BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0538.0005.p (299 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc3663 [K] COG2207 AraC-type DNA-binding domain-containing prot... 179 4e-45 AGc3663A [R] COG1064 Zn-dependent alcohol dehydrogenases 179 4e-45 AGl249 [R] COG3608 Predicted deacylase 174 1e-43 AGl2465 [R] COG0705 Uncharacterized membrane protein (homolog of... 174 1e-43 AGl2460 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase 174 1e-43 >AGc3663 [K] COG2207 AraC-type DNA-binding domain-containing proteins Length = 289 Score = 179 bits (455), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 0/297 (0%), Positives = 0/297 (0%), Gaps = 9/297 (3%) Query: 3 NVLDEIIDLMKGAGTR--QIETGVPGLSMIKGDIPAHQLAALYEPMIGFTVQGTKILSIG 60 + + + G + + +K A A+ F + Sbjct: 6 DSRETLTINAFVVGYPIKHSRSPIIHSYWLKKFGIAGSYTAVEVSPDDFPKFIATLKEGK 65 Query: 61 ERSTDLEGPSYYVLPVHVPATASVHPDRYGRPYMSLGLKLNQNVLQSLLRDLPENLLPTA 120 + + + + + Sbjct: 66 PGAAVGGNATIPHKEAAYRLADHPDALAEELGAANTIWMEEGKLHATNTDGYGFV--SNL 123 Query: 121 SGHFAACEMDI-----EFMEAWLRLLRLSKTSRDIPALAPAYEREILYRVLMGPQGWYLR 175 + A +++ + E + Sbjct: 124 DERHPGWDKTQRAVVFGAGGASRAVIQSLRDRDVAEIHVVNRTVERARELADRFGPRVFS 183 Query: 176 QFGLRESNLSKISEIVKWFRSNFMKPIDIGEMASKSGMAINTFHRQFKRATGLSPIQFQK 235 + + + S M + + A + + Sbjct: 184 HPQAALQEVMHGAGLFVNTTSLGMNGTEAPHLDFSHLAANAVVTDIVYVPLKTPILNMAQ 243 Query: 236 QLRLLEARNLIAFEGYPVASAAYHVGYQSPSQFNREYSRFFGSSPARDTENLRRIES 292 Sbjct: 244 300 >AGc3663A [R] COG1064 Zn-dependent alcohol dehydrogenases Length = 289 Score = 179 bits (455), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 0/297 (0%), Positives = 0/297 (0%), Gaps = 9/297 (3%) Query: 3 NVLDEIIDLMKGAGTR--QIETGVPGLSMIKGDIPAHQLAALYEPMIGFTVQGTKILSIG 60 + + + G + + +K A A+ F + Sbjct: 6 DSRETLTINAFVVGYPIKHSRSPIIHSYWLKKFGIAGSYTAVEVSPDDFPKFIATLKEGK 65 Query: 61 ERSTDLEGPSYYVLPVHVPATASVHPDRYGRPYMSLGLKLNQNVLQSLLRDLPENLLPTA 120 + + + + + Sbjct: 66 PGAAVGGNATIPHKEAAYRLADHPDALAEELGAANTIWMEEGKLHATNTDGYGFV--SNL 123 Query: 121 SGHFAACEMDI-----EFMEAWLRLLRLSKTSRDIPALAPAYEREILYRVLMGPQGWYLR 175 + A +++ + E + Sbjct: 124 DERHPGWDKTQRAVVFGAGGASRAVIQSLRDRDVAEIHVVNRTVERARELADRFGPRVFS 183 Query: 176 QFGLRESNLSKISEIVKWFRSNFMKPIDIGEMASKSGMAINTFHRQFKRATGLSPIQFQK 235 + + + S M + + A + + Sbjct: 184 HPQAALQEVMHGAGLFVNTTSLGMNGTEAPHLDFSHLAANAVVTDIVYVPLKTPILNMAQ 243 Query: 236 QLRLLEARNLIAFEGYPVASAAYHVGYQSPSQFNREYSRFFGSSPARDTENLRRIES 292 Sbjct: 244 300 >AGl249 [R] COG3608 Predicted deacylase Length = 320 Score = 174 bits (442), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 104/280 (37%), Positives = 150/280 (53%), Gaps = 2/280 (0%) Query: 21 ETGVPGLSMIKGDIPAHQLAALYEPMIGFTVQGTKILSIGERSTDLEGPSYYVLPVHVPA 80 ET +PGL +I+ + +YEP + QG K +G+ S + SY V V +P Sbjct: 37 ETQLPGLKLIRCSETTLPMPVVYEPTLCLVAQGRKQAVLGQTSFVYDPSSYLVASVDLPV 96 Query: 81 TASVHPDRYGRPYMSLGLKLNQNVLQSLLRDLPENLLPTASGHFAAC--EMDIEFMEAWL 138 SV +PY+S+ L L+ L L P +G ++D ++A Sbjct: 97 MGSVIEASKDKPYLSMQLDLDTVELADLAIRYPSEQHAPINGPIGLLLNKVDEGIIDAAS 156 Query: 139 RLLRLSKTSRDIPALAPAYEREILYRVLMGPQGWYLRQFGLRESNLSKISEIVKWFRSNF 198 RLL L T RDI AL+P +REILYR++ G G LRQ +++++I+ + W R +F Sbjct: 157 RLLALLDTPRDIDALSPLAKREILYRLITGVGGAALRQIVQAGAHVNQIARSIVWIRKHF 