BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0066.p (443 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value TM1120 [G] COG1653 ABC-type sugar transport system, periplasmic ... 180 3e-45 ugpB [G] COG1653 ABC-type sugar transport system, periplasmic co... 153 6e-37 ZugpB [G] COG1653 ABC-type sugar transport system, periplasmic c... 153 6e-37 ECs4299 [G] COG1653 ABC-type sugar transport system, periplasmic... 153 6e-37 >TM1120 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 436 Score = 180 bits (457), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 125/402 (31%), Positives = 203/402 (50%), Gaps = 27/402 (6%) Query: 46 KTELTYYYPIAVGGPLTATIEAMTADFMKENPDIVVKPVYTGSYADTAVKTQAGVQSKQP 105 K ++ +++ A+GG ++ M DFMK +PDI V+ YTGSY DT K A VQ P Sbjct: 19 KVKIQFWH--AMGGWRIELLQNMAEDFMKTHPDIEVEVQYTGSYRDTLNKLVAAVQGGTP 76 Query: 106 PDVAVLQSTELFSLLDMDAIIPLDEFIAQEGGDTYLSDFYPAFMENSQTEGKTYSIPFQR 165 P V + ++D +P+ + I ++ + F P ++ + +GK YS+PF Sbjct: 77 PHVVQIYEIGTQFMIDSGIAVPIGDLIEKDPSFD-VGKFLPQVLDYYRVKGKLYSMPFNS 135 Query: 166 STIVLYYNKDMFKAAGLDPEKPPATWAEMQEYAVKLA-----GN-GKWGLEIPVTGYAYW 219 S +LYYNK +FK GLDP KPP T+ E+ EY KL GN + G+ P+ ++++ Sbjct: 136 SNPILYYNKTLFKEVGLDPNKPPRTFNELIEYCKKLTVKDEKGNIVRAGITWPL--HSWF 193 Query: 220 MLQTFALQNGNNLMTPDGK-----KVMFNTPENVEGLQYWVDLAAKHKAMPGGVTDWSTV 274 Q ALQN + +G+ K +FN + L+ W L + + DW+ Sbjct: 194 FEQFVALQNAPLVDNENGRAGRATKAVFNHEGALRFLKLWDTLTKEGLMINTTKEDWTGA 253 Query: 275 PSDFLEGKTAMMYHTTGN---LTNVKKNASFDFGVSFLPAQKQY---GSPTGGGNFYIFK 328 F+ K AM+ +T + + + K F+ G +FLP + G+P GGG+ +I Sbjct: 254 RQLFISQKVAMLISSTSDVKLMMDAAKENGFELGTAFLPKPEGVELGGTPIGGGSLWIIG 313 Query: 329 DIDKKKQEAAWKFVKFMTETERAAQWSIDTGYVAVKKSAYETERMKAYAAEFPAALIARD 388 +++ +AAW+FVK+M E E+ +W + TGY V+K A ET + Y +E+P L A Sbjct: 314 GHPEEEIKAAWEFVKWMAEPEQQIRWHLGTGYFPVRKDAVETLLYQGYYSEYPHHLTALL 373 Query: 389 QLEYASAELSTHNN-----GRIVKILSDSVQAALTNELTPAE 425 QL + +T + I+ +++ + E+TP E Sbjct: 374 QLLLSVQTPNTRGAVIGPFPEVRDIIETAIEKMINGEMTPEE 415 >ugpB [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 438 Score = 153 bits (386), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 117/390 (30%), Positives = 187/390 (47%), Gaps = 31/390 (7%) Query: 59 GPLTATIEAMTADFMKENPDIVVKPVYTGSYADTAVKTQAGVQSKQPPDVAVLQSTEL-- 116 G L ++++ F ENPD + P Y G+Y A ++ P A+LQ E+ Sbjct: 35 GELGKEVDSLAQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAP--AILQVYEVGT 92 Query: 117 FSLLDMDAIIPLDEFIAQEGGDTYLSDFYP---AFMENSQTEGKTYSIPFQRSTIVLYYN 173 +++ AI P+ + + G S F P + +S+T G S PF ST VLYYN Sbjct: 93 ATMMASKAIKPVYDVFKEAGIQFDESQFVPTVSGYYSDSKT-GHLLSQPFNSSTPVLYYN 151 Query: 174 KDMFKAAGLDPEKPPATWAEMQEYAVKLAGNG-KWGLEIPVTGYAYWM-LQTFALQNG-- 229 KD FK AGLDPE+PP TW ++ +YA KL +G K G +G+ W+ L+ F+ NG Sbjct: 152 KDAFKKAGLDPEQPPKTWQDLADYAAKLKASGMKCGY---ASGWQGWIQLENFSAWNGLP 208 Query: 230 ----NNLMTPDGKKVMFNTPENVEGLQYWVDLAAKHKAMPGGVTDWSTVPSDFLEGKTAM 285 NN + FN PE V+ + ++ K G D ST F