BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0535.0011.p (438 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM1033_1 [OU] COG0740 Protease subunit of ATP-dependent Clp pro... 91 3e-18 PA3551_1 [M] COG0836 Mannose-1-phosphate guanylyltransferase 78 4e-14 STM2084_1 [M] COG0836 Mannose-1-phosphate guanylyltransferase 77 7e-14 mlr5802_1 [M] COG0836 Mannose-1-phosphate guanylyltransferase 72 2e-12 mlr7607_1 [M] COG0836 Mannose-1-phosphate guanylyltransferase 67 4e-11 >STM1033_1 [OU] COG0740 Protease subunit of ATP-dependent Clp proteases Length = 200 Score = 90.9 bits (224), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 0/352 (0%), Positives = 0/352 (0%), Gaps = 47/352 (13%) Query: 1 VHTILLCGGSGQKXXXXXXXRS-KMFLELLPAPDGGKESM---IGRVCRQLDHAGLGASA 56 T+ + + + Sbjct: 2 KTTVAAIKMPPVMTAGNGRGEGSSNSWYSI----RAAANNTAEVRIYDEIGGWGIS---- 53 Query: 57 LFVAHEDQLALTRRHTRN--RYPVIGEPHKRGTFTXXXXXXXXXXXXXXXKPEDTLCVAP 114 E+ AL + + N + G K +A Sbjct: 54 ARWFAEELAALGQINRINLHIHSPGGAVLDGIA------IYNLLKNHPAQKTVYIDGMAC 107 Query: 115 ADMYTDDA--FFDCFHRFTDILADSQAELVLLGTRPTHPSDQYGYIIPGNAGPGAYAPVL 172 + ++ V G + Y Sbjct: 108 SMASAIAMVGNPIIMPENAMMMIHKPR-GVAGGEAEDIREYADLLDKIESVIIPIYTEKT 166 Query: 173 SFQEKPDSARALELIQQ-QALWNCGIFAFRLSFMLSHLERMGLPA--------EPAAFTE 223 + +A + Sbjct: 167 GKTPEDIAAMLAKETWMS- 225 Query: 224 L---YPGFPVRSFDQEVAERSRKAVVLRHEGEWRDLGSWDALT-----ARLADPVIGEGG 275 Sbjct: 226 ---- 281 Query: 276 IWGSSRDTHIVNDLGIPLHVIGVSGIVAAGSPEGILIAGKGEANAIKEILQQ 327 Sbjct: 282 -- 331 >PA3551_1 [M] COG0836 Mannose-1-phosphate guanylyltransferase Length = 332 Score = 77.8 bits (190), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 83/338 (24%), Positives = 133/338 (39%), Gaps = 38/338 (11%) Query: 4 ILLCGGSGQKXXXXXXXRS-KMFLELLPAPDGGKESMIGRVCRQLDHAGLGASALFVