BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0036.p (255 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3143 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and relate... 338 3e-93 aq_789 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and relate... 209 3e-54 Cj0462 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and relate... 209 4e-54 AF0390 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and relate... 202 6e-52 HP0656 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and relate... 198 6e-51 >BH3143 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes Length = 370 Score = 338 bits (868), Expect = 3e-93, Method: Compositional matrix adjust. Identities = 164/245 (66%), Positives = 195/245 (79%), Gaps = 9/245 (3%) Query: 12 ITMHSFSPAEIMKMVEVSGLPLEQVVREIHAAGLDSLPGGGAEILDDRTRRKISRLKGSW 71 I MHSFSPAE+ K+ EVS + +E+V+RE+H AGLDS+PGGGAEIL + TR ++SRLK +W Sbjct: 134 IWMHSFSPAEVWKIAEVSSMSVEEVLRELHEAGLDSMPGGGAEILTEETRLRVSRLKITW 193 Query: 72 REWMDVMQTAHKIGMNTTATMVIGLGESMEERALHLLRVREAQDECIANKYDSEGFLAFI 131 +W++ M+ K+GM+ TATMVIG GES EERALHL RVR+AQDE +E F AFI Sbjct: 194 EQWINAMKATKKVGMHGTATMVIGFGESFEERALHLQRVRDAQDE-------TECFTAFI 246 Query: 132 SWTFQPDNTNL-KLDRQTPEEYLKTVAISRLVLDNIKNFQSSWVTMGPEVGKLSLQYGCN 190 SW FQP+NT + K + TP +YLK VAISRL LDNI NFQSSWVTMGPEVGKLSLQYGCN Sbjct: 247 SWLFQPENTGMYKTKKLTPRDYLKNVAISRLFLDNIPNFQSSWVTMGPEVGKLSLQYGCN 306 Query: 191 DFGSTMIEENVVSSAGATHKVNIESITQIIREAGKIPAQRNTRYDILRVFDDADAKIDND 250 DFGSTMIEENVVS+AG THKVN I Q+IREAGKIPAQR T Y+I+R F+D +A + D Sbjct: 307 DFGSTMIEENVVSAAGTTHKVNTNKILQLIREAGKIPAQRTTSYEIIRTFEDKEAA-EKD 365 Query: 251 FVMQN 255 FVMQN Sbjct: 366 FVMQN 370 >aq_789 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes Length = 361 Score = 209 bits (532), Expect = 3e-54, Method: Compositional matrix adjust. Identities = 110/232 (47%), Positives = 150/232 (64%), Gaps = 9/232 (3%) Query: 11 EITMHSFSPAEIMKMVEVSGLPLEQVVREIHAAGLDSLPGGGAEILDDRTRRKISRLKGS 70 ++ +H FS EI+ + + LP+E+V+R + AGL S+PGGGAEIL R KIS K + Sbjct: 136 QVQIHCFSAPEIVYLSKKEKLPIEEVIRRLKKAGLMSIPGGGAEILSQEVRDKISPGKCT 195 Query: 71 WREWMDVMQTAHKIGMNTTATMVIGLGESMEERALHLLRVREAQDECIANKYDSEGFLAF 130 EW +V +TAH++GM TTATM+ G ES+E HL RVR+ QDE + GF AF Sbjct: 196 VEEWEEVHRTAHRLGMTTTATMMFGHVESIEHIIEHLERVRKIQDE-------TGGFTAF 248 Query: 131 ISWTFQPDNTNLKL-DRQTPEEYLKTVAISRLVLDNIKNFQSSWVTMGPEVGKLSLQYGC 189 I WTF+ NT L + TP YLK +A+SR+ LDN KN QSS VT ++G + L +G Sbjct: 249 IPWTFKKGNTELDFVEEATPTYYLKVLALSRIYLDNFKNIQSSHVTQTMQIGLIGLHFGA 308 Query: 190 NDFGSTMIEENVVSSAGATHKV-NIESITQIIREAGKIPAQRNTRYDILRVF 240 ND GS MIEENV+SS ++ +E + + I+ AG IPAQR+T Y+I++VF Sbjct: 309 NDLGSVMIEENVISSTNFKVRIPKVEEMVRAIKSAGFIPAQRDTYYNIIKVF 360 >Cj0462 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes Length = 348 Score = 209 bits (532), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 107/234 (45%), Positives = 155/234 (66%), Gaps = 12/234 (5%) Query: 12 ITMHSFSPAEIMKMVEVSGLPLEQVVREIHAAGLDSLPGGGAEILDDRTRRKISRLKGSW 71 IT+H FS EI + + S + + +V++ + A GL S+PG GAE+L DR R I+ K Sbjct: 122 ITVHGFSAVEIAYIAKASKISITEVLKRLQAKGLFSIPGAGAEVLSDRVRDIIAPNKCDT 181 Query: 72 REWMDVMQTAHKIGMNTTATMVIGLGESMEERALHLLRVREAQDECIANKYDSEGFLAFI 131 W++V + AHKIGM +TATM+ G E+ EE H +R+ QDE + GF AFI Sbjct: 182 ATWLEVHRQAHKIGMKSTATMMFGTVENDEEIIDHFEHLRKLQDE-------TGGFRAFI 234 Query: 132 SWTFQPDNTNL-----KLDRQTPEEYLKTVAISRLVLDNIKNFQSSWVTMGPEVGKLSLQ 186 W+FQ DNT L ++ +Q+ +YL+ +A+ RL LDN KN QSSWVT G +G+L+L+ Sbjct: 235 LWSFQSDNTALIQKHPEIMKQSSNKYLRLLALVRLYLDNFKNLQSSWVTQGSLIGQLALK 294 Query: 187 YGCNDFGSTMIEENVVSSAGATHKVNIESITQIIREAGKIPAQRNTRYDILRVF 240 +G ND GSTM+EENVVS+AGA++++N + + ++IR G+ PA+RNT Y+IL F Sbjct: 295 FGANDLGSTMMEENVVSAAGASYRMNQDEMIRLIRSLGENPAKRNTAYEILERF 348 >AF0390 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes Length = 355 Score = 202 bits (513), Expect = 6e-52, Method: Compositional matrix adjust. Identities = 108/233 (46%), Positives = 150/233 (64%), Gaps = 11/233 (4%) Query: 12 ITMHSFSPAEIMKMVEVSGLPLEQVVREIHAAGLDSLPGGGAEILDDRTRRKISRLKGSW 71 + +HS SP EI+ + ++ + +V+ + AAGLDSLPGGGAEIL D RRKIS K S Sbjct: 130 VHIHSLSPPEIVFLAKLERSSIREVLDRLKAAGLDSLPGGGAEILVDSVRRKISPNKCSS 189 Query: 72 REWMDVMQTAHKIGMNTTATMVIGLGESMEERALHLLRVREAQDECIANKYDSEGFLAFI 131 EW+ VM+TAH + M TTATM+ G ES + HL+++RE Q++ + GF AFI Sbjct: 190 DEWLRVMETAHSLNMPTTATMMFGHVESDGDIVEHLIKIRELQEK-------TGGFTAFI 242 Query: 132 SWTFQPDNTNLKLDRQ---TPEEYLKTVAISRLVLDNIKNFQSSWVTMGPEVGKLSLQYG 188 WTFQP NT L + + +P YL+ +AISR+VL+N +N Q+SW+T G E+ LSL +G Sbjct: 243 PWTFQPGNTELYAEIKRPVSPLRYLQVLAISRIVLNNFRNIQASWLTQGFEIATLSLFFG 302 Query: 189 CNDFGSTMIEENVVSSAGATHK-VNIESITQIIREAGKIPAQRNTRYDILRVF 240 NDFG TM+EENVV + G + IE I + ++ G+ A R+T Y+IL F Sbjct: 303 ANDFGGTMLEENVVRATGKDFRPAKIEEIVKAVKAVGRPVALRDTYYNILEWF 355 >HP0656 [HR] COG1060 Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes Length = 383 Score = 198 bits (504), Expect = 6e-51, Method: Compositional matrix adjust. Identities = 105/241 (43%), Positives = 154/241 (63%), Gaps = 13/241 (5%) Query: 12 ITMHSFSPAEIMKMVEVSGLPLEQVVREIHAAGLDSLPGGGAEILDDRTRRKISRLKGSW 71 IT+H FS EI + ++S L L++V+ + AGL S+PG GAE+L DR R I+ K S Sbjct: 151 ITIHGFSAVEIDYISKISKLSLKEVLERLKNAGLSSIPGAGAEVLSDRVRDVIAPKKLSS 210 Query: 72 REWMDVMQTAHKIGMNTTATMVIGLGESMEERALHLLRVREAQDECIANKYDSEGFLAFI 131 W++V + AH G+ +TATM+ G ++ E+ HL RVR+ QDE + GF AFI Sbjct: 211 DRWIEVHRMAHLCGIKSTATMMFGSVDNEEDVVEHLQRVRDLQDE-------TGGFRAFI 263 Query: 132 SWTFQPDNTNLK-----LDRQTPEEYLKTVAISRLVLDNIKNFQSSWVTMGPEVGKLSLQ 186 W+FQP+NT LK + + + YL+ +A SR+ LDNI+N QSSWVT G +G+L+L Sbjct: 264 LWSFQPNNTPLKEEIPSIKKASSNRYLRYLACSRIFLDNIQNIQSSWVTQGSMIGQLALL 323 Query: 187 YGCNDFGSTMIEENVVSSAGATHKVNIESITQIIREAGKIPAQRNTRYDILRVFDDADAK 246 +G ND GS M+EENVV +AG + +N + ++I + G + A+RNT Y+IL+ + A AK Sbjct: 324 FGANDLGSVMMEENVVKAAGTSFCMNEAGMIELIEDIGSVAAKRNTAYEILKRY-PAKAK 382 Query: 247 I 247 + Sbjct: 383 V 383 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.132 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,452,806 Number of extensions: 769540 Number of successful extensions: 1503 Number of sequences better than 1.0e-05: 36 Number of HSP's gapped: 1437 Number of HSP's successfully gapped: 36 Length of query: 255 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 151 Effective length of database: 26,383,695 Effective search space: 3983937945 Effective search space used: 3983937945 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)