BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0100.p (544 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0588_4 [T] COG2199 FOG: GGDEF domain 117 6e-26 VC0658_2 [T] COG2199 FOG: GGDEF domain 115 2e-25 CC0091_2 [T] COG2199 FOG: GGDEF domain 114 4e-25 CC0655_2 [T] COG2199 FOG: GGDEF domain 114 5e-25 all1219_3 [T] COG2199 FOG: GGDEF domain 113 1e-24 >RSc0588_4 [T] COG2199 FOG: GGDEF domain Length = 182 Score = 117 bits (292), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 61/171 (35%), Positives = 103/171 (60%), Gaps = 1/171 (0%) Query: 362 EERLHKLAFYDHLTAIPNRAHFKMYMENLISQPEERKQEIALALLDCDRFKQLNDTLGHL 421 E++L LA D LT + NRA+F M+ ++ + ++ A+ LD D FK++NDT GH Sbjct: 5 EQQLKFLAQRDALTGLYNRAYFLQRMQAVLDEAVTDGRQAAVLFLDLDGFKKVNDTAGHE 64 Query: 422 AGDEFLQLLSRELQQTVKGTGQAFRIGGDEFAVVLHRFASPEMLDEILDRLLQLFNKSWS 481 AGD L+++++ L V+ T R+GGDEF V+L + S +++ R++ + +S Sbjct: 65 AGDHLLRIVAQRLSACVRQTDTLARLGGDEFVVLLDQVRSLAEAEQLARRIIAAIAQPFS 124 Query: 482 VNKGSSFHTSASIGVALYPQHGSSINELLRAADLAMYRSKKHGGNEANLYS 532 G+ ++ ASIG+A++P HG LLR AD AMY +K++G N+ +++ Sbjct: 125 TG-GTEYYLGASIGIAVHPDHGQDAATLLRCADAAMYNAKQNGRNQHRVFT 174 >VC0658_2 [T] COG2199 FOG: GGDEF domain Length = 219 Score = 115 bits (287), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 60/170 (35%), Positives = 100/170 (58%), Gaps = 2/170 (1%) Query: 362 EERLHKLAFYDHLTAIPNRAHFKMYMENLISQPEERKQEIALALLDCDRFKQLNDTLGHL 421 E +L++LA+YD LT + NR+ + + I + L LD DRFK +ND+LGH Sbjct: 52 ESKLNQLAYYDSLTGLANRSLLLQKLSDAIETAQNSHSTGVLFYLDLDRFKTINDSLGHT 111 Query: 422 AGDEFLQLLSRELQQTVKGTGQAFRIGGDEFAVVLHRFASPEMLDEILDRLLQLFNKSWS 481 GD+ ++ ++ ++ K + RIGGDEFAV++ + SP +E+ +LL L + ++ Sbjct: 112 IGDQLIKAVALRMEAWTKNDYLSARIGGDEFAVLIP-YLSPAKAEEVAKQLLTLISNPYA 170 Query: 482 VNKGSSFHTSASIGVALYPQHGSSINELLRAADLAMYRSKKHGGNEANLY 531 V+ F+ + SIG++++P GS ++LR AD A+YR+K G N+ Y Sbjct: 171 VDD-HQFYCTVSIGISVFPSVGSCNIDVLRQADTALYRAKASGRNKFMFY 219 >CC0091_2 [T] COG2199 FOG: GGDEF domain Length = 254 Score = 114 bits (285), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 69/214 (32%), Positives = 109/214 (50%), Gaps = 19/214 (8%) Query: 329 VWLETKVTPIKGGGAVPAQF-----------------MLVSREITERKQHEERLHKLAFY 371 V E ++ P+ GG +P + +L R++ ER+ EE++ LA + Sbjct: 27 VRREGRLRPLVGGREIPVEVFSRLMDDGARVETSGLTVLAVRDLRERRAAEEKIRYLAEH 86 Query: 372 DHLTAIPNRAHFKMYMENLISQPEERKQEIALALLDCDRFKQLNDTLGHLAGDEFLQLLS 431 D LT + NR +M + I + E + +A+ +D D FK+ ND GHLAGD L + Sbjct: 87 DGLTGLLNRNSLQMRLAAAIDRVEASGESLAVICIDLDHFKEANDQHGHLAGDALLVETA 146 Query: 432 