BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0077.p (436 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SSO2040 [Q] COG1228 Imidazolonepropionase and related amidohydro... 91 5e-18 SSO2527 [Q] COG1228 Imidazolonepropionase and related amidohydro... 86 1e-16 BH2935 [Q] COG1228 Imidazolonepropionase and related amidohydrol... 84 3e-16 CC3125 [Q] COG1228 Imidazolonepropionase and related amidohydrol... 84 6e-16 CC0300 [Q] COG1228 Imidazolonepropionase and related amidohydrol... 77 8e-14 >SSO2040 [Q] COG1228 Imidazolonepropionase and related amidohydrolases Length = 408 Score = 90.5 bits (223), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 114/444 (25%), Positives = 190/444 (42%), Gaps = 75/444 (16%) Query: 15 AITNARIFDGEKVISARHIIIQEGMIIAVGGEIPAHAAVIDADNATLMPGLIDAHVHTSI 74 AI N +F+G+++I +I ++EG I+ V E + IDA + +MPGLIDAH+H S Sbjct: 4 AIRNGIVFNGKEIIGKTNIYVREGKIVEVSREDHSAKMEIDAKDMFVMPGLIDAHIHLS- 62 Query: 75 GGLR--------------DALKFGVTTELEMNGGFT---EKGREIQLQNLGGIAD----- 112 G++ L+ E + GFT + G + L + D Sbjct: 63 -GIKGGSLLKIMFEKPEYRVLRAAKWLEKLLLAGFTTVRDCGETVSLALKRALNDGIIGG 121 Query: 113 --VRSAGMAVTAPGGHPDELLPDDGEIPDFVLKELEKLTEKDRNAMLEAFAHDHDEAPQV 170 V +AG +T GH GE+ V E K +F+ D Sbjct: 122 PKVIAAGKPITQTFGH--------GELSHDVPLEFSKTM---------SFSEFCD----- 159 Query: 171 TTIEEAVKHVHTQVENGADYIKIMIEEGTV--MGVPGLPVLSEDILKAAVREAHKLNKIV 228 +E + + +GAD+IKI G + P P LS + ++A V EA K+N V Sbjct: 160 -GMESCIHAARKVLRDGADFIKIFATGGVLSQRDRPEHPQLSYEEIRAIVNEAEKVNTYV 218 Query: 229 LAHVLTADSSQSAIRIGVDGLAHLFIDRPESTSEVVAAIKDSGAFVTPCLVLNASIIGNP 288 AH ++ AI GV L H + + E+ + +K+ +TP L + +I Sbjct: 219 AAHAHGDRGARIAIEAGVKTLEHGTLLKDET----LKLMKEKNVTLTPTLTIQ-ELIFKY 273 Query: 289 ASELAGDPRVNSKLSQEWIDILNSSFNTYPQGSLDNSFKSVMELHKAGVDILVGTDVSPV 348 ++ D K++ +I N V + + GV I+ GTD+ Sbjct: 274 GKQIGVDEWGLQKITSVRENIAN----------------VVRKAKEYGVTIISGTDLGFE 317 Query: 349 PLHNLGGLAHGASVHHEMQLLVQAGFTPVEALQSATSKPARRFGLQDRGRIAEGLRADLV 408 L + G + + L+ + G +P+EAL+++T A G+ + G I G AD++ Sbjct: 318 T--GLEEIDMGKNWMETVLLVERGGLSPIEALRASTYNAAMAIGI-NAGVIEIGKDADII 374 Query: 409 LVDGDPTTSISDSLSIQAVWLKGE 432 +++GDPT ++ + I V G+ Sbjct: 375 IINGDPTLNVREISKITHVVKNGK 398 >SSO2527 [Q] COG1228 Imidazolonepropionase and related amidohydrolases Length = 407 Score = 85.9 bits (211), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 108/442 (24%), Positives = 191/442 (43%), Gaps = 71/442 (16%) Query: 15 AITNARIFDGEKVISARHIIIQEGMIIAVGGEIPAHAAVIDADNATLMPGLIDAHVH-TS 73 I N +F+G+ V+ +I +++G I+ + E IDA + +MPGLIDAH+H T Sbjct: 4 GIKNGNVFNGKGVVGKVNIYVKDGKIVEISKENKQAKIEIDAKDMFVMPGLIDAHIHLTG 63 Query: 74 IGG---LRD--------ALKFGVTTELEMNGGFT---EKGREIQLQNLGGIAD------- 112 I G L+ L+ E + GFT + G I L G+++ Sbjct: 64 IKGGSLLKTMFEKPEYRVLRASKWLEKLLLAGFTTVRDCGETISLALKRGVSEGIINGPR 123 Query: 113 VRSAGMAVTAPGGHPDELLPDDGEIPDFVLKELEKLTEKDRNAMLEAFAHDHDEAPQVTT 172 + +AG ++ GH GE+ V E +M +F+ D Sbjct: 124 IIAAGKPISQTFGH--------GELSHDVPLEF---------SMALSFSEFCD------G 160 Query: 173 IEEAVKHVHTQVENGADYIKIMIEEGTV--MGVPGLPVLSEDILKAAVREAHKLNKIVLA 230 +E ++ + +GAD+IKI G + P P LS + + A V EA K+N V A Sbjct: 161 VESCIRASRKVLRDGADFIKIFATGGVLSQRDRPEHPQLSYEEISAIVNEAEKINTYVAA 220 Query: 231 HVLTADSSQSAIRIGVDGLAHLFIDRPESTSEVVAAIKDSGAFVTPCLVLNASIIGNPAS 290 H ++ A+ G+ L H + R E+ + +++ +TP L + +I Sbjct: 221 HAHGDKGARIAVEAGIKTLEHGTLLRDET----LKLMRERNVTLTPTLTIQ-ELIYKYGK 275 Query: 291 ELAGDPRVNSKLSQEWIDILNSSFNTYPQGSLDNSFKSVMELHKAGVDILVGTDVSPVPL 350 ++ D K+++ ++N V + + GV I+ GTD+ Sbjct: 276 QIGVDEWGLQKINEVRESVVN----------------VVKKAEEYGVMIITGTDLGFET- 318 Query: 351 HNLGGLAHGASVHHEMQLLVQAGFTPVEALQSATSKPARRFGLQDRGRIAEGLRADLVLV 410 L + G + + L+ + G + + +L+++T A G+ + G I G AD++++ Sbjct: 319 -GLEEIDIGKNWMESVLLVERGGLSTISSLRASTYNSAFAIGI-NAGAIEVGKDADIIVI 376 Query: 411 DGDPTTSISDSLSIQAVWLKGE 432 DGDPT +I D ++ V G+ Sbjct: 377 DGDPTENIRDLSKVRRVVKNGK 398 >BH2935 [Q] COG1228 Imidazolonepropionase and related amidohydrolases Length = 394 Score = 84.3 bits (207), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 109/431 (25%), Positives = 181/431 (41%), Gaps = 63/431 (14%) Query: 15 AITNARIFDG--EKVISARHIIIQEGMIIAVGGEIPAHAAV-IDADNATLMPGLIDAHVH 71 I N R+ G ++VI I+ + I+ V A V ID +++PGLID HVH Sbjct: 4 CIHNTRLIIGIADRVIENGSILFDQDKILKVSAHSITDADVFIDGTTFSVLPGLIDVHVH 63 Query: 72 TSIGGLRDALKFGVTTELEMNGGFTEKGREIQLQNLGGIADVRSAGMAVTAPGGHPDELL 131 + D TE + F + +Q GI VR+ G Sbjct: 64 LGMDCSVDPFDTMENTE-DAFTSFIAYKQGLQFLR-AGITTVRNLGTKNNV--------- 112 Query: 132 PDDGEIPDFVLKELEK--LTEKDR-----NAMLEAFAHDHDEAPQVTTIEEAVKHVHTQV 184 D + K+ + L R ++ H +V +E +K ++ Sbjct: 113 -------DIIYKKAVESGLVIGPRVYAAGQPLIMTGGHGQPMGTEVDGKDEVIKATRQRI 165 Query: 185 ENGADYIKIMIEEGTVM--GVPGLPVLSEDILKAAVREAHKLNKIVLAHVLTADSSQSAI 242 + GAD IKIM G + PG + D LK +EA K + AH + + ++AI Sbjct: 166 KEGADLIKIMATGGVLTKGNHPGAVHFNVDELKCICQEAAKASLTTAAHAIGLNGVKNAI 225 Query: 243 RIGVDGLAH-LFIDRPESTSEVVAAIKDSGAFVTPCLVLNASIIGNPASELAGDPRVNSK 301 + GV+ + H ++D+ E V ++D G ++TP L+ + N + Sbjct: 226 KAGVNTIEHGYYLDK-----ETVDTMRDLGIYLTPTLLAPTLLQKN-----------SEH 269 Query: 302 LSQEWIDILNSSFNTYPQGSLDNSFKSVMELHKAGVDILVGTDVSPVPLHNLGGLAHGAS 361 + Q ID + + + SF+ +E GV I+ GTD P +N G L Sbjct: 270 IPQHMIDKIEEIEKEHRK-----SFRLALE---RGVKIIAGTDAG-TPFNNPGQLV---- 316 Query: 362 VHHEMQLLVQAGFTPVEALQSATSKPARRFGLQDR-GRIAEGLRADLVLVDGDPTTSISD 420 E++++++ G T +A++SAT A+ + G + E ADL++V G+P IS+ Sbjct: 317 --EELKIMLEEGMTTAQAIESATYLGAKCLKVDSLIGSLEEQKLADLIVVKGNPLEDISN 374 Query: 421 SLSIQAVWLKG 431 +IQ V+ G Sbjct: 375 LKNIQHVFKGG 385 >CC3125 [Q] COG1228 Imidazolonepropionase and related amidohydrolases Length = 431 Score = 83.6 bits (205), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 128/458 (27%), Positives = 185/458 (40%), Gaps = 114/458 (24%) Query: 11 AGVTAITNARIFDGE--KVISARHIIIQEGMIIAVG------GEIPAHAAVIDADNATLM 62 A V+ + ++ D + KV++ + + I+ I++VG + P A V D T++ Sbjct: 21 AQVSYVRAGKLVDPQAGKVLTDQILRIEGERIVSVGPWKGAPKDGPKDAKVTDWSGLTVL 80 Query: 63 PGLIDAHVH---------TSIGGLRDALKFGVT----TELEMNGGFTEKGREIQLQNLGG 109 PGLID H H ++ LR A + + GFT R++ G Sbjct: 81 PGLIDMHTHLVDDEQSENIALPLLRSAAQQAYIGAGHARTTLMAGFTSV-RDVGTWRALG 139 Query: 110 IADVRSA--------------GMAVTAPGG-------HPDELLPDDGEIPDFVLKELEKL 148 A +R A G VTAPGG PD +P D Sbjct: 140 DAALRDAITEGLVPGPRMSVAGAYVTAPGGGGEITGVAPDVTIPAD-------------- 185 Query: 149 TEKDRNAMLEAFAHDHDEAPQVTTIEEAVKHVHTQVENGADYIKIM-----IEEGTVMGV 203 R ++E A H + V + GAD+IK++ + EGT Sbjct: 186 ---MRRGVVEDAADVH-------------RKVRALLVGGADFIKLIATGAVLTEGTE--- 226 Query: 204 PGLPVLSEDILKAAVREAHKLNKIVLAHVLTADSSQSAIRIGVDGLAH-LFIDRPESTSE 262 PG LSE+ ++AAV EA K V AH A+ + A+R GV + H ID E Sbjct: 227 PGQLELSEEEIRAAVEEAAKRGTYVTAHAHGAEGIKIAVRAGVRSIEHGSLID-----DE 281 Query: 263 VVAAIKDSGAFVTPCLVLNASIIGNPASELAGDPRVNSKLSQEWIDILNSSFNTYPQGSL 322 +A +K GAF+ + N I E G P + ++E D F Sbjct: 282 GIALMKAKGAFLV-ADIYNGDFIDTYGRE-HGWPAGMIRKNRETTDAQREGFR------- 332 Query: 323 DNSFKSVMELHKAGVDILVGTDVSPVPLHNLGGLAHGASVHHEMQLLVQAGFTPVEALQS 382 K+V KAGV I GTD P HG + +M +V+ G TP++A+QS Sbjct: 333 ----KAV----KAGVKIAYGTDAGVYP--------HGWNA-RQMPYMVRYGMTPMQAIQS 375 Query: 383 ATSKPARRFGLQDR-GRIAEGLRADLVLVDGDPTTSIS 