BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0041.p (314 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM1326 [G] COG1105 Fructose-1-phosphate kinase and related fruc... 222 5e-58 ZpfkB [G] COG1105 Fructose-1-phosphate kinase and related fructo... 218 1e-56 pfkB [G] COG1105 Fructose-1-phosphate kinase and related fructos... 218 1e-56 ZpfkB [G] COG1105 Fructose-1-phosphate kinase and related fructo... 217 2e-56 ECs2429 [G] COG1105 Fructose-1-phosphate kinase and related fruc... 217 2e-56 >STM1326 [G] COG1105 Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB) Length = 310 Score = 222 bits (566), Expect = 5e-58, Method: Compositional matrix adjust. Identities = 124/307 (40%), Positives = 185/307 (60%), Gaps = 2/307 (0%) Query: 2 IVTLTVNPSVDASTGIDRVVPDHKLRCREASYKPGGGGVNVSRAIHRLGGESLALYASGG 61 I TLT+ PS+D++T ++ P+ KLRC ++PGGGG+NV+RAI LGG + A++ +GG Sbjct: 4 IYTLTLAPSLDSATITPQIYPEGKLRCSAPVFEPGGGGINVARAIAHLGGTATAIFPAGG 63 Query: 62 LHGQLLHEMLEQEGVGHQEIPISGQTRENLIVVEESTGQQFRFDMPGPHFIEGDWQQCLE 121 G+ L +L E V + TR+NL V ES+G+Q+RF MPG + +++Q E Sbjct: 64 ATGEHLVALLADENVPVSTVDAKDWTRQNLHVHVESSGEQYRFVMPGATLDDDEFRQLEE 123 Query: 122 QLKSLGEKPTYIVASGSLPPGCPADFYGRVIEAVKPWQARVIVDTSGEALQKAADAG-VY 180 Q+ + E +V SGSLPPG + ++I A + R I+D+SG+AL A G + Sbjct: 124 QVLEI-ESGAILVISGSLPPGVKVEKLTQLISAAQKQGIRCIIDSSGDALTAALALGDIE 182 Query: 181 LLKPNARELEELAGHSITGDAEVRAAALELIEQGRTEVVVVSLGGEGALLITREGCEHLK 240 L+KPN +EL L +T +VR AA EL++ G+ VVVSLG +GAL I E C + Sbjct: 183 LVKPNLKELSALVNRDLTQPDDVRKAAQELVQSGKARRVVVSLGPQGALGIDSENCIQVV 242 Query: 241 APEVPVLSVVGAGDSLVGGLVYSLERGWPLRRAVQFGIASGAAAVMNPERQLCKREDAER 300 P V S VGAGDS+VG + L + L V+FG+A+G+AA +N +LC R+D ++ Sbjct: 243 PPPVKSQSTVGAGDSMVGAMTLKLAQDASLEEMVRFGVAAGSAATLNQGTRLCSRDDTQK 302 Query: 301 LFKQIEA 307 ++ + A Sbjct: 303 IYAYLSA 309 >ZpfkB [G] COG1105 Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB) Length = 309 Score = 218 bits (554), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 122/302 (40%), Positives = 181/302 (59%), Gaps = 2/302 (0%) Query: 2 IVTLTVNPSVDASTGIDRVVPDHKLRCREASYKPGGGGVNVSRAIHRLGGESLALYASGG 61 I TLT+ PS+D++T ++ P+ KLRC ++PGGGG+NV+RAI LGG + A++ +GG Sbjct: 4 IYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINVARAIAHLGGSATAIFPAGG 63 Query: 62 LHGQLLHEMLEQEGVGHQEIPISGQTRENLIVVEESTGQQFRFDMPGPHFIEGDWQQCLE 121 G+ L +L E V + TR+NL V E++G+Q+RF MPG E +++Q E Sbjct: 64 ATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGEQYRFVMPGAALNEDEFRQLEE 123 Query: 122 QLKSLGEKPTYIVASGSLPPGCPADFYGRVIEAVKPWQARVIVDTSGEALQKAADAG-VY 180 Q+ + E +V