BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0541.0012.p
         (97 letters)

Database: COG 
           115,334 sequences; 38,378,431 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SA0808 [P] COG2212 Multisubunit Na+/H+ antiporter, MnhF subunit        63   9e-11
PAB3177 [P] COG2212 Multisubunit Na+/H+ antiporter, MnhF subunit       53   1e-07
AGc896 [P] COG2212 Multisubunit Na+/H+ antiporter, MnhF subunit        50   1e-06
SMc03183 [P] COG2212 Multisubunit Na+/H+ antiporter, MnhF subunit      49   2e-06

>SA0808 [P] COG2212 Multisubunit Na+/H+ antiporter, MnhF subunit
          Length = 97

 Score = 63.2 bits (152), Expect = 9e-11,   Method: Compositional matrix adjust.
 Identities = 28/74 (37%), Positives = 50/74 (67%)

Query: 23 RLIQGPTRSDRVAALDTIGIHLLAVIAVLGMQQRTEDYFDMVLVIGILTFIGTAALARYI 82
          R++ GP+ +DRV ALD IG+ L+AVIA+  +    +    ++++IGIL F+GTA  ++++
Sbjct: 24 RVVLGPSLADRVVALDAIGLQLMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFM 83

Query: 83 ERDAVMEHGGDKLD 96
          ++  V+EH  +  D
Sbjct: 84 DKGKVIEHDQNHTD 97


>PAB3177 [P] COG2212 Multisubunit Na+/H+ antiporter, MnhF subunit
          Length = 88

 Score = 52.8 bits (125), Expect = 1e-07,   Method: Compositional matrix adjust.
 Identities = 26/61 (42%), Positives = 40/61 (65%)

Query: 23 RLIQGPTRSDRVAALDTIGIHLLAVIAVLGMQQRTEDYFDMVLVIGILTFIGTAALARYI 82
          RLI GPT SDRV ALDT+   ++A + +LG       Y D+ +V  +L++IGT  +A+Y+
Sbjct: 24 RLILGPTTSDRVVALDTLNTLVVAAMLLLGAYYERAIYIDIAIVYALLSYIGTLIIAKYL 83

Query: 83 E 83
          +
Sbjct: 84 Q 84


>AGc896 [P] COG2212 Multisubunit Na+/H+ antiporter, MnhF subunit
          Length = 177

 Score = 49.7 bits (117), Expect = 1e-06,   Method: Compositional matrix adjust.
 Identities = 2/68 (2%), Positives = 8/68 (11%)

Query: 22 YRLIQGPTRSDRVAALDTIGIHLLAVIAVLGMQQRTEDYFDMVLVIGILTFIGTAALARY 81
           R         +   +       +           ++                       
Sbjct: 26 PRPFVKTDWIFQAFKVGCPIRRSMDQSLFAAPHGFSQRITSFFACACQGIHQMPLIHFFV 85

Query: 82 IERDAVME 89
          +   A   
Sbjct: 86 LIVYAHHL 93


>SMc03183 [P] COG2212 Multisubunit Na+/H+ antiporter, MnhF subunit
          Length = 93

 Score = 48.5 bits (114), Expect = 2e-06,   Method: Compositional matrix adjust.
 Identities = 22/68 (32%), Positives = 41/68 (60%)

Query: 22 YRLIQGPTRSDRVAALDTIGIHLLAVIAVLGMQQRTEDYFDMVLVIGILTFIGTAALARY 81
          YR+ +GP   DR+  LDT+ I+ + ++   G++     YF+  L+I ++ F  + ALA++
Sbjct: 26 YRMARGPRAQDRILGLDTLYINAMLMLITFGIRTANTVYFETALIIAVIGFASSIALAKF 85

Query: 82 IERDAVME 89
          + R  V+E
Sbjct: 86 LMRGEVIE 93


  Database: COG
    Posted date:  Aug 3, 2010  9:58 AM
  Number of letters in database: 38,378,431
  Number of sequences in database:  115,334
  
Lambda     K      H
   0.328    0.144    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 115334
Number of Hits to DB: 5,599,261
Number of extensions: 177207
Number of successful extensions: 437
Number of sequences better than 1.0e-05: 9
Number of HSP's gapped: 437
Number of HSP's successfully gapped: 9
Length of query: 97
Length of database: 38,378,431
Length adjustment: 66
Effective length of query: 31
Effective length of database: 30,766,387
Effective search space: 953757997
Effective search space used: 953757997
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 109 (46.6 bits)