BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0538.0004.p (267 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SA1074 [IQR] COG1028 Dehydrogenases with different specificities... 134 1e-31 Ta0191 [IQR] COG1028 Dehydrogenases with different specificities... 127 1e-29 BS_fabG [IQR] COG1028 Dehydrogenases with different specificitie... 127 2e-29 AGl2880 [R] COG4618 ABC-type protease/lipase transport system, A... 123 2e-28 AGl2875 [P] COG1629 Outer membrane receptor proteins, mostly Fe ... 123 2e-28 >SA1074 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 246 Score = 134 bits (337), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 88/254 (34%), Positives = 134/254 (52%), Gaps = 12/254 (4%) Query: 16 LMKIAIVTGGSNGIGKATALELGKRGISVILTYNSYKDRAEGVVKEIEKNKGVRAFALKL 75 + K A+VTG S GIG++ AL+L + G +V + Y K++AE VV+EI K KGV +FA++ Sbjct: 3 MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVVEEI-KAKGVDSFAIQA 61 Query: 76 DLTRISTFEGFILEVKKSLQDIWNRTTFDYLVNNGGVGGPMMFTEMSEEYFDKILNTNFK 135 ++ + I EV + + D LVNN G+ + M E+ +D +++TN K Sbjct: 62 NVADADEVKAMIKEV------VSQFGSLDVLVNNAGITRDNLLMRMKEQEWDDVIDTNLK 115 Query: 136 GPAFLTQQLVGFM--EDAGAIVNTTSSSKNQSFPGYSIYGSLKAAFSTWTRYIAKELAPR 193 G Q+ M + +GAI+N +S PG + Y + KA T+ A+ELA R Sbjct: 116 GVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVIGLTKSAARELASR 175 Query: 194 QIRVNAVSPGPTHSNFGDGVFDKHPEFIKPLAEQSAFGRIGQPEDMAKVIVSLLSDDFGW 253 I VNAV+PG S+ D + D E + + Q R GQ D+A + L SD + Sbjct: 176 GITVNAVAPGFIVSDMTDALSD---ELKEQMLTQIPLARFGQDTDIANTVAFLASDKAKY 232 Query: 254 VTAQDIEVSGGHLL 267 +T Q I V+GG + Sbjct: 233 ITGQTIHVNGGMYM 246 >Ta0191 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 268 Score = 127 bits (320), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 85/253 (33%), Positives = 134/253 (52%), Gaps = 11/253 (4%) Query: 18 KIAIVTGGSNGIGKATALELGKRGISVILTYNSYKDRAEGVVKEIEKNKGVRAFALKLDL 77 KIA+VTG S+G+G A A + + G V + Y S KDRA+ +V EI KN G A A D+ Sbjct: 16 KIALVTGSSSGLGAAIAKYMARAGAKVAVHYRSGKDRADAIVDEI-KNDGGFAMAFYGDV 74 Query: 78 TRISTFEGFILEVKKSLQDIWNRTTFDYLVNNGGVGGPM-MFTEMSEEYFDKILNTNFKG 136 ++ + E+ L T D LVNN G+ G + E + ++K++ N G Sbjct: 75 SKKEDVQKLFSEIDSKL------GTVDILVNNAGIDGKRELVGEDDPDDWEKVIEVNLMG 128 Query: 137 PAFLTQQLVGFM--EDAGAIVNTTSSSKNQSFPGYSIYGSLKAAFSTWTRYIAKELAPRQ 194 P + ++ V M + +G I+N TS + + GY+ Y S KA S +T+ +A+EL+ Sbjct: 129 PYYCAREAVKRMKPKKSGVIINITSVHEYVPWSGYTAYSSAKAGLSMFTKALAQELSDYN 188 Query: 195 IRVNAVSPGPTHSNFGDGVFDKHPEFIKPLAEQSAFGRIGQPEDMAKVIVSLLSDDFGWV 254 IRV A++PG + V+ +PE +K L + A R+G+ +D+ + V L SD ++ Sbjct: 189 IRVVAIAPGAIKTPINKDVW-GNPESLKDLLNKIAMPRLGEVDDIGQAAVFLASDLASYI 247 Query: 255 TAQDIEVSGGHLL 267 T + V GG L Sbjct: 248 TGTTLLVDGGMAL 260 >BS_fabG [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 246 Score = 127 bits (318), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 84/253 (33%), Positives = 132/253 (52%), Gaps = 14/253 (5%) Query: 18 KIAIVTGGSNGIGKATALELGKRGISVILTYNSYKDRAEGVVKEIEKNKGVRAFALKLDL 77 K AIVTG S GIG++ AL+L K G +V++ Y+ + +A VV EI K+ G +A A+K D+ Sbjct: 5 KTAIVTGASRGIGRSIALDLAKSGANVVVNYSGNEAKANEVVDEI-KSMGRKAIAVKADV 63 Query: 78 TRISTFEGFILEVKKSLQDIWNRTTFDYLVNNGGVGGPMMFTEMSEEYFDKILNTNFKGP 137 + + I E +T D LVNN G+ + M E+ +D ++N N KG Sbjct: 64 