BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0019.p (474 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value glnA [E] COG0174 Glutamine synthetase 515 e-146 ZglnA [E] COG0174 Glutamine synthetase 515 e-146 ECs4792 [E] COG0174 Glutamine synthetase 515 e-146 Rv2220 [E] COG0174 Glutamine synthetase 513 e-145 MT2278 [E] COG0174 Glutamine synthetase 513 e-145 >glnA [E] COG0174 Glutamine synthetase Length = 211 Score = 515 bits (1326), Expect = e-146, Method: Compositional matrix adjust. Identities = 0/475 (0%), Positives = 0/475 (0%), Gaps = 7/475 (1%) Query: 1 MSVEKVLQTIKENNIEWVDFRFVDLGGRAHHISLPASAVDEETFVNGVAFDGSSITGFRG 60 M+ K L+ + + + + T V+ Sbjct: 1 MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANT 60 Query: 61 IEESDMVMMPDPGTIYIDPFTAHPTLNIMCDIFTPDG-ERYERDPRGIAVKAEEFLQASG 119 + + T + + + P A Sbjct: 61 PAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRK 120 Query: 120 VGTAAFFAPESEFFIFDDVRYESGMNSSSYFVDSEEAVWNTNRKDEGGNLAFKVGVKGGY 179 I D N + + Sbjct: 121 TANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLI 180 Query: 180 VPVAPVDSQQDIRSEMCRLLSEAGLEIERHHHEVATAGQAEINFRFDTLKKTADNLLTYK 239 Sbjct: 181 240 Query: 240 YIVQNTARQYGKVATFMPKPLFGDNGSGMHVHQSIFNGDSPLFYEKGAYANLSEMALNYI 299 Sbjct: 241 - 299 Query: 300 GGILYHAPALIALTNPSTNSFKRLVPGYEAPVNLVFSKGNRSAAVRIPVAAVTPKGCRIE 359 Sbjct: 300 - 358 Query: 360 FRTPDSTANPYLAFSAMLMAGLDGIKKKINPVELGYGPLDKNIYELADEDKEKIRSVPGT 419 Sbjct: 359 ---- 414 Query: 420 LEEALDSLEADYEFLTEGGVFTKDFIDNYIALKRSEAQAVAIRVHPHEYSLYFDV 474 Sbjct: 415 469 >ZglnA [E] COG0174 Glutamine synthetase Length = 252 Score = 515 bits (1326), Expect = e-146, Method: Compositional matrix adjust. Identities = 0/475 (0%), Positives = 0/475 (0%), Gaps = 7/475 (1%) Query: 1 MSVEKVLQTIKENNIEWVDFRFVDLGGRAHHISLPASAVDEETFVNGVAFDGSSITGFRG 60 MS + + + ++ A + G Sbjct: 1 MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITG 60 Query: 61 IEESDMVMMPDPGTIYIDPFTAHPTLNIMCDIFTPDG-ERYERDPRGIAVKAEEFLQASG 119 I+ S ++ + F N + L + Sbjct: 61 IDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLL 120 Query: 120 VGTAAFFAPESEFFIFDDVRYESGMNSSSYFVDSEEAVWNTNRKDEGGNLAFKVGVKGGY 179 + ++ + + D V Sbjct: 121 NPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWR 180 Query: 180 VPVAPVDSQQDIRSEMCRLLSEAGLEIERHHHEVATAGQAEINFRFDTLKKTADNLLTYK 239 + + E + + + + Sbjct: 181 TTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLA 240 Query: 240 YIVQNTARQYGKVATFMPKPLFGDNGSGMHVHQSIFNGDSPLFYEKGAYANLSEMALNYI 299 Sbjct: 241 - 299 Query: 300 GGILYHAPALIALTNPSTNSFKRLVPGYEAPVNLVFSKGNRSAAVRIPVAAVTPKGCRIE 359 Sbjct: 300 - 358 Query: 360 FRTPDSTANPYLAFSAMLMAGLDGIKKKINPVELGYGPLDKNIYELADEDKEKIRSVPGT 419 Sbjct: 359 ---- 414 Query: 420 LEEALDSLEADYEFLTEGGVFTKDFIDNYIALKRSEAQAVAIRVHPHEYSLYFDV 474 Sbjct: 415 469 >ECs4792 [E] COG0174 Glutamine synthetase Length = 252 Score = 515 bits (1326), Expect = e-146, Method: Compositional matrix adjust. Identities = 0/475 (0%), Positives = 0/475 (0%), Gaps = 7/475 (1%) Query: 1 MSVEKVLQTIKENNIEWVDFRFVDLGGRAHHISLPASAVDEETFVNGVAFDGSSITGFRG 60 MS + + + ++ A + G Sbjct: 1 MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITG 60 Query: 61 IEESDMVMMPDPGTIYIDPFTAHPTLNIMCDIFTPDG-ERYERDPRGIAVKAEEFLQASG 119 I+ S ++ + F N + L + Sbjct: 61 IDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLL 120 Query: 120 VGTAAFFAPESEFFIFDDVRYESGMNSSSYFVDSEEAVWNTNRKDEGGNLAFKVGVKGGY 179 + ++ + + D V Sbjct: 121 NPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWR 180 Query: 180 