BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0047.p (267 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PAE0590 [H] COG0007 Uroporphyrinogen-III methylase 134 2e-31 SMc01053_2 [H] COG0007 Uroporphyrinogen-III methylase 111 1e-24 AGc2683_2 [H] COG0007 Uroporphyrinogen-III methylase 110 3e-24 BMEI1768_2 [H] COG0007 Uroporphyrinogen-III methylase 109 5e-24 BS_nasF_1 [H] COG0007 Uroporphyrinogen-III methylase 108 9e-24 >PAE0590 [H] COG0007 Uroporphyrinogen-III methylase Length = 228 Score = 134 bits (337), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 23/219 (10%), Positives = 37/219 (16%), Gaps = 16/219 (7%) Query: 10 VYIVGAGPGDPELITLKGSRILRSADVVLYADSLVSGELIASVKPGA---EVLQSSGMAL 66 L S V D+ V Q A Sbjct: 4 AGRSNNQVRPVTLTRNYTKHAEGSVLVEFG-DTKVLCTASIEEGVPRFLKGQGQGWITAE 62 Query: 67 EQQVEIMTAAVKAGKS---VARIHTGDPSMYGAILEQMSLLKLNGVSYEIVPGVSSVFAS 123 + T A ++ T + A + ++ + I + A Sbjct: 63 YGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAVDLKALGEFTITLDCDVLQAD 122 Query: 124 AAALGAELTVPELTQTV-----ILTRAEGRTPVPEREQLRQLAAHHCTVALFLSASLAEH 178 T V + E + A V L Sbjct: 123 GG----TRTASITGACVALVDALQKLVENGKLKTNPMKGMVAAVSVGIVNGEAVCDLEYV 178 Query: 179 TASEFLAAGWSPDTPIAVVKRATWPDQQILRTTVARLAA 217 S T + + T L Sbjct: 179 EDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLIL 217 >SMc01053_2 [H] COG0007 Uroporphyrinogen-III methylase Length = 228 Score = 111 bits (278), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 23/221 (10%), Positives = 49/221 (22%), Gaps = 12/221 (5%) Query: 3 AAGLEPKVYIVGAGPGDPELITLKGSRILRSADVVLYADSLVSGELIASVKPGAEVL--- 59 + +GS ++ D + + E + G Sbjct: 2 RPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVL-CTASIEEGVPRFLKGQGQGWIT 60 Query: 60 QSSGMALEQQVEIMTAAVK---AGKSVARIHTGDPSMYGAILEQMSLLKLNGVSYEIVPG 116 GM G I A ++ +L + V Sbjct: 61 AEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAVDLKALGEFTITLDCDVLQ 120 Query: 117 VSSVFASAAALGAELTVPELTQTVILTRAEGRTP--VPEREQLRQLAAHHCTVALFLSAS 174 +A+ GA + + + Q ++ P A+ Sbjct: 121 ADGGTRTASITGACVALVDALQKLVENGKLKTNPMKGMV---AAVSVGIVNGEAVCDLEY 177 Query: 175 LAEHTASEFLAAGWSPDTPIAVVKRATWPDQQILRTTVARL 215 + + A + + D I V+ + + L Sbjct: 178 VEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLILL 218 >AGc2683_2 [H] COG0007 Uroporphyrinogen-III methylase Length = 228 Score = 110 bits (274), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 23/217 (10%), Positives = 49/217 (22%), Gaps = 12/217 (5%) Query: 7 EPKVYIVGAGPGDPELITLKGSRILRSADVVLYADSLVSGELIASVKPGAEVL---QSSG 63 + +GS ++ D + + E + G G Sbjct: 6 RSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVL-CTASIEEGVPRFLKGQGQGWITAEYG 64 Query: 64 MALEQQVEIMTAAVK---AGKSVARIHTGDPSMYGAILEQMSLLKLNGVSYEIVPGVSSV 120 M G I A ++ +L + V Sbjct: 65 MLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAVDLKALGEFTITLDCDVLQADGG 124 Query: 121 FASAAALGAELTVPELTQTVILTRAEGRTP--VPEREQLRQLAAHHCTVALFLSASLAEH 178 +A+ GA + + + Q ++ P A+ + + Sbjct: 125 TRTASITGACVALVDALQKLVENGKLKTNPMKGMV---AAVSVGIVNGEAVCDLEYVEDS 181 Query: 179 TASEFLAAGWSPDTPIAVVKRATWPDQQILRTTVARL 215 A + + D I V+ + + L Sbjct: 182 AAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLILL 218 >BMEI1768_2 [H] COG0007 Uroporphyrinogen-III methylase Length = 228 Score = 109 bits (272), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 0/244 (0%), Positives = 0/244 (0%), Gaps = 12/244 (4%) Query: 10 VYIVGAGPGDPELITLKGSRILRSADVVLYADSLVSGELIASVKPGAEVL---QSSGMAL 66 ++ I P Sbjct: 5 GRSNNQVRPVTLTRNYTKHAEGSVLVEFGD-TKVLCTASIEEGVPRFLKGQGQGWITAEY 63 Query: 67 EQQVEIMTAAVK---AGKSVARIHTGDPSMYGAILEQMSLLKLNGVSYEIVPGVSSVFAS 123 A + L LK G + Sbjct: 64 GMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAVDLKALGEFTITLDCDVLQADG 123 Query: 124 AAALGAELTVPELTQTVILTRAEGRTP--VPEREQLRQLAAHHCTVALFLSASLAEHTAS 181 + + E P +AA + + E+ Sbjct: 124 GTRTASITGACVALVDALQKLVENGKLKTNP---MKGMVAAVSVGIVNGEAVCDLEYVED 180 Query: 182 EFLAAGWSPDTPIAVVKRATWPDQQILRTTVARLAADLREAGITMHAMILAGWALDPALT 241 Sbjct: 181 240 Query: 242 DRDQ 245 Sbjct: 241 244 >BS_nasF_1 [H] COG0007 Uroporphyrinogen-III methylase Length = 228 Score = 108 bits (270), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 0/234 (0%), Positives = 0/234 (0%), Gaps = 14/234 (5%) Query: 10 VYIVGAGPGDPELITLKGSRILRSADVVLYADSLVSGELIASVKPGAEVLQSSGMA---L 66 V +L S E + L Sbjct: 8 NNQVRPVTLTRNYTKHAEGSVLVEFGDTKV-LCTASIEEGVPRFLKGQGQGWITAEYGML 66 Query: 67 EQQVEIMTAAVKA---GKSVARIHTGDPSMYGAILEQMSLLKLNGVSYEIVPGVSSVFAS 123 + A A + + L ++ + + Sbjct: 67 PRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAVDLKALGEFTITLDCDVLQADGGTR 126 Query: 124 AAALGAELTVPELTQTVILTRAEGR----TPVPEREQLRQLAAHHCTVALFLSASLAEHT 179 A++ ++ + + + + + L Sbjct: 127 TASITGACVALVDALQKLVENGKLKTNPMKGMVAAVSVGIVNG---EAVCDLEYVEDSAA 183 Query: 180 ASEFLAAGWSPDTPIAVVKRATWPDQQILRTTVARLAADLREAGITMHAMILAG 233 Sbjct: 184 237 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.131 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,453,640 Number of extensions: 696003 Number of successful extensions: 3517 Number of sequences better than 1.0e-05: 42 Number of HSP's gapped: 3443 Number of HSP's successfully gapped: 42 Length of query: 267 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 162 Effective length of database: 26,268,361 Effective search space: 4255474482 Effective search space used: 4255474482 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)