BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0061.p (509 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1874 [G] COG3534 Alpha-L-arabinofuranosidase 718 0.0 BS_xsa [G] COG3534 Alpha-L-arabinofuranosidase 675 0.0 BS_abfA [G] COG3534 Alpha-L-arabinofuranosidase 164 4e-40 BH1861 [G] COG3534 Alpha-L-arabinofuranosidase 156 7e-38 >BH1874 [G] COG3534 Alpha-L-arabinofuranosidase Length = 498 Score = 718 bits (1854), Expect = 0.0, Method: Compositional matrix adjust. Identities = 330/498 (66%), Positives = 400/498 (80%), Gaps = 2/498 (0%) Query: 12 MAQRVVLHTDIRKGTINRNIYGHFSEHLGRCIYEGIWVGEDSPIPNTKGIRNDVVEALKE 71 M Q + ++TD+ G I+++IYGHFSEHLGRCIYEG+WVGEDSPIPNT+GIRNDV+ AL+E Sbjct: 1 MKQSIKVYTDLEHGKISKHIYGHFSEHLGRCIYEGLWVGEDSPIPNTEGIRNDVLGALQE 60 Query: 72 IKVPVLRWPGGCFADEYHWKDGIGAREGRKRMINTHWGGTVENNHFGTHEFMELCAMLEC 131 + +PVLRWPGGCFADEYHWKDG+G RE RKRM+NTHWGG VENNHFGTHEFM LC +L Sbjct: 61 LNIPVLRWPGGCFADEYHWKDGVGPRENRKRMVNTHWGGVVENNHFGTHEFMRLCELLGA 120 Query: 132 EPYINGNVGSGTVQEMSEWVEYLTFNGVSPMAELRAKNGREEAWAVKYFGVGNENWGCGG 191 EPYI GNVGSGTVQEM EWVEY+TF+G SPMA R +NGREE WA+KYFGVGNENWGCGG Sbjct: 121 EPYICGNVGSGTVQEMQEWVEYMTFDGESPMANWRTENGREEPWALKYFGVGNENWGCGG 180 Query: 192 NMRPEYYADLYRQYQTYVRNYGDNRIHRIACGPNADDYQWMEVLMREATRFMDSITLHYY 251 +MRPEYYADLYR+YQTYVRNYGDN+I++IA G N DDY+W EVLMREA MD ++LHYY Sbjct: 181 HMRPEYYADLYRRYQTYVRNYGDNKIYKIAGGANVDDYRWTEVLMREAAHLMDGLSLHYY 240 Query: 252 TVPGATWEHKGAATGFDTAEYFTTLEKALRMEELVTRHSVIMDKYDPEKRVGLIVDEWGT 311 TVPG WE+KG+A +E+F TL+K+ RM+EL+T+HS IMD+YDPEKRVGLIVDEWGT Sbjct: 241 TVPGDFWENKGSALDDRESEWFKTLKKSFRMDELLTKHSTIMDRYDPEKRVGLIVDEWGT 300 Query: 312 WYDVEPGTNPGFLYQQNSIRDALVAGLTLNIFHKHSDRVRMANIAQTINVLQAVILTEGE 371 W+DVEPGTNPGFLYQQN+IRDALVAG+ +IFH+H+DRV MANIAQ +NVLQA++LTEGE Sbjct: 301 WFDVEPGTNPGFLYQQNTIRDALVAGVHFHIFHEHNDRVHMANIAQMVNVLQAMVLTEGE 360 Query: 372 KMLLTPTYHVFNMYKVHQDAELLDLTLDSGSYSFDGKEIPEVSASASVTAEGVIHVSLCN 431 +MLLTPTYHVFNMYKVHQDA L++ D G+Y F E+P V+ SAS EG IHVS+C+ Sbjct: 361 QMLLTPTYHVFNMYKVHQDATRLEVNADVGTYKFGDDELPAVTVSASKDNEGAIHVSVCH 420 Query: 432 LNHASAAVLPLELRGL--AGQAVDIAGTTLAGATIDAHNSFSEPEAVVPQPFTAFKLEGD 489 L+ L L+L GL + A ++G L ++AHN+F P V P P+ + G Sbjct: 421 LDPHRDTDLTLKLNGLNKSLSASSVSGELLTANELNAHNTFERPNEVEPVPYEPASVSGH 480 Query: 490 TVTVELPAMSVTVLEITP 507 + +++PA SV + I P Sbjct: 481 EMELKVPASSVLRVTIRP 498 >BS_xsa [G] COG3534 Alpha-L-arabinofuranosidase Length = 148 Score = 675 bits (1741), Expect = 0.0, Method: Compositional matrix adjust. Identities = 0/492 (0%), Positives = 0/492 (0%), Gaps = 4/492 (0%) Query: 14 QRVVLHTDIRKGTINRNIYGHFSEHLGRCIYEGIWVGEDSPIPNTKGIRNDVVEALKEIK 