BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0054.p (847 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value VNG1557G [H] COG1010 Precorrin-3B methylase 225 2e-58 sll0037 [S] COG2138 Uncharacterized conserved protein 177 9e-44 VNG1561Cm_1 [S] COG2138 Uncharacterized conserved protein 157 7e-38 RSp0623_1 [S] COG2138 Uncharacterized conserved protein 151 5e-36 VNG1555G [H] COG1010 Precorrin-3B methylase 120 1e-26 >VNG1557G [H] COG1010 Precorrin-3B methylase Length = 335 Score = 225 bits (574), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 122/255 (47%), Positives = 167/255 (65%), Gaps = 7/255 (2%) Query: 5 GKLLIIGFGPGRLEHITGRALAALEESEAVIGYTTYVDLIKPLLRQ--QEIVGTGMTEEV 62 G L+ +G GPGR E +T RA AAL ++E ++GYTTYVDL+ + +EI T M EV Sbjct: 81 GSLVAVGLGPGRPEGMTDRATAALADAEHIVGYTTYVDLLPDEITDDAEEIYDTPMCGEV 140 Query: 63 SRAQEAVRRAEAGQTVAVISSGDAGVYGMAGLVYEVLIERGWDRVTGVEVEVIPGVSAIQ 122 SR +EAV RA AG VA+I SGD VY +AGL E+ +G + ++ EV+PGV A Q Sbjct: 141 SRTEEAVDRALAGNHVAIIGSGDPNVYALAGLALEITESKGA-TPSMLDFEVVPGVPAAQ 199 Query: 123 SCSSLLGAPIMHDSCTISLSDHLTPWESIAARVEAAGAADFVIAFYNPRSGKRTRQIEEA 182 SC + LGAP+++DS +ISLSDHLTP I +R+ A + +F I+ YNP S KR ++A Sbjct: 200 SCGARLGAPLVNDSVSISLSDHLTPMPEIESRLHAVASEEFTISIYNPWSRKRRENFQKA 259 Query: 183 RQILLRYRDPSTPVGIVKSAYRDRQQTIVTTLQDMLDHE----IGMLSTVIVGNSATMVY 238 +ILL +RD TPVGIV A R+ ++ +TTL ++ + I M +TV++GN+ T V+ Sbjct: 260 CEILLAHRDADTPVGIVHGAGREDEEVEITTLGELESYGETDLIDMTTTVVIGNAETYVF 319 Query: 239 DGLMVTPRGYERKYS 253 D MVTPRGYE KY Sbjct: 320 DDRMVTPRGYETKYD 334 >sll0037 [S] COG2138 Uncharacterized conserved protein Length = 336 Score = 177 bits (448), Expect = 9e-44, Method: Compositional matrix adjust. Identities = 101/316 (31%), Positives = 159/316 (50%), Gaps = 57/316 (18%) Query: 442 VLLVGHGSRVEEGNEELRNFT---RALAARRPELAIETCFIELSAPSISEGIDKCVLGRA 498 +L++GHG+R E+G + +F +AL RP + CF+EL+ P+I G+ +CV Sbjct: 27 LLMIGHGTRDEDGRQTFLDFVAQYQALDHSRP---VIPCFLELTEPNIQAGVQQCVDQGF 83 Query: 499 GTVYVVPIILFAAGHSKLDIPLALDQAKLKYPGVEFVYGRPIGVQERAVEILLDRIAGXX 558 + +PI+LFAA H+K D+ LD+++ +P + F YGR G+ +++ R+ Sbjct: 84 EEISALPILLFAARHNKFDVTNELDRSRQAHPQINFFYGRHFGITPAILDLWKARL---- 139 Query: 559 XXXXXXXXXXELSGEPLEETLLVERGSSGQQNTGGLETAAALSVGETASAAARDKPYSKV 618 + S + N G++ Sbjct: 140 -----------------------NQLDSPEANPQGIDR---------------------- 154 Query: 619 KDEDTIVLVMGRGGSDPDANSDLCKLARLLWERTPYKSVEACFIAITKPSLPAGLERCLA 678 +DT++L +GRG SDPDAN D+ K+AR+LWE + Y++VE CFI I+ P L G R Sbjct: 155 --QDTVLLFVGRGSSDPDANGDVYKMARMLWEGSGYQTVETCFIGISHPRLEEGFRRARL 212 Query: 