G AM Sbjct: 209 FASKNNGFDGTDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDEST--EKFYNGDCAM 266 Query: 286 MYHTTGNLTNVKKNASFDFGVSFLP-----AQKQYGSPTGGGNFYIFKDIDKKKQEAAWK 340 ++G+L N+++ A F++GV +P + GG + ++ + DK+ K Sbjct: 267 TTASSGSLANIREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAK 326 Query: 341 FVKFMTETERAAQWSIDTGYVAVKKSAYETERMKAYAAEFPAALIARDQLEYA-----SA 395 F+ F+ + E AA+W TGY+ + K+AY+ R + + + P A A Q+ + Sbjct: 327 FLDFLAKPENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTK 386 Query: 396 ELSTHNNGRIVKILSDSVQAALTNELTPAE 425 L N +I I+ + +++ T + TP + Sbjct: 387 GLRLGNMPQIRVIVDEELESVWTGKKTPQQ 416 >ZugpB [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 438 Score = 153 bits (386), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 117/390 (30%), Positives = 187/390 (47%), Gaps = 31/390 (7%) Query: 59 GPLTATIEAMTADFMKENPDIVVKPVYTGSYADTAVKTQAGVQSKQPPDVAVLQSTEL-- 116 G L ++++ F ENPD + P Y G+Y A ++ P A+LQ E+ Sbjct: 35 GELGKEVDSLAQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAP--AILQVYEVGT 92 Query: 117 FSLLDMDAIIPLDEFIAQEGGDTYLSDFYP---AFMENSQTEGKTYSIPFQRSTIVLYYN 173 +++ AI P+ + + G S F P + +S+T G S PF ST VLYYN Sbjct: 93 ATMMASKAIKPVYDVFKEAGIQFDESQFVPTVSGYYSDSKT-GHLLSQPFNSSTPVLYYN 151 Query: 174 KDMFKAAGLDPEKPPATWAEMQEYAVKLAGNG-KWGLEIPVTGYAYWM-LQTFALQNG-- 229 KD FK AGLDPE+PP TW ++ +YA KL +G K G +G+ W+ L+ F+ NG Sbjct: 152 KDAFKKAGLDPEQPPKTWQDLADYAAKLKASGMKCGY---ASGWQGWIQLENFSAWNGLP 208 Query: 230 ----NNLMTPDGKKVMFNTPENVEGLQYWVDLAAKHKAMPGGVTDWSTVPSDFLEGKTAM 285 NN + FN PE V+ + ++ K G D ST F G AM Sbjct: 209 FASKNNGFDGTDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDEST--EKFYNGDCAM 266 Query: 286 MYHTTGNLTNVKKNASFDFGVSFLP-----AQKQYGSPTGGGNFYIFKDIDKKKQEAAWK 340 ++G+L N+++ A F++GV +P + GG + ++ + DK+ K Sbjct: 267 TTASSGSLANIREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAK 326 Query: 341 FVKFMTETERAAQWSIDTGYVAVKKSAYETERMKAYAAEFPAALIARDQLEYA-----SA 395 F+ F+ + E AA+W TGY+ + K+AY+ R + + + P A A Q+ + Sbjct: 327 FLDFLAKPENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTK 386 Query: 396 ELSTHNNGRIVKILSDSVQAALTNELTPAE 425 L N +I I+ + +++ T + TP + Sbjct: 387 GLRLGNMPQIRVIVDEELESVWTGKKTPQQ 416 Score = 153 bits (386), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 117/390 (30%), Positives = 187/390 (47%), Gaps = 31/390 (7%) Query: 59 GPLTATIEAMTADFMKENPDIVVKPVYTGSYADTAVKTQAGVQSKQPPDVAVLQSTEL-- 116 G L ++++ F ENPD + P Y G+Y A ++ P A+LQ E+ Sbjct: 35 GELGKEVDSLAQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAP--AILQVYEVGT 92 Query: 117 FSLLDMDAIIPLDEFIAQEGGDTYLSDFYP---AFMENSQTEGKTYSIPFQRSTIVLYYN 173 +++ AI P+ + + G S F P + +S+T G S PF ST VLYYN Sbjct: 93 ATMMASKAIKPVYDVFKEAGIQFDESQFVPTVSGYYSDSKT-GHLLSQPFNSSTPVLYYN 151 Query: 174 KDMFKAAGLDPEKPPATWAEMQEYAVKLAGNG-KWGLEIPVTGYAYWM-LQTFALQNG-- 229 KD FK AGLDPE+PP TW ++ +YA KL +G K G +G+ W+ L+ F+ NG Sbjct: 152 KDAFKKAGLDPEQPPKTWQDLADYAAKLKASGMKCGY---ASGWQGWIQLENFSAWNGLP 