BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0045.p (574 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA3626 [H] COG1797 Cobyrinic acid a,c-diamide synthase 169 2e-41 MK1643 [H] COG1797 Cobyrinic acid a,c-diamide synthase 157 5e-38 MA0106 [H] COG1797 Cobyrinic acid a,c-diamide synthase 153 8e-37 MK1573 [H] COG1797 Cobyrinic acid a,c-diamide synthase 119 1e-26 >MA3626 [H] COG1797 Cobyrinic acid a,c-diamide synthase Length = 497 Score = 169 bits (427), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 92/227 (40%), Positives = 129/227 (56%), Gaps = 9/227 (3%) Query: 46 VQAFKCGPDYIDPAYHAAVTGRTARNLDSWMTSGGYMLEYFLRSSE---GADLSVIEGVM 102 VQ FK DYIDP+YH +TGR RNLD ++ +L+ + + E GAD+++IEGV Sbjct: 46 VQPFKVALDYIDPSYHTEITGRFCRNLDGYLMDENGILDVYSHACETGSGADIAIIEGVR 105 Query: 103 GLYDGKEATGLTGSTAEIALLTGSPVLLVVDVRSMGRSAAAIVLGFQQLEPDVRIAAVIV 162 GLY+G E GSTA+IA + PV+ V++ RS+ RSAAA++ G++ +PDV IA VI+ Sbjct: 106 GLYEGFEGLSDLGSTAQIAKILKCPVVFVINARSITRSAAALISGYKNFDPDVEIAGVIL 165 Query: 163 NRCGSASHYRTVKAAIEAACGIPVIGWLARDQGLDIPERHLGLLPAVE------RGELEP 216 N G H + K AIE G+PVIG + RD + I RHLGL+PA+E G E Sbjct: 166 NNIGGRRHAQKAKEAIEHYTGVPVIGIIPRDPSMQISMRHLGLMPALEGRRRLGDGGFED 225 Query: 217 LFDRAADLLEAGTDLEVLLQIAAAASVLRYPAAGKILPEEGPAEELP 263 +++ G D++ L+IA +A L P P G P Sbjct: 226 RLRGIEEIINKGIDVDRFLEIAGSAKSLTSPENSIFSPAAGAGSPRP 272 Score = 111 bits (277), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 78/245 (31%), Positives = 109/245 (44%), Gaps = 17/245 (6%) Query: 336 LTYPPLDRSAPQALASVYDPAPVIAVAQDAAFNFYYADNXXXXXXXXXXXXPFSPLSGEG 395 LT P +P A A P P I +A D AFNFYY DN FSP++ Sbjct: 253 LTSPENSIFSPAAGAG--SPRPRIGIALDEAFNFYYRDNIDLLELAGAEIVYFSPVNDPE 310 Query: 396 IPPEADGIYLGGGFPEEFAATIAGNSXXXXXXXXXXXXXXXXXXECGGYMVLVRSLTDRA 455 + P+ DG+Y+GGG+PE FAA + N ECGG M L ++ Sbjct: 311 L-PDVDGLYIGGGYPELFAAELEANESMRRSIKEASAAGMPIYAECGGLMYLTEKISTGV 369 Query: 456 ---GAVHE---------MAGIIPAHSVMQDRRTALGYREVTARADCLLLKQGERLRGHEF 503 G H+ M G +P H++M R + Y T DCL+ K+G +GHEF Sbjct: 370 PGKGTYHDASMPESTYIMVGALPGHTIMGQTRV-VSYNIGTLDRDCLIGKEGNSFKGHEF 428 Query: 504 HYSQMSYSPGEPQLYAYDSRGRS-GSQPEGYARGNIMAAYAHIHLASHIPAAIRLVEACR 562 H+S++ P + RG +G GN + +YAH+H ++ A LVEA Sbjct: 429 HHSEIREIPEYAEFAIALLRGTGIKGDRDGLIVGNTLGSYAHLHGVAYRELAGSLVEAAG 488 Query: 563 SYHRS 567 + S Sbjct: 489 KFRAS 493 >MK1643 [H] COG1797 Cobyrinic acid a,c-diamide synthase Length = 463 Score = 157 bits (396), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 93/230 (40%), Positives = 136/230 (59%), Gaps = 14/230 (6%) Query: 46 