BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0014.p (275 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BMEII0638 [R] COG0596 Predicted hydrolases or acyltransferases (... 57 4e-08 >BMEII0638 [R] COG0596 Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily) Length = 267 Score = 56.6 bits (135), Expect = 4e-08, Method: Compositional matrix adjust. Identities = 64/244 (26%), Positives = 98/244 (40%), Gaps = 16/244 (6%) Query: 22 VVFIHDHLTSHHLFEPQIEYFRDRVQVIVMDLRGNGLS--GKMDVEVHRILDTQCEDXXX 79 +VFI+ T ++ V +V D RG+GLS GK + + +D Sbjct: 25 LVFINSLGTDFRIWNKVRARLGHDVSTLVYDKRGHGLSDIGKTPYTIELL----AQDLIA 80 Query: 80 XXXXXXXXXXXXVASSGGGVLAQKFAVQNPELVRALVLVDNCSS-GHDSTINNRIWGIVE 138 S GG++AQ P+LV LVL + G N RI I++ Sbjct: 81 LLDRLSIHKAVICGLSVGGLIAQGLYAARPDLVAGLVLSNTAHKIGTPEMWNARIDAIMQ 140 Query: 139 R--CSWMSYYLPPELLLRSLRIAYNKWLPAYHILRNELLHKRPTEGIKQRIA-LRQIDIL 195 S + +P R AY + A + + ++P EG A LR D Sbjct: 141 NGLASILDATMP-----RWFTAAYRRPDNAAYQAYCNMFTRQPLEGYAATCAALRDADFT 195 Query: 196 AYAAKLQVPVLCVTGSQNEWRLAQASKN-ASMLPSAQLVVLDDAMYPSHLCQPQHFNRLL 254 A A K+ VPV CV G Q+ + AS++P A + ++ + + QP + LL Sbjct: 196 AAAHKISVPVRCVAGDQDGSTPPTLVQELASLIPGAVFSQIANSGHIPCVEQPDAYTALL 255 Query: 255 LNFL 258 +FL Sbjct: 256 RDFL 259 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.139 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,308,610 Number of extensions: 645844 Number of successful extensions: 983 Number of sequences better than 1.0e-05: 1 Number of HSP's gapped: 991 Number of HSP's successfully gapped: 1 Length of query: 275 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 170 Effective length of database: 26,268,361 Effective search space: 4465621370 Effective search space used: 4465621370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 115 (48.9 bits)