BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0069.p (1950 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3700 [C] COG0584 Glycerophosphoryl diester phosphodiesterase 107 1e-22 SA1542 [C] COG0584 Glycerophosphoryl diester phosphodiesterase 103 4e-21 BS_glpQ [C] COG0584 Glycerophosphoryl diester phosphodiesterase 100 4e-20 BS_yhdW [C] COG0584 Glycerophosphoryl diester phosphodiesterase 99 6e-20 BH1080 [C] COG0584 Glycerophosphoryl diester phosphodiesterase 99 8e-20 >BH3700 [C] COG0584 Glycerophosphoryl diester phosphodiesterase Length = 284 Score = 107 bits (268), Expect = 1e-22, Method: Composition-based stats. Identities = 70/241 (29%), Positives = 122/241 (50%), Gaps = 10/241 (4%) Query: 1138 RKVYIIGHRGMPSVSPENTIESNRLALDAGADFIENDMFLTTDGHLIITHSPVLETTTDG 1197 +K+ I HRG +PENT+ + + D D+IE D+ +T DG L++ H ++ TT+G Sbjct: 37 QKMINIAHRGASGHAPENTMAAFQKGFDMKVDYIEFDVHMTKDGELVLMHDLTVDRTTNG 96 Query: 1198 SGPVENYSLEQLKTYNANKTYPEGFPLVRMPTLDEQLELARSR-GRLVYAEIKTQTPEAV 1256 G V + +L+++K ++A + E F ++PT +E L++ R + G L+ + + P Sbjct: 97 HGYVSDLTLDEIKQFDAGSWFHEDFVGEKVPTFEETLDVFRGKVGLLIELKSPERYPGME 156 Query: 1257 DAFVRTVKEHKAEDIVN----VMSFYPAQLERLNKLMPEIPAGLLTGYIASENNVEGSLR 1312 + +KE K + N + SF +++ +L+P IP G+L G S NV Sbjct: 157 EKVAEALKERKMDRSSNHKVIIQSFNHESMKKSKELLPHIPHGVLAG--PSWANVTD--- 211 Query: 1313 DTLKTVSKLNLTFNTDYSGLGPEFMEAAKHRGLLISPWTLNKREDVIRFLKLGAFGITTD 1372 + L+ S FN + + + PE + G+ I P+T +E R +L GI TD Sbjct: 212 EQLEEFSTYADYFNPNMTIVTPELVSRIHAVGMKIFPYTSRTQEQANRLFELKVDGIITD 271 Query: 1373 Y 1373 + Sbjct: 272 F 272 >SA1542 [C] COG0584 Glycerophosphoryl diester phosphodiesterase Length = 247 Score = 103 bits (256), Expect = 4e-21, Method: Composition-based stats. Identities = 67/237 (28%), Positives = 120/237 (50%), Gaps = 8/237 (3%) Query: 1139 KVYIIGHRGMPSVSPENTIESNRLALDAGADFIENDMFLTTDGHLIITHSPVLETTTDGS 1198 ++ I+ HRG+PS PENT+ R + +E D+ LT D H ++ H ++ T+DG Sbjct: 9 ELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEIDVHLTKDQHFVVIHDETIDRTSDGK 68 Query: 1199 GPVENYSLEQLKTYNANKTYPEGFPLVRMPTLDEQLELARSRGRLVYAEIKTQT--PEAV 1256 G + +Y+L QLK+++ F R+PTLDE L L + + E+K+ PE Sbjct: 69 GRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDEVLSLCLKYDKKLLIELKSPNLYPEIE 128 Query: 1257 DAFVRTVKEHKAEDI-VNVMSFYPAQLERLNKLMPEIPAGLLTGYIASENNVEGSLRDTL 1315 + ++E K + V + SF +E+LN L G+L+ S+ N + Sbjct: 129 CKLLAFLEEKKVDATQVVIQSFDIECIEKLNTLGSIYELGVLS----SKRNYWYK-KPNF 183 Query: 1316 KTVSKLNLTFNTDYSGLGPEFMEAAKHRGLLISPWTLNKREDVIRFLKLGAFGITTD 1372 ++++ N +Y+ + +F++ A H L + P+T+NK + + ++G G+ TD Sbjct: 184 SRIAQIASYVNPNYALVARKFVDKAHHHQLQVMPYTVNKLKTGEKLRQMGVDGLITD 240 >BS_glpQ [C] COG0584 Glycerophosphoryl diester phosphodiesterase Length = 293 Score = 99.8 bits (247), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 74/254 (29%), Positives = 125/254 (49%), Gaps = 27/254 (10%) Query: 1139 KVYIIGHRGMPSVSPENTIESNRLALDAGADFIENDMFLTTDGHLIITHSPVLETTTDGS 1198 ++ + HRG PE+TI S A ADFIE D+ +T DG LI+ H L+ TT+G Sbjct: 37 RILTVAHRGASGYVPEHTILSYETAQKMKADFIELDLQMTKDGKLIVMHDEKLDRTTNGM 96 Query: 1199 GPVENYSLEQLKTYNA----NKTYPEG----FPLVRMPTLDEQLELARSRGRLVYAEIKT 1250 G V++++L +K +A N+ YPE + +++PTL+E L+ G+ I+T Sbjct: 97 GWVKDHTLADIKKLDAGSWFNEAYPEKAKPQYVGLKVPTLEEVLD---RFGKHANYYIET 153 Query: 1251 QTPEAV----DAFVRTVKEHK------AEDIVNVMSFYPAQLERLNKLMPEIPAGLLTGY 1300 ++P+ + + ++++HK V + SF L ++++L P +P L Sbjct: 154 KSPDTYPGMEEKLIASLQKHKLLGKHSKPGQVIIQSFSKESLVKVHQLQPNLPTVQLL-- 211 Query: 1301 IASENNVEGSLRD-TLKTVSKLNLTFNTDYSGLGPEFMEAAKHRGLLISPWTLNKREDVI 1359 E S+ D L+ + + DY L E + + GLL+ P+T+N D+ Sbjct: 212 ---EAKQMASMTDAALEEIKTYAVGAGPDYKALNQENVRMIRSHGLLLHPYTVNNEADMH 268 Query: 1360 RFLKLGAFGITTDY 1373 R L G G+ T+Y Sbjct: 269 RLLDWGVTGVFTNY 282 >BS_yhdW [C] COG0584 Glycerophosphoryl diester phosphodiesterase Length = 305 Score = 99.4 bits (246), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 18/237 (7%), Positives = 43/237 (18%), Gaps = 10/237 (4%) Query: 1140 VYIIGHRGMPSVSPENTIESNRLALDAGADFIENDMFLTTDGHLIITHSPVLETTTDGSG 1199 + G+P S + + +F + T SG Sbjct: 1 MQCGISDGLPGFSYADADGKFSGIDVDICRGVAAAVFGDDTKVKYTPLTAKERFTALQSG 60 Query: 1200 PVENYSLEQLKTYNANKTYPEGFPLVRMPTLDEQLELARSR-GRLVYAEIKTQTPEA-VD 1257 V+ S T + + F V L ++ E+ T Sbjct: 61 EVDLLSRNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAK--ELDGATVCIQAG 118 Query: 1258 AFVRTVKEHKAEDIVNVM-SFYPAQLERLNKLMPEIPAGLLTGYIASENNVEGSLRDTLK 1316 + + + K + L + + Sbjct: 119 TDTELNVADYFKANNMKYTPVTFDRSDESAKALESGRCDTLA-----SDQSQLYALRIKL 173 Query: 1317 TVSKLNLTFNTDYSGLGPEFMEAAKHRGLLISPWTLNKREDVIRFLKLGAFGITTDY 1373 + + S + + + + + Sbjct: 174 SNPAEWIVLPEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKA 230 >BH1080 [C] COG0584 Glycerophosphoryl diester phosphodiesterase Length = 250 Score = 99.0 bits (245), Expect = 8e-20, Method: Compositional matrix adjust. Identities = 76/242 (31%), Positives = 117/242 (48%), Gaps = 19/242 (7%) Query: 1138 RKVYIIGHRGMPSVSPENTIESNRLALDAGADFIENDMFLTTDGHLIITHSPVLETTTDG 1197 ++ +I HRG+ PENT+ + R A GAD IE D+ LT D LI+ H L TT G Sbjct: 5 QRTHIYAHRGVSCKYPENTLSAFRAAEGVGADGIELDVQLTKDDQLIVIHDLHLNRTTSG 64 Query: 1198 SGPVENYSLEQLKTYNANKTYPEGFPLVRMPTLDEQLELARSRGRLVYAEIKTQTPE--- 1254 SG V ++L +LK +A + E F +PTLDE A+S + E+K + Sbjct: 65 SGLVRAHTLSELKRLDAGSWFDERFTGETLPTLDEVFAWAQSNELIFNVELKGVVEDRDR 124 Query: 1255 ---AVDAFVRTVKEHKAEDIVNVMSFYPAQLERLNKLMPEIPAGLLTGYIASENNVEGSL 1311 A+ A + T K D V + SF +++L+ ++ V +L Sbjct: 125 LFTAISACIDTF---KMRDRVILSSFDHVLIKKLSGSFTRNQLAVI---------VMAAL 172 Query: 1312 RDTLKTVSKLNLT-FNTDYSGLGPEFMEAAKHRGLLISPWTLNKREDVIRFLKLGAFGIT 1370 D V K+ T ++ Y + + H+GL + P+TLN+ ED+ R+++LG GI Sbjct: 173 YDPGGYVQKVGATGYHFYYLAMTEAEVYHLLHKGLKLRPFTLNQEEDIRRYMQLGVNGIF 232 Query: 1371 TD 1372 TD Sbjct: 233 TD 234 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.132 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 136,053,221 Number of extensions: 5633138 Number of successful extensions: 10566 Number of sequences better than 1.0e-05: 58 Number of HSP's gapped: 10527 Number of HSP's successfully gapped: 58 Length of query: 1950 Length of database: 38,378,431 Length adjustment: 121 Effective length of query: 1829 Effective length of database: 24,423,017 Effective search space: 44669698093 Effective search space used: 44669698093 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 124 (52.4 bits)