BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0542.0004.p (291 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc5105 [R] COG3618 Predicted metal-dependent hydrolase of the T... 176 4e-44 mlr7156 [R] COG3618 Predicted metal-dependent hydrolase of the T... 122 5e-28 AGl2610 [R] COG3618 Predicted metal-dependent hydrolase of the T... 105 7e-23 mll5767 [R] COG3618 Predicted metal-dependent hydrolase of the T... 77 2e-14 mlr1708 [R] COG3618 Predicted metal-dependent hydrolase of the T... 62 2e-09 >AGc5105 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 194 Score = 176 bits (446), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 0/278 (0%), Positives = 0/278 (0%), Gaps = 8/278 (2%) Query: 12 MYIDAHQHYWKIDRDDYGWITPEIPVLFRDYLPSDLEPHLHRHGISRTIVVQAAPTVEET 71 A + D + Sbjct: 15 KTTTAKLFAEEGVPVLDSDEVVHGLYRAEAVPLIDAAFPGTTISGMVDRQKLGDVLRKNP 74 Query: 72 EYILGLSGQAESIAGVXXXXXXXXASPTFKEQLRQLRGHRKFTGLRVMIQEMEDPGLLLT 131 L + A ++ L + + + Sbjct: 75 ANFNRLEEIVHPLVRNRQEAFLAKARID--DRAFALLDIPLLFETGAEGRVDKVVVVSCA 132 Query: 132 RPYIEALSYLADEDLPVDLLVLADQLPQLIEMLERVPGLRGVIDHLAKPPIASGQLEPWR 191 ++ + E +R + + + ++ Sbjct: 133 PEIQRERVLSRPGMTEEKFEMILARQMPDAEKRQRADFVVDSGNGVEAARDQVKEILQKL 192 Query: 192 SRMAQIAKHPGIYCKLSGMVTEADPQGWTQEDFTAYVHAVLELFGPERVMFGSDWPVCLM 251 Sbjct: 193 -- 250 Query: 252 AATYDEVVEVLRSALQGRLSAEEMEPVFGANAAGFYKL 289 Sbjct: 251 ---- 284 >mlr7156 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 297 Score = 122 bits (307), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 89/289 (30%), Positives = 132/289 (45%), Gaps = 12/289 (4%) Query: 8 GGVIMYIDAHQHYWKIDRD--DYGWITPEIPVLFRDYLPSDLEPHLHRHGISRT----IV 61 GG + ID H H IDR Y W+ +P L RD+ D R G+ R + Sbjct: 15 GGAPVIIDTHLHL--IDRSALRYPWLA-GVPQLNRDFSYEDYATEAQRVGVERVLHMEVD 71 Query: 62 VQAAPTVEETEYILGLSGQAESIAGVXXXXXXXXASPTFKEQLRQLRGHRKFTGLRVMIQ 121 V A ET + GLS QA S+ V F L + R + G R ++ Sbjct: 72 VDPADIEVETARVEGLSRQAGSML-VGAIASCRPEDADFPAYLERQRTNPFVKGFRRVLH 130 Query: 122 EMEDPGLLLTRPYIEALSYLADEDLPVDLLVLADQLPQLIEMLERVPGLRGVIDHLAKPP 181 + D L + + + L L DL+VL Q+P+ I + + P ++ V+DH P Sbjct: 131 VVPD-DLSEGAVFRDNIKRLGGIGLTFDLVVLPHQIPKAIALADLAPDVQFVLDHCGVPD 189 Query: 182 IASGQLEPWRSRMAQIAKHPGIYCKLSGMVTEADPQGWTQEDFTAYVHAVLELFGPERVM 241 I G PWR+ M++IA+ P + K+SG+V ADP WT YV + FG +R++ Sbjct: 190 IKGGAEHPWRAHMSEIARRPNVMAKISGVVAYADPVNWTVGTLRPYVEHTIAAFGWDRIV 249 Query: 242 FGSDWPVCLMAATYDEVVEVLRSALQGRLSAEEMEPVFGANAAGFYKLD 290 +GSDWPVC + V + L G S E + + NA ++L+ Sbjct: 250 WGSDWPVCTLGGGLSTWVAATHALLSG-CSIAERDRLLSGNARKLWRLE 297 >AGl2610 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 154 Score = 105 bits (263), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 0/280 (0%), Positives = 0/280 (0%), Gaps = 12/280 (4%) Query: 14 IDAHQHYWKIDRDDYGWITPEIPVLFRDYLPSDLEPHLHRHGISRTIVVQAAPTVEETEY 73 + ++ + D LP L P A+ E Sbjct: 21 GSQTANRTELPETIREFFLLL-NNRQIDALPQLLAPGALIVDGKGRGDTNAS--PRERLN 77 Query: 74 ------ILGLSGQAESIAGVXXXXXXXXASPTFKEQLRQLRGHRKFTGLRVMIQEMEDPG 127 + S + + + ++ P Sbjct: 78 DTLSFLRSPVVVLRSEPLFEGCRVTVVSPSGSDLRYYKLNF-QMISGKIVLLKILPAKP- 135 Query: 128 LLLTRPYIEALSYLADEDLPVDLLVLADQLPQLIEMLERVPGLRGVIDHLAKPPIASGQL 