BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0535.0017.p (351 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2727 [T] COG0642 Signal transduction histidine kinase 183 3e-46 BS_phoR_3 [T] COG0642 Signal transduction histidine kinase 139 6e-33 BS_resE_4 [T] COG0642 Signal transduction histidine kinase 127 2e-29 TM1654_2 [T] COG0642 Signal transduction histidine kinase 127 3e-29 ECs0608 [T] COG0642 Signal transduction histidine kinase 122 6e-28 >lin2727 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 183 bits (465), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 0/275 (0%), Positives = 0/275 (0%), Gaps = 2/275 (0%) Query: 76 ASRSMVKTIREFIEATQRLAAGDFSTRLYLKSPPEYRILSENFNRMAEELGSIEILRTDF 135 Sbjct: 183 242 Query: 136 VNNFSHEFKTPIVSIKGFAEILKYDDLSEEERDEYLDIVIEESARLASLASNVLELSKIE 195 Sbjct: 243 302 Query: 196 TQTILTDKQEVNVGEQIRQCVXXXXXXXXXXXXXXNLGLHDYSLSGNKEMLNQVWLNLLE 255 Sbjct: 303 362 Query: 256 NAVKFTPEQGTVTIEMKRTANSLEMVFSNTGSVIDPETLPRIFDKFYQADTSHATV--GN 313 Sbjct: 363 422 Query: 314 GLGLTIVQRIVNLHGGTVACESGPLQGTVFIVTLP 348 Sbjct: 423 457 >BS_phoR_3 [T] COG0642 Signal transduction histidine kinase Length = 431 Score = 139 bits (351), Expect = 6e-33, Method: Compositional matrix adjust. Identities = 16/241 (6%), Positives = 43/241 (17%), Gaps = 10/241 (4%) Query: 118 FNRMAEELGSIEILRTDFVNNFSHEFKTPIVSIKGFAEILKYDDLSEEER-DEYLDIVIE 176 + L + TP + +L + ++ Sbjct: 38 SVTGLL-IWHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNL 96 Query: 177 ESARLASLASNVLELSKIETQ----TILTDKQEVNVGEQIRQCVXXXXXXXXXXXXXXNL 232 + S + + Q++ Sbjct: 97 IKRFRSGAESLPDAVVLTTEEGGIFWCNGLAQQILGLRW--PEDNGQNILNLLRYPEFTQ 154 Query: 233 GLHDYSLSGNKEMLNQVWLNLLENAVKFTPEQGTVTIEMKRTANSLEMVFSNTGSVIDPE 292 L S ++ +L + +T +Q + + LE N + + E Sbjct: 155 YLKTRDFSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHE 214 Query: 293 TLPRIFDKFYQADT--SHATVGNGLGLTIVQRIVNLHGGTVACESGPLQGTVFIVTLPFA 350 + + G + + + Sbjct: 215 LRTPLTVLQGYLEMMNEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLL 274 Query: 351 A 351 Sbjct: 275 N 275 >BS_resE_4 [T] COG0642 Signal transduction histidine kinase Length = 269 Score = 127 bits (320), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 76/231 (32%), Positives = 132/231 (57%), Gaps = 15/231 (6%) Query: 128 IEILRTDFVNNFSHEFKTPIVSIKGFAEILKYDDL--SEEERDEYLDIVIEESARLASLA 185 ++ LR DF+ N SHE +TPI ++G++E + DD+ SEE+R E I+ +ES R+ L Sbjct: 41 LDKLREDFIANVSHELRTPISMLQGYSEAI-VDDIASSEEDRKEIAQIIYDESLRMGRLV 99 Query: 186 SNVLELSKIETQTILTDKQEVNVGEQIRQCVXXXXXXXXXXXXXXNLGL-HDYSLSG--- 241 +++L+L+++E+ +++NV E + + + N+ L HD SL+ Sbjct: 100 NDLLDLARMESGHTGLHYEKINVNEFLEKIIRKFSGVAKEK----NIALDHDISLTEEEF 155 Query: 242 --NKEMLNQVWLNLLENAVKFTPEQGTVTIEMKRTANSLEMVFSNTGSVIDPETLPRIFD 299 +++ + QV+ NL++NA++ T G+V+I + + L++ ++GS I E LP IF+ Sbjct: 156 