BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0541.0003.p (377 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM4108 [C] COG0371 Glycerol dehydrogenase and related enzymes 307 2e-83 gldA [C] COG0371 Glycerol dehydrogenase and related enzymes 306 4e-83 ZgldA [C] COG0371 Glycerol dehydrogenase and related enzymes 306 4e-83 ECs4874 [C] COG0371 Glycerol dehydrogenase and related enzymes 305 9e-83 >STM4108 [C] COG0371 Glycerol dehydrogenase and related enzymes Length = 367 Score = 307 bits (786), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 165/374 (44%), Positives = 227/374 (60%), Gaps = 14/374 (3%) Query: 1 MRKVFISPTKYIQGEDELLNLGYFVKTFGDSALLIAHPDDVQRVKAKLDATSSKFGITLI 60 M ++ SP KYIQG + + LG ++K ++ L++ + + L + + G+++ Sbjct: 1 MDRIIQSPGKYIQGANVIARLGDYLKPMANNWLVVGDKFVLGFAEETLRKSLTDAGLSVE 60 Query: 61 TGNFQGECSRQEVARLQALAREHACACTIGLGGGKAIDTAKCVAE--GDALIIVPTIAAT 118 F GECS+ E+ RL+A+A + C +G+GGGK +DTAK +A + I PTIA+T Sbjct: 61 IAPFGGECSQNEIDRLRAVAEKSQCGAVLGIGGGKTLDTAKALAHFMNVPVAIAPTIAST 120 Query: 119 DAPTSHSAVLYTPEGAFDDYAYFKQSPSVVMIDTTIIAEAPTRFLVSGMGDALSTYFEAR 178 DAP S +V+YT G FD Y +P++V++DT I+A AP R L +G+GDAL+T+FEAR Sbjct: 121 DAPCSALSVIYTDAGEFDRYLLLPHNPNMVIVDTQIVAGAPARLLAAGIGDALATWFEAR 180 Query: 179 ATARSFSNVNAGLPCGVREGVSQEAKGTKAALALARVCYETLLEDGVKAKLACDQNVVTP 238 A +RS + AG C T+AALALA +CY TL+E+G KA LA +Q+VVTP Sbjct: 181 ACSRSGATTMAGGKC------------TQAALALAELCYNTLIEEGEKAMLAAEQHVVTP 228 Query: 239 ALENIIETNIXXXXXXXXXXXXXXXHAIHNGLTVLEGSHHYYHGEKVAFSTLAQLVLENA 298 ALE +IE N HAIHNGLT + +HHYYHGEKVAF TL QLVLENA Sbjct: 229 ALERVIEANTYLSGVGFESGGLAAAHAIHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENA 288 Query: 299 DSREIQEVLAFCASVGLPVCLADIGVHSITREELLEVARKACIPEESIHSMPFPVTPEAV 358 EI+ V A C SVGLP+ LA + + ++ VA +C E+IH+MP TP+ V Sbjct: 289 PVEEIETVAALCHSVGLPITLAQLDIKQDIPAKMRTVAEASCAEGETIHNMPGGATPDEV 348 Query: 359 AAAITVADQLGHAF 372 AA+ VADQ G F Sbjct: 349 YAALLVADQYGQRF 362 >gldA [C] COG0371 Glycerol dehydrogenase and related enzymes Length = 380 Score = 306 bits (784), Expect = 4e-83, Method: Compositional matrix adjust. Identities = 165/374 (44%), Positives = 225/374 (60%), Gaps = 14/374 (3%) Query: 1 MRKVFISPTKYIQGEDELLNLGYFVKTFGDSALLIAHPDDVQRVKAKLDATSSKFGITLI 60 M ++ SP KYIQG D + LG ++K + L++ + ++ ++ + G+ + Sbjct: 14 MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFAQSTVEKSFKDAGLVVE 73 Query: 61 TGNFQGECSRQEVARLQALAREHACACTIGLGGGKAIDTAKCVAE--GDALIIVPTIAAT 118 F GECS+ E+ RL+ +A C +G+GGGK +DTAK +A G + I PTIA+T Sbjct: 74 IAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKALAHFMGVPVAIAPTIAST 133 Query: 119 DAPTSHSAVLYTPEGAFDDYAYFKQSPSVVMIDTTIIAEAPTRFLVSGMGDALSTYFEAR 178 DAP S +V+YT EG FD Y +P++V++DT I+A AP R L +G+GDAL+T+FEAR Sbjct: 134 DAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGAPARLLAAGIGDALATWFEAR 193 Query: 179 ATARSFSNVNAGLPCGVREGVSQEAKGTKAALALARVCYETLLEDGVKAKLACDQNVVTP 