BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0011.p (161 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SA0809 [P] COG1863 Multisubunit Na+/H+ antiporter, MnhE subunit 124 5e-29 SMc00055 [P] COG1863 Multisubunit Na+/H+ antiporter, MnhE subunit 114 9e-26 AGc1664 [P] COG1863 Multisubunit Na+/H+ antiporter, MnhE subunit 101 6e-22 SA0582 [P] COG1863 Multisubunit Na+/H+ antiporter, MnhE subunit 100 7e-22 DR0748a [P] COG1863 Multisubunit Na+/H+ antiporter, MnhE subunit 70 2e-12 >SA0809 [P] COG1863 Multisubunit Na+/H+ antiporter, MnhE subunit Length = 159 Score = 124 bits (312), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 59/156 (37%), Positives = 96/156 (61%) Query: 3 MAVQILLNLMTAFLWMMLSGDGSGSGFVIGYLLGCGILLVLRRFLPEPLYLKRVWSIVKL 62 MAVQ++LN + A W+ ++ + + FV+G++ G ++ +L R LP Y+ ++ I+KL Sbjct: 1 MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRVLPGRFYVITLYRIIKL 60 Query: 63 LALFLRELVWSSFAVIRQILSPKLAVRPGIFAYETALRSDFEVTLLSCLICLTPGTLTLE 122 + +FL EL+ ++F V++ I+ P + PG F Y T L+ D+++ LLS LI LTPGT+ L Sbjct: 61 VIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQIVLLSNLITLTPGTVVLG 120 Query: 123 VSGSGRTLYIHAMDIEDAGQLSRQIEGTFEKAIMEV 158 VS +YIHA+D Q I+ + EK + EV Sbjct: 121 VSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREV 156 >SMc00055 [P] COG1863 Multisubunit Na+/H+ antiporter, MnhE subunit Length = 158 Score = 114 bits (284), Expect = 9e-26, Method: Compositional matrix adjust. Identities = 64/152 (42%), Positives = 92/152 (60%) Query: 7 ILLNLMTAFLWMMLSGDGSGSGFVIGYLLGCGILLVLRRFLPEPLYLKRVWSIVKLLALF 66 +L NL+ +W +S + + ++G+ G L ++RR L Y R ++ L +LF Sbjct: 4 LLTNLVFTLIWGAISASFTPANLLLGFAAGALSLWLIRRELRPVAYPLRPLRLLLLASLF 63 Query: 67 LRELVWSSFAVIRQILSPKLAVRPGIFAYETALRSDFEVTLLSCLICLTPGTLTLEVSGS 126 +EL S+ V +L +A++PGIFAY ALRSDFE+TLL+ LI LTPGTL+++VS Sbjct: 64 FKELAISATKVAVLVLRSNMALKPGIFAYPLALRSDFEITLLANLITLTPGTLSVDVSDD 123 Query: 127 GRTLYIHAMDIEDAGQLSRQIEGTFEKAIMEV 158 TLY+HA+D D G L R I G FE+ I E Sbjct: 124 RNTLYVHALDCADPGALRRDIAGGFERRIREA 155 >AGc1664 [P] COG1863 Multisubunit Na+/H+ antiporter, MnhE subunit Length = 159 Score = 101 bits (251), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 54/143 (37%), Positives = 86/143 (60%), Gaps = 1/143 (0%) Query: 16 LWMMLSGDGSGSGFVIGYLLGCGILLVLRRFLPE-PLYLKRVWSIVKLLALFLRELVWSS 74 +W+ ++G + + V ++ L ++R LP + R++ ++ L+ LF +EL S+ Sbjct: 13 VWLAVTGSLTLANIVFALIVSTLALGLIRHQLPGGRSHWLRLFRVLSLVLLFFKELALSA 72 Query: 75 FAVIRQILSPKLAVRPGIFAYETALRSDFEVTLLSCLICLTPGTLTLEVSGSGRTLYIHA 134 + V + PKL V+PGIFAY + +DFE+TLL+ LI LTPGTL+++VS +TLY+HA Sbjct: 73 WKVAVLVTRPKLDVQPGIFAYPLTVTTDFEITLLANLITLTPGTLSVDVSADRKTLYVHA 132 Query: 135 MDIEDAGQLSRQIEGTFEKAIME 157 +D D I FE+ IME Sbjct: 133 IDCSDIEATKNDIRNGFERKIME 155 >SA0582 [P] COG1863 Multisubunit Na+/H+ antiporter, MnhE subunit Length = 160 Score = 100 bits (250), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 55/159 (34%), Positives = 97/159 (61%), Gaps = 5/159 (3%) Query: 6 QILLNLMTAFLWMMLSGDGSG--SGFVIGYLLGCGILLVLRRFLPEPLYLKRVWSIVKLL 63 QI+LN++ AFLW++ + S F GYL+G ++ +L RF + Y++++W +K L Sbjct: 3 QIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHRFFSDDFYVRKIWVAIKFL 62 Query: 64 ALFLRELVWSSFAVIRQILSPKLAVRPGIFAYETALRSDFEVTLLSCLICLTPGTLTLEV 123 ++L +L+ SS + I IL + PG+ +YET L SD+ +T L+ LI +TPG+ + + Sbjct: 63 GVYLYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDWAITFLTILIIITPGSTVIRI 122 Query: 124 SGSGRTLYIHAMDIEDAGQLS--RQIEGTFEKAIMEVTR 160 S + +IH++D+ + + S R I+ +E I+EV+R Sbjct: 123 SQDSKKFFIHSIDVSEKEKDSLLRSIKH-YEDLILEVSR 160 >DR0748a [P] COG1863 Multisubunit Na+/H+ antiporter, MnhE subunit Length = 246 Score = 69.7 bits (169), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 5/140 (3%), Positives = 23/140 (16%) Query: 9 LNLMTAFLWMMLSGDGSGSGFVIGYLLGCGILLVLRRFLPEPLYLKRVWSIVKLLALFLR 68 L + + + + ++ + + Sbjct: 8 PVLEALPAGRVAEVVLARGVEDAFARQVQDLARDAGVRVRWLPRIELDQLVGTTQHQGVV 67 Query: 69 ELVWSSFAVIRQILSPKLAVRPGIFAYETALRSDFEVTLLSCLICLTPGTLTLEVSGSGR 128 V + + + + V R Sbjct: 68 AEVEELQWATVDDILDRAEASGAPLLMVLLDGVTDPRNFGAIIRSAEVLGAHGVVVEERR 127 Query: 129 TLYIHAMDIEDAGQLSRQIE 148 + + + + A + + Sbjct: 128 SAPLSPVVAKTAAGATAYLP 147 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.329 0.143 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,165,277 Number of extensions: 387493 Number of successful extensions: 1080 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 1075 Number of HSP's successfully gapped: 16 Length of query: 161 Length of database: 38,378,431 Length adjustment: 98 Effective length of query: 63 Effective length of database: 27,075,699 Effective search space: 1705769037 Effective search space used: 1705769037 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 111 (47.4 bits)