216 Query: 199 MKPIDIGEMASKSGMAINTFHRQFKRATGLSPIQFQKQLRLLEARNLIAFEGYPVASAAY 258 + + A +GM+ +TFH FK T +SP++F+ QLRL EAR L+ EG A+A + Sbjct: 217 RETCSVEAGAEVAGMSRSTFHAHFKAVTNMSPLEFRTQLRLQEARRLMLSEGLDAATAGF 276 Query: 259 HVGYQSPSQFNREYSRFFGSSPARDTENLRRIESTRISPL 298 VGY SPSQF+REY R FG SPA+ LR+ I PL Sbjct: 277 QVGYASPSQFSREYGRVFGMSPAKHANALRQSPKQWIEPL 316 >AGl2465 [R] COG0705 Uncharacterized membrane protein (homolog of Drosophila rhomboid) Length = 320 Score = 174 bits (442), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 104/280 (37%), Positives = 150/280 (53%), Gaps = 2/280 (0%) Query: 21 ETGVPGLSMIKGDIPAHQLAALYEPMIGFTVQGTKILSIGERSTDLEGPSYYVLPVHVPA 80 ET +PGL +I+ + +YEP + QG K +G+ S + SY V V +P Sbjct: 37 ETQLPGLKLIRCSETTLPMPVVYEPTLCLVAQGRKQAVLGQTSFVYDPSSYLVASVDLPV 96 Query: 81 TASVHPDRYGRPYMSLGLKLNQNVLQSLLRDLPENLLPTASGHFAAC--EMDIEFMEAWL 138 SV +PY+S+ L L+ L L P +G ++D ++A Sbjct: 97 MGSVIEASKDKPYLSMQLDLDTVELADLAIRYPSEQHAPINGPIGLLLNKVDEGIIDAAS 156 Query: 139 RLLRLSKTSRDIPALAPAYEREILYRVLMGPQGWYLRQFGLRESNLSKISEIVKWFRSNF 198 RLL L T RDI AL+P +REILYR++ G G LRQ +++++I+ + W R +F Sbjct: 157 RLLALLDTPRDIDALSPLAKREILYRLITGVGGAALRQIVQAGAHVNQIARSIVWIRKHF 216 Query: 199 MKPIDIGEMASKSGMAINTFHRQFKRATGLSPIQFQKQLRLLEARNLIAFEGYPVASAAY 258 + + A +GM+ +TFH FK T +SP++F+ QLRL EAR L+ EG A+A + Sbjct: 217 RETCSVEAGAEVAGMSRSTFHAHFKAVTNMSPLEFRTQLRLQEARRLMLSEGLDAATAGF 276 Query: 259 HVGYQSPSQFNREYSRFFGSSPARDTENLRRIESTRISPL 298 VGY SPSQF+REY R FG SPA+ LR+ I PL Sbjct: 277 QVGYASPSQFSREYGRVFGMSPAKHANALRQSPKQWIEPL 316 >AGl2460 [I] COG1250 3-hydroxyacyl-CoA dehydrogenase Length = 320 Score = 174 bits (442), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 104/280 (37%), Positives = 150/280 (53%), Gaps = 2/280 (0%) Query: 21 ETGVPGLSMIKGDIPAHQLAALYEPMIGFTVQGTKILSIGERSTDLEGPSYYVLPVHVPA 80 ET +PGL +I+ + +YEP + QG K +G+ S + SY V V +P Sbjct: 37 ETQLPGLKLIRCSETTLPMPVVYEPTLCLVAQGRKQAVLGQTSFVYDPSSYLVASVDLPV 96 Query: 81 TASVHPDRYGRPYMSLGLKLNQNVLQSLLRDLPENLLPTASGHFAAC--EMDIEFMEAWL 138 SV +PY+S+ L L+ L L P +G ++D ++A Sbjct: 97 MGSVIEASKDKPYLSMQLDLDTVELADLAIRYPSEQHAPINGPIGLLLNKVDEGIIDAAS 156 Query: 139 RLLRLSKTSRDIPALAPAYEREILYRVLMGPQGWYLRQFGLRESNLSKISEIVKWFRSNF 198 RLL L T RDI AL+P +REILYR++ G G LRQ +++++I+ + W R +F Sbjct: 157 RLLALLDTPRDIDALSPLAKREILYRLITGVGGAALRQIVQAGAHVNQIARSIVWIRKHF 216 Query: 199 MKPIDIGEMASKSGMAINTFHRQFKRATGLSPIQFQKQLRLLEARNLIAFEGYPVASAAY 258 + + A +GM+ +TFH FK T +SP++F+ QLRL EAR L+ EG A+A + Sbjct: 217 RETCSVEAGAEVAGMSRSTFHAHFKAVTNMSPLEFRTQLRLQEARRLMLSEGLDAATAGF 276 Query: 259 HVGYQSPSQFNREYSRFFGSSPARDTENLRRIESTRISPL 298 VGY SPSQF+REY R FG SPA+ LR+ I PL Sbjct: 277 QVGYASPSQFSREYGRVFGMSPAKHANALRQSPKQWIEPL 316 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,744,105 Number of extensions: 903546 Number of successful extensions: 3258 Number of sequences better than 1.0e-05: 59 Number of HSP's gapped: 3244 Number of HSP's successfully gapped: 59 Length of query: 299 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 193 Effective length of database: 26,153,027 Effective search space: 5047534211 Effective search space used: 5047534211 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)