SGSLPPG + ++I A + R IVD+SGEAL A G + Sbjct: 124 QVLEI-ESGAILVISGSLPPGVKLEKLTQLISAAQKQGIRCIVDSSGEALSAALAIGNIE 182 Query: 181 LLKPNARELEELAGHSITGDAEVRAAALELIEQGRTEVVVVSLGGEGALLITREGCEHLK 240 L+KPN +EL L +T +VR AA E++ G+ + VVVSLG +GAL + E C + Sbjct: 183 LVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKAKRVVVSLGPQGALGVDSENCIQVV 242 Query: 241 APEVPVLSVVGAGDSLVGGLVYSLERGWPLRRAVQFGIASGAAAVMNPERQLCKREDAER 300 P V S VGAGDS+VG + L L V+FG+A+G+AA +N +LC +D ++ Sbjct: 243 PPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMVRFGVAAGSAATLNQGTRLCSHDDTQK 302 Query: 301 LF 302 ++ Sbjct: 303 IY 304 >pfkB [G] COG1105 Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB) Length = 309 Score = 218 bits (554), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 122/302 (40%), Positives = 181/302 (59%), Gaps = 2/302 (0%) Query: 2 IVTLTVNPSVDASTGIDRVVPDHKLRCREASYKPGGGGVNVSRAIHRLGGESLALYASGG 61 I TLT+ PS+D++T ++ P+ KLRC ++PGGGG+NV+RAI LGG + A++ +GG Sbjct: 4 IYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINVARAIAHLGGSATAIFPAGG 63 Query: 62 LHGQLLHEMLEQEGVGHQEIPISGQTRENLIVVEESTGQQFRFDMPGPHFIEGDWQQCLE 121 G+ L +L E V + TR+NL V E++G+Q+RF MPG E +++Q E Sbjct: 64 ATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGEQYRFVMPGAALNEDEFRQLEE 123 Query: 122 QLKSLGEKPTYIVASGSLPPGCPADFYGRVIEAVKPWQARVIVDTSGEALQKAADAG-VY 180 Q+ + E +V SGSLPPG + ++I A + R IVD+SGEAL A G + Sbjct: 124 QVLEI-ESGAILVISGSLPPGVKLEKLTQLISAAQKQGIRCIVDSSGEALSAALAIGNIE 182 Query: 181 LLKPNARELEELAGHSITGDAEVRAAALELIEQGRTEVVVVSLGGEGALLITREGCEHLK 240 L+KPN +EL L +T +VR AA E++ G+ + VVVSLG +GAL + E C + Sbjct: 183 LVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKAKRVVVSLGPQGALGVDSENCIQVV 242 Query: 241 APEVPVLSVVGAGDSLVGGLVYSLERGWPLRRAVQFGIASGAAAVMNPERQLCKREDAER 300 P V S VGAGDS+VG + L L V+FG+A+G+AA +N +LC +D ++ Sbjct: 243 PPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMVRFGVAAGSAATLNQGTRLCSHDDTQK 302 Query: 301 LF 302 ++ Sbjct: 303 IY 304 Score = 217 bits (553), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 122/302 (40%), Positives = 181/302 (59%), Gaps = 2/302 (0%) Query: 2 IVTLTVNPSVDASTGIDRVVPDHKLRCREASYKPGGGGVNVSRAIHRLGGESLALYASGG 61 I TLT+ PS+D++T ++ P+ KLRC ++PGGGG+NV+RAI LGG + A++ +GG Sbjct: 4 IYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINVARAIAHLGGSATAIFPAGG 63 Query: 62 LHGQLLHEMLEQEGVGHQEIPISGQTRENLIVVEESTGQQFRFDMPGPHFIEGDWQQCLE 121 G+ L +L E V + TR+NL V E++G+Q+RF MPG E +++Q E Sbjct: 64 ATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGEQYRFVMPGAALNEDEFRQLEE 123 Query: 122 QLKSLGEKPTYIVASGSLPPGCPADFYGRVIEAVKPWQARVIVDTSGEALQKAADAG-VY 