BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0104.p (687 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ptsG_1 [G] COG1263 Phosphotransferase system IIC components, ... 518 e-147 BH0844_1 [G] COG1263 Phosphotransferase system IIC components, g... 506 e-143 SA0183_1 [G] COG1263 Phosphotransferase system IIC components, g... 446 e-125 STM2758_1 [G] COG1263 Phosphotransferase system IIC components, ... 337 5e-92 SA0233_1 [G] COG1263 Phosphotransferase system IIC components, g... 313 4e-85 >BS_ptsG_1 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 436 Score = 518 bits (1335), Expect = e-147, Method: Compositional matrix adjust. Identities = 259/428 (60%), Positives = 306/428 (71%), Gaps = 10/428 (2%) Query: 1 MFKKLFGVLQRVGKALMXXXXXXXXXXXXXXXXNMLVNPDFLQYVPALENDVVQAVATVL 60 MFK LFGVLQ++G+ALM N + N D +Q + L ND VQ VA V+ Sbjct: 1 MFKALFGVLQKIGRALMLPVAILPAAGILLAIGNAMQNKDMIQVLHFLSNDNVQLVAGVM 60 Query: 61 MNSGQIVFDNLSLLFXXXXXXXXXXXXXXXXXXXXXXFLXXXXXXXXXXXX--------- 111 ++GQIVFDNL LLF +L Sbjct: 61 ESAGQIVFDNLPLLFAVGVAIGLANGDGVAGIAAIIGYLVMNVSMSAVLLANGTIPSDSV 120 Query: 112 -XAYVLSWKDYAYSSVLGIPTLQTGVFGGILVGILASAMYKRFFRIELPSYLGFFAGKRF 170 A + AY ++LGIPTL TGVFGGI+VG+LA+ ++ RF+ IELP YLGFFAGKRF Sbjct: 121 ERAKFFTENHPAYVNMLGIPTLATGVFGGIIVGVLAALLFNRFYTIELPQYLGFFAGKRF 180 Query: 171 VPIMTAVTSXXXXXXXXXIWPPIQHGLNYVSQSMINTNLTLSAFIFGVIERSLIPFGLHH 230 VPI+T++++ IWPPIQHGLN S ++ N TL+AFIFGVIERSLIPFGLHH Sbjct: 181 VPIVTSISALILGLIMLVIWPPIQHGLNAFSTGLVEANPTLAAFIFGVIERSLIPFGLHH 240 Query: 231 IFYSPFWYEFGSYVDKAGDLIRGDQRIFMQQLRDGVEFTAGTFTTGKYPFMMFGLPAAAL 290 IFYSPFWYEF SY AG++IRGDQRIFM Q++DGV+ TAGTF TGKYPFMMFGLPAAAL Sbjct: 241 IFYSPFWYEFFSYKSAAGEIIRGDQRIFMAQIKDGVQLTAGTFMTGKYPFMMFGLPAAAL 300 Query: 291 AIYHEARPENKRVVGSLMISAALTSFLTGITEPLEFSFLFVAPLLFAVHAVFAGLSFMTM 350 AIYHEA+P+NK++V +M SAALTSFLTGITEPLEFSFLFVAP+LFA+H +FAGLSFM M Sbjct: 301 AIYHEAKPQNKKLVAGIMGSAALTSFLTGITEPLEFSFLFVAPVLFAIHCLFAGLSFMVM 360 Query: 351 HILNVKIGMTFSGGFIDYMLFGVIPNRTAWWLVIPVGLVLAVIYYFGFRFVIRKFNLRTP 410 +LNVKIGMTFSGG IDY LFG++PNRTAWWLVIPVGL LAVIYYFGFRF IRKFNL+TP Sbjct: 361 QLLNVKIGMTFSGGLIDYFLFGILPNRTAWWLVIPVGLGLAVIYYFGFRFAIRKFNLKTP 420 Query: 411 GREDPSDD 418 GRED +++ Sbjct: 421 GREDAAEE 428 >BH0844_1 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 424 Score = 506 bits (1304), Expect = e-143, Method: Compositional matrix adjust. Identities = 253/418 (60%), Positives = 299/418 (71%), Gaps = 1/418 (0%) Query: 1 MFKKLFGVLQRVGKALMXXXXXXXXXXXXXXXXNMLVNPDFLQYVPALENDVVQAVATVL 60 MFKK FGVLQRVGKALM + L NPD + P L D + +A ++ Sbjct: 1 MFKKSFGVLQRVGKALMLPVALLPAAGILLAFGDALQNPDMTERFPFLTADWIVIIAELM 60 Query: 61 MNSGQIVFDNLSLLFXXXXXXXXXXXXXXXXXXXXXXFLXXXXXXXXXXXXXAYVLSWKD 120 N+G IVF NL+LLF +L ++ Sbjct: 61 QNAGDIVFANLALLFAVGVAIGLSNGDGVAGLAALIGYLIINVTMSVIGHVTPEMVG-DS 119 Query: 121 YAYSSVLGIPTLQTGVFGGILVGILASAMYKRFFRIELPSYLGFFAGKRFVPIMTAVTSX 180 AY+SVLGIPTLQTGVFGGI+ G+L + MYK++F IELPSYLGFFAGKRFVPI TA S Sbjct: 120 PAYASVLGIPTLQTGVFGGIIAGLLGAYMYKKYFNIELPSYLGFFAGKRFVPIATAFFSL 179 Query: 181 XXXXXXXXIWPPIQHGLNYVSQSMINTNLTLSAFIFGVIERSLIPFGLHHIFYSPFWYEF 240 IWP +Q GLN S M +N T++AFIFGVIER+LIPFGLHHIFYSPFW+EF Sbjct: 180 FVGVLLHFIWPTVQDGLNAFSYYMTESNRTVAAFIFGVIERALIPFGLHHIFYSPFWFEF 239 Query: 241 GSYVDKAGDLIRGDQRIFMQQLRDGVEFTAGTFTTGKYPFMMFGLPAAALAIYHEARPEN 300 G Y + AG+++RGDQRIF Q+RDG E TAGTF GK+PFMMFGLPAAALAIYH ARPEN Sbjct: 240 GQYTNAAGEIVRGDQRIFFAQIRDGAELTAGTFMAGKFPFMMFGLPAAALAIYHCARPEN 299 Query: 301 KRVVGSLMISAALTSFLTGITEPLEFSFLFVAPLLFAVHAVFAGLSFMTMHILNVKIGMT 360 K+VV +M SAALTSFLTGITEP+EFSFLFVAPLLFAVH +FAGLSFM MHIL++KIGMT Sbjct: 300 KKVVAGIMASAALTSFLTGITEPIEFSFLFVAPLLFAVHTIFAGLSFMIMHILDIKIGMT 359 Query: 361 FSGGFIDYMLFGVIPNRTAWWLVIPVGLVLAVIYYFGFRFVIRKFNLRTPGREDPSDD 418 FSGG ID++L+GV+PNRTAWWLVIP+GLV +VIYYFGFRF I+KFNL TPGRE+P+++ Sbjct: 360 FSGGVIDFILYGVLPNRTAWWLVIPIGLVFSVIYYFGFRFAIKKFNLMTPGREEPTEE 417 >SA0183_1 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 428 Score = 446 bits (1148), Expect = e-125, Method: Compositional matrix adjust. Identities = 226/435 (51%), Positives = 288/435 (66%), Gaps = 7/435 (1%) Query: 1 MFKKLFGVLQRVGKALMXXXXXXXXXXXXXXXXNMLVNPDFLQYVPALENDVVQAVATVL 60 M KKLFG LQR+GKALM + Y+P ++N VQ VA ++ Sbjct: 1 MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAIQGEALQHYLPFIQNGGVQNVAKLM 60 Query: 61 MNSGQIVFDNLSLLFXXXXXXXXXXXXXXXXXXXXXXFLXXXXXXXXXXXXXAYVLSWKD 120 +G I+F+NL ++F ++ ++ Sbjct: 61 TAAGSIIFENLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMNKTMGDFLQVTPKNVTDPA 120 Query: 121 YAYSSVLGIPTLQTGVFGGILVGILASAMYKRFFRIELPSYLGFFAGKRFVPIMTAVTSX 180 Y+S+LGIPTLQTGVFGGI++G LA+ Y +F+ I LPSYLGFFAGKRFVPIM A TS Sbjct: 121 SGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPSYLGFFAGKRFVPIMMATTSF 180 Query: 181 XXXXXXXXIWPPIQHGLNYVSQSMINTNLTLSAFIFGVIERSLIPFGLHHIFYSPFWYEF 240 IWP IQ GLN S ++++N ++ F+FG I+R LIPFGLHHIF++PFW+EF Sbjct: 181 