BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0081.p (166 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3351 [K] COG1522 Transcriptional regulators 173 1e-43 BS_yugG [K] COG1522 Transcriptional regulators 149 2e-36 MA0924 [K] COG1522 Transcriptional regulators 138 4e-33 PH0140 [K] COG1522 Transcriptional regulators 56 2e-08 PAB1236 [K] COG1522 Transcriptional regulators 53 2e-07 >BH3351 [K] COG1522 Transcriptional regulators Length = 164 Score = 173 bits (439), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 79/150 (52%), Positives = 116/150 (77%), Gaps = 1/150 (0%) Query: 18 DARSSTALMATLLGAEEEDVKQAIEEMERDHVIVKYATVVNWDKVSD-ERVTALIEVQIT 76 + R +A ++ A EE+V I+++E DHVI+ Y+ V++W KV + E VTA+I+V++T Sbjct: 15 NGRVPIPTLAKMIDATEEEVTAIIKKLENDHVILSYSAVIDWSKVKEVETVTAMIDVKVT 74 Query: 77 PERGRGFEGIAERIYLYPQVKSVYLMSGAYDLLVEVEGRNLREVANFVSEKLSPIDAVLS 136 P+RG GF+ +AERIY +P+VK++YLMSGAYDL V +EG+ + EVA FVSEKLS ID VLS Sbjct: 75 PQRGVGFDEVAERIYRFPEVKALYLMSGAYDLSVVIEGKTMSEVARFVSEKLSTIDTVLS 134 Query: 137 TKTNFTLKKYKQDGIIFEEHEEDNRLMISP 166 T T+F LKKYK DG++F++ E+D R++++P Sbjct: 135 TTTHFQLKKYKHDGVVFKKDEDDKRIVVTP 164 >BS_yugG [K] COG1522 Transcriptional regulators Length = 166 Score = 149 bits (377), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 70/150 (46%), Positives = 111/150 (74%), Gaps = 1/150 (0%) Query: 18 DARSSTALMATLLGAEEEDVKQAIEEMERDHVIVKYATVVNWDKV-SDERVTALIEVQIT 76 ++R+ +A + G +VK I+++E++ VI+ Y+ +++W KV E VTA+I+V++T Sbjct: 17 NSRADLETIAKMAGIPVNEVKTIIDKLEKEKVIIDYSAMIDWRKVDGHEGVTAMIDVKVT 76 Query: 77 PERGRGFEGIAERIYLYPQVKSVYLMSGAYDLLVEVEGRNLREVANFVSEKLSPIDAVLS 136 P+RG GF+ +AERIY + +V+SVYLMSG YDL V + G ++ +VA FVS+KLS +D+V+S Sbjct: 77 PKRGVGFDEVAERIYRFQEVESVYLMSGVYDLSVVIRGNSMSDVARFVSDKLSTLDSVVS 136 Query: 137 TKTNFTLKKYKQDGIIFEEHEEDNRLMISP 166 T T+F LKKYK DG +FE ++D R+++SP Sbjct: 137 TTTHFILKKYKHDGKVFETGDDDKRIVVSP 166 >MA0924 [K] COG1522 Transcriptional regulators Length = 163 Score = 138 bits (348), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 66/148 (44%), Positives = 103/148 (69%) Query: 18 DARSSTALMATLLGAEEEDVKQAIEEMERDHVIVKYATVVNWDKVSDERVTALIEVQITP 77 DAR+S +A L G +++ Q I ++E VI Y T+V+WD + + V A+IE+++T Sbjct: 15 DARASPEEIAALTGMSAQEISQTIAKLEETGVIRHYKTIVDWDLIGENYVYAVIELKVTL 74 Query: 78 ERGRGFEGIAERIYLYPQVKSVYLMSGAYDLLVEVEGRNLREVANFVSEKLSPIDAVLST 137 ER +G++ IAERIY +P+V+SV L+SG YD+ + V G+++++VA FV+EK++ +D V ST Sbjct: 75 ERNQGYQAIAERIYKFPEVRSVRLLSGDYDISLTVRGKSMKDVAFFVAEKIATLDQVQST 134 Query: 138 KTNFTLKKYKQDGIIFEEHEEDNRLMIS 165 T+F LK YK+DG+I E E RL +S Sbjct: 135 STHFVLKTYKEDGVILHEPESIKRLPVS 162 >PH0140 [K] COG1522 Transcriptional regulators Length = 158 Score = 56.2 bits (134), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 35/131 (26%), Positives = 75/131 (57%), Gaps = 6/131 (4%) Query: 18 DARSSTALMATLLGAEEEDVKQAIEEMERDHVIVKYATVVNWDKVSDERVTALIEVQITP 77 D R S + ++ + E V+ + + ++ VI K+A ++N K E + A I V P Sbjct: 26 DGRMSYSEISRRINVPESTVRARVNRLVKEGVIRKFAALINPFKAGYE-IVAFIAVDAEP 84 Query: 78 ERGRGFEGIAERIYLYPQVKSVYLMSGAYDLLVEVEGRNLREVANFVSEKLSPIDAVLST 137 + + + E + +P+V + +++GA+D+ ++V ++L+E+ F+ EK++ ID + ST Sbjct: 85 AK---VKQVVEELAKFPEVDVLGIVTGAHDIFMQVTVKDLQELERFILEKMAKIDGIKST 141 Query: 138 KTNF--TLKKY 146 +T+ ++KK+ Sbjct: 142 ETSILTSVKKW 152 >PAB1236 [K] COG1522 Transcriptional regulators Length = 150 Score = 53.1 bits (126), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 37/132 (28%), Positives = 66/132 (50%), Gaps = 1/132 (0%) Query: 18 DARSSTALMATLLGAEEEDVKQAIEEMERDHVIVKYATVVNWDKVSDERVTALIEVQITP 77 +AR + A ++ LG V I+ + + +I ++ N+ K+ + TA I ++ P Sbjct: 19 NARENIATLSKKLGIPRTTVHYRIKRLVEEGIIERFTIKPNYKKL-NLGTTAFILIRYDP 77 Query: 78 ERGRGFEGIAERIYLYPQVKSVYLMSGAYDLLVEVEGRNLREVANFVSEKLSPIDAVLST 137 + G +AE+I P V V++++G +DLL++V N EV V +KL I V T Sbjct: 78 DSGLTQRQVAEQIAKIPGVYEVHIIAGEWDLLLKVRASNAEEVGRIVIDKLREIRGVGQT 137 Query: 138 KTNFTLKKYKQD 149 T + K++ Sbjct: 138 VTMVSFDTVKEE 149 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.132 0.355 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 10,409,538 Number of extensions: 401676 Number of successful extensions: 885 Number of sequences better than 1.0e-05: 25 Number of HSP's gapped: 903 Number of HSP's successfully gapped: 25 Length of query: 166 Length of database: 38,378,431 Length adjustment: 98 Effective length of query: 68 Effective length of database: 27,075,699 Effective search space: 1841147532 Effective search space used: 1841147532 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 112 (47.8 bits)