BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0087.p (238 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yufM [KT] COG4565 Response regulator of citrate/malate metabo... 229 4e-60 BH0398 [KT] COG4565 Response regulator of citrate/malate metabolism 222 3e-58 dcuR [KT] COG4565 Response regulator of citrate/malate metabolism 198 8e-51 ZyjdG [KT] COG4565 Response regulator of citrate/malate metabolism 198 8e-51 ECs5106 [KT] COG4565 Response regulator of citrate/malate metabo... 198 8e-51 >BS_yufM [KT] COG4565 Response regulator of citrate/malate metabolism Length = 235 Score = 229 bits (583), Expect = 4e-60, Method: Compositional matrix adjust. Identities = 116/237 (48%), Positives = 158/237 (66%), Gaps = 4/237 (1%) Query: 3 IKVLIVEDDPMVAKFNRHYVEQVQGFEFAGWAASVDEARKLLAGLKVDLILLDIYMPRTS 62 I VLIVEDDPMV + N+ Y+ Q+ GF+ G A+S A +L +DLILLDIYMP + Sbjct: 2 INVLIVEDDPMVGELNKRYLSQIDGFQLKGIASSFQSALHILGEHHIDLILLDIYMPGKN 61 Query: 63 GLQLLSSLREQGSVVDIIVISAASDNASIRKALQLGAVDYLIKPFEFARFQAALSAYQED 122 GL+LL+ LR Q VD+IVISAAS+ I+K L+ GAVDYLIKPFEF RFQ ALS Y+ Sbjct: 62 GLELLTELRAQNEAVDVIVISAASELDVIKKTLRYGAVDYLIKPFEFERFQTALSDYRRK 121 Query: 123 YMLMKHREHLSQEQLD-KLLRHSTGTEEDKSGALPKGLTEGTLESIWSTIQKLESPVFST 181 + ++SQ++LD +L + TE+ + LPKGLT+ TL+ IWS+IQ E+ F+T Sbjct: 122 QKVYSTHRNMSQKELDAELFQKKEATEKVQ---LPKGLTKSTLKLIWSSIQSFENESFTT 178 Query: 182 EEITAEAPISRISVRKYLTFLTEAGVLEMEISYGAVGRPVYMYTVTASGHEIIAAYI 238 E++ IS++S+RKYL FL + VL +E++YG +GRPV+ Y V S I Y+ Sbjct: 179 EDLAKHTEISQVSIRKYLKFLEDIQVLNVEMAYGTIGRPVFQYNVNNSNINGIKQYL 235 >BH0398 [KT] COG4565 Response regulator of citrate/malate metabolism Length = 237 Score = 222 bits (566), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 115/236 (48%), Positives = 161/236 (68%), Gaps = 2/236 (0%) Query: 3 IKVLIVEDDPMVAKFNRHYVEQVQGFEFAGWAASVDEARKLLAGLKVDLILLDIYMPRTS 62 I VLIVEDDPMVA+FN+ YV+ V GF G A V+EA+ ++ KVDLILLD+YMP + Sbjct: 2 IHVLIVEDDPMVAEFNKRYVKNVDGFHLVGIAYHVEEAKTVIEEKKVDLILLDVYMPGEN 61 Query: 63 GLQLLSSLREQGSVVDIIVISAASDNASIRKALQLGAVDYLIKPFEFARFQAALSAYQED 122 GL+LLS +R + +D+I+I+AASD I+ AL+ GAVDYLIKPFEF RF+ AL +Y++ Sbjct: 62 GLELLSYIRAEKLGIDVILITAASDADHIQTALRYGAVDYLIKPFEFERFEQALLSYKDK 121 Query: 123 YMLMKHREHLSQEQLDKLLRHSTGTEEDKSGALPKGLTEGTLESIWSTIQKLESPVFSTE 182 ++ +E L+QE+LD+LL S + D+ ALPKGLT+ TL+ I I+K+ FST+ Sbjct: 122 QYALREKEQLNQEELDQLLL-SDDKDPDQEIALPKGLTQSTLQIIVEEIRKIGETPFSTD 180 Query: 183 EITAEAPISRISVRKYLTFLTEAGVLEMEISYGAVGRPVYMYTVTASGHEIIAAYI 238 E+ + ISR+S+RKYL FL + LE ++YG +GRPVY Y + + Y+ Sbjct: 181 ELAEQTEISRVSIRKYLKFLVDIECLEERLTYG-IGRPVYSYQYIKENGDRLKQYL 235 >dcuR [KT] COG4565 Response regulator of citrate/malate metabolism Length = 239 Score = 198 bits (503), Expect = 8e-51, Method: Compositional matrix adjust. Identities = 99/238 (41%), Positives = 151/238 (63%), Gaps = 3/238 (1%) Query: 3 IKVLIVEDDPMVAKFNRHYVEQVQGFEFAGWAASVDEARKLL--AGLKVDLILLDIYMPR 60 I VLI++DD MVA+ NR YV Q+ GF+ G A+++++A++++ + +DLILLDIYM + Sbjct: 2 INVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIFNSDTPIDLILLDIYMQK 61 Query: 61 TSGLQLLSSLREQGSVVDIIVISAASDNASIRKALQLGAVDYLIKPFEFARFQAALSAYQ 120 +GL LL L D+IVIS+A+D A+I+ +L G VDYLIKPF+ +RF+ AL+ ++ Sbjct: 62 ENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVDYLIKPFQASRFEEALTGWR 121 Query: 121 EDYMLMKHREHLSQEQLDKLLRHSTGTEEDKSGALPKGLTEGTLESIWSTIQKLESPVFS 180 + M ++ ++ Q +LD+L+ S+ E+D LPKGLT TL ++ I + FS Sbjct: 122 QKKMALEKHQYYDQAELDQLIHGSSSNEQDPR-RLPKGLTPQTLRTLCQWIDAHQDYEFS 180 Query: 181 TEEITAEAPISRISVRKYLTFLTEAGVLEMEISYGAVGRPVYMYTVTASGHEIIAAYI 238 T+E+ E ISR+S RKYL +L +L I YG GRPVY Y + A + ++ Y Sbjct: 181 TDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTGRPVYRYRIQAEHYSLLKQYC 238 >ZyjdG [KT] COG4565 Response regulator of citrate/malate metabolism Length = 239 Score = 198 bits (503), Expect = 8e-51, Method: Compositional matrix adjust. Identities = 99/238 (41%), Positives = 151/238 (63%), Gaps = 3/238 (1%) Query: 3 IKVLIVEDDPMVAKFNRHYVEQVQGFEFAGWAASVDEARKLL--AGLKVDLILLDIYMPR 60 I VLI++DD MVA+ NR YV Q+ GF+ G A+++++A++++ + +DLILLDIYM + Sbjct: 2 INVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIFNSDTPIDLILLDIYMQK 61 Query: 61 TSGLQLLSSLREQGSVVDIIVISAASDNASIRKALQLGAVDYLIKPFEFARFQAALSAYQ 120 +GL LL L D+IVIS+A+D A+I+ +L G VDYLIKPF+ +RF+ AL+ ++ Sbjct: 62 ENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVDYLIKPFQASRFEEALTGWR 121 Query: 121 EDYMLMKHREHLSQEQLDKLLRHSTGTEEDKSGALPKGLTEGTLESIWSTIQKLESPVFS 180 + M ++ ++ Q +LD+L+ S+ E+D LPKGLT TL ++ I + FS Sbjct: 122 QKKMALEKHQYYDQAELDQLIHGSSSNEQDPR-RLPKGLTPQTLRTLCQWIDAHQDYEFS 180 Query: 181 TEEITAEAPISRISVRKYLTFLTEAGVLEMEISYGAVGRPVYMYTVTASGHEIIAAYI 238 T+E+ E ISR+S RKYL +L +L I YG GRPVY Y + A + ++ Y Sbjct: 181 TDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTGRPVYRYRIQAEHYSLLKQYC 238 >ECs5106 [KT] COG4565 Response regulator of citrate/malate metabolism Length = 239 Score = 198 bits (503), Expect = 8e-51, Method: Compositional matrix adjust. Identities = 99/238 (41%), Positives = 151/238 (63%), Gaps = 3/238 (1%) Query: 3 IKVLIVEDDPMVAKFNRHYVEQVQGFEFAGWAASVDEARKLL--AGLKVDLILLDIYMPR 60 I VLI++DD MVA+ NR YV Q+ GF+ G A+++++A++++ + +DLILLDIYM + Sbjct: 2 INVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIFNSDTPIDLILLDIYMQK 61 Query: 61 TSGLQLLSSLREQGSVVDIIVISAASDNASIRKALQLGAVDYLIKPFEFARFQAALSAYQ 120 +GL LL L D+IVIS+A+D A+I+ +L G VDYLIKPF+ +RF+ AL+ ++ Sbjct: 62 ENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVDYLIKPFQASRFEEALTGWR 121 Query: 121 EDYMLMKHREHLSQEQLDKLLRHSTGTEEDKSGALPKGLTEGTLESIWSTIQKLESPVFS 180 + M ++ ++ Q +LD+L+ S+ E+D LPKGLT TL ++ I + FS Sbjct: 122 QKKMALEKHQYYDQAELDQLIHGSSSNEQDPR-RLPKGLTPQTLRTLCQWIDAHQDYEFS 180 Query: 181 TEEITAEAPISRISVRKYLTFLTEAGVLEMEISYGAVGRPVYMYTVTASGHEIIAAYI 238 T+E+ E ISR+S RKYL +L +L I YG GRPVY Y + A + ++ Y Sbjct: 181 TDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTGRPVYRYRIQAEHYSLLKQYC 238 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.133 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,111,727 Number of extensions: 582342 Number of successful extensions: 3004 Number of sequences better than 1.0e-05: 350 Number of HSP's gapped: 2993 Number of HSP's successfully gapped: 350 Length of query: 238 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 135 Effective length of database: 26,499,029 Effective search space: 3577368915 Effective search space used: 3577368915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)