SNPEDVQNMIKETLSVF------STIDILVNNAGITRDNLIMRMKEDEWDDVININLKGV 117 Query: 138 AFLTQQLVGFM--EDAGAIVNTTSSSKNQSFPGYSIYGSLKAAFSTWTRYIAKELAPRQI 195 T+ + M + +G I+N +S PG + Y + KA T+ AKELA R I Sbjct: 118 FNCTKAVTRQMMKQRSGRIINVSSIVGVSGNPGQANYVAAKAGVIGLTKSSAKELASRNI 177 Query: 196 RVNAVSPGPTHSNFGDGVF-DKHPEFIKPLAEQSAFGRIGQPEDMAKVIVSLLSDDFGWV 254 VNA++PG ++ D + D E +K Q R G+P D++ V+ L S+ ++ Sbjct: 178 TVNAIAPGFISTDMTDKLAKDVQDEMLK----QIPLARFGEPSDVSSVVTFLASEGARYM 233 Query: 255 TAQDIEVSGGHLL 267 T Q + + GG ++ Sbjct: 234 TGQTLHIDGGMVM 246 >AGl2880 [R] COG4618 ABC-type protease/lipase transport system, ATPase and permease components Length = 249 Score = 123 bits (309), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 86/251 (34%), Positives = 128/251 (50%), Gaps = 13/251 (5%) Query: 18 KIAIVTGGSNGIGKATALELGKRGISVILTYNSYKDRAEGVVKEIEKNKGVRAFALKLDL 77 KIA++TG S G+G A A G SV++ Y+S K AE VV+EI + G A A+K D+ Sbjct: 8 KIAVITGASKGLGAAIARSFAAEGASVVVNYSSSKSAAEAVVEEI-IDAGGTAIAVKADM 66 Query: 78 TRISTFEGFILEVKKSLQDIWNRTTFDYLVNNGGVGGPMMFTEMSEEYFDKILNTNFKGP 137 + + E E+ + + R D LVNN GV +S + F K + N G Sbjct: 67 SEPAEVE----ELFAATNAAFGRV--DILVNNAGVYDFQPLENLSIDLFRKHMELNVFGY 120 Query: 138 AFLTQQLVGFMEDAGAIVNTTSSSKNQSFPGYSIYGSLKAAFSTWTRYIAKELAPRQIRV 197 ++ V +M + G+IVN +S+ S+Y + K A TR ++ ELAPR+IRV Sbjct: 121 LLAIKEAVKYMAEGGSIVNMSSTVTIFGPENASVYTASKGAIDGLTRALSNELAPRKIRV 180 Query: 198 NAVSPGPTHSNFGDGVFDK---HPEFIKPLAEQSAFGRIGQPEDMAKVIVSLLSDDFGWV 254 NA+ PG + DGV H +F + Q+ R+G PED+ + L SD+ W Sbjct: 181 NAIKPGVVDT---DGVQAGGFLHFDFGPMIMAQTPLKRLGVPEDIIPAAIFLASDESSWT 237 Query: 255 TAQDIEVSGGH 265 T + ++GGH Sbjct: 238 TGEFFILAGGH 248 >AGl2875 [P] COG1629 Outer membrane receptor proteins, mostly Fe transport Length = 249 Score = 123 bits (309), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 86/251 (34%), Positives = 128/251 (50%), Gaps = 13/251 (5%) Query: 18 KIAIVTGGSNGIGKATALELGKRGISVILTYNSYKDRAEGVVKEIEKNKGVRAFALKLDL 77 KIA++TG S G+G A A G SV++ Y+S K AE VV+EI + G A A+K D+ Sbjct: 8 KIAVITGASKGLGAAIARSFAAEGASVVVNYSSSKSAAEAVVEEI-IDAGGTAIAVKADM 66 Query: 78 TRISTFEGFILEVKKSLQDIWNRTTFDYLVNNGGVGGPMMFTEMSEEYFDKILNTNFKGP 137 + + E E+ + + R D LVNN GV +S + F K + N G Sbjct: 67 SEPAEVE----ELFAATNAAFGRV--DILVNNAGVYDFQPLENLSIDLFRKHMELNVFGY 120 Query: 138 AFLTQQLVGFMEDAGAIVNTTSSSKNQSFPGYSIYGSLKAAFSTWTRYIAKELAPRQIRV 197 ++ V +M + G+IVN +S+ S+Y + K A TR ++ ELAPR+IRV Sbjct: 121 LLAIKEAVKYMAEGGSIVNMSSTVTIFGPENASVYTASKGAIDGLTRALSNELAPRKIRV 180 Query: 198 NAVSPGPTHSNFGDGVFDK---HPEFIKPLAEQSAFGRIGQPEDMAKVIVSLLSDDFGWV 254 NA+ PG + DGV H +F + Q+ R+G PED+ + L SD+ W Sbjct: 181 NAIKPGVVDT---DGVQAGGFLHFDFGPMIMAQTPLKRLGVPEDIIPAAIFLASDESSWT 237 Query: 255 TAQDIEVSGGH 265 T + ++GGH Sbjct: 238 TGEFFILAGGH 248 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.135 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,865,592 Number of extensions: 801831 Number of successful extensions: 2864 Number of sequences better than 1.0e-05: 317 Number of HSP's gapped: 2293 Number of HSP's successfully gapped: 317 Length of query: 267 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 162 Effective length of database: 26,268,361 Effective search space: 4255474482 Effective search space used: 4255474482 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 115 (48.9 bits)