BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0541.0074.p
         (257 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF0140 [QR] COG0500 SAM-dependent methyltransferases                   72   1e-12
NMA1319 [R] COG4228 Mu-like prophage DNA circulation protein           61   2e-09
TM0318 [QR] COG0500 SAM-dependent methyltransferases                   59   5e-09
STM0318 [R] COG1073 Hydrolases of the alpha/beta superfamily           59   5e-09
MA3655 [H] COG2226 Methylase involved in ubiquinone/menaquinone ...    59   8e-09

>AF0140 [QR] COG0500 SAM-dependent methyltransferases
          Length = 261

 Score = 71.6 bits (174), Expect = 1e-12,   Method: Compositional matrix adjust.
 Identities = 49/148 (33%), Positives = 69/148 (46%), Gaps = 6/148 (4%)

Query: 47  EADTEGSNKKYLEMYNKIARFYRLSNKAYFALKFGGERNYRRQFLSMLELRDGDRVLETS 106
           ++ T  S K     YN+IARFY L       + F   R +R+    +++   G  VLE  
Sbjct: 13  KSTTRKSAKSDRRRYNRIARFYDLFETPMELMNF---RKWRKMLFDLIKAEKG-LVLEIG 68

Query: 107 VGSADNFPYIDAKAEFYGLDISGGMLKQAVRNLRRWRLKAELFQGQAEHLPFQDNRFDCV 166
            G+  N P+  +      LDIS  ML++AVR  +    K +     AE+LPF+ N FD V
Sbjct: 69  AGTGKNIPHYPSNV--VALDISEKMLERAVRRAKESGKKVDFLLADAENLPFRSNSFDVV 126

Query: 167 YHVGGINYFSDPGRAVLEMIRVAKSGTR 194
           +         DP R + E  RV K G R
Sbjct: 127 FTTFVFCSVDDPVRGLREAFRVLKKGGR 154


>NMA1319 [R] COG4228 Mu-like prophage DNA circulation protein
          Length = 199

 Score = 60.8 bits (146), Expect = 2e-09,   Method: Compositional matrix adjust.
 Identities = 45/136 (33%), Positives = 64/136 (47%), Gaps = 8/136 (5%)

Query: 61  YNKIARFYRLSNKAYFALKFGGERNYRRQFLSMLELRDGDRVLETSVGSADNFPYIDAKA 120
           YN+IA  Y L       ++F     +R + LS L+     RVLE  VG+  N  Y  + +
Sbjct: 7   YNRIAAIYDLME---LPMEFFLYGRWREETLSDLQ----GRVLEVGVGTGRNLKYYSSGS 59

Query: 121 EFYGLDISGGMLKQAVRNLRRWRLKAELFQGQAEHLPFQDNRFDCVYHVGGINYFSDPGR 180
              G+D+S GML++A +     +    L    AEHL F D  FD V     +    DP +
Sbjct: 60  SVTGIDVSEGMLEKARKKAEGMK-NVNLLLMDAEHLEFSDKTFDYVITTFVLCSIPDPVK 118

Query: 181 AVLEMIRVAKSGTRLM 196
           A+ EM RV K    L+
Sbjct: 119 ALKEMRRVLKPSGELI 134


>TM0318 [QR] COG0500 SAM-dependent methyltransferases
          Length = 207

 Score = 59.3 bits (142), Expect = 5e-09,   Method: Compositional matrix adjust.
 Identities = 29/94 (30%), Positives = 49/94 (52%), Gaps = 1/94 (1%)

Query: 98  DGDRVLETSVGSADNFPYIDAKAEFYGLDISGGMLKQAVRNLRRW-RLKAELFQGQAEHL 156
           +G ++LE  +G+  N PY     +  G+DIS GML+     L+++   K +L +   ++L
Sbjct: 38  EGKKILEVGIGTGKNVPYYSDDMDVVGVDISEGMLRVCQERLKKFPEKKVKLLRADVQNL 97

Query: 157 PFQDNRFDCVYHVGGINYFSDPGRAVLEMIRVAK 190
           PF D  +DCV          DP + + E+ RV +
Sbjct: 98  PFSDGEYDCVVSTFVFCTVPDPVKGLKEVHRVLR 131


>STM0318 [R] COG1073 Hydrolases of the alpha/beta superfamily
          Length = 414

 Score = 59.3 bits (142), Expect = 5e-09,   Method: Compositional matrix adjust.
 Identities = 4/94 (4%), Positives = 14/94 (14%), Gaps = 1/94 (1%)

Query: 98  DGDRVLETSVGSADNFPYIDAKAEFYGLDISGGMLKQAVRNLRRW-RLKAELFQGQAEHL 156
             D           N      +       +            R    L   +   +  + 
Sbjct: 38  ALDGKNVPHWYRMINRLMWIWRGVDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNW 97

Query: 157 PFQDNRFDCVYHVGGINYFSDPGRAVLEMIRVAK 190
            ++  +    +                 +     
Sbjct: 98  IYEWAKQAMDWQQKACQEQDAMRSGRYWLHASTL 131


>MA3655 [H] COG2226 Methylase involved in ubiquinone/menaquinone
           biosynthesis
          Length = 226

 Score = 58.9 bits (141), Expect = 8e-09,   Method: Compositional matrix adjust.
 Identities = 49/148 (33%), Positives = 80/148 (54%), Gaps = 21/148 (14%)

Query: 61  YNKIARFYRLSNKAYFALKFGGERNYRRQFLSMLELRDGDRVLETSVGSADNFPYI---D 117
           ++K+A FY L ++      F  E+   +  L M ++++G++VLE +VG+   F +I   +
Sbjct: 25  FSKVAWFYDLWSR------FAEEKAVNKA-LEMSKIKNGEKVLEVAVGTGIMFEHIVKLN 77

Query: 118 AKAEFYGLDISGGMLKQAVRNLRRWRLKA-ELFQGQAEHLPFQDNRFDCVYHVGGINYFS 176
            + E  G+DIS  MLK+A + L+    K   L +G A +L +QD  FD + +    NY  
Sbjct: 78  PEGENEGIDISDDMLKRAKKRLKDVNNKNYNLNEGNALNLQYQDQYFDVLIN----NYML 133

Query: 177 D--PGRAVLEMI----RVAKSGTRLMIA 198
           D  PG  + E++    RV K G RL+I 
Sbjct: 134 DLFPGEKIDEVLREFNRVLKPGGRLVIT 161


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.323    0.142    0.430 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 19,867,014
Number of extensions: 823047
Number of successful extensions: 1435
Number of sequences better than 1.0e-05: 12
Number of HSP's gapped: 1451
Number of HSP's successfully gapped: 12
Length of query: 257
Length of database: 38,378,431
Length adjustment: 104
Effective length of query: 153
Effective length of database: 26,383,695
Effective search space: 4036705335
Effective search space used: 4036705335
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 114 (48.5 bits)