BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0027.p (351 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1199 [T] COG4585 Signal transduction histidine kinase 185 1e-46 DR1556 [T] COG4585 Signal transduction histidine kinase 119 1e-26 DR0577 [T] COG4585 Signal transduction histidine kinase 113 5e-25 SP0386 [T] COG4585 Signal transduction histidine kinase 109 6e-24 BS_yhcY_2 [T] COG4585 Signal transduction histidine kinase 104 2e-22 >BH1199 [T] COG4585 Signal transduction histidine kinase Length = 351 Score = 185 bits (469), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 108/294 (36%), Positives = 168/294 (57%), Gaps = 4/294 (1%) Query: 45 LYLCAGIVLFTVIVGYMAGQ----RIQRRIDLLDLNMLQVAKGNLSVRMPESDDQSFTRV 100 L + I+L + +G +AG I+R++D + +L +G S ++ + + Sbjct: 52 LPILVPILLSMIAIGLIAGMMQSLPIKRKLDQILHGVLLYERGTFSHQIESEGEDELAEL 111 Query: 101 YHEFNMMMDTVENKMQLLQRLGEQEVIEKEKAAESAVLEERRRMARDLHDTVSQQLFAIH 160 N M + VE ++ LQRL + +E ++AV EER+R+ARDLHD VSQQLFAI Sbjct: 112 TDRLNRMAERVEEQVASLQRLSSERAKMQETVKKAAVTEERQRLARDLHDAVSQQLFAIS 171 Query: 161 MSASSLPKVLERNAEHGKTVMDQLITMSQMAQKQMRALIAQLRPVELEGRNLFEALEKWF 220 M +++ + L E + MD + M+ AQ +MRAL+ LRP ELEG++L + +E Sbjct: 172 MMTAAIKQNLHEATEEVQQQMDVVEKMANTAQAEMRALLLHLRPAELEGKSLQQGVEYLL 231 Query: 221 PDYCRQNGLKGVKELELQGELSEAIEHQLFLIIQESMANIVKHAGARMVSLSLREVPRQV 280 + ++ G K K LE EL + +E QLF ++QE+++NI++HA A V + + RQV Sbjct: 232 HELEQKQGFKITKRLEPMDELPKGVEDQLFRMLQEAISNILRHAQAHHVEVRFWQTERQV 291 Query: 281 VLSISDDGQGFEHVQHKQGSYGLTTMRERAEKLGGQVEIISRKGAGTTIRVHIP 334 L + DDG GF+ + GSYGL TM ER ++GG ++I+S G GT + IP Sbjct: 292 RLKLIDDGVGFDVQKETHGSYGLQTMHERINEIGGVLDIVSAPGKGTQLEAKIP 345 >DR1556 [T] COG4585 Signal transduction histidine kinase Length = 569 Score = 119 bits (297), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 75/213 (35%), Positives = 119/213 (55%), Gaps = 5/213 (2%) Query: 130 EKAAESAVLEERRRMARDLHDTVSQQLFAIHMSASSLPKVLERN-AEHGK----TVMDQL 184 E+A+ A EER R+AR+LHD+V+Q L+ I + A + L+R+ +E G+ +D Sbjct: 351 ERASVRAGEEERARLARELHDSVAQALYGITLGAKTARATLDRDPSEAGRNRTQASLDYT 410 Query: 185 ITMSQMAQKQMRALIAQLRPVELEGRNLFEALEKWFPDYCRQNGLKGVKELELQGELSEA 244 I +++ +M+AL+ LRP LE L AL + ++GL +L + L+ Sbjct: 411 IRLAEGGVSEMKALLFSLRPDALEEGGLVAALTQHAHALEARHGLTVHADLGHEPRLTPD 470 Query: 245 IEHQLFLIIQESMANIVKHAGARMVSLSLREVPRQVVLSISDDGQGFEHVQHKQGSYGLT 304 + + + QE++ N+VKHA A V LSL E V L++ DDG+GF+ +G+ G Sbjct: 471 AQAAAYRVAQEALHNVVKHARAAQVWLSLHEASGTVTLTVRDDGRGFDPQAQGRGTLGQR 530 Query: 305 TMRERAEKLGGQVEIISRKGAGTTIRVHIPKFL 337 +MRERA GG + + S GAGTT+ + +P L Sbjct: 531 SMRERAAGAGGTLTVDSELGAGTTVTLTLPAAL 563 >DR0577 [T] COG4585 Signal transduction histidine kinase Length = 590 Score = 113 bits (282), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 81/262 (30%), Positives = 129/262 (49%), Gaps = 16/262 (6%) Query: 89 MPESDDQSFTRVYHEFNMMMDTVEN-KMQLLQRLGEQEVIEKEKA------AESAVLEER 141 +P DDQ V + D E ++ LL + Q + A E A+ +ER Sbjct: 331 