BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0538.0009.p (612 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA1068 [O] COG0326 Molecular chaperone, HSP90 family 155 2e-37 PA1596 [O] COG0326 Molecular chaperone, HSP90 family 106 1e-22 YMR186w [O] COG0326 Molecular chaperone, HSP90 family 105 2e-22 YPL240c [O] COG0326 Molecular chaperone, HSP90 family 93 2e-18 >PA1068 [O] COG0326 Molecular chaperone, HSP90 family Length = 636 Score = 155 bits (391), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 157/561 (27%), Positives = 258/561 (45%), Gaps = 36/561 (6%) Query: 10 RFQVNLSGMINILSNHLYSSPKVFLRELLQNGIDAITARQAYSPDGYEGKIHVEVSGTST 69 R V+L+G++++LS HLYS+P V LREL+QN D+I R+ PD ++G +EV G S Sbjct: 38 RAGVDLNGVMSVLSKHLYSTPTVALRELVQNAHDSILRRRLEQPD-WQGPSRIEVIGDSA 96 Query: 70 GATLLVEDNGIGLNEAEIHEFLAMIGQSSKRG-EDFLSTNTSFIGRFGIGLLSCFMVSDD 128 T+ + D G GL E EIH +LA +G RG ++ IG FG+G LS F+++ Sbjct: 97 SNTVRIVDTGAGLTEHEIHAYLATVGVGYTRGLRQSGHEDSGLIGMFGLGFLSAFVLARR 156 Query: 129 IVMVTQSAKGGPALE--WRGKPDGTYTIRKLEGQHAPGTKVFLRCKEGSEAYFEENNLQE 186 + + T S + P L + Y++ + + GT++ L ++ A + L+E Sbjct: 157 VSVRTTSYQ-QPELGFCYVSSNAEQYSVTPMPARPV-GTEITLELQDEYSALTQAARLRE 214 Query: 187 GLFHYGALLPYPIQLVSDRNTRLINPLTPPW--VKDPQ---LARKHRDEVLAFGKQVLGE 241 L Y LL PI + D + INP PPW D L R+ RD L F + Sbjct: 215 ILERYCVLLREPIHVGGDAQS--INPEPPPWRLAADAALHPLQRQRRD--LEFAARF--- 267 Query: 242 TFRDFIPLHTASGR------TGGIAFILPQAV-NLNAKRNHRVYLKHMLVSEAASNILPD 294 DF P+ R G+ +I + RN V+L+ ML+ + A ++LP Sbjct: 268 -EHDFEPICCLPVRPDERVDVQGLLWIQDGGTYGTSDNRNLSVFLRGMLLDDDARDLLPS 326 Query: 295 WAFFVKCLIWTDELQPTASREHFYENAQLEQVREELGNSIRQELMRMAEYDPDRLQSIIS 354 WA FV +I ++ L PTASRE + ++ L ++ L +A P+ + ++ Sbjct: 327 WAGFVGGVIESNRLTPTASREDLQRDDHYAAIQHALAEALIAGLGEVARQQPEAWRRVLL 386 Query: 355 LHALSMKALAVEDLSFYSIIHEWLPFESTFGRKPLGELKQHPPLYFTATLDEYRQITHVA 414 H ++ A+ D ++++ + L ++ G P EL ++ D + + Sbjct: 387 RHNEALLGAALCDERLFALMMDSLRVPTSQGDLPASELLSRGAVHVLLDNDSGFE-EMLF 445 Query: 415 SAQSMLVVNGGFIYDAELLSRLPLVDPDVETERLLPEEVSLSFTDITPQERLDYYESVRL 474 A + V +G L R + RL+ + +RL +E+ L Sbjct: 446 RAMGVPVAHGNRYAVVPFLRRW----AQAKGMRLVELGTEQGNRQLFRLDRLPEHETGWL 501 Query: 475 ADSVLQKFRCQVQLRRFKPEEIPVLYTLSEESAQWRVL---EATKEVSTDALSSVLGSLG 531 A+ L Q+ + RF PEE+P++ ++ R L EA K +ST AL + Sbjct: 502 AEH-LGGAEEQLVIARFSPEELPLVVVPDRDAELKRRLEDDEADKRISTAAL-RLARQFT 559 Query: 532 ATLKDAAYATLYFNLNNPVIE 552 L + + LY NL+NP ++ Sbjct: 560 RKLDNRQPSRLYVNLDNPAVQ 580 >PA1596 [O] COG0326 Molecular chaperone, HSP90 family Length = 634 Score = 106 bits (265), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 99/413 (23%), Positives = 191/413 (46%), Gaps = 41/413 (9%) Query: 5 NRDTYRFQVNLSGMINILSNHLYSSPKVFLRELLQNGIDA---ITARQAYSPDGYEG--K 59 ++T FQ + +++++ + LYS+ ++FLREL+ N DA + +P+ EG + Sbjct: 6 QKETLGFQTEVKQLLHLMIHSLYSNKEIFLRELISNASDAADKLRFEALANPELLEGGAE 65 Query: 60 IHVEVSGTSTGATLLVEDNGIGLNEAEIHEFLAMIGQSSKRGEDFLST-------NTSFI 112 + + VS T+ +EDNGIG++ ++ L I +S DFL ++ I Sbjct: 66 LKIRVSFDKEANTVTLEDNGIGMSREDVVTHLGTIAKSGT--ADFLKNLSGDQKKDSHLI 123 Query: 113 GRFGIGLLSCFMVSDDIVMVTQSAKGGPALE---WRGKPDGTYTIRKLEGQHAPGTKVFL 169 G+FG+G S F+V+D + + ++ A G PA E W K +G + + ++ + GT++ L Sbjct: 124 GQFGVGFYSAFIVADKVDVYSRRA-GQPASEGVHWSSKGEGEFDVATID-KPERGTRIVL 181 Query: 170 RCKEGSEAYFEENNLQEGLFHYGALLPYPIQLVSD-RNTRLINPLTPPWV---KDPQLAR 225 K+G E + + L+ + Y + PI+L + P P W + L Sbjct: 182 HLKKGEEEFADGWRLRNVIKKYSDHIALPIELPKEFHGEEADKPAEPEWETVNRASALWT 241 Query: 226 KHRDEVL-----AFGKQVLGETFRDFIPLHTASGRTGG------IAFILPQA----VNLN 270 + R EV F K V F + PL + + G + ++ +A + Sbjct: 242 RPRAEVKDEEYQEFYKHV-AHDFEN--PLSWSHNKVEGKLEYTSLLYVPGRAPFDLYHRE 298 Query: 271 AKRNHRVYLKHMLVSEAASNILPDWAFFVKCLIWTDELQPTASREHFYENAQLEQVREEL 330 A R ++Y++ + + + A LP + F+K ++ +++L SRE ++ ++ ++ L Sbjct: 299 APRGLKLYVQRVFIMDQADEFLPLYLRFIKGVVDSNDLSLNVSREILQKDPVIDSMKSAL 358 Query: 331 GNSIRQELMRMAEYDPDRLQSIISLHALSMKALAVEDLSFYSIIHEWLPFEST 383 + L ++A+ +P++ ++ +K ED I L F ST Sbjct: 359 TKRVLDMLEKLAKNEPEQYKTFWKNFGQVLKEGPAEDFGNKEKIAGLLRFAST 411 >YMR186w [O] COG0326 Molecular chaperone, HSP90 family Length = 705 Score = 105 bits (262), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 106/497 (21%), Positives = 214/497 (43%), Gaps = 87/497 (17%) Query: 7 DTYRFQVNLSGMINILSNHLYSSPKVFLRELLQNGIDAITA--RQAYS-PDGYEGK--IH 61 +T+ FQ ++ +++++ N +YS+ ++FLREL+ N DA+ QA S P E + + Sbjct: 4 ETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYQALSDPKQLETEPDLF 63 Query: 62 VEVSGTSTGATLLVEDNGIGLNEAEIHEFLAMIGQSSKRGEDFLS-----TNTSFIGRFG 116 + ++ L + D+GIG+ +AE+ L I +S + F+ + S IG+FG Sbjct: 64 IRITPKPEEKVLEIRDSGIGMTKAELINNLGTIAKSGTKA--FMEALSAGADVSMIGQFG 121 Query: 117 IGLLSCFMVSDDIVMVTQSAKGGPALEWRGKPDGTYTIRKLEGQHAPGTKVFLR--CKEG 174 +G S F+V+D + +++++ + + W G++T+ E G LR K+ Sbjct: 122 VGFYSLFLVADRVQVISKNNEDEQYI-WESNAGGSFTVTLDEVNERIGRGTVLRLFLKDD 