679 LGARKIIVLPYLLFTGVLMKQFNETVSRFAAAHPEIEVEAGTCLGSHPLLSDMLLERIEE 738 ++IIVLPY LF G L+K+ A PEIE+++ + +G P L ++ ER E Sbjct: 213 YQPKRIIVLPYFLFMGALVKKIFTITEEQRATFPEIEIQSLSEMGIQPELLALVREREIE 272 Query: 739 TLAGRTAANCDNCMYR 754 T G+ A NC+ C +R Sbjct: 273 TQLGQVAMNCEACKFR 288 >VNG1561Cm_1 [S] COG2138 Uncharacterized conserved protein Length = 310 Score = 157 bits (397), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 104/316 (32%), Positives = 153/316 (48%), Gaps = 55/316 (17%) Query: 441 SVLLVGHGSRVEEGNEELRNFTRALAARRPELAIETCFIELSAPSISEGIDKCVLGRAGT 500 +VLLVGHGSR E NE++R+ L R + ++ F+EL+ P+I + ID A Sbjct: 5 AVLLVGHGSRREASNEQVRSLAADLEGRLG-VPVDAGFLELAEPTIPDAIDALAPAVA-N 62 Query: 501 VYVVPIILFAAGHSKLDIPLALDQAKLKYPGVEFVYGRPIGVQERAVEILLDRIAGXXXX 560 + VV + LFAA H K D+P AL +A+ ++P VEF G +GV V++L DR A Sbjct: 63 ISVVHLSLFAASHVKNDVPAALQRARSQHPEVEFNNGSHLGVHPAIVDLLDDRAAD---- 118 Query: 561 XXXXXXXXELSGEPLEETLLVERGSSGQQNTGGLETAAALSVGETASAAARDKPYSKVKD 620 +E L V+R + Sbjct: 119 --------------VEADLGVDRAA----------------------------------- 129 Query: 621 EDTIVLVMGRGGSDPDANSDLCKLARLLWERTPYKSVEACFIAITKPSLPAGLERCLALG 680 +D V++ RG SDPDAN+D+ KLARLL+E + V+A FI +T+P+L L Sbjct: 130 DDVAVVLCARGSSDPDANADVHKLARLLYEGRAFSRVDATFIGVTEPTLDETLHDVAKHR 189 Query: 681 ARKIIVLPYLLFTGVLMKQFNETVSRFAAAHPEIEVEAGTCLGSHPLLSDMLLERIEETL 740 ++V+PY+L GVL + E + F A +P +E G LG+ L D+L +R +E Sbjct: 190 PDAVVVVPYMLGDGVLTGRIREGAAEFDADYPYVEAACGDPLGTDSRLLDVLGDRWQEAR 249 Query: 741 AGRTAANCDNCMYREE 756 G +CD C Y+ E Sbjct: 250 TGSVEMSCDTCKYKVE 265 >RSp0623_1 [S] COG2138 Uncharacterized conserved protein Length = 269 Score = 151 bits (381), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 97/307 (31%), Positives = 143/307 (46%), Gaps = 53/307 (17%) Query: 441 SVLLVGHGSRVEEGNEELRNFTRALAARRPELAIETCFIELSAPSISEGIDKCVLGRAGT 500 +++L GHGSR +G E L R + F+E + P++ E +D V AGT Sbjct: 16 AIVLAGHGSRDPDGVAEFLALVALLRERAGGRTVAHGFLEFATPTLDEAVDAVVADGAGT 75 Query: 501 VYVVPIILFAAGHSKLDIPLALDQAKLKYPGVEFVYGRPIGVQERAVEILLDRIAGXXXX 560 V +VP +L AA H+K D+P L K +P V+F +G + + + + + +R Sbjct: 76 VVMVPGVLLAATHAKNDMPSELLALKQAHPQVDFRFGAAMDLHPKLLALCRER------- 128 Query: 561 XXXXXXXXELSGEPLEETLLVERGSSGQQNTGGLETAAALSVGETASAAARDKPYSKVKD 620 ++ G S Q V Sbjct: 129 ------------------IVEAEGRSAQT----------------------------VSR 142 Query: 621 EDTIVLVMGRGGSDPDANSDLCKLARLLWERTPYKSVEACFIAITKPSLPAGLERCLALG 680 DT ++V+GRG SDPDANS++ KL R+L E + + C+ +P + GL+ LG Sbjct: 143 ADTCLVVVGRGTSDPDANSEIAKLTRMLEEGMGFGTAFVCYAGTARPLVADGLKAAARLG 202 Query: 681 