238 A +RS + AG C T+AALALA +CY TLLE+G KA LA +Q+VVTP Sbjct: 194 ACSRSGATTMAGGKC------------TQAALALAELCYNTLLEEGEKAMLAAEQHVVTP 241 Query: 239 ALENIIETNIXXXXXXXXXXXXXXXHAIHNGLTVLEGSHHYYHGEKVAFSTLAQLVLENA 298 ALE +IE N HA+HNGLT + +HHYYHGEKVAF TL QLVLENA Sbjct: 242 ALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENA 301 Query: 299 DSREIQEVLAFCASVGLPVCLADIGVHSITREELLEVARKACIPEESIHSMPFPVTPEAV 358 EI+ V A +VGLP+ LA + + ++ VA AC E+IH+MP TP+ V Sbjct: 302 PVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGETIHNMPGGATPDQV 361 Query: 359 AAAITVADQLGHAF 372 AA+ VADQ G F Sbjct: 362 YAALLVADQYGQRF 375 >ZgldA [C] COG0371 Glycerol dehydrogenase and related enzymes Length = 380 Score = 306 bits (784), Expect = 4e-83, Method: Compositional matrix adjust. Identities = 164/374 (43%), Positives = 225/374 (60%), Gaps = 14/374 (3%) Query: 1 MRKVFISPTKYIQGEDELLNLGYFVKTFGDSALLIAHPDDVQRVKAKLDATSSKFGITLI 60 M ++ SP KYIQG D + LG ++K + L++ + ++ ++ + G+ + Sbjct: 14 MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFAQSTVEKSFKDAGLVVE 73 Query: 61 TGNFQGECSRQEVARLQALAREHACACTIGLGGGKAIDTAKCVAE--GDALIIVPTIAAT 118 F GECS+ E+ RL+ +A C +G+GGGK +DTAK +A G + I PTIA+T Sbjct: 74 IAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKALAHFMGVPVAIAPTIAST 133 Query: 119 DAPTSHSAVLYTPEGAFDDYAYFKQSPSVVMIDTTIIAEAPTRFLVSGMGDALSTYFEAR 178 DAP S +V+YT EG FD Y +P++V++DT I+A AP R L +G+GDAL+T+FEAR Sbjct: 134 DAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGAPARLLAAGIGDALATWFEAR 193 Query: 179 ATARSFSNVNAGLPCGVREGVSQEAKGTKAALALARVCYETLLEDGVKAKLACDQNVVTP 238 A +RS + AG C T+AALALA +CY TLLE+G KA LA +Q+VVTP Sbjct: 194 ACSRSGATTMAGGKC------------TQAALALAELCYNTLLEEGEKAMLAAEQHVVTP 241 Query: 239 ALENIIETNIXXXXXXXXXXXXXXXHAIHNGLTVLEGSHHYYHGEKVAFSTLAQLVLENA 298 ALE +IE N HA+HNGLT + +HHYYHGEKVAF TL QLVLEN+ Sbjct: 242 ALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENS 301 Query: 299 DSREIQEVLAFCASVGLPVCLADIGVHSITREELLEVARKACIPEESIHSMPFPVTPEAV 358 EI+ V A +VGLP+ LA + + ++ VA AC E+IH+MP TP+ V Sbjct: 302 PVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGETIHNMPGGATPDQV 361 Query: 359 AAAITVADQLGHAF 372 AA+ VADQ G F Sbjct: 362 YAALLVADQYGQRF 375 Score = 305 bits (781), Expect = 9e-83, Method: Compositional matrix adjust. Identities = 164/374 (43%), Positives = 225/374 (60%), Gaps = 14/374 (3%) Query: 1 MRKVFISPTKYIQGEDELLNLGYFVKTFGDSALLIAHPDDVQRVKAKLDATSSKFGITLI 60 M ++ SP KYIQG D + LG ++K + L++ + ++ ++ + G+ + Sbjct: 14 MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFAQSTVEKSFKDAGLVVE 73 Query: 61 TGNFQGECSRQEVARLQALAREHACACTIGLGGGKAIDTAKCVAE--GDALIIVPTIAAT 118 F GECS+ E+ RL+ +A C +G+GGGK +DTAK +A G + I PTIA+T Sbjct: 74 IAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKALAHFMGVPVAIAPTIAST 133 Query: 119 DAPTSHSAVLYTPEGAFDDYAYFKQSPSVVMIDTTIIAEAPTRFLVSGMGDALSTYFEAR 178 DAP S +V+YT EG FD Y +P++V++DT I+A AP R L +G+GDAL+T+FEAR Sbjct: 134 DAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGAPARLLAAGIGDALATWFEAR 193 Query: 179 ATARSFSNVNAGLPCGVREGVSQEAKGTKAALALARVCYETLLEDGVKAKLACDQNVVTP 238 A +RS + AG C T+AALALA +CY TLLE+G KA LA +Q+VVTP Sbjct: 194 ACSRSGATTMAGGKC------------TQAALALAELCYNTLLEEGEKAMLAAEQHVVTP 241 Query: 239 ALENIIETNIXXXXXXXXXXXXXXXHAIHNGLTVLEGSHHYYHGEKVAFSTLAQLVLENA 298 ALE +IE N HA+HNGLT + +HHYYHGEKVAF TL QLVLEN+ Sbjct: 242 ALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENS 301 Query: 299 DSREIQEVLAFCASVGLPVCLADIGVHSITREELLEVARKACIPEESIHSMPFPVTPEAV 358 EI+ V A +VGLP+ LA + + ++ VA AC E+IH+MP TP+ V Sbjct: 302 PVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGETIHNMPGGATPDQV 361 Query: 359 AAAITVADQLGHAF 372 AA+ VADQ G F Sbjct: 362 YAALLVADQYGQRF 375 >ECs4874 [C] COG0371 Glycerol dehydrogenase and related enzymes Length = 380 Score = 305 bits (781), Expect = 9e-83, Method: Compositional matrix adjust. Identities = 164/374 (43%), Positives = 225/374 (60%), Gaps = 14/374 (3%) Query: 1 MRKVFISPTKYIQGEDELLNLGYFVKTFGDSALLIAHPDDVQRVKAKLDATSSKFGITLI 60 M ++ SP KYIQG D + LG ++K + L++ + ++ ++ + G+ + Sbjct: 14 MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFAQSTVEKSFKDAGLVVE 73 Query: 61 TGNFQGECSRQEVARLQALAREHACACTIGLGGGKAIDTAKCVAE--GDALIIVPTIAAT 118 F GECS+ E+ RL+ +A C +G+GGGK +DTAK +A G + I PTIA+T Sbjct: 74 IAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKALAHFMGVPVAIAPTIAST 133 Query: 119 DAPTSHSAVLYTPEGAFDDYAYFKQSPSVVMIDTTIIAEAPTRFLVSGMGDALSTYFEAR 178 DAP S +V+YT EG FD Y +P++V++DT I+A AP R L +G+GDAL+T+FEAR Sbjct: 134 DAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGAPARLLAAGIGDALATWFEAR 193 Query: 179 ATARSFSNVNAGLPCGVREGVSQEAKGTKAALALARVCYETLLEDGVKAKLACDQNVVTP 238 A +RS + AG C T+AALALA +CY TLLE+G KA LA +Q+VVTP Sbjct: 194 ACSRSGATTMAGGKC------------TQAALALAELCYNTLLEEGEKAMLAAEQHVVTP 241 Query: 239 ALENIIETNIXXXXXXXXXXXXXXXHAIHNGLTVLEGSHHYYHGEKVAFSTLAQLVLENA 298 ALE +IE N HA+HNGLT + +HHYYHGEKVAF TL QLVLEN+ Sbjct: 242 ALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVAFGTLTQLVLENS 301 Query: 299 DSREIQEVLAFCASVGLPVCLADIGVHSITREELLEVARKACIPEESIHSMPFPVTPEAV 358 EI+ V A +VGLP+ LA + + ++ VA AC E+IH+MP TP+ V Sbjct: 302 PVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGETIHNMPGGATPDQV 361 Query: 359 AAAITVADQLGHAF 372 AA+ VADQ G F Sbjct: 362 YAALLVADQYGQRF 375 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 25,676,749 Number of extensions: 1039450 Number of successful extensions: 2342 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 2300 Number of HSP's successfully gapped: 14 Length of query: 377 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 269 Effective length of database: 25,922,359 Effective search space: 6973114571 Effective search space used: 6973114571 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)