ILAFPMALIWPTIQSGLNAFSTGLLDSNTGVAVFLFGFIKRLLIPFGLHHIFHAPFWFEF 240 Query: 241 GSYVDKAGDLIRGDQRIFMQQLRDGVEFTAGTFTTGKYPFMMFGLPAAALAIYHEARPEN 300 GS+ + AG++I GDQRIF++Q+R+G TAG F G++P MMFGLPAAALAIYH A+PEN Sbjct: 241 GSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQGEFPVMMFGLPAAALAIYHTAKPEN 300 Query: 301 KRVVGSLMISAALTSFLTGITEPLEFSFLFVAPLLFAVHAVFAGLSFMTMHILNVKIGMT 360 K+VV LM SAALTSFLTGITEPLEFSFLFVAPLLF +HAV GLSF+T+++L+V +G T Sbjct: 301 KKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPLLFFIHAVLDGLSFLTLYLLDVHLGYT 360 Query: 361 FSGGFIDYMLFGVIPNRTAWWLVIPVGLVLAVIYYFGFRFVIRKFNLRTPGREDPSDDVE 420 FSGGFIDY+L GV+PN+T WWLVIPVGLV AVIYYF FRF+I K +TPGRED Sbjct: 361 FSGGFIDYVLLGVLPNKTQWWLVIPVGLVYAVIYYFVFRFLIVKLKYKTPGRED------ 414 Query: 421 ETDTENVSRSGDDLP 435 ++ V+ S +LP Sbjct: 415 -KQSQAVTASATELP 428 >STM2758_1 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 430 Score = 337 bits (863), Expect = 5e-92, Method: Compositional matrix adjust. Identities = 186/429 (43%), Positives = 255/429 (59%), Gaps = 6/429 (1%) Query: 4 KLFGVLQRVGKALMXXXXXXXXXXXXXXXXNMLVNPDFLQYVPALENDVVQAVATVLMNS 63 L +LQ +G++LM +P+ + +P L D + V ++ + Sbjct: 5 NLLQMLQSIGRSLMIPIAMLPAAGILLAFGVSFQDPNIVASLPFLGTDGLVHVLKLMAEA 64 Query: 64 GQIVFDNLSLLFXXXXXXXXXXXXXXXXXXXXXXFLXXXXXXXXXXXXXAYVLSWKDYAY 123 G +F NL LLF FL ++ + Y Sbjct: 65 GSAIFANLPLLFAVGVAVGLSDDQGIAGLSAIAGFLIMNVTIGQFLGITPESVA-QVRDY 123 Query: 124 SSVLGIPTLQTGVFGGILVGILASAMYKRFFRIELPSYLGFFAGKRFVPIMTAVTSXXXX 183 + VLGIP+LQTGVFGGI++GI+A+ +YKR++RI+LPS+L FF+GKRFVPI+T+ + Sbjct: 124 TMVLGIPSLQTGVFGGIIIGIIAAWLYKRYYRIQLPSWLEFFSGKRFVPIVTSFAALFVG 183 Query: 184 XXXXXIWPPIQHGLNYVSQSMINTNLTLSAFIFGVIERSLIPFGLHHIFYSPFWYEFGSY 243 +WPP+QH +N +S +M +SAF+FG +ER LIPFGL+H+++ FW +FG Y Sbjct: 184 LVMAVVWPPVQHLINGLSNTMTVQGPGVSAFLFGFVERLLIPFGLNHVWWPTFWLQFGEY 243 Query: 244 VDKAGDLIRGDQRIFMQQLRDGVEFTAGTFTTGKYPFMMFGLPAAALAIYHEARPENKRV 303 V+KAG ++ GDQ IF QL+D V TAGTF G P MF +PA ALAIY A PEN Sbjct: 244 VNKAGQVVHGDQLIFFAQLKDQVPITAGTFMAGLTPIKMFCIPAIALAIYRCASPENIAR 303 Query: 304 VGSLMISAALTSFLTGITEPLEFSFLFVAPLLFAVHAVFAGLSFMTMHILNVKIGMTFSG 363 V +M+S A+TS + GITEP+EFSFLFVAP+L+ +HAV AGL F+ M +V IG++FSG Sbjct: 304 VKGIMLSGAITSIVCGITEPIEFSFLFVAPVLYGIHAVLAGLVFLLMEWFSVHIGLSFSG 363 Query: 364 GFIDYMLFGVIPNRTAWWLVIPVGLVLAVIYYFGFRFVIRKFNLRTPGREDPSDDVEETD 423 G IDY+ FGV+P W++V PVGLV+ +YY F F IR++NL TPGRE VEET Sbjct: 364 GLIDYLFFGVLPRAPHWYMVFPVGLVMGAVYYVLFTFAIRRWNLLTPGRE-----VEETA 418 Query: 424 TENVSRSGD 