208 Query: 230 ----NNLMTPDGKKVMFNTPENVEGLQYWVDLAAKHKAMPGGVTDWSTVPSDFLEGKTAM 285 NN + FN PE V+ + ++ K G D ST F G AM Sbjct: 209 FASKNNGFDGTDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDEST--EKFYNGDCAM 266 Query: 286 MYHTTGNLTNVKKNASFDFGVSFLP-----AQKQYGSPTGGGNFYIFKDIDKKKQEAAWK 340 ++G+L N+++ A F++GV +P + GG + ++ + DK+ K Sbjct: 267 TTASSGSLANIREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAK 326 Query: 341 FVKFMTETERAAQWSIDTGYVAVKKSAYETERMKAYAAEFPAALIARDQLEYA-----SA 395 F+ F+ + E AA+W TGY+ + K+AY+ R + + + P A A Q+ + Sbjct: 327 FLDFLAKPENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTK 386 Query: 396 ELSTHNNGRIVKILSDSVQAALTNELTPAE 425 L N +I I+ + +++ T + TP + Sbjct: 387 GLRLGNMPQIRVIVDEELESVWTGKKTPQQ 416 >ECs4299 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 438 Score = 153 bits (386), Expect = 6e-37, Method: Compositional matrix adjust. Identities = 117/390 (30%), Positives = 187/390 (47%), Gaps = 31/390 (7%) Query: 59 GPLTATIEAMTADFMKENPDIVVKPVYTGSYADTAVKTQAGVQSKQPPDVAVLQSTEL-- 116 G L ++++ F ENPD + P Y G+Y A ++ P A+LQ E+ Sbjct: 35 GELGKEVDSLAQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAP--AILQVYEVGT 92 Query: 117 FSLLDMDAIIPLDEFIAQEGGDTYLSDFYP---AFMENSQTEGKTYSIPFQRSTIVLYYN 173 +++ AI P+ + + G S F P + +S+T G S PF ST VLYYN Sbjct: 93 ATMMASKAIKPVYDVFKEAGIQFDESQFVPTVSGYYSDSKT-GHLLSQPFNSSTPVLYYN 151 Query: 174 KDMFKAAGLDPEKPPATWAEMQEYAVKLAGNG-KWGLEIPVTGYAYWM-LQTFALQNG-- 229 KD FK AGLDPE+PP TW ++ +YA KL +G K G +G+ W+ L+ F+ NG Sbjct: 152 KDAFKKAGLDPEQPPKTWQDLADYAAKLKASGMKCGY---ASGWQGWIQLENFSAWNGLP 208 Query: 230 ----NNLMTPDGKKVMFNTPENVEGLQYWVDLAAKHKAMPGGVTDWSTVPSDFLEGKTAM 285 NN + FN PE V+ + ++ K G D ST F G AM Sbjct: 209 FASKNNGFDGTDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDEST--EKFYNGDCAM 266 Query: 286 MYHTTGNLTNVKKNASFDFGVSFLP-----AQKQYGSPTGGGNFYIFKDIDKKKQEAAWK 340 ++G+L N+++ A F++GV +P + GG + ++ + DK+ K Sbjct: 267 TTASSGSLANIREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAK 326 Query: 341 FVKFMTETERAAQWSIDTGYVAVKKSAYETERMKAYAAEFPAALIARDQLEYA-----SA 395 F+ F+ + E AA+W TGY+ + K+AY+ R + + + P A A Q+ + Sbjct: 327 FLDFLAKPENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTK 386 Query: 396 ELSTHNNGRIVKILSDSVQAALTNELTPAE 425 L N +I I+ + +++ T + TP + Sbjct: 387 GLRLGNMPQIRVIVDEELESVWTGKKTPQQ 416 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.130 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 29,307,018 Number of extensions: 1228019 Number of successful extensions: 4080 Number of sequences better than 1.0e-05: 64 Number of HSP's gapped: 3966 Number of HSP's successfully gapped: 65 Length of query: 443 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 333 Effective length of database: 25,691,691 Effective search space: 8555333103 Effective search space used: 8555333103 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 117 (49.7 bits)