419 AT+ A G + G A G ADL+ V GDP +S Sbjct: 376 ATTVAAELMGKTGQVGCHAPGCYADLIAVAGDPLADVS 413 >CC0300 [Q] COG1228 Imidazolonepropionase and related amidohydrolases Length = 428 Score = 76.6 bits (187), Expect = 8e-14, Method: Compositional matrix adjust. Identities = 115/453 (25%), Positives = 178/453 (39%), Gaps = 96/453 (21%) Query: 9 PMAGVTAITNARIFDGEKVISARHIIIQEGMIIAVGGEIPAHAAVIDADNATLMPGLIDA 68 P G I + + KV I +G + GG+ V+D ++ ++PGLID+ Sbjct: 37 PATGKVEIAKTLVLENGKVTR-----ILDGYVAEPGGK------VVDLKDSFVLPGLIDS 85 Query: 69 HVHTS-----------------------IGGLRDALKFGVTT--ELEMNGGFTEKGRE-I 102 HVH + G R L G TT L N RE + Sbjct: 86 HVHLTGQQNPNGRLQAVTQSPSDSAMVGAGFARKTLMAGFTTVANLGANSQAIFALREGV 145 Query: 103 QLQNLGGIADVRSAGMAVTAPGGHPDELLPDDGEIPDFVLKELEKLTEKDRNAMLEAFAH 162 + ++ G + ++G +++ GGH D N E H Sbjct: 146 KRGDVAG-PRILASGSSISVHGGH------------------------GDINGYSEDVMH 180 Query: 163 DHDEAPQVTTIEEAVKHVHTQVENGADYIKIMIEEGTVMGVP-GLPV-LSEDILKAAVRE 220 + ++ + V Q+ GAD IKI G + GL S+ L A V Sbjct: 181 ALRPESVCSGADDCRRAVREQIWLGADLIKITATGGVLSNTAAGLAKQFSDAELAAIVES 240 Query: 221 AHKLNKIVLAHVLTADSSQSAIRIGVDGLAH-LFIDRPESTSEVVAAIKDSGAFVTPCLV 279 AH + + V AH D + ++ G D + H ++D +E +A +K SGA++ P L Sbjct: 241 AHSMGRKVTAHAHGGDGINAFLKAGGDSIEHGTYLD-----NEGIALMKKSGAYLVPTL- 294 Query: 280 LNASIIGNPASELAGDPRVNSKLSQEWIDILNSSFNTYPQGSLDNSFKSVMELHKAGVDI 339 + G+ +A P +Q + P+ V H GV + Sbjct: 295 ----MAGDFVYRIASGPNNYFTPAQTA-----KALEAGPK-----MLDMVRRAHADGVKV 340 Query: 340 LVGTDVSPVPLHNLGGLAHGASVHHEMQLLVQAGFTPVEALQSATSKPARRFGLQDR-GR 398 GTD G AHG + E LLV+AG TP+EA+Q+AT A F L + G Sbjct: 341 AFGTDS--------GVSAHGDNAG-EFALLVKAGMTPLEAIQTATVNAADHFSLSNEIGS 391 Query: 399 IAEGLRADLVLVDGDPTTSISDSLSIQAVWLKG 431 + G ADL+ V GDP +++ + +V +KG Sbjct: 392 LTPGKAADLIAVKGDPLKDVTELQRVTSV-IKG 423 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.134 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,160,678 Number of extensions: 1536151 Number of successful extensions: 4469 Number of sequences better than 1.0e-05: 9 Number of HSP's gapped: 4476 Number of HSP's successfully gapped: 10 Length of query: 436 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 327 Effective length of database: 25,807,025 Effective search space: 8438897175 Effective search space used: 8438897175 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)