LPMRDDQGPVGVLVLGDARADAFEGARVPLLAFMAGQATLAVRNARAYLYSEELAISDER 390 Query: 142 RRMARDLHDTVSQQLFAIHMSASSLPKVLERNAEHGKTVMDQLITMSQMAQKQMRALIAQ 201 R+AR++HD V+Q L + + + L + + + + + + + +++R I Sbjct: 391 ARIAREIHDGVAQSLAFAAIKLDLVGRKLRSDPDKSEADVKEASALLREQIREVRRSIFA 450 Query: 202 LRPVELEGRNLFEALEKWFPDYCRQNGLKGVKELELQGE--LSEAIEHQLFLIIQESMAN 259 LRPV+LE L E + ++ D+ QNG++ L+L G+ LS E +F I+QES+ N Sbjct: 451 LRPVDLERYGLLETVRRYVADFGEQNGVR--TNLDLTGDIHLSPGDEAVVFRILQESLNN 508 Query: 260 IVKHAGARMVSLSLREVPRQVVLSISDDGQGFEHVQ-----HKQGSYGLTTMRERAEKLG 314 + KHA AR V++SL +V L + DDGQGF+ G GL M+ER E G Sbjct: 509 VAKHARAREVTVSLHGGANRVTLRVQDDGQGFDPDAVSGRVSSAGGLGLMQMQERLEARG 568 Query: 315 GQVEIISRKGAGTTIRVHIPKF 336 G I+S G GT + +P+ Sbjct: 569 GSYRILSAPGHGTVVEAQLPQL 590 >SP0386 [T] COG4585 Signal transduction histidine kinase Length = 331 Score = 109 bits (273), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 64/191 (33%), Positives = 110/191 (57%), Gaps = 2/191 (1%) Query: 145 ARDLHDTVSQQLFAIHMSASSLPK-VLERNAEHGKTVMDQLITMSQMAQKQMRALIAQLR 203 ARDLHDTVSQ+LFA HM S + + L+ + E +T + + + + AQK +R L+ LR Sbjct: 137 ARDLHDTVSQELFAAHMILSGISQQALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLR 196 Query: 204 PVELEGRNLFEALEKWFPDYCRQNGLKGVKELELQGELSEAIEHQLFLIIQESMANIVKH 263 PVELE ++L E ++ + ++ L+ V + +L + IE +F I+QE ++N ++H Sbjct: 197 PVELEQKSLIEGIQILLKELEDKSDLR-VSLKQNMTKLPKKIEEHIFRILQELISNTLRH 255 Query: 264 AGARMVSLSLREVPRQVVLSISDDGQGFEHVQHKQGSYGLTTMRERAEKLGGQVEIISRK 323 A A + + L + ++ L + D+G GF+ SYGL ++ER E + G V++++ Sbjct: 256 AQASCLDVYLYQTDVELQLKVVDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAP 315 Query: 324 GAGTTIRVHIP 334 G + + IP Sbjct: 316 KQGLAVDIRIP 326 >BS_yhcY_2 [T] COG4585 Signal transduction histidine kinase Length = 182 Score = 104 bits (260), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 0/209 (0%), Positives = 0/209 (0%), Gaps = 9/209 (4%) Query: 134 ESAVLEERRRMARDLHDTVSQQLFAIHMSASSLPKVLERNAEHGKTVMDQLITMSQMAQK 193 E +L + + + + + + L + V + ++ Sbjct: 4 EHVILLNAQGVPTGTLEKYAAHTADTRLHLAF-SSWLFNA-KGQLLVTRRALSKKAWPGV 61 Query: 194 QMRALIAQLRPVELEGRNLFEALEKWFPDYCRQNGLKGVKELELQGELSEAIEHQLFLII 253 ++ + E + Y + + + + Sbjct: 62 WTNSVCGHPQLGESN-EDAVIRRC----RYELGVEITPPESIYPDFRYRATDPSGIVENE 116 Query: 254 QESMANIVKHAGARMVSLSLREVPRQVVLSISDDGQGFEHVQHKQ-GSYGLTTMRERAEK 312 + + ++ + + + + + Sbjct: 117 VCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSPWMVMQATNREARKRLS 176 Query: 313 LGGQVEIISRKGAGTTIRVHIP-KFLQGG 340 Sbjct: 177 205 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,785,134 Number of extensions: 958788 Number of successful extensions: 3050 Number of sequences better than 1.0e-05: 67 Number of HSP's gapped: 2961 Number of HSP's successfully gapped: 67 Length of query: 351 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 244 Effective length of database: 26,037,693 Effective search space: 6353197092 Effective search space used: 6353197092 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)