180 Q+ + E +V SGSLPPG + ++I A + R IVD+SGEAL A G + Sbjct: 124 QVLEI-ESGAILVISGSLPPGVKLEKLTQLISAAQKQGIRCIVDSSGEALSAALAIGNIE 182 Query: 181 LLKPNARELEELAGHSITGDAEVRAAALELIEQGRTEVVVVSLGGEGALLITREGCEHLK 240 L+KPN +EL L +T +VR AA E++ G+ + VVVSLG +GAL + E C + Sbjct: 183 LVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKAKRVVVSLGPQGALGVDSENCIQVV 242 Query: 241 APEVPVLSVVGAGDSLVGGLVYSLERGWPLRRAVQFGIASGAAAVMNPERQLCKREDAER 300 P V S VGAGDS+VG + L L V+FG+A+G+AA +N +LC +D ++ Sbjct: 243 PPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMVRFGVAAGSAATLNQGTRLCSHDDTQK 302 Query: 301 LF 302 ++ Sbjct: 303 IY 304 >ECs2429 [G] COG1105 Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB) Length = 309 Score = 217 bits (553), Expect = 2e-56, Method: Compositional matrix adjust. Identities = 121/302 (40%), Positives = 181/302 (59%), Gaps = 2/302 (0%) Query: 2 IVTLTVNPSVDASTGIDRVVPDHKLRCREASYKPGGGGVNVSRAIHRLGGESLALYASGG 61 I TLT+ PS+D++T ++ P+ KLRC ++PGGGG+NV+RAI LGG + A++ +GG Sbjct: 4 IYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINVARAIAHLGGSATAIFPAGG 63 Query: 62 LHGQLLHEMLEQEGVGHQEIPISGQTRENLIVVEESTGQQFRFDMPGPHFIEGDWQQCLE 121 G+ L +L E V + TR+NL V E++G+Q+RF MPG E +++Q E Sbjct: 64 ATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGEQYRFVMPGAALNEDEFRQLEE 123 Query: 122 QLKSLGEKPTYIVASGSLPPGCPADFYGRVIEAVKPWQARVIVDTSGEALQKAADAG-VY 180 Q+ + E +V SGSLPPG + ++I A + R I+D+SGEAL A G + Sbjct: 124 QVLEI-ESGAILVISGSLPPGVKLEKLTQLISAAQNQGIRCIIDSSGEALSAALAIGNIE 182 Query: 181 LLKPNARELEELAGHSITGDAEVRAAALELIEQGRTEVVVVSLGGEGALLITREGCEHLK 240 L+KPN +EL L +T +VR AA E++ G+ + VVVSLG +GAL + E C + Sbjct: 183 LVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKAKRVVVSLGPQGALGVDSENCIQVV 242 Query: 241 APEVPVLSVVGAGDSLVGGLVYSLERGWPLRRAVQFGIASGAAAVMNPERQLCKREDAER 300 P V S VGAGDS+VG + L L V+FG+A+G+AA +N +LC +D ++ Sbjct: 243 PPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMVRFGVAAGSAATLNQGTRLCSHDDTQK 302 Query: 301 LF 302 ++ Sbjct: 303 IY 304 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.136 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,249,122 Number of extensions: 1075538 Number of successful extensions: 2613 Number of sequences better than 1.0e-05: 44 Number of HSP's gapped: 2582 Number of HSP's successfully gapped: 44 Length of query: 314 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 208 Effective length of database: 26,153,027 Effective search space: 5439829616 Effective search space used: 5439829616 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)