BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0538.0019.p (307 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_alsR [K] COG0583 Transcriptional regulator 117 2e-26 hcaR [K] COG0583 Transcriptional regulator 112 8e-25 lin2335 [K] COG0583 Transcriptional regulator 102 6e-22 BS_gltC [K] COG0583 Transcriptional regulator 95 1e-19 lin0491 [K] COG0583 Transcriptional regulator 95 2e-19 >BS_alsR [K] COG0583 Transcriptional regulator Length = 302 Score = 117 bits (294), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 69/214 (32%), Positives = 115/214 (53%), Gaps = 25/214 (11%) Query: 15 MNIQQLKCFVSLAETLNFSKTAEKLNLTQPAVSHNIKSLENELGILLFVRNKRTVNLTLA 74 M ++ L+ F+++AE L+F K A +LN+TQP +S IK LE E+G+ L R KR V LT A Sbjct: 1 MELRHLQYFIAVAEELHFGKAARRLNMTQPPLSQQIKQLEEEVGVTLLKRTKRFVELTAA 60 Query: 75 GKSFYEDMEGLLFRLDQSIKKAKRLSEKYESTLVIGYTETVFEKKVLPDMIRRFKEKYPQ 134 G+ F L ++ Q I+ A+R + + LVIG+ + + LP ++R +++K+P Sbjct: 61 GEIFLNHCRMALMQIGQGIELAQRTARGEQGLLVIGFVGSA-TYEFLPPIVREYRKKFPS 119 Query: 135 IQIQLKKSNLTREKEDLLNQKFDIIFTSEDNLGKDIDFCFYPVLQGSYV---------CV 185 ++I+L++ + +R++E+LL DI +P LQ + + CV Sbjct: 120 VKIELREISSSRQQEELLKGNIDI-------------GILHPPLQHTALHIETAQSSPCV 166 Query: 186 --LPKNHPFANESELDIEQLNHQSIILFDEHQSP 217 LPK HP ++ + IE L + II + P Sbjct: 167 LALPKQHPLTSKESITIEDLRDEPIITVAKEAWP 200 >hcaR [K] COG0583 Transcriptional regulator Length = 296 Score = 112 bits (279), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 72/251 (28%), Positives = 134/251 (53%), Gaps = 9/251 (3%) Query: 15 MNIQQLKCFVSLAETLNFSKTAEKLNLTQPAVSHNIKSLENELGILLFVRNKRTVNLTLA 74 M ++ L+ FV++A+ LNF++ AEKL+ +QP++S I+ LEN +G+ L VR+KR V LT A Sbjct: 1 MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVGVPLLVRDKRKVALTAA 60 Query: 75 GKSFYEDMEGLLFRLDQSIKKAKRLSEKYESTLVIGYTETVFEKKVLPDMIRRFKEKYPQ 134 G+ F +D +L + + + +A+++ ++ + L IG+ + E +LP ++ F+ + P Sbjct: 61 GECFLQDALAILEQAENAKLRARKIVQE-DRQLTIGFVPSA-EVNLLPKVLPMFRLRQPD 118 Query: 135 IQIQLKKSNLTREKEDLLNQKFDIIFTSEDNLGKDIDFCFYPVLQGSYVCVLPKNHPFAN 194 I+L T+++E + + D+ +ID+ + V VLP +HP A+ Sbjct: 119 TLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYL--ELFDEPLVVVLPVDHPLAH 176 Query: 195 ESELDIEQLNHQSIILFDEHQS---PPTLKKMNRKIIENCPDSIYTYGDTINTVHTMIKS 251 E E+ QL+ + + D S P +K + EN +I I ++ Sbjct: 177 EKEITAAQLDGVNFVSTDPVYSGSLAPIVKAWFAQ--ENSQPNIVQVATNILVTMNLVGM 234 Query: 252 DLGVSVLPDFV 262 LGV+++P ++ Sbjct: 235 GLGVTLIPGYM 245 >lin2335 [K] COG0583 Transcriptional regulator Length = 292 Score = 102 bits (255), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 77/280 (27%), Positives = 149/280 (53%), Gaps = 10/280 (3%) Query: 15 MNIQQLKCFVSLAETLNFSKTAEKLNLTQPAVSHNIKSLENELGILLFVRNKRTVNLTLA 74 MN+ L+ FV+LA +++K AEKL +TQP++SH I SLE ELGI LF + R + L+ A Sbjct: 1 MNLHHLRYFVTLAHMEHYTKAAEKLLITQPSLSHAISSLEQELGIPLFEKEGRNIGLSKA 60 Query: 75 GKSFYEDMEGLLFRLDQSIKKAKRLSEKYESTLVIGYTETVFEKKVLPDMIRRFKEKYPQ 134 GK F + +E L +D + K+ + E + + + +T+ ++P +++ F + P+ Sbjct: 61 GKVFLDYVEESLDMIDAGVATTKKAANG-EGQIDLAFLQTL-GTSLVPKLVQEFIQTEPE 118 Query: 135 IQIQLK-KSNLTRE-KEDLLNQKFDIIFTSEDNLGKDIDFCFYPVLQGSYVCVLPKNHPF 192 +I + ++ + + L +K+DI S+ L + + F P+ + V ++PK+HP Sbjct: 119 KKIDFVFHTGVSIDIIQGLKEKKYDIGICSK--LENERNIHFTPIAKQELVLIVPKSHPL 176 Query: 193 ANESELD-IEQLNHQSIILFDEHQSPPTLKKMNRKIIENCPDSIYTYGDTINTVHTMIKS 251 A + +D IE + + I + P + + RKI E+ + D + + M+ Sbjct: 177 AEKDWIDLIETVPYPHIAFSKKSGLRPIIDDLFRKIGEDYKIAYEIEVDQV--IAGMVAQ 234 Query: 252 DLGVSVLPDFVIGEDSEIASVPL-SYTEEVVYGLACLRSE 290 + G++V+P+ I ++ +P+ S E + LA ++++ Sbjct: 235 NFGIAVVPNMPILNYVDVKVLPIRSPNWERFFYLAVVKNQ 274 >BS_gltC [K] COG0583 Transcriptional regulator Length = 300 Score = 95.1 bits (235), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 68/263 (25%), Positives = 133/263 (50%), Gaps = 23/263 (8%) Query: 15 MNIQQLKCFVSLAETLNFSKTAEKLNLTQPAVSHNIKSLENELGILLFVRNKRTVNLTLA 74 M+++QL+ F+ +AE + S+ A+ L++ Q A+S I +LE EL + LF R R + LT Sbjct: 1 MDVRQLRYFMEVAEREHVSEAADHLHVAQSAISRQIANLEEELNVTLFEREGRNIKLTPI 60 Query: 75 GKSFYEDMEGLLFRLDQSIKKAKRLSEKYESTLVIGYTETVFEKKVLPDMIRRFKEKYPQ 134 GK F ++ + +D + ++ + + T+ IG+ T ++LP +I FKE+YP Sbjct: 61 GKEFLIHVKTAMKAIDYAKEQIDEYLDPHRGTVKIGFP-TSLASQLLPTVISAFKEEYPH 119 Query: 135 IQIQLKKSNLTREKEDLLNQKFDI-----IFTSEDNLGKDIDFCFYPVLQGSYVCVLPKN 189 ++ L++ + E + N+ D+ + T+ ++ I F + Y V P N Sbjct: 120 VEFLLRQGSYKFLIEAVRNRDIDLALLGPVPTNFSDITGKILFT-----EKIYALV-PLN 173 Query: 190 HPFANESELDIEQLNHQSIILFDEHQSPPTLKKMNRKIIENCPDS-----IYTYGDTINT 244 HP A + + + L + +LF E L++M I+ C + + T G+ ++ Sbjct: 174 HPLAKQKTVHLIDLRNDQFVLFPEGF---VLREMA---IDTCKQAGFAPLVSTEGEDLDA 227 Query: 245 VHTMIKSDLGVSVLPDFVIGEDS 267 + ++ + +GV++LP+ E + Sbjct: 228 IKGLVSAGMGVTLLPESTFAETT 250 >lin0491 [K] COG0583 Transcriptional regulator Length = 297 Score = 94.7 bits (234), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 70/248 (28%), Positives = 120/248 (48%), Gaps = 8/248 (3%) Query: 15 MNIQQLKCFVSLAETLNFSKTAEKLNLTQPAVSHNIKSLENELGILLFVRNKRTVNLTLA 74 MN++QL F LAE +++ A +L++TQP++SH+I LE ELG+ LF + R + LT Sbjct: 1 MNLRQLYYFKKLAEKEHYTIAAAELSITQPSLSHSIAELERELGVYLFEKQGRNIRLTKY 60 Query: 75 GKSFYEDMEGLLFRLDQSIKKAKRLSEKYESTLVIGYTETVFEKKVLPDMIRRF--KEKY 132 G+ + +E L L++ K L+ + +G+ T+ V P++++ F E + Sbjct: 61 GRFYLTYVEKSLAELEKGEKLLHELTSPSHGNIDLGFIYTMGAHTV-PELVQNFTKAENH 119 Query: 133 PQIQIQLKKSNLTREKEDLLNQKFDIIFTSEDNLGKDIDFCFYPVLQGSYVCVLPKNHPF 192 I + DL N+KFD+ S DI+F P+ + V V+ +NHP Sbjct: 120 KDITFSFYQGATKSIIPDLKNEKFDLAICSYVENEPDIEFL--PLTKQELVIVVAENHPL 177 Query: 193 ANESELDIEQLNHQSIILF-DEHQSPPTLKKMNRKIIENCPDSIYTYGDTINTVHTMIKS 251 A +D++ S I F D P + + +I N I Y + + ++ Sbjct: 178 AKYDSIDLKDTADYSYIFFSDTSGLRPLIDSLFAEI--NIQPKIGCYVEEDTAMVGLVSV 235 Query: 252 DLGVSVLP 259 D G+S++P Sbjct: 236 DYGISIMP 243 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,861,908 Number of extensions: 836144 Number of successful extensions: 4694 Number of sequences better than 1.0e-05: 1513 Number of HSP's gapped: 3250 Number of HSP's successfully gapped: 1513 Length of query: 307 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 201 Effective length of database: 26,153,027 Effective search space: 5256758427 Effective search space used: 5256758427 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)