BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0040.p (314 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC1852 [P] COG0598 Mg2+ and Co2+ transporters 183 3e-46 BS_yfjQ [P] COG0598 Mg2+ and Co2+ transporters 181 1e-45 NMA1721 [I] COG1562 Phytoene/squalene synthetase 175 1e-43 STM0561 [T] COG0642 Signal transduction histidine kinase 172 7e-43 sll0671 [P] COG0598 Mg2+ and Co2+ transporters 166 4e-41 >CAC1852 [P] COG0598 Mg2+ and Co2+ transporters Length = 354 Score = 183 bits (465), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 103/312 (33%), Positives = 184/312 (58%), Gaps = 18/312 (5%) Query: 17 VDDIEETLT-APTEGFYWIDAD-VEDLELLQPL---YNLHDLAVEDCLSEEDQRPKLEIY 71 V I+E L T+ WI+ + +ED++L + + + +H L +ED L+ +QRPK+E + Sbjct: 47 VHSIDECLKYRDTDNMTWININGLEDIDLYEKVGKYFGVHSLIMEDILNT-NQRPKIEEF 105 Query: 72 ESHYFIVVNSIRFDDEEIFLRALNVFLGRHFIITVTKQKIH---------ELRVLKPILW 122 E + FIV+ I F ++++ + +++ + IIT ++ +R K + Sbjct: 106 EKYIFIVLKMIYFREDKLVVEQVSIICMENLIITFQEEGKDGDVFGNLRDRIRNTKSKIR 165 Query: 123 EQEVSEPDRFLYLLIDLVVDNYFSVGDRIEARIEKLEEDILMHTKKSHLSEIIGLRSEIL 182 + V Y LID +VDNYF + +++E +IE EE ++ + +E+ L+ +++ Sbjct: 166 KTGVG---YLTYALIDSIVDNYFVILEKVEDKIENFEESLMTTATNNLFNEVYNLKRQMI 222 Query: 183 WLKKMLGPQKEVINTLNKKDLRLIDDQLQKYFSDIYENAVKISETFETYRDLMGNLREAY 242 +L K + P +E+INTL + ++ +I + YF D+Y++ V++ +T E +RD++ L + Y Sbjct: 223 FLWKAVWPLREIINTLQRGEVEIIGSDISIYFKDVYDHTVQVIDTIELFRDMIAGLLDTY 282 Query: 243 QSSIANRANEIMRVFTAITTIFMPLTVITGIYGMNFDNIPETHSQYGYYGVIAVMVTLGC 302 SS +N+ NEIM+ T +TIF+PL+ + G+YGMNFD +PE +YGY + +M++L Sbjct: 283 LSSASNKMNEIMKFLTIFSTIFIPLSFLVGVYGMNFDYMPELKFRYGYAVLWVIMISLSA 342 Query: 303 GMLVVFRKKDWL 314 ML+ FRKK WL Sbjct: 343 FMLMYFRKKKWL 354 >BS_yfjQ [P] COG0598 Mg2+ and Co2+ transporters Length = 273 Score = 181 bits (459), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 0/305 (0%), Positives = 0/305 (0%), Gaps = 13/305 (4%) Query: 16 PVDDIEETLTAPTEGFYWID-ADVEDLE--LLQPLYNLHDLAVEDCLSEEDQRPKLEIYE 72 EE + D + + + L + Sbjct: 18 RPLTREE----LFRVVPSVFSEDKHESRSERYTYIPTISLLDSLQRE-GFQPFFACQTRV 72 Query: 73 SHYFIVVNSIRFDDEEIFLRALNVFLGRHFIITVTKQKIHELRVLKPILWEQE---VSEP 129 ++ + II + + P ++ Sbjct: 73 RDPRRREHTK--HMLRLRREGQITGKQVPEIILLNSHDGTSSYQMLPGMFRAVCQNGLVC 130 Query: 130 DRFLYLLIDLVVDNYFSVGDRIEARIEKLEEDILMHTKKSHLSEIIGLRSEILWLKKMLG 189 + + S + + E + + + L + Sbjct: 131 GESFGEVRVPHKGDVVSQVIEGAYEVLGIFERVEEKRDAMQSLLLPPPVQQALAKAALTY 190 Query: 190 PQKEVINTLNKKDLRLIDDQLQKYFSDIYENAVKISETFETYRDLMGNLREAYQSSIANR 249 E + + + + + + Sbjct: 191 RFGEDHQPVTESQILSPRRWQDESNDLWTTYQRIQENLIKGGLSGRNAKGGRTHTRAVRG 250 Query: 250 ANEIMRVFTAITTIFMPLTVITGIYGMNFDNIPETHSQYGYYGVIAVMVTLGCGMLVVFR 309 Sbjct: 251 310 Query: 310 KKDWL 314 Sbjct: 311 315 >NMA1721 [I] COG1562 Phytoene/squalene synthetase Length = 346 Score = 175 bits (443), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 0/297 (0%), Positives = 0/297 (0%), Gaps = 21/297 (7%) Query: 33 WIDAD----VEDLELLQPLYNLHDLAVEDCLSEEDQRPKLEIYESHYFIVVNSIRFD--D 86 + + + + + ++ + +S++ D Sbjct: 66 ETYVGNWLAKHGSREKLIIASKVSGPSRNNDKG-IRPDQALDRKNIREALHDSLKRLQTD 124 Query: 87 