VPVAPVDSQQDIRSEMCRLLSEAGLEIERHHHEVATAGQAEINFRFDTLKKTADNLLTYK 239 + + E + + + + Sbjct: 181 TTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLA 240 Query: 240 YIVQNTARQYGKVATFMPKPLFGDNGSGMHVHQSIFNGDSPLFYEKGAYANLSEMALNYI 299 Sbjct: 241 - 299 Query: 300 GGILYHAPALIALTNPSTNSFKRLVPGYEAPVNLVFSKGNRSAAVRIPVAAVTPKGCRIE 359 Sbjct: 300 - 358 Query: 360 FRTPDSTANPYLAFSAMLMAGLDGIKKKINPVELGYGPLDKNIYELADEDKEKIRSVPGT 419 Sbjct: 359 ---- 414 Query: 420 LEEALDSLEADYEFLTEGGVFTKDFIDNYIALKRSEAQAVAIRVHPHEYSLYFDV 474 Sbjct: 415 469 >Rv2220 [E] COG0174 Glutamine synthetase Length = 252 Score = 513 bits (1321), Expect = e-145, Method: Compositional matrix adjust. Identities = 0/476 (0%), Positives = 0/476 (0%), Gaps = 9/476 (1%) Query: 4 EKVLQTIKENNIEWVDFRFVDLGGRAHHISLPASAVDEETFVNGVAFDGSSITGFRGIEE 63 L E + V+ +++ T + + + ITG Sbjct: 7 SLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPA 66 Query: 64 SDMVMMPDPGTIYIDPFTAHPTLNIMCDIFTP-DGERYERDPRGIAVKAEEFLQASGVGT 122 + + L Sbjct: 67 MIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVL 126 Query: 123 AAFFAPESEFFIFDDVRYESGMNSSSYFVDSEEAVWNT---NRKDEGGNLAFKVGVKGGY 179 A +R + + A + + G + Sbjct: 127 AVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQ 186 Query: 180 VPVAPVDSQQDIRSEMCRLLSEAGLEIERHHHEVATAGQAEINFRFDTLKKTADNLLTYK 239 +P + + L + ++ + E + + + Sbjct: 187 MPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLAFPRLFI 246 Query: 240 YIVQNTARQYGKVATFMPKPLFGDNGSGMHVHQSIFNGDSPLFYEKGAYANLSEMALNYI 299 Sbjct: 247 306 Query: 300 GGILYHAPALIALTNPSTNSFKRLVPGYEAPVNLVFSKGNRSAAVRIPVAAVTPKGCRIE 359 Sbjct: 307 366 Query: 360 FRTPDSTANPYLAFSAMLMAGLDGIKKKINPVELGYGPLDKNIYELADEDKEKIRSVPGT 419 Sbjct: 367 ---- 422 Query: 420 LEEALDSLEADYEFLTEGGVFTKDFIDNYIALKR-SEAQAVAIRVHPHEYSLYFDV 474 Sbjct: 423 478 >MT2278 [E] COG0174 Glutamine synthetase Length = 252 Score = 513 bits (1321), Expect = e-145, Method: Compositional matrix adjust. Identities = 0/476 (0%), Positives = 0/476 (0%), Gaps = 9/476 (1%) Query: 4 EKVLQTIKENNIEWVDFRFVDLGGRAHHISLPASAVDEETFVNGVAFDGSSITGFRGIEE 63 L E + V+ +++ T + + + ITG Sbjct: 7 SLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSPA 66 Query: 64 SDMVMMPDPGTIYIDPFTAHPTLNIMCDIFTP-DGERYERDPRGIAVKAEEFLQASGVGT 122 + + L Sbjct: 67 MIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGVL 126 Query: 123 AAFFAPESEFFIFDDVRYESGMNSSSYFVDSEEAVWNT---NRKDEGGNLAFKVGVKGGY 179 A +R + + A + + G + Sbjct: 127 AVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYHQ 186 Query: 180 VPVAPVDSQQDIRSEMCRLLSEAGLEIERHHHEVATAGQAEINFRFDTLKKTADNLLTYK 239 +P + + L + ++ + E + + + Sbjct: 187 MPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLAFPRLFI 246 Query: 240 YIVQNTARQYGKVATFMPKPLFGDNGSGMHVHQSIFNGDSPLFYEKGAYANLSEMALNYI 299 Sbjct: 247 306 Query: 300 GGILYHAPALIALTNPSTNSFKRLVPGYEAPVNLVFSKGNRSAAVRIPVAAVTPKGCRIE 359 Sbjct: 307 366 Query: 360 FRTPDSTANPYLAFSAMLMAGLDGIKKKINPVELGYGPLDKNIYELADEDKEKIRSVPGT 419 Sbjct: 367 ---- 422 Query: 420 LEEALDSLEADYEFLTEGGVFTKDFIDNYIALKR-SEAQAVAIRVHPHEYSLYFDV 474 Sbjct: 423 478 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 36,825,484 Number of extensions: 1585826 Number of successful extensions: 2740 Number of sequences better than 1.0e-05: 67 Number of HSP's gapped: 2509 Number of HSP's successfully gapped: 72 Length of query: 474 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 364 Effective length of database: 25,691,691 Effective search space: 9351775524 Effective search space used: 9351775524 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)