73 L K T+ R + + + + V L + Sbjct: 4 NNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLIDMGEEIGLATVYRVLNQFD 63 Query: 74 VPVLRWPGGCFADEYHWKDGIGAREGRKRMINTHWGGTVENNHFGTHEFMELCAMLECEP 133 + + ++ + G V + E + + Sbjct: 64 DAGIVTRHNFEGGKSVFELTQQ---HHHDHLICLDCGKVIEFSDDSIEARQREIAAKHGI 120 Query: 134 YINGNVGSGTVQEMSEWVEYLTFNGVSPMAELRAKNGREEAWAVKYFGVGNENWGCGGNM 193 Sbjct: 121 180 Query: 194 RPEYYADLYRQYQTYVRNYGDNRIHRIACGPNADDYQWMEVLMREATRFMDSITLHYYTV 253 Sbjct: 181 240 Query: 254 PGATWEHKGAATGFDTAEYFTTLEKALRMEELVTRHSVIMDKYDPEKRVGLIVDEWGTWY 313 Sbjct: 241 300 Query: 314 DVEPGTNPGFLYQQNSIRDALVAGLTLNIFHKHSDRVRMANIAQTINVLQAVILTEGEKM 373 Sbjct: 301 360 Query: 374 LLTPTYHVFNMYKVHQDAELLDLTLDSGSYSFDGKEIPEVSASASVTAEGVIHVSLCNLN 433 Sbjct: 361 420 Query: 434 HASAAVLPLELRGLAGQAVDIAGTTLAGATIDAHNSFSEPEAVVPQPFTAFKLEGDTVTV 493 Sbjct: 421 - 479 Query: 494 ELPAMSVTVLEI 505 Sbjct: 480 491 >BS_abfA [G] COG3534 Alpha-L-arabinofuranosidase Length = 500 Score = 164 bits (414), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 135/516 (26%), Positives = 229/516 (44%), Gaps = 41/516 (7%) Query: 15 RVVLHTDIRKGTINRNIYGHFSEHLGRCIYEGIWVGEDSPIPNTKGIRNDVVEALKEIKV 74 R+++ + + G +++ IYG F EH+GR +YEGI+ D P + G R DV +KE++V Sbjct: 5 RMIVDKEYKIGEVDKRIYGSFIEHMGRAVYEGIY-EPDHPEADEDGFRKDVQSLIKELQV 63 Query: 75 PVLRWPGGCFADEYHWKDGIGAREGRKRMINTHWGGTVENNHFGTHEFMELCAMLECEPY 134 P++R+PGG F Y+W+DG+G E R R ++ W T E N GT+EF+ + E Sbjct: 64 PIIRYPGGNFLSGYNWEDGVGPVENRPRRLDLAW-QTTETNEVGTNEFLSWPKKVNTEVN 122 Query: 135 INGNVGSGTVQEMSEWVEYLTFNGVSPMAELRAKNGREEAWAVKYFGVGNE---NWGCGG 191 + N+G+ + VEY S ++LR +G E+ + +K + +GNE W G Sbjct: 123 MAVNLGTRGIDAARNLVEYCNHPKGSYWSDLRRSHGYEQPYGIKTWCLGNEMDGPWQIGH 182 Query: 192 NMRPEYYADLYRQYQTYVRNYGDNRIHRIACGPNADDY----QWMEVLMREATRFMDSIT 247 EY V + D I +ACG + W ++ +D I+ Sbjct: 183 KTADEY--GRLAAETAKVMKWVDPSIELVACGSSNSGMPTFIDWEAKVLEHTYEHVDYIS 240 Query: 248 LHYYTVPGATWEHKGAATGFDTAEYFTTLEKALRMEELVTRHSVIMD----KYDPEKRVG 303 LH Y G L +++ ++ + + D K +K + Sbjct: 241 LHTY-------------YGNRDNNLPNYLARSMDLDHFIKSVAATCDYVKAKTRSKKTIN 287 Query: 304 LIVDEWGTWY-------DVEPG-TNPGFLYQQNSIRDALVAGLTLNIFHKHSDRVRMANI 355 L +DEW WY VEP T L + DAL+ G L +H+DRV++A + Sbjct: 288 LSLDEWNVWYHSNEADKKVEPWITARPILEDIYNFEDALLVGSLLITMLQHADRVKIACL 347 Query: 356 AQTINVLQAVILTEGEKMLLTPTYHVFNMYKVHQDAELLDLTLDSGSYSF-DGKEIPEVS 414 AQ +NV+ ++ +G + P ++ + V+ E L + S Y D ++P V Sbjct: 348 AQLVNVIAPIMTEKGGEAWRQPIFYPYMHASVYGRGESLKPLISSPKYDCSDFTDVPYVD 407 Query: 415 ASASVTAEGVIHVSLCNLNHASAAV-LPLELRGLAGQAVDIAGTTLAGATIDAHNSFSEP 473 A A V +E +++ +N A + + LRG + L I A N + Sbjct: 408 A-AVVYSEEEETLTIFAVNKAEDQMETEISLRGFESYQI-AEHIVLEHQDIKATNQHNR- 464 Query: 474 EAVVPQPFTAFKLEGDTVTVELPAMSVTVLEITPKA 509 + VVP + + + +T +S V+ + ++ Sbjct: 465 KNVVPHSNGSSSVSENGLTAHFTPLSWNVIRLKKQS 500 >BH1861 [G] COG3534 Alpha-L-arabinofuranosidase Length = 500 Score = 156 bits (395), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 139/521 (26%), Positives = 226/521 (43%), Gaps = 43/521 (8%) Query: 10 IEMAQRVVLHTDIRKGTINRNIYGHFSEHLGRCIYEGIWVGEDSPIPNTKGIRNDVVEAL 69 + + +V+ + G I++ IYG F EHLGR +YEGI+ P + +G R DV+ + Sbjct: 1 MTLTATMVVDKSFKIGEIDKRIYGSFIEHLGRAVYEGIY-EPGHPDGDEQGFRKDVIRLV 59 Query: 70 KEIKVPVLRWPGGCFADEYHWKDGIGAREGRKRMINTHWGGTVENNHFGTHEFMELCAML 129 +E++VP++R+PGG F Y+W+DG+G R + ++ W T E N GT+EF++ + Sbjct: 60 QELQVPLVRYPGGNFVSGYNWEDGVGPVSERPKRLDLAW-RTTETNEIGTNEFVDWAKKV 118 Query: 130 ECEPYINGNVGSGTVQEMSEWVEYLTFNGVSPMAELRAKNGREEAWAVKYFGVGNE---N 186 E + N+GS V VEY S ++LR +G ++ +K + +GNE Sbjct: 119 GAEVNMAVNLGSRGVDAARNLVEYCNHPSGSYWSDLRISHGYKDPHNIKTWCLGNEMDGP 178 Query: 187 WGCGGNMRPEYYADLYRQYQTYVRNYGDNRIHRIACGPN----ADDYQWMEVLMREATRF 242 W G EY V D I +ACG + A W ++ + Sbjct: 179 WQIGQKTAEEY--GRVAAEAGKVMKLVDPSIELVACGSSNSKMATFADWEATVLDHTYDY 236 Query: 243 MDSITLHYYTVPGATWEHKGAATGFDTAEYFTTLEKALRMEELVTRHSVIMDKYDPEKR- 301 +D I+LH Y D A Y L +++ M+E + I D +KR Sbjct: 237 VDYISLHTYY----------GNRDDDLANY---LAQSMDMDEFIRSVIAIADYVKAKKRS 283 Query: 302 ---VGLIVDEWGTWY-------DVEP-GTNPGFLYQQNSIRDALVAGLTLNIFHKHSDRV 350 + L DEW W+ + P P L + DAL+ G L KH+DRV Sbjct: 284 KKTIHLSFDEWNVWFHSNEADRQITPWSVAPPLLEDIYTFEDALLVGSMLITLLKHADRV 343 Query: 351 RMANIAQTINVLQAVILTEGEKMLLTPTYHVFNMYKVHQDAELLDLTLDSGSY-SFDGKE 409 ++A +AQ +NV+ ++ +G ++ + V+ L + S Y S D + Sbjct: 344 KIACLAQLVNVIAPIMTEKGGPAWKQTIFYPYMHASVYGRGVALQAQISSPKYDSKDFTD 403 Query: 410 IPEVSASASVTAEGVIHVSLCNLNHASAAVLPLE--LRGLAGQAVDIAGTTLAGATIDAH 467 +P + A A V E V++ +N L L+ +R G V + L + A Sbjct: 404 VPYLDA-AVVHLEEAEEVTIFAVNKHQTESLNLQCDMRSFEGYHV-LEHIVLEHENMKAT 461 Query: 468 NSFSEPEAVVPQPFTAFKLEGDTVTVELPAMSVTVLEITPK 508 N E V P ++ +T L +S V+ + K Sbjct: 462 NQGR--EQVTPHHNGDSAIDQGRLTANLAKLSWNVIRLGKK 500 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 40,743,840 Number of extensions: 1736849 Number of successful extensions: 4592 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 4581 Number of HSP's successfully gapped: 4 Length of query: 509 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 398 Effective length of database: 25,576,357 Effective search space: 10179390086 Effective search space used: 10179390086 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)