EEIFLRALNVFLGRHFIITVTKQKIHELRVLKPILWEQEVSEP---------DRFLYLLI 137 + + + + + + Sbjct: 125 YLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVS-----LLDTLDALAEYQRAGKIRYIGVS 179 Query: 138 DLVVDNYFSVGDRIEARIEKLEEDILMHTKKSHLSEIIGLRSEILWLKKMLGPQKEVINT 197 + + I + S +GL + L + Sbjct: 180 NETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFG 239 Query: 198 LNKKDLRLIDDQLQKYFSDIYENAVKISETFETYRDLMGNLREAYQSSIANRANEIMRVF 257 + E + ++ + A A +R Sbjct: 240 TLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQ 299 Query: 258 TAITTIFMPLTVITGIYGMNFDNIPETHSQYGYYGVIAVMVTLGCGMLVVFRKKDWL 314 Sbjct: 300 356 >STM0561 [T] COG0642 Signal transduction histidine kinase Length = 109 Score = 172 bits (436), Expect = 7e-43, Method: Compositional matrix adjust. Identities = 0/275 (0%), Positives = 0/275 (0%), Gaps = 9/275 (3%) Query: 47 LYNLHDLAVEDCLSEEDQRPKLEIYESHYFIVVNSIRFDD--EEIFLRALNVFLGRHFII 104 + + ++ + Sbjct: 79 KSIVTAHRGKISVTSD- 137 Query: 105 TVTKQKIHEL--RVLKPILWEQEV---SEPDRFLYLLIDLVVDNYFSVGDRIEARIEKLE 159 Sbjct: 138 - 196 Query: 160 EDILMHTKKSHLSEIIGLRSEILWLKKMLGPQKEVINTLNKKDLRLIDDQLQKYFSDIYE 219 Sbjct: 197 256 Query: 220 NAVKISETFETYRDLMGNLREAYQSSIANRANEIMRVFTAITTIFMPLTVITGIYGMNFD 279 Sbjct: 257 316 Query: 280 NIPETHSQYGYYGVIAVMVTLGCGMLVVFRKKDWL 314 Sbjct: 317 351 >sll0671 [P] COG0598 Mg2+ and Co2+ transporters Length = 380 Score = 166 bits (420), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 98/307 (31%), Positives = 172/307 (56%), Gaps = 24/307 (7%) Query: 28 TEGFYWIDADV----EDLELLQPLYNLHDLAVEDCLSEEDQRPKLEIYESHYFIVVNSIR 83 TE WI+ D E L ++ LH +A+ED ++ QRPK+ YE+H + + Sbjct: 67 TESVSWINIDGLGNHTTWEQLGEVFKLHPVALEDIVNVP-QRPKVVEYENHLIFISRMVT 125 Query: 84 FD-DEEIFLR-ALNVFLGRHFIITVTKQKIHE--------LRVLKPILWEQEVSEPDRFL 133 D + F+ ++ LG+H+++T+ ++ ++ +R K + ++ D Sbjct: 126 LDQSSQTFISEQISFILGKHYLLTIQEEPKYDCLFSVRERIRTKKGAIRQKNA---DYLF 182 Query: 134 YLLIDLVVDNYFSVGDRIEARIEKLEEDILMHTKKSHLSEIIGLRSEILWLKKMLGPQKE 193 Y LID ++D +F V + ++ L+ +I+ L++I L+ ++L +++ + PQ++ Sbjct: 183 YALIDAIIDGFFPVMEVYGELVQSLQSEIISCPTNKSLAKIHQLQQDLLIMRRAIWPQRD 242 Query: 194 VINTLNKKDLRLIDDQLQKYFSDIYENAVKISETFETYRDLMGNLREAYQSSIANRANEI 253 IN+L + LI D+++ + D Y++ ++I + ETYRDL NL + Y SS++NR NEI Sbjct: 243 AINSLLRDGSDLISDEVRVFLRDCYDHTIQILDMIETYRDLASNLTDIYLSSVSNRMNEI 302 Query: 254 MRVFTAITTIFMPLTVITGIYGMNFD------NIPETHSQYGYYGVIAVMVTLGCGMLVV 307 M+ T I++IF+PLT I GIYGMNF+ N+PE + +GY + VM+T+G ML Sbjct: 303 MKTLTVISSIFIPLTFIAGIYGMNFNPDKSPWNMPELNWYWGYPVIWMVMLTVGGMMLYF 362 Query: 308 FRKKDWL 314 F +K W Sbjct: 363 FWRKGWF 369 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.141 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,204,672 Number of extensions: 948753 Number of successful extensions: 2000 Number of sequences better than 1.0e-05: 48 Number of HSP's gapped: 1972 Number of HSP's successfully gapped: 49 Length of query: 314 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 208 Effective length of database: 26,153,027 Effective search space: 5439829616 Effective search space used: 5439829616 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)