ARKIIVLPYLLFTGVLMKQFNETVSRFAAAHPEIEVEAGTCLGSHPLLSDMLLERIEETL 740 R+I+VLPY LF GVL+K+ + AA HPEIEV LG HP ++D+ LER E + Sbjct: 203 YRRIVVLPYFLFDGVLVKRIYGAANDLAARHPEIEVLRAPYLGVHPHVADVFLERAREGI 262 Query: 741 AGRTAAN 747 GR A N Sbjct: 263 EGRAAMN 269 Score = 59.3 bits (142), Expect = 3e-08, Method: Compositional matrix adjust. Identities = 38/136 (27%), Positives = 65/136 (47%), Gaps = 7/136 (5%) Query: 622 DTIVLVMGRGGSDPDANSDLCKLARLLWERTPYKSVEACFIAITKPSLPAGLERCLALGA 681 D +++ G G DPD ++ L LL ER ++V F+ P+L ++ +A GA Sbjct: 14 DYAIVLAGHGSRDPDGVAEFLALVALLRERAGGRTVAHGFLEFATPTLDEAVDAVVADGA 73 Query: 682 RKIIVLPYLLFTGVLMKQFNETVSRFAA---AHPEIEVEAGTCLGSHPLLSDMLLERIEE 738 ++++P +L K N+ S A AHP+++ G + HP L + ERI E Sbjct: 74 GTVVMVPGVLLAATHAK--NDMPSELLALKQAHPQVDFRFGAAMDLHPKLLALCRERIVE 131 Query: 739 T--LAGRTAANCDNCM 752 + +T + D C+ Sbjct: 132 AEGRSAQTVSRADTCL 147 >VNG1555G [H] COG1010 Precorrin-3B methylase Length = 276 Score = 120 bits (300), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 87/267 (32%), Positives = 132/267 (49%), Gaps = 32/267 (11%) Query: 5 GKLLIIGFGPGRLEHITGRALAALEESEAVIGYTTYVDLIK-----PLLR-----QQEIV 54 G L ++G GPG +T RA + ++ V+ Y + ++ P QE+V Sbjct: 11 GTLYVVGIGPGLPGGMTQRAKDVVRTADCVVASNLYQEFLRRDGTLPPADAGERPDQEVV 70 Query: 55 GTGMTEEVSRAQEAVRRAEAGQTVAVISSGDAGVYGMAGLVYEVLIERGWDRVTGVEVEV 114 + M ++ A+EA R G+ VA +S GD VYG + LVY + E V +E+ Sbjct: 71 RSTMGRQIELAKEAFERVRDGEDVAHVSGGDPNVYGKSDLVYLMAEEEA---AYDVPIEI 127 Query: 115 IPGVSAIQSCSSLLGAPIMHDSCTISLSDHLTPWESIAARVEAAGAADFVIAFYNPRSGK 174 +PGV+A ++ LGAP+ +D CTISLSD WE I ++ AA + FV+ YN Sbjct: 128 VPGVTAALGGAANLGAPLSNDFCTISLSDKWRGWEEIEEKLRAAAISGFVVVLYNC---- 183 Query: 175 RTRQIEEARQILLRYRDPSTPVGIVKSAYR-------DRQQTIVTTLQDMLDH--EI-GM 224 R E A +L R PV I+ A R D + +TTL + DH E+ GM Sbjct: 184 -WRDYERAIGVLREERADDVPVAIINDAGRGDAGRNTDGETHTITTLGSVTDHSGEVGGM 242 Query: 225 LSTVIVGNSATMVYDG----LMVTPRG 247 +++++G T V++ +VTPRG Sbjct: 243 GASILIGTDETAVWENDHRKHLVTPRG 269 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.133 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 56,943,266 Number of extensions: 2339776 Number of successful extensions: 12794 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 12782 Number of HSP's successfully gapped: 31 Length of query: 847 Length of database: 38,378,431 Length adjustment: 115 Effective length of query: 732 Effective length of database: 25,115,021 Effective search space: 18384195372 Effective search space used: 18384195372 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 120 (50.8 bits)