MFDEDKMEQVFTNLIDNALRHTSAGGSVSISVHSVKDGLKIDIKDSGSGIPEEDLPFIFE 215 Query: 300 KFYQADTS--HATVGNGLGLTIVQRIVNLHGGTVACESGPLQGTVFIVTLP 348 +FY+AD + G GLGL IV+ IV H G++ S +GT F +P Sbjct: 216 RFYKADKARTRGRAGTGLGLAIVKNIVEAHNGSITVHSRIDKGTTFSFYIP 266 >TM1654_2 [T] COG0642 Signal transduction histidine kinase Length = 272 Score = 127 bits (319), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 85/225 (37%), Positives = 122/225 (54%), Gaps = 7/225 (3%) Query: 131 LRTDFVNNFSHEFKTPIVSIKGFAEILKYDDLSEEER-DEYLDIVIEESARLASLASNVL 189 +R +F+ SHE +TP+ SI G+AE L DDL +E +L I+ EESAR+ L +++L Sbjct: 45 MRREFIATVSHELRTPLTSIHGYAETLLEDDLENKELVKRFLKIIEEESARMTRLINDLL 104 Query: 190 ELSKIETQTILTDKQEVNVGEQIRQCVXXXXXXXXXXXXXXNLGLHDYSLSGNKEMLNQV 249 +L KIE + ++V++ E I + D + GNKE L Q+ Sbjct: 105 DLEKIEESEANFEMKDVDLCEVIEYVYRIIQPIAEENEVDLIVECEDVVVRGNKERLIQM 164 Query: 250 WLNLLENAVKFT--PEQG--TVTIEMKRTANSLEMVFSNTGSVIDPETLPRIFDKFYQAD 305 LNL++NAVK+T E+G V + T + + + +TG I E RIF+KFY+ D Sbjct: 165 LLNLVDNAVKYTSLKEKGEKKVWVRAYDTPDWVVVEVEDTGPGIPKEAQSRIFEKFYRVD 224 Query: 306 T--SHATVGNGLGLTIVQRIVNLHGGTVACESGPLQGTVFIVTLP 348 S G GLGLTIV+ IV+ HGG + ES QGT+ V LP Sbjct: 225 KARSRKMGGTGLGLTIVKTIVDKHGGKIEVESEINQGTLMRVLLP 269 >ECs0608 [T] COG0642 Signal transduction histidine kinase Length = 482 Score = 122 bits (307), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 76/268 (28%), Positives = 139/268 (51%), Gaps = 4/268 (1%) Query: 84 IREFIEATQRLAAGDFSTRLYLKSPP-EYRILSENFNRMAEELGSIEILRTDFVNNFSHE 142 IR Q + + D RL ++ P E L +FN M E + + +++F + +HE Sbjct: 213 IRSVSRQIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHE 272 Query: 143 FKTPIVSIKGFAEILKYDDLSEEERDEYLDIVIEESARLASLASNVLELSKIETQTILTD 202 +TPI ++ EI S++E ++ L +EE R+A + S++L L++ + ++ + Sbjct: 273 IRTPITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPE 332 Query: 203 KQEVNVGEQIRQCVXXXXXXXXXXXXXXNLGLHDYSLSGNKEMLNQVWLNLLENAVKFTP 262 K+ +N+ +++ + + ++G+ ML + NLL NA+++TP Sbjct: 333 KKMLNLADEVGKVFDFFEALAEDRGVELQFVGDECQVAGDPLMLRRALSNLLSNALRYTP 392 Query: 263 EQGTVTIEMKRTANSLEMVFSNTGSVIDPETLPRIFDKFYQADTSHATV--GNGLGLTIV 320 + + + + ++++ N G+ I PE LPR+FD+FY+ D S G+G+GL IV Sbjct: 393 PGEAIVVRCQTVDHLVQVIVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIV 452 Query: 321 QRIVNLHGGTVACESGPLQGTVFIVTLP 348 + IV H GTVA S +GT F++ LP Sbjct: 453 KSIVVAHKGTVAVTSNA-RGTRFVIVLP 479 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,472,383 Number of extensions: 732882 Number of successful extensions: 2376 Number of sequences better than 1.0e-05: 220 Number of HSP's gapped: 2027 Number of HSP's successfully gapped: 225 Length of query: 351 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 244 Effective length of database: 26,037,693 Effective search space: 6353197092 Effective search space used: 6353197092 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)