BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0064.p (281 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value TM0598 [G] COG0395 ABC-type sugar transport system, permease com... 129 7e-30 STM0598 [IQR] COG1028 Dehydrogenases with different specificitie... 129 7e-30 lin0219 [G] COG0395 ABC-type sugar transport system, permease co... 120 2e-27 Cgl1351 [G] COG0395 ABC-type sugar transport system, permease co... 119 5e-27 all4824 [G] COG0395 ABC-type sugar transport system, permease co... 116 3e-26 >TM0598 [G] COG0395 ABC-type sugar transport system, permease component Length = 277 Score = 129 bits (323), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 72/220 (32%), Positives = 111/220 (50%), Gaps = 2/220 (0%) Query: 62 NFATVWSAAPFATYYLNTILIVVCVFAVQMLTXXXXXXXXXXXXXXXXXIVFLLFLVQIM 121 N+ W+AAPF YY NT + ++++T +F LFL +M Sbjct: 60 NYVDAWNAAPFPRYYFNTFFVATTTTLLEVVTASLAAYAFSWMVFPGRDFIFGLFLATMM 119 Query: 122 IPADVLIFPNYNVLKQLSLLDTKLGIMLPFLASAFGIFLLRQVFKTIPXXXXXXXXIEGS 181 IP +VL+ PN+ + +L +DT +++P++ S F IFLLRQ F TIP I+G Sbjct: 120 IPGEVLLVPNFITISKLGWIDTYYALIIPWIVSVFAIFLLRQHFLTIPRELFDAAKIDGC 179 Query: 182 SQWQVLWKIYFPLARPTYVAFALVSISYQWNNFLWPLIITNSVENRPLTVGLSIFAQSFE 241 S W+ LW++ PL++P + AL+ WN FLW LI+TNS + R L VGL F+ + Sbjct: 180 SHWRFLWQMVVPLSKPAVITSALLKFVGSWNAFLWVLIVTNSEKYRTLPVGLQAFSS--D 237 Query: 242 IGVQWTEVSXXXXXXXXXXXXXXXXXQRQFIESFMHSGIK 281 +G Q+ + Q+ FI+ +G+K Sbjct: 238 VGTQYNLLMAAATFSILPVVILFIFTQKYFIQGIARTGLK 277 >STM0598 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 251 Score = 129 bits (323), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 0/220 (0%), Positives = 0/220 (0%), Gaps = 2/220 (0%) Query: 62 NFATVWSAAPFATYYLNTILIVVCVFAVQMLTXXXXXXXXXXXXXXXXXIVFLLFLVQIM 121 +L+ T F LF + Sbjct: 60 AQVAQVCQRVLQKTTRLDVLVNAAGILRMGATDALSVDDWQQTFAVNVGGAFNLFSQTMA 119 Query: 122 IPADVLIFPNYNVLKQLSLLDTKLGIMLPFLASAFGIFLLRQVFKTIPXXXXXXXXIEGS 181 V + +A L + GS Sbjct: 120 QFRRQQGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALTVGLELAGCGVRCNVVSPGS 179 Query: 182 SQWQVLWKIYFPLARPTYVAFALVSISYQWNNFLWPLIITNSVENRPLTVGLSIFAQSFE 241 + + ++ Sbjct: 180 TDTDMQRTLWVSEDAEQQRIRGFGEQFKLGIPLGKIARPQEIANTILFLASDLASHI--T 237 Query: 242 IGVQWTEVSXXXXXXXXXXXXXXXXXQRQFIESFMHSGIK 281 Sbjct: 238 277 >lin0219 [G] COG0395 ABC-type sugar transport system, permease component Length = 282 Score = 120 bits (301), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 77/266 (28%), Positives = 126/266 (47%), Gaps = 12/266 (4%) Query: 19 LGNILMLALAAFWLIPLLWMTVTAFRPRHAAL---SGLFSLDFTLGNFATVWSAAPFATY 75 LG I+ML +P +WM TAF+ A + + T NF V+ P + Sbjct: 25 LGGIIML-------MPFVWMISTAFKTGAANMVLPPQFIPKEPTTANFTQVFEMFPMLRF 77 Query: 76 YLNTILIVVCVFAVQMLTXXXXXXXXXXXXXXXXXIVFLLFLVQIMIPADVLIFPNYNVL 135 +N++++ V QML +FLL+L +M+PA V + P + ++ Sbjct: 78 LMNSVVVAVVTTLGQMLFCSMAAYAFARIPFWGRDKLFLLYLATMMVPAQVTMIPQFILM 137 Query: 136 KQLSLLDTKLGIMLPFLASAFGIFLLRQVFKTIPXXXXXXXXIEGSSQWQVLWKIYFPLA 195 KQ LD+ G+++P L S FG FLLRQ F IP ++G++ + + K+ PLA Sbjct: 138 KQFGWLDSYAGLIVPALFSVFGTFLLRQAFMGIPKELEEAAFMDGANHFTIFRKVILPLA 