BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0038.p (399 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1627 [E] COG0626 Cystathionine beta-lyases/cystathionine gamma... 424 e-119 BS_yjcI [E] COG0626 Cystathionine beta-lyases/cystathionine gamm... 423 e-118 CAC0390 [E] COG0626 Cystathionine beta-lyases/cystathionine gamm... 409 e-114 BS_yrhB [E] COG0626 Cystathionine beta-lyases/cystathionine gamm... 393 e-109 PM0995 [E] COG0626 Cystathionine beta-lyases/cystathionine gamma... 385 e-107 >BH1627 [E] COG0626 Cystathionine beta-lyases/cystathionine gamma-synthases Length = 378 Score = 424 bits (1091), Expect = e-119, Method: Compositional matrix adjust. Identities = 208/378 (55%), Positives = 267/378 (70%), Gaps = 17/378 (4%) Query: 1 MDEKLRIESRLAQIGSQEDPATGAINYPIYNATAFRHPRLGQSTGFDYIRTKNPTRSVLE 60 M+ K +E+ L QIG++ D TGAIN P+Y +TA+RH +G+STG+DY RT NPTR VLE Sbjct: 1 MNRK-ELETALVQIGNRMDDRTGAINTPVYFSTAYRHSGIGESTGYDYARTGNPTREVLE 59 Query: 61 EAVAELESGDAAFACSSGMAALTTVFALFGQGDHLVVSLDLYGGTYRLLERILSKYGISA 120 +A+A LE+GD FACSSGMAA+ TVF+LF GD ++ S DLYGGTYRL E K+G+S Sbjct: 60 KAIATLENGDQGFACSSGMAAIQTVFSLFQSGDEIIASQDLYGGTYRLFEGGWKKWGLSF 119 Query: 121 SYVDTNDLDGLEASRRPNTKAVFIETPTNPLMMITDIEAVCTWSRRHGLLTIVDNTLLTP 180 SY D +L LE T+A+FIETPTNPLM +I + + +HGLL IVDNT TP Sbjct: 120 SYADPRNLAALEQQITEKTRALFIETPTNPLMQEANIRELAALANKHGLLLIVDNTFYTP 179 Query: 181 FFQRPLELGADIIVHSATKYLGGHNDVLAGLIVTKGTELSAEMAILHNSLGAVLAPNDSY 240 Q+PL G I++HSA+KYLGGHNDV+AGLIV KG EL ++A HN +G L+ DS+ Sbjct: 180 LLQQPLNEGTHIVIHSASKYLGGHNDVIAGLIVAKGQELCEQIAYYHNGIGGTLSAFDSW 239 Query: 241 QLMKGMKTLALRMERHESNALALARYLLEHPAIAEVFHPGLPDHPGYDIQKRQSSGNTGI 300 L++GMKTLALRME+H++NA A+A YL +H + +V +P G G+ Sbjct: 240 LLIRGMKTLALRMEQHQNNARAIASYLEKHEGVTDVLYP----------------GRGGM 283 Query: 301 FSFKVKDAGYVEPLLRHIRLIAFAESLGGVESLMTYPAVQTHADIPVEIRDAVGVDDRLL 360 SF+++ +V P L+ ++LI+FAESLGGVESLMTYPA QTHADIP ++R A GV +RLL Sbjct: 284 LSFRIQSESWVNPFLQSLKLISFAESLGGVESLMTYPATQTHADIPEDVRIANGVCNRLL 343 Query: 361 RFSVGIEHADDLIADLGQ 378 RFSVGIEH DLIADL Q Sbjct: 344 RFSVGIEHVGDLIADLDQ 361 >BS_yjcI [E] COG0626 Cystathionine beta-lyases/cystathionine gamma-synthases Length = 211 Score = 423 bits (1087), Expect = e-118, Method: Compositional matrix adjust. Identities = 0/372 (0%), Positives = 0/372 (0%), Gaps = 16/372 (4%) Query: 7 IESRLAQIGSQEDPATGAINYPIYNATAFRHPRLGQSTGFDYIRTKNPTRSVLEEAVAEL 66 + + +P L + + A + Sbjct: 5 KRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAAM 64 Query: 67 ESGDAAFACSSGMAALTTVFALFGQGDHLVVSLDLYGGTYRLLERILSKYGISASYVDTN 126 + + L+ + G L Sbjct: 65 LELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRKTANV 124 Query: 127 DLDGLEASRRPNTKAVFIETPTNPLMMITDIEAVCTWSRRHGLLTIVDNTLLTPFFQRPL 