV-----SMTGFRDMYMVMAIIVALSILLYYI 314 + R+G+ATSTFF DLGVG G G I+ M GFR++Y+ A V + ++YY+ Sbjct: 335 KDRIGVATSTFFGFLDLGVGGGPIVLGAIIPMLGNGMLGFRNLYLYSAPAVVIVWIIYYL 394 Query: 315 LHGRKTASDKH 325 +HG+ S Sbjct: 395 IHGKHQKSSSK 405 >lin1658 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 489 Score = 49.3 bits (116), Expect = 8e-06, Method: Compositional matrix adjust. Identities = 27/87 (31%), Positives = 44/87 (50%) Query: 29 DLNMLLVVRFVHGVIFGVATTAIPTVIMGMIPRERRGEGVGYFTLSGTMASAIGPFLGLV 88 D ML+ R V + G+ + V++ + P ERRG +G L+ A AIGP L Sbjct: 99 DYTMLIAGRMVQAIGAGIVMPLLTVVVLNLFPMERRGRAMGLIGLAMNFAPAIGPTLSGW 158 Query: 89 IREQANFTTMFVVCAVFYVISIIVTAF 115 I +Q ++ +F + F ++ I+V F Sbjct: 159 IVQQYDWRNLFFIIIPFAILDIVVAIF 185 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.331 0.142 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,199,659 Number of extensions: 627865 Number of successful extensions: 2032 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 2028 Number of HSP's successfully gapped: 10 Length of query: 331 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 224 Effective length of database: 26,037,693 Effective search space: 5832443232 Effective search space used: 5832443232 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 116 (49.3 bits)