VQAFKCGPDYIDPAYHAAVTGRTARNLDSWMTSGGYMLEYFLRSSEGADLSVIEGVMGLY 105 VQ FK GPDYIDP+YH +GR NLD+++ ++ F EGADL+V+EGV GLY Sbjct: 44 VQPFKVGPDYIDPSYHWLASGRPCGNLDTFLFREKHVRWLFEHRCEGADLAVVEGVRGLY 103 Query: 106 DGKEATGLTGSTAEIALLTGSPVLLVVDVRSMGRSAAAIVLGFQQLEPDVRIAAVIVNRC 165 +G A G+ GST ++ + +PV+L+VD RS+ +S AA+V G+ +LE IA VI+NR Sbjct: 104 EGIGAVGVRGSTYHVSEVLNAPVVLIVDARSLTKSVAALVKGYAELE-GANIAGVILNRI 162 Query: 166 GSASHYRTVKAAIEAACGIPVIGWLARDQGLDIPERHLGLLPAVERGELEPLFDR---AA 222 S HY V+ A+ + V+G++ RD+ L + RHLGL+P ER LE + +R A Sbjct: 163 RSEVHYHKVRRALVKYTDVKVLGYVPRDRRLKVEYRHLGLVPTPER--LEEMRERLRTVA 220 Query: 223 DLLEAGTDLEVLLQIAAAASVLRYPAAGKILPEEGPAEELPSEAVPAARK 272 +++ DL+ L+ +A AAG + P E P E P++ A K Sbjct: 221 EVISEHVDLDALIDVAE--------AAGPLGPGERPWEVNPTKCRIAVAK 262 Score = 116 bits (290), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 69/199 (34%), Positives = 92/199 (46%), Gaps = 13/199 (6%) Query: 359 IAVAQDAAFNFYYADNXXXXXXXXXXXXPFSPLSGEGIPPEADGIYLGGGFPEEFAATIA 418 IAVA+D AFNFYY +N FSP+ E +PP+AD +Y+GGG+PE FA + Sbjct: 258 IAVAKDEAFNFYYPENLEALEENGAKLLEFSPVRDEDVPPDADALYIGGGYPELFARQLE 317 Query: 419 GNSXXXXXXXXXXXXXXXXXXECGGYMVLVRSL---TDRAGAVHEMAGIIPAHSVMQDRR 475 ECGG+M L R L DR + G+ M DR Sbjct: 318 DAESTRNSIRELAESGAPIYAECGGFMYLCRELRWNEDR----YRWVGVFDVAVEMTDRV 373 Query: 476 TALGYREVTARADCLLLKQGERLRGHEFHYSQMSYSPGEPQLY----AYDSRGRSGSQPE 531 L Y A D + ++GE +GHEFHYS++ G Y RGR G +P+ Sbjct: 374 QGLSYTVARAVDDTPVTRKGETFKGHEFHYSRLVRPEGLESAYRIIRGQGWRGREGFRPK 433 Query: 532 GYARGNIMAAYAHIHLASH 550 N++ Y H+H ASH Sbjct: 434 DLP--NVLGTYVHVHAASH 450 >MA0106 [H] COG1797 Cobyrinic acid a,c-diamide synthase Length = 458 Score = 153 bits (386), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 82/200 (41%), Positives = 128/200 (64%), Gaps = 10/200 (5%) Query: 46 VQAFKCGPDYIDPAYHAAVTGRTARNLDSWMTSGGYMLEYFLRSSEGADLSVIEGVMGLY 105 VQ +K GPDYIDP++H A+ GR++RNLD++M + + R+S AD++V+EGVMGL+ Sbjct: 53 VQPYKVGPDYIDPSHHTAICGRSSRNLDTYMMGTEGVRQTVARTSADADIAVVEGVMGLF 112 Query: 106 DGKEATGLTGSTAEIALLTGSPVLLVVDVRSMGRSAAAIVLGFQQLEPDVRIAAVIVNRC 165 DG ++T + S+A +A PV+LV++V M RS AAI+ G+ + +P+VRIA VI+N+ Sbjct: 113 DGIDSTEI-ASSAHVAKTLDIPVILVINVHGMSRSTAAILKGYSEFDPEVRIAGVILNQV 171 Query: 166 GSASHYRTVKAAIEAACGIPVIGWLARDQGLDIPERHLGLLPAVERGELEPLFDRA--AD 223 GS H V ++ IPV+G + R + +++P RHLGL A E+ ++ A A Sbjct: 172 GSPRHVELVVNSLPG--NIPVVGTIPRRKDIEVPSRHLGLYMAHEKD-----YNTAEMAA 224 Query: 224 LLEAGTDLEVLLQIAAAASV 243 +E DL+ +L++A SV Sbjct: 225 FIEENVDLDAVLELAEPCSV 244 Score = 89.7 bits (221), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 