186 L+ + Sbjct: 125 VLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLILHGR 184 Query: 187 ELGADIIVHSATKYLGGHNDVLAGLIVTKGTELSAEMAILHNSLGAVLAPNDSYQLMKGM 246 Sbjct: 185 244 Query: 247 KTLALRMERHESNALALARYLLEHPAIAEVFHPGLPDHPGYDIQKRQSSGNTGIFSFKVK 306 Sbjct: 245 ---------------- 288 Query: 307 DAGYVEPLLRHIRLIAFAESLGGVESLMTYPAVQTHADIPVEIRDAVGVDDRLLRFSVGI 366 Sbjct: 289 348 Query: 367 EHADDLIADLGQ 378 Sbjct: 349 360 >CAC0390 [E] COG0626 Cystathionine beta-lyases/cystathionine gamma-synthases Length = 384 Score = 409 bits (1051), Expect = e-114, Method: Compositional matrix adjust. Identities = 191/357 (53%), Positives = 256/357 (71%) Query: 22 TGAINYPIYNATAFRHPRLGQSTGFDYIRTKNPTRSVLEEAVAELESGDAAFACSSGMAA 81 TGAI+YPIY ++ FRH L + TG+DY RT NPTR +E+ VA LE+G A A SSGMAA Sbjct: 24 TGAISYPIYQSSTFRHEGLNKGTGYDYSRTGNPTRDEVEKTVAALENGRACLAYSSGMAA 83 Query: 82 LTTVFALFGQGDHLVVSLDLYGGTYRLLERILSKYGISASYVDTNDLDGLEASRRPNTKA 141 +++V +F GDH++VS DLYGGTYR+ E I YGI +Y DT + +E R NTKA Sbjct: 84 ISSVLTIFKGGDHIIVSDDLYGGTYRIFEEIYEHYGIEVTYTDTTSTENIEKELRENTKA 143 Query: 142 VFIETPTNPLMMITDIEAVCTWSRRHGLLTIVDNTLLTPFFQRPLELGADIIVHSATKYL 201 +++ETPTNPLM ITDI V ++ H L IVDNT +TP++Q+PLELGADI++HS TKYL Sbjct: 144 IYLETPTNPLMKITDIREVSKLAKEHNTLLIVDNTFMTPYYQKPLELGADIVLHSGTKYL 203 Query: 202 GGHNDVLAGLIVTKGTELSAEMAILHNSLGAVLAPNDSYQLMKGMKTLALRMERHESNAL 261 GHND LAG ++ L ++ + NS+GAVLAP DS+ +++G+KTL +R++R + NA+ Sbjct: 204 CGHNDALAGFVILNDERLIEKLRFIQNSVGAVLAPFDSWLILRGIKTLHIRLDRQQENAI 263 Query: 262 ALARYLLEHPAIAEVFHPGLPDHPGYDIQKRQSSGNTGIFSFKVKDAGYVEPLLRHIRLI 321 +A +L +H I +V +PGL +H G+DI K ++SG + SF V VE +L +++I Sbjct: 264 KIANFLKKHKKITKVLYPGLEEHVGHDILKSEASGFGAMISFYVDSKETVEKVLESVKVI 323 Query: 322 AFAESLGGVESLMTYPAVQTHADIPVEIRDAVGVDDRLLRFSVGIEHADDLIADLGQ 378 FAESLGGVESL+TYP QTHADIP +IR +GV D+LLRFSVGIE+ DDLI DL + Sbjct: 324 IFAESLGGVESLITYPYTQTHADIPDDIRKRLGVTDKLLRFSVGIENVDDLIKDLDK 380 >BS_yrhB [E] COG0626 Cystathionine beta-lyases/cystathionine gamma-synthases Length = 379 Score = 393 bits (1010), Expect = e-109, Method: Compositional matrix adjust. Identities = 183/360 (50%), Positives = 259/360 (71%) Query: 19 DPATGAINYPIYNATAFRHPRLGQSTGFDYIRTKNPTRSVLEEAVAELESGDAAFACSSG 78 D TGA++ PIY + ++ P+ GQ TG++Y RT NPTR+ LE V ELESG+A +A SSG Sbjct: 15 DEKTGAVSVPIYQVSTYKQPKAGQHTGYEYSRTANPTRTALEALVTELESGEAGYAFSSG 74 Query: 79 MAALTTVFALFGQGDHLVVSLDLYGGTYRLLERILSKYGISASYVDTNDLDGLEASRRPN 138 MAA+T V LF GDH+V++ D+YGGTYR++ ++L++ GI +++VDT+ + +E + RPN Sbjct: 75 MAAITAVMMLFNSGDHVVLTDDVYGGTYRVMTKVLNRLGIESTFVDTSSREEVEKAIRPN 134 Query: 139 TKAVFIETPTNPLMMITDIEAVCTWSRRHGLLTIVDNTLLTPFFQRPLELGADIIVHSAT 198 TKA++IETPTNPL+ ITD+ + +++ G+L IVDNT TP+FQ+PL LGADI++HSAT Sbjct: 135 TKAIYIETPTNPLLKITDLTLMADIAKKAGVLLIVDNTFNTPYFQQPLTLGADIVLHSAT 