BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0017.p (183 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ydjF [KT] COG1842 Phage shock protein A (IM30), suppresses si... 137 1e-32 Z2808 [KG] COG1349 Transcriptional regulators of sugar metabolism 136 2e-32 ECs2479 [KG] COG1349 Transcriptional regulators of sugar metabolism 136 2e-32 BH1553 [KG] COG1349 Transcriptional regulators of sugar metabolism 108 3e-24 YPO3327 [KG] COG1349 Transcriptional regulators of sugar metabolism 99 4e-21 >BS_ydjF [KT] COG1842 Phage shock protein A (IM30), suppresses sigma54-dependent transcription Length = 217 Score = 137 bits (344), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 0/175 (0%), Positives = 0/175 (0%), Gaps = 1/175 (0%) Query: 4 EAKRAIAESVLDLINDGDSLMTDTSSTAFEALKKITEAKKNLTIITNSLVVLSEFQHSGH 63 + + L L S+ + + +V + + Sbjct: 78 KKITVVPILRAGLGMMDGVLENVPSARISVVGM-YRNEETLEPVPYFQKLVSNIDERMAL 136 Query: 64 KLISTGGVLGAETNSFVGPSASHTIQRYNVDAALFSCKALSMTGGLSDSNEAESELKILM 123 + G+ + + + ++ D + + + Sbjct: 137 IVDPMLATGGSVIATIDLLKKAGCSSIKVLVLVAAPEGIAALEKAHPDVELYTASIDQGL 196 Query: 124 RQQASKAVLLADHSKFDRIAFVRLFSFDEIDYIVTDGKPSDEWISFLNKYQVSLL 178 Sbjct: 197 251 >Z2808 [KG] COG1349 Transcriptional regulators of sugar metabolism Length = 252 Score = 136 bits (343), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 74/175 (42%), Positives = 103/175 (58%), Gaps = 1/175 (0%) Query: 4 EAKRAIAESVLDLINDGDSLMTDTSSTAFEALKKITEAKKNLTIITNSLVVLSEFQHSGH 63 E K+ IA L I++ ++ D+SST E LK + + + +LT++TNS + S Sbjct: 78 EEKQLIARKALPFIDNKTTMAADSSSTVMELLK-LLQDRSDLTLLTNSAEAIHVLAQSEI 136 Query: 64 KLISTGGVLGAETNSFVGPSASHTIQRYNVDAALFSCKALSMTGGLSDSNEAESELKILM 123 K++STGG L T S G I RY+VD + SCK L + G DSNEAE+E+K M Sbjct: 137 KVVSTGGELNKNTLSLQGRITKEIISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTM 196 Query: 124 RQQASKAVLLADHSKFDRIAFVRLFSFDEIDYIVTDGKPSDEWISFLNKYQVSLL 178 +QA++ LL DHSKFDR AFV+L F I+YI+TD P EWI+F + L+ Sbjct: 197 IRQATEVALLVDHSKFDRKAFVQLADFSHINYIITDKSPGAEWIAFCKDNNIQLV 251 >ECs2479 [KG] COG1349 Transcriptional regulators of sugar metabolism Length = 252 Score = 136 bits (343), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 74/175 (42%), Positives = 103/175 (58%), Gaps = 1/175 (0%) Query: 4 EAKRAIAESVLDLINDGDSLMTDTSSTAFEALKKITEAKKNLTIITNSLVVLSEFQHSGH 63 E K+ IA L I++ ++ D+SST E LK + + + +LT++TNS + S Sbjct: 78 EEKQLIARKALPFIDNKTTMAADSSSTVMELLK-LLQDRSDLTLLTNSAEAIHVLAQSEI 136 Query: 64 KLISTGGVLGAETNSFVGPSASHTIQRYNVDAALFSCKALSMTGGLSDSNEAESELKILM 123 K++STGG L T S G I RY+VD + SCK L + G DSNEAE+E+K M Sbjct: 137 KVVSTGGELNKNTLSLQGRITKEIISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTM 196 Query: 124 RQQASKAVLLADHSKFDRIAFVRLFSFDEIDYIVTDGKPSDEWISFLNKYQVSLL 178 +QA++ LL DHSKFDR AFV+L F I+YI+TD P EWI+F + L+ Sbjct: 197 IRQATEVALLVDHSKFDRKAFVQLADFSHINYIITDKSPGAEWIAFCKDNNIQLV 251 >BH1553 [KG] COG1349 Transcriptional regulators of sugar metabolism Length = 251 Score = 108 bits (271), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 61/179 (34%), Positives = 104/179 (58%), Gaps = 3/179 (1%) Query: 2 NIEAKRAIAESVLDLINDGDSLMTDTSSTAFEALKKITEAKKNLTIITNSLVVLSEF-QH 60 N+ K+A+A + + D ++ D S+TA+ K++ + LT++TNS+ V E + Sbjct: 74 NVNEKQAVAAQAVTYVTTNDRIILDASTTAWYMAKRLPDVP--LTVLTNSMKVAMELAKR 131 Query: 61 SGHKLISTGGVLGAETNSFVGPSASHTIQRYNVDAALFSCKALSMTGGLSDSNEAESELK 120 +I+ GG L ++ SFVGP + T++ Y+V+ A SC+ + G+SDSNE ++ +K Sbjct: 132 EKITVITVGGTLLRKSLSFVGPQTNQTLELYHVNKAFISCQGVHAKRGISDSNEMQALVK 191 Query: 121 ILMRQQASKAVLLADHSKFDRIAFVRLFSFDEIDYIVTDGKPSDEWISFLNKYQVSLLH 179 M + LLADH+KF + AF R+ S + ID I+TD K S+E IS + ++ ++ Sbjct: 192 QKMIDISDHVFLLADHTKFGKQAFSRITSMETIDTIITDSKTSEEQISDFQELEMDVIR 250 >YPO3327 [KG] COG1349 Transcriptional regulators of sugar metabolism Length = 269 Score = 99.0 bits (245), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 50/166 (30%), Positives = 95/166 (57%), Gaps = 3/166 (1%) Query: 4 EAKRAIAESVLDLINDGDSLMTDTSSTAFEALKKITEAKKNLTIITNSLVVLSEFQHSGH 63 + K IA++ L I GD ++ D SS+ + ++I + + +T++TNS+ ++ Sbjct: 94 DTKMKIAKAALQFIRPGDCILLDNSSSCWFLARQIPDIE--ITVVTNSVKIIQALACRDK 151 Query: 64 -KLISTGGVLGAETNSFVGPSASHTIQRYNVDAALFSCKALSMTGGLSDSNEAESELKIL 122 ++I GG A + F GP A + I+ + ++ FSC+ ++ G+ D N+ ++LK + Sbjct: 152 LRIIGIGGEYSARHDDFHGPVAENAIRNFQINTFFFSCQGINQDSGVRDGNDINAKLKQV 211 Query: 123 MRQQASKAVLLADHSKFDRIAFVRLFSFDEIDYIVTDGKPSDEWIS 168 M Q + + +LLAD SKFD+ AF ++ + DE+D ++T+ S + S Sbjct: 212 MLQVSGQKILLADSSKFDQYAFCKICTLDEVDILITNRLSSQNYRS 257 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.129 0.350 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,837,056 Number of extensions: 381780 Number of successful extensions: 1571 Number of sequences better than 1.0e-05: 112 Number of HSP's gapped: 1515 Number of HSP's successfully gapped: 112 Length of query: 183 Length of database: 38,378,431 Length adjustment: 100 Effective length of query: 83 Effective length of database: 26,845,031 Effective search space: 2228137573 Effective search space used: 2228137573 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 112 (47.8 bits)