432 S D Sbjct: 419 VAQESEQND 427 >SA0233_1 [G] COG1263 Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific Length = 437 Score = 313 bits (803), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 182/433 (42%), Positives = 249/433 (57%), Gaps = 13/433 (3%) Query: 3 KKLFGVLQRVGKALMXXXXXXXXXXXXXXXXNMLVNPDFLQYVPALENDVVQAVATVLMN 62 K LF Q+ GK+ M L NP+ ++ P L+ ++Q + T++ Sbjct: 2 KSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAYPILDITLLQNIFTLMSA 61 Query: 63 SGQIVFDNLSLLFXXXXXXXXXXXXXXXXXXXXXXFLXXXXXXXXXXXXXAYVLSWKDYA 122 +G IVF NL ++F L+ A Sbjct: 62 AGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMNATMNGLLTITGTLAKDQLA 121 Query: 123 YSS---VLGIPTLQTGVFGGILVGILASAMYKRFFRIELPSYLGFFAGKRFVPIMTAVTS 179 + VLGI T++TGVFGGI+ GI+ + ++ ++ ++ LP YLGFF G RFVPI+TA + Sbjct: 122 QNGQGMVLGIQTVETGVFGGIITGIMTAILHNKYHKVVLPPYLGFFGGSRFVPIVTAFAA 181 Query: 180 XXXXXXXXXIWPPIQHGLNYVSQSMINTNLTLSAFIFGVIERSLIPFGLHHIFYSPFWYE 239 IWP IQ G+ +V + T + F++G I R L P GLHHIFY PFW Sbjct: 182 IFLGVLMFFIWPSIQAGIYHVGGFVTKTG-AIGTFVYGFILRLLGPLGLHHIFYLPFWQT 240 Query: 240 -FGSYVDKAGDLIRGDQRIFMQQLRDG--VEFTAGT--FTTGKYPFMMFGLPAAALAIYH 294 G ++ G L++G Q IF QL D ++ +G F +G++ MMFGL AALAIYH Sbjct: 241 ALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGVSRFMSGRFITMMFGLCGAALAIYH 300 Query: 295 EARPENKRVVGSLMISAALTSFLTGITEPLEFSFLFVAPLLFAVHAVFAGLSFMTMHILN 354 A+PE+K+VVG LM+SAALTSFLTGITEPLEFSFLFVAP+L+ +HA F GL+FM I N Sbjct: 301 TAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSFLFVAPILYVIHAFFDGLAFMMADIFN 360 Query: 355 VKIGMTFSGGFIDYMLFGVIP--NRTAWWLVIPVGLVLAVIYYFGFRFVIRKFNLRTPGR 412 + IG TFSGGFID++LFGV+ ++T + VIP+G+V +YY FRF+I KFN +TPGR Sbjct: 361 ITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVIPIGIVWFCLYYIVFRFLITKFNFKTPGR 420 Query: 413 ED--PSDDVEETD 423 ED + VE T+ Sbjct: 421 EDKAAAQQVEATE 433 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.141 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 46,272,846 Number of extensions: 1939633 Number of successful extensions: 4902 Number of sequences better than 1.0e-05: 150 Number of HSP's gapped: 4753 Number of HSP's successfully gapped: 156 Length of query: 687 Length of database: 38,378,431 Length adjustment: 113 Effective length of query: 574 Effective length of database: 25,345,689 Effective search space: 14548425486 Effective search space used: 14548425486 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 119 (50.4 bits)