BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0044.p (221 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ydhR [KG] COG1940 Transcriptional regulator/sugar kinase 233 2e-61 lin0809 [KG] COG1940 Transcriptional regulator/sugar kinase 224 9e-59 CAC1523 [KG] COG1940 Transcriptional regulator/sugar kinase 223 1e-58 SP1721 [KG] COG1940 Transcriptional regulator/sugar kinase 166 2e-41 SPy1811 [KG] COG1940 Transcriptional regulator/sugar kinase 166 2e-41 >BS_ydhR [KG] COG1940 Transcriptional regulator/sugar kinase Length = 299 Score = 233 bits (593), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 110/190 (57%), Positives = 133/190 (70%) Query: 3 VLGAIEAGGTKFVCGIGNEDGTIIDRVSFPTTTPEETMGLVLDYFTGKNVEAIGIGSFGP 62 +LG IEAGGTKFVC +G EDGTIIDR+ FPT P+ET+ V+ YF+ +++AIGIGSFGP Sbjct: 1 MLGGIEAGGTKFVCAVGREDGTIIDRIEFPTKMPDETIEKVIQYFSQFSLQAIGIGSFGP 60 Query: 63 IDPVIGSPTYGYITTTPKPHWGQYNLVGTVAGHFSVPVGFDTDVNGAALGEYKWGAAQGL 122 +D S TYG IT TPK W Y + TV +PVGF TDVN AALGE+ +G A+GL Sbjct: 61 VDNDKTSQTYGTITATPKAGWRHYPFLQTVKNEMKIPVGFSTDVNAAALGEFLFGEAKGL 120 Query: 123 DSCLYXXXXXXXXXXXXXXXKLVHGLSHPEMGHILVRRHPEDKFEGFCPYHSDCLEGLAA 182 DSCLY +L+ GLSHPEMGHI +RRHP+D ++G CPYH DC EGLA+ Sbjct: 121 DSCLYITIGTGIGAGAIVEGRLLQGLSHPEMGHIYIRRHPDDVYQGKCPYHGDCFEGLAS 180 Query: 183 GPAIGKRWGK 192 GPAI RWGK Sbjct: 181 GPAIEARWGK 190 >lin0809 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 290 Score = 224 bits (571), Expect = 9e-59, Method: Compositional matrix adjust. Identities = 112/192 (58%), Positives = 129/192 (67%), Gaps = 2/192 (1%) Query: 3 VLGAIEAGGTKFVCGIGNEDGTIIDRVSFPTTTPEETMGLVLDYFTG--KNVEAIGIGSF 60 V GAIEAGGTKFV IG + G II R S+PTT P ETM V+ +F ++AIGIGSF Sbjct: 2 VYGAIEAGGTKFVVAIGEKSGKIIKRESYPTTEPAETMKAVIQFFKQYQDELKAIGIGSF 61 Query: 61 GPIDPVIGSPTYGYITTTPKPHWGQYNLVGTVAGHFSVPVGFDTDVNGAALGEYKWGAAQ 120 GPID S TYGYIT TPK W Y++VG + F+VP+GF TDVN AALGE GAA Sbjct: 62 GPIDIQKSSATYGYITQTPKLAWRNYDIVGAMKKEFNVPIGFTTDVNAAALGEVSLGAAA 121 Query: 121 GLDSCLYXXXXXXXXXXXXXXXKLVHGLSHPEMGHILVRRHPEDKFEGFCPYHSDCLEGL 180 GLDSC+Y K++ G SHPEMGHI+VRRH D+F G CP HSDCLEGL Sbjct: 122 GLDSCIYLTIGTGIGGGAVVSGKILEGFSHPEMGHIMVRRHKRDRFTGSCPSHSDCLEGL 181 Query: 181 AAGPAIGKRWGK 192 AAG AI KRWG+ Sbjct: 182 AAGGAIEKRWGQ 193 >CAC1523 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 288 Score = 223 bits (569), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 109/188 (57%), Positives = 128/188 (68%) Query: 5 GAIEAGGTKFVCGIGNEDGTIIDRVSFPTTTPEETMGLVLDYFTGKNVEAIGIGSFGPID 64 GAIEAGGTKFVC + +E+ II+R S TT PEETM LV +F +EAIGIGSFGPID Sbjct: 4 GAIEAGGTKFVCAVSDENLNIIERESIKTTIPEETMTLVFKFFDKFKLEAIGIGSFGPID 63 Query: 65 PVIGSPTYGYITTTPKPHWGQYNLVGTVAGHFSVPVGFDTDVNGAALGEYKWGAAQGLDS 124 S TYGYIT TPK W Y+ VG V +++P+G+ TDVN AALGE K GAA L+S Sbjct: 64 VNKKSKTYGYITNTPKQGWANYDFVGAVKNRYNIPIGWTTDVNAAALGELKKGAAVNLES 123 Query: 125 CLYXXXXXXXXXXXXXXXKLVHGLSHPEMGHILVRRHPEDKFEGFCPYHSDCLEGLAAGP 184 C+Y KL+ G HPEMGHILVRRH +D +EG CP+H DCLEG+AAGP Sbjct: 124 CVYLTVGTGIGGGAVVKGKLLEGYGHPEMGHILVRRHEKDTYEGKCPFHKDCLEGMAAGP 183 Query: 185 AIGKRWGK 192 AI RWGK Sbjct: 184 AIEARWGK 191 >SP1721 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 295 Score = 166 bits (420), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 83/196 (42%), Positives = 122/196 (62%), Gaps = 6/196 (3%) Query: 2 KVLGAIEAGGTKFVCGIGNEDGTIIDRVSFPTTTPEETMGLVLDYFTG-KNVEAIGIGSF 60 K+ G++EAGGTKFVC +G+E+ ++++ FPTTTP ET+ +++F+ N+ + +GSF Sbjct: 3 KLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKTIEFFSKFDNLAGLAVGSF 62 Query: 61 GPIDPVIGSPTYGYITTTPKPHWGQYNLVGTVAGHFSVPVGFDTDVNGAALGEY--KWGA 118 GPID S TYG+ITTTPKP+W +L+G + +VP+ F TDVN +A GE + A Sbjct: 63 GPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPMYFTTDVNSSAYGEMVARNNA 122 Query: 119 AQGLDSCLYXXXXXXXXXXXXXXXKLVHGLSHPEMGHILVRRHP---EDKFEGFCPYHSD 175 +++ +Y + + G+ HPEMGH V RHP E +F+G CP+H Sbjct: 123 GGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHYYVARHPMDIEKEFKGVCPFHKG 182 Query: 176 CLEGLAAGPAIGKRWG 191 CLEG AAGP++ R G Sbjct: 183 CLEGYAAGPSLEARTG 198 >SPy1811 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 294 Score = 166 bits (420), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 88/195 (45%), Positives = 116/195 (59%), Gaps = 7/195 (3%) Query: 2 KVLGAIEAGGTKFVCGIGNEDGTIIDRVSFPTTTPEETMGLVLDYFTG--KNVEAIGIGS 59 K+ G+IEAGGTKFVC +G+E+ T++D+ FPTTTPEET+ + YF ++ + IGS Sbjct: 3 KLYGSIEAGGTKFVCAVGDEEFTVVDKTQFPTTTPEETIARTIAYFKAFEADLAGMAIGS 62 Query: 60 FGPIDPVIGSPTYGYITTTPKPHWGQYNLVGTVAGHFSVPVGFDTDVNGAALGEYKWGAA 119 FGPID S TYGYITTTPK W +L+G ++ F +P TDVN +A GE A Sbjct: 63 FGPIDIDPSSETYGYITTTPKSGWANVDLLGQLSAAFKIPFDVTTDVNSSAYGEVL--AR 120 Query: 120 QGLDSCLYXXXXXXXXXXXXXXXKLVHGLSHPEMGHILVRRHPEDKFEGF---CPYHSDC 176 G++S +Y + GL H E GH V HP+D +GF CP+H C Sbjct: 121 PGVESLVYYTIGTGIGAGAIQHGHFIGGLGHTEAGHTYVMPHPDDMAKGFLGVCPFHKGC 180 Query: 177 LEGLAAGPAIGKRWG 191 LEG+AAGP+I R G Sbjct: 181 LEGMAAGPSIEARTG 195 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.142 0.466 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 17,742,753 Number of extensions: 745197 Number of successful extensions: 1078 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 1069 Number of HSP's successfully gapped: 16 Length of query: 221 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 119 Effective length of database: 26,614,363 Effective search space: 3167109197 Effective search space used: 3167109197 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 114 (48.5 bits)