194 Query: 199 KYLGGHNDVLAGLIVTKGTELSAEMAILHNSLGAVLAPNDSYQLMKGMKTLALRMERHES 258 KYLGGH+DV+ GL+VT EL E+ + NS G VL P DS+ LM+G+KTL LRME + Sbjct: 195 KYLGGHSDVVGGLVVTASKELGEELHFVQNSTGGVLGPQDSWLLMRGIKTLGLRMEAIDQ 254 Query: 259 NALALARYLLEHPAIAEVFHPGLPDHPGYDIQKRQSSGNTGIFSFKVKDAGYVEPLLRHI 318 NA +A +L HPA+ +++PG +HPG+++ K Q +G G+ SF + V+ L ++ Sbjct: 255 NARKIASFLENHPAVQTLYYPGSSNHPGHELAKTQGAGFGGMISFDIGSEERVDAFLGNL 314 Query: 319 RLIAFAESLGGVESLMTYPAVQTHADIPVEIRDAVGVDDRLLRFSVGIEHADDLIADLGQ 378 +L AESLG VESL++ PA THA IP E R +G+ D L+R SVGIE A+DL+ D+GQ Sbjct: 315 KLFTIAESLGAVESLISVPARMTHASIPRERRLELGITDGLIRISVGIEDAEDLLEDIGQ 374 >PM0995 [E] COG0626 Cystathionine beta-lyases/cystathionine gamma-synthases Length = 369 Score = 385 bits (989), Expect = e-107, Method: Compositional matrix adjust. Identities = 191/379 (50%), Positives = 255/379 (67%), Gaps = 17/379 (4%) Query: 1 MDEKLRIESRLAQIGSQEDPATGAINYPIYNATAFRHPRLGQSTGFDYIRTKNPTRSVLE 60 M + IE+ LAQ G++ D TGA++ PI+ +TA+ H +G+STG+DY RTKNPTRSVLE Sbjct: 1 MTQHYSIETLLAQAGNRTDERTGAVSTPIFLSTAYAHHGIGESTGYDYTRTKNPTRSVLE 60 Query: 61 EAVAELESGDAAFACSSGMAALTTVFALFGQGDHLVVSLDLYGGTYRLLERILSK-YGIS 119 E +A+LE G+ FAC+SGMAA+ + +LF D +VS D+YGGTYRLL+ G+ Sbjct: 61 ETIAKLEGGERGFACASGMAAIQLIMSLFTSPDEWIVSRDVYGGTYRLLDFAYKNTQGVK 120 Query: 120 ASYVDTNDLDGLEASRRPNTKAVFIETPTNPLMMITDIEAVCTWSRRHGLLTIVDNTLLT 179 YV+T+++ +EA+ NTKA+F+ETP+NPLM D+ A+ +++H LL IVDNT LT Sbjct: 121 PVYVNTSEVSCIEAAITSNTKAIFVETPSNPLMEECDVAAIAKIAKKHNLLLIVDNTFLT 180 Query: 180 PFFQRPLELGADIIVHSATKYLGGHNDVLAGLIVTKGTELSAEMAILHNSLGAVLAPNDS 239 P RP+E GADI++HS TKYL GHND L GL+V KG EL + + N GAVL+P DS Sbjct: 181 PVLFRPIEAGADIVIHSGTKYLAGHNDALVGLVVAKGEELCQRLFYIQNGAGAVLSPFDS 240 Query: 240 YQLMKGMKTLALRMERHESNALALARYLLEHPAIAEVFHPGLPDHPGYDIQKRQSSGNTG 299 + ++GMKTLALRMERHE NA LA +L P + V +P G Sbjct: 241 WLTIRGMKTLALRMERHEQNAKQLAAFLASQPQVKNVLYP----------------NKGG 284 Query: 300 IFSFKVKDAGYVEPLLRHIRLIAFAESLGGVESLMTYPAVQTHADIPVEIRDAVGVDDRL 359 + SF++ +A +V P L+ ++LI FAESLGG ES +TYPA QTH DIP E R A GV + L Sbjct: 285 MLSFRLHEAHWVNPFLKALKLITFAESLGGTESFITYPATQTHMDIPEEERIARGVCNCL 344 Query: 360 LRFSVGIEHADDLIADLGQ 378 LRFSVG+E+ +D+ ADL Q Sbjct: 345 LRFSVGLENVEDIKADLLQ 363 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,495,257 Number of extensions: 1258599 Number of successful extensions: 2694 Number of sequences better than 1.0e-05: 105 Number of HSP's gapped: 2427 Number of HSP's successfully gapped: 107 Length of query: 399 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 290 Effective length of database: 25,807,025 Effective search space: 7484037250 Effective search space used: 7484037250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)