180 Query: 175 SEAYFEENNLQEGLFHYGALLPYPIQLVSDRNTRLINPL--------------------- 213 Y EE ++E + + + YPIQL+ + P+ Sbjct: 181 QLEYLEEKRIKEVIKRHSEFVAYPIQLLVTKEVEKEVPIPEEEKKDEEKKDEDDKKPKLE 240 Query: 214 -----------------------------TPPWVKDPQLARKHRDEVLAFGKQVLGETFR 244 P W ++P + ++E AF K + + + Sbjct: 241 EVDEEEEEKKPKTKKVKEEVQELEELNKTKPLWTRNP--SDITQEEYNAFYKSISND-WE 297 Query: 245 DFIPLHTASGRTGG------IAFILPQA----VNLNAKRNH-RVYLKHMLVSEAASNILP 293 D PL+ G I FI +A K+N+ ++Y++ + +++ A +++P Sbjct: 298 D--PLYVKHFSVEGQLEFRAILFIPKRAPFDLFESKKKKNNIKLYVRRVFITDEAEDLIP 355 Query: 294 DWAFFVKCLIWTDELQPTASREHFYENAQLEQVREELGNSIRQELMRMAEYDPDRLQSII 353 +W FVK ++ +++L SRE +N ++ +R+ + + + +AE D ++ Sbjct: 356 EWLSFVKGVVDSEDLPLNLSREMLQQNKIMKVIRKNIVKKLIEAFNEIAE-DSEQFDKFY 414 Query: 354 SLHALSMKALAVEDLSFYSIIHEWLPFESTFGRKPLGELKQHPPLYFTATLDEYRQITHV 413 S A ++K ED + + + L + ST L L + + E+++ + Sbjct: 415 SAFAKNIKLGVHEDTQNRAALAKLLRYNSTKSVDELTSLTD-----YVTRMPEHQKNIYY 469 Query: 414 ASAQSMLVVNGGFIYDA 430 + +S+ V DA Sbjct: 470 ITGESLKAVEKSPFLDA 486 >YPL240c [O] COG0326 Molecular chaperone, HSP90 family Length = 311 Score = 92.8 bits (229), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 9/208 (4%), Positives = 37/208 (17%), Gaps = 15/208 (7%) Query: 7 DTYRFQVNLSGMINILSNHLYSSPKVFLRELLQNGIDA---ITARQAYSPDGYEGK--IH 61 + ++ + + ++ L+ G+ + + + + + Sbjct: 4 NFFKHLLESLKNLKRNGWMTVAAVSSVTITLVLVGLFLSVILNTSKISNGIENNVRIVAY 63 Query: 62 VEVSGTSTGATLLVEDNGIGLNEAEIHEFLAMIGQSSKRGEDFLS-----TNTSFIGRFG 116 +++ + + H + + Sbjct: 64 MKLDVHDQETKIQDPKDNKKTINNPDHLKIYDSIKKVPNV--KTVEFSSKEAQLKELTKA 121 Query: 117 IGLLSCFMVSDDIVMVTQSAKGGPALEWRGKPDGTYTIRKLEGQHAPGTKVFLR--CKEG 174 +G D + K + + + A + Sbjct: 122 LGNNWNLFKGDANPLYDAYIVEADKP-ANVKSVTSKISKLSGIEKATYGGANTAKLFQIA 180 Query: 175 SEAYFEENNLQEGLFHYGALLPYPIQLV 202 L L + Sbjct: 181 QGIRTWGTAAAILLIFVAVFLISNTIRI 208 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 45,246,519 Number of extensions: 1858866 Number of successful extensions: 3226 Number of sequences better than 1.0e-05: 4 Number of HSP's gapped: 3214 Number of HSP's successfully gapped: 7 Length of query: 612 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 500 Effective length of database: 25,461,023 Effective search space: 12730511500 Effective search space used: 12730511500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 119 (50.4 bits)