BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0016.p (154 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0615 [K] COG1846 Transcriptional regulators 62 3e-10 Ta0411 [K] COG1846 Transcriptional regulators 53 1e-07 >lin0615 [K] COG1846 Transcriptional regulators Length = 153 Score = 62.0 bits (149), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 36/134 (26%), Positives = 66/134 (49%), Gaps = 1/134 (0%) Query: 12 LMEAFQQFSKINWQKNNPSLLKPSEIRVLITIRLGTEKSGKPVLTVSDISKMHKVTSPTI 71 +++AF F + SE R + + G + G P + VSD+ M +++ P++ Sbjct: 12 VIKAFMNFKHAEIKSFQIPGYSKSETRFIFILSRGLKSRG-PKIRVSDLGHMLRISKPSV 70 Query: 72 TQMVNSLLAQGYVVRTSDAQDKRVSGIALTDKGVRLXXXXXXXXXXTFKGMIDYLGKERS 131 TQM+ SL +G + R + +DKR + LT+ G + +F+ M ++LG++ Sbjct: 71 TQMIQSLEGKGLIKRVQNPEDKRSMYVELTEVGAGVSKKMFDEFQASFEDMQEFLGEDDM 130 Query: 132 RALIELLNGVYHYF 145 + LI LL + Y Sbjct: 131 KKLITLLEKLTDYL 144 >Ta0411 [K] COG1846 Transcriptional regulators Length = 153 Score = 53.1 bits (126), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 33/105 (31%), Positives = 58/105 (55%), Gaps = 14/105 (13%) Query: 7 DKIDELMEAFQQFSKI---NWQKNNPSL----LKPSEIRVLITIRLGTEKSGKPVLTVSD 59 + D LME + F+K+ ++ SL LKP E+R+L + + V+ Sbjct: 4 EDCDALMEIWHHFAKVYVLGKRRMEDSLQITQLKPIEVRILYYL-------SEENFNVNR 56 Query: 60 ISKMHKVTSPTITQMVNSLLAQGYVVRTSDAQDKRVSGIALTDKG 104 +++M+ VT IT +++ L +GY+VR+ +A D+RV + +TDKG Sbjct: 57 LAEMNFVTPAWITGVLDDLENRGYIVRSRNASDRRVVNVEITDKG 101 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.134 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 9,835,957 Number of extensions: 310530 Number of successful extensions: 812 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 818 Number of HSP's successfully gapped: 4 Length of query: 154 Length of database: 38,378,431 Length adjustment: 97 Effective length of query: 57 Effective length of database: 27,191,033 Effective search space: 1549888881 Effective search space used: 1549888881 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 111 (47.4 bits)