197 Query: 196 RPTYVAFALVSISYQWNNFLWPLIITNSVENRPLTVGLSIFAQSFEIGVQWTEVSXXXXX 255 +PT+ +++ WN++LWPLI+T+S E L +GLS+ + G + + Sbjct: 198 KPTFATLGILTFMQSWNSYLWPLIVTSSQEMATLPLGLSLLQGRY--GTNYGLMMAGVLI 255 Query: 256 XXXXXXXXXXXXQRQFIESFMHSGIK 281 Q+ FI+ SG+K Sbjct: 256 SVIPILAVYLFAQKYFIQGMAMSGMK 281 >Cgl1351 [G] COG0395 ABC-type sugar transport system, permease component Length = 278 Score = 119 bits (299), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 70/273 (25%), Positives = 133/273 (48%), Gaps = 4/273 (1%) Query: 12 KTKLTSRLGNILMLALAAFWLIPLLWMTVTAFRPRHAALSGL---FSLDFTLGNFATVWS 68 + L +G + M+ F +PL+++ +T+F+ + + F +F N+A V+ Sbjct: 6 RNVLVKIMGYVGMVLAILFIGLPLVFIVLTSFKQQSEIYTQPVTWFPSEFNFDNYANVFE 65 Query: 69 AAPFATYYLNTILIVVCVFAVQMLTXXXXXXXXXXXXXXXXXIVFLLFLVQIMIPADVLI 128 PF Y+ N+I+I V + V+++ +VFLL + +M+P++V + Sbjct: 66 RVPFLNYFRNSIIITVILCLVKIILGVISAYALSILRFPGRNLVFLLVISALMVPSEVTV 125 Query: 129 FPNYNVLKQLSLLDTKLGIMLPFLASAFGIFLLRQVFKTIPXXXXXXXXIEGSSQWQVLW 188 NY ++ QL DT GI++P AFG FL+R F +IP ++ +++LW Sbjct: 126 ISNYALVSQLGWRDTYQGIIVPLAGIAFGTFLMRNHFMSIPSELIEAARMDHCGHFRLLW 185 Query: 189 KIYFPLARPTYVAFALVSISYQWNNFLWPLIITNSVENRPLTVGLSIFAQSFEIGVQWTE 248 K+ P++ PT VAF+++++ +WN +LWP ++ + + L +GL++ Q+ E W Sbjct: 186 KVLLPISMPTLVAFSMITVVNEWNQYLWPFLMAETDNSATLPIGLTML-QNNEGVSNWGP 244 Query: 249 VSXXXXXXXXXXXXXXXXXQRQFIESFMHSGIK 281 V Q+ I+ + +K Sbjct: 245 VMAATIMTMLPVLVMFLALQQYMIKGLISGAVK 277 >all4824 [G] COG0395 ABC-type sugar transport system, permease component Length = 279 Score = 116 bits (291), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 78/254 (30%), Positives = 116/254 (45%), Gaps = 6/254 (2%) Query: 32 LIPLLWMTVTAFRPRHAAL----SGLFSLDFTLGNFATVWSAAPFATYYLNTILIVVCVF 87 L PLLW+ TA + + L T+ NF VW++ PF Y N+ L+ V Sbjct: 27 LFPLLWLISTALKSPTENIFQSPPQLLPNQPTVDNFFKVWNSLPFGQYLYNSTLVSVLTV 86 Query: 88 AVQMLTXXXXXXXXXXXXXXXXXIVFLLFLVQIMIPADVLIFPNYNVLKQLSLLDTKLGI 147 + +L +F+ + IMIP +++ P Y + QL L +T LG+ Sbjct: 87 GLNLLFCSLAAYPLARLSFPGRDWIFIAIVSTIMIPFQIVMIPLYILTVQLGLRNTYLGM 146 Query: 148 MLPFLASAFGIFLLRQVFKTIPXXXXXXXXIEGSSQWQVLWKIYFPLARPTYVAFALVSI 207 M P LASAFGIFLLRQ F +P ++GSS+ + W I P RP V A+ Sbjct: 147 MFPSLASAFGIFLLRQAFMGVPKEIEEAARMDGSSELGLWWHIMLPAVRPALVTLAIFVF 206 Query: 208 SYQWNNFLWPLIITNSVENRPLTVGLSIFAQSFEIGVQWTEVSXXXXXXXXXXXXXXXXX 267 W++FLWPLI+ L +G++ A +F + W V+ Sbjct: 207 IGAWSDFLWPLIVIQDENLYTLPLGVAKLAGTFSL--DWRLVAAGSVIAIAPVLLLFLIL 264 Query: 268 QRQFIESFMHSGIK 281 QR +E+ + SG+K Sbjct: 265 QRYIVETDIGSGVK 278 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.330 0.141 0.445 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,964,968 Number of extensions: 496767 Number of successful extensions: 1546 Number of sequences better than 1.0e-05: 132 Number of HSP's gapped: 1430 Number of HSP's successfully gapped: 132 Length of query: 281 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 176 Effective length of database: 26,268,361 Effective search space: 4623231536 Effective search space used: 4623231536 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 115 (48.9 bits)