RELQQTVKGTGQAFRIGGDEFAVV-LHRFASPEMLDEILDRLLQLFNKSWSVNKGSSFHT 490 R LQ V+ A R+GGDEF VV + P + E+ RL+++ + G Sbjct: 147 RRLQSAVQAPSFAARLGGDEFIVVQIAGGDQPAVAAELAGRLIEMLAAPVPFD-GQELAM 205 Query: 491 SASIGVALYPQHGSSINELLRAADLAMYRSKKHG 524 +S+GV+LYP G + L+ AD+A+YR+K+ G Sbjct: 206 GSSLGVSLYPDDGRTAEALMANADMALYRAKESG 239 >CC0655_2 [T] COG2199 FOG: GGDEF domain Length = 205 Score = 114 bits (284), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 63/179 (35%), Positives = 99/179 (55%), Gaps = 1/179 (0%) Query: 353 REITERKQHEERLHKLAFYDHLTAIPNRAHFKMYMENLISQPEERKQEIALALLDCDRFK 412 ++IT+R++ E + LAF D LT +PNR F+ + +S E + L ++D D FK Sbjct: 3 KDITDRRRQTEAIETLAFKDPLTGLPNRTLFQHRFQEAVSASETLGEMFGLIMIDVDHFK 62 Query: 413 QLNDTLGHLAGDEFLQLLSRELQQTVKGTGQAFRIGGDEFAVVLHRFASPEMLDEILDRL 472 +NDTLGH AGD L+ L+ LQ + R+GGDEFAV+L + ++ L Sbjct: 63 DINDTLGHDAGDALLKRLAGMLQHAFRTGDTVARLGGDEFAVILRGLHGEADMTRPIEAL 122 Query: 473 LQLFNKSWSVNKGSSFHTSASIGVALYPQHGSSINELLRAADLAMYRSKKHGGNEANLY 531 L + + G SF SASIG AL+ + +++ AD+A+YR+K+ G N + ++ Sbjct: 123 QDLLRRPIQ-HGGRSFTASASIGAALHGDLDADPAHMIKNADIALYRAKEAGRNRSIVF 180 >all1219_3 [T] COG2199 FOG: GGDEF domain Length = 199 Score = 113 bits (282), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 61/187 (32%), Positives = 106/187 (56%), Gaps = 2/187 (1%) Query: 353 REITERKQHEERLHKLAFYDHLTAIPNRAHFKMYMENLISQPEERKQEIALALLDCDRFK 412 R E K E+ L LA++D LT +PNR +F + I+ ++ + L +D D FK Sbjct: 15 RSNNELKLKEDHLRYLAYHDALTGLPNRKYFAEQLYESINWAKDNNLSLGLLFIDLDGFK 74 Query: 413 QLNDTLGHLAGDEFLQLLSRELQQTVKGTGQAFRIGGDEFAVVLHRFASPEMLDEILDRL 472 Q+ND+LGH GD L ++++ L +++ + R+GGDEF V+L + ++ ++ +++ Sbjct: 75 QVNDSLGHEMGDRLLVVIAQRLSNSLRASDTVARLGGDEFTVILQAIPNVQVAAKVAEKI 134 Query: 473 LQLFNKSWSVNKGSSFHTSASIGVALYPQHGSSINELLRAADLAMYRSKKHGGNEANLYS 532 L + +N G + S SIG+++YP+ + LL+ AD AMYR+K G N L + Sbjct: 135 LANITEPIVLN-GCTAKVSVSIGISIYPETSLDPDTLLKKADAAMYRAKHLGKNRFEL-A 192 Query: 533 ELVDEEY 539 + V+ E+ Sbjct: 193 DRVNTEF 199 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.133 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 37,101,551 Number of extensions: 1421891 Number of successful extensions: 2909 Number of sequences better than 1.0e-05: 138 Number of HSP's gapped: 2767 Number of HSP's successfully gapped: 138 Length of query: 544 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 433 Effective length of database: 25,576,357 Effective search space: 11074562581 Effective search space used: 11074562581 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)