71/212 (33%), Positives = 94/212 (44%), Gaps = 17/212 (8%) Query: 359 IAVAQDAAFNFYYADNXXXXXXXXXXXXPFSPLSGEGIPPEADGIYLGGGFPEEFAATIA 418 I VA D AF FYY D FSP+ GE P+ DGIY GGG+PE +A + Sbjct: 259 IGVAWDPAFCFYYRDMFDAFRDHGAEVVFFSPMEGE--LPDVDGIYFGGGYPELYAEVLE 316 Query: 419 GNSXXXXXXXXXXXXXXXXXXECGGYMVLVRS--LTDRAGAVHEMAGIIPAHSVMQDRRT 476 NS ECGG + L + + DR +++A ++PA++ M +R Sbjct: 317 -NSETTRKLKGLAADGLPIYAECGGLLYLCGTYEVDDRT---YKLADVVPANTRMTNRLK 372 Query: 477 ALGYREVTARADCLLLKQGERLRGHEFHYSQMSYSPGEPQLYAYDSRGRSGSQP--EGYA 534 ALGY E L +RGHEFHYS +AY+ G Q +G Sbjct: 373 ALGYTEARPLDRNL---SSRNIRGHEFHYSLTECD--RDARFAYEMIRGKGIQNGFDGLL 427 Query: 535 RGNIMAAYAHIHLASHIPAAIRLVEACRSYHR 566 N +A Y H H AS P + V CR Y + Sbjct: 428 EHNTLAGYMHSHPAS-FPVD-KFVGKCREYKK 457 >MK1573 [H] COG1797 Cobyrinic acid a,c-diamide synthase Length = 211 Score = 119 bits (298), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 0/196 (0%), Positives = 0/196 (0%), Gaps = 4/196 (2%) Query: 46 VQAFKCGPDYIDPAYHAAVTGRTARNLDSWMTSGGYMLEYFLRSSEGADLSVIEGVMGLY 105 + A D + + A + + + Sbjct: 33 LIAVLLSAQATDVSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKT 92 Query: 106 DGKEATGLTGSTAEIALLTGSPVLLVVDVRSMGRSAAAIVLGFQQLEPDV--RIAAVIVN 163 G E + + ++ + A Sbjct: 93 CRILLEQHNGEVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAP 152 Query: 164 RCGSASHYRTVKAAIEAAC-GIPVIGWLARDQGLDIPERHLGLLPAVERGEL-EPLFDRA 221 + + A + + I + +E Sbjct: 153 GKNVEQVEEKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEY 212 Query: 222 ADLLEAGTDLEVLLQI 237 Sbjct: 213 228 Score = 82.4 bits (202), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 0/201 (0%), Positives = 0/201 (0%), Gaps = 25/201 (12%) Query: 359 IAVAQDAAFNFYYADNXXXXXXXXXXXXPFSPLSGEGIP--------PEADGIYLGGGFP 410 Sbjct: 250 ----------- 298 Query: 411 EEFAATIAGNSXXXXXXXXXXXXXXXXXXECGGYMVLVRSLTDRAGAVHEMAGIIPAHSV 470 Sbjct: 299 -- 356 Query: 471 MQDRRTALGYREVTARADCLLLKQGERLRGHEFHYSQMSYSPGEPQLYAYD-SRGRSGSQ 529 Sbjct: 357 -- 414 Query: 530 P-EGYARGNIMAAYAHIHLAS 549 Sbjct: 415 435 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 38,731,941 Number of extensions: 1553382 Number of successful extensions: 3621 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 3602 Number of HSP's successfully gapped: 8 Length of query: 574 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 462 Effective length of database: 25,461,023 Effective search space: 11762992626 Effective search space used: 11762992626 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 119 (50.4 bits)