187 Sbjct: 136 195 Query: 188 EPWRSRMAQIAKHPGIYCKLSGMVTEADPQGWTQEDFTAYVHAVLELFGPERVMFGSDWP 247 Sbjct: 196 255 Query: 248 VCLMAATYDEVVEVLRSALQGRLSAEEMEPVFGANAAGFY 287 Sbjct: 256 - 294 >mll5767 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 297 Score = 77.4 bits (189), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 67/297 (22%), Positives = 121/297 (40%), Gaps = 25/297 (8%) Query: 13 YIDAHQHYWKIDRDDYGWITPEI-PVLFRDY--------LPSDLEPHLHRH---GISRTI 60 +ID H H W ++ + Y W+T + P F DY L LE +++ + + Sbjct: 6 FIDPHHHLWDLEVNYYPWLTDGVKPSAFGDYEAIRTNYRLDDYLEDARNQNLVKSVHLDV 65 Query: 61 VVQAAPTVEETEYILGLSGQAESIAGVXXXXXXXXASPTFKEQLRQLRGHRKFTGLRVMI 120 + V ET ++ ++ + G+ + P E L + + F G+R + Sbjct: 66 GFDPSDPVGETRWLQEIANKRGFPHGIVGYADL--SKPDVGELLDRHMEYPNFRGIRQSM 123 Query: 121 QEMEDPGLLL-TRPYIE-------ALSYLADEDLPVDLLVLADQLPQLIEMLERVPGLRG 172 DP RP + LA L DL + Q+ + +++ P ++ Sbjct: 124 NYHADPAKTYQARPEVSRTPEWRRGFRELAKRRLSFDLQLYYPQMEEFLQLAHDFPDVQI 183 Query: 173 VIDHLAKPPIASGQLEPWRSRMAQIAKHPGIYCKLSGMVTEADPQGWTQEDFTAYVHAVL 232 +++H W+ M ++A+ P + CK+SG+ WT YV + Sbjct: 184 ILNHTGMQVDGPKHFNAWKKGMHRLAQAPNVACKISGLGM--GDWSWTTASIRPYVEEAI 241 Query: 233 ELFGPERVMFGSDWPVCLMAATYDEVVEVLRSALQGRLSAEEMEPVFGANAAGFYKL 289 FG R MF +++PV + +++D VV + + EE +F NAA Y+L Sbjct: 242 AAFGVGRSMFATNFPVDKLFSSFDAVVNAFKE-ITRYYPHEERLALFHDNAARHYRL 297 >mlr1708 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Length = 295 Score = 61.6 bits (148), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 67/302 (22%), Positives = 122/302 (40%), Gaps = 40/302 (13%) Query: 14 IDAHQHYWKIDRDDYGWIT-PEIPVLF-------RDYLPSDLEPHLHRHGISRTIVVQAA 65 IDAH H W+ + D W+ P +P +F RDY + G+ + + VQ Sbjct: 5 IDAHFHIWR--QKDQPWLVGPMVPRIFGPYEPIRRDYPIGEFLQDQKGSGVEKAVYVQTN 62 Query: 66 PTVEETEYILGLSGQAESIAGV--XXXXXXXXASPTFKEQLRQLRGHRKFTGLRVMIQEM 123 E+ E + Q G + Q+ +L +R G+R+ + Sbjct: 63 WAKEDFEKEVAFLQQTADETGWPHAIVGYADMTVDDVRPQIDRLMKYRLLRGVRMQLHWH 122 Query: 124 EDPGLLLTR-------PYIEA-LSYLADEDLPVDLLVLADQLPQLIEMLERVPGLRGVID 175 E P P + A ++ L D L DL + Q+ + ++ P ++ Sbjct: 123 ETPAFRFAASADQVIDPKVRANVARLKDYGLSFDLQLFPAQMKDGVTLVGENPETNFILT 182 Query: 176 HLA-----KPPIASGQLEPWRSRMAQIAKHPGIYCKLSGM---VTEADPQGWTQEDFTAY 227 H +P + E W++ + ++ P Y KLSG+ V DP AY Sbjct: 183 HAGMLTGMEPEVT----ETWKAGLRTLSAAPNFYAKLSGLGTFVHRNDPA------LIAY 232 Query: 228 -VHAVLELFGPERVMFGSDWPVCLMAATYDEVVEVLRSALQGRLSAEEMEPVFGANAAGF 286 V +++ G + +MFGS++P+ + ++ E+++ R A+ +A E + +F A Sbjct: 233 IVDNAIDILGADHLMFGSNFPIEKLWTSHAELIKAHRDAVARHGAAAEAD-IFWNTAEKV 291 Query: 287 YK 288 Y+ Sbjct: 292 YR 293 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.139 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,086,392 Number of extensions: 906674 Number of successful extensions: 1368 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 1364 Number of HSP's successfully gapped: 5 Length of query: 291 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 186 Effective length of database: 26,268,361 Effective search space: 4885915146 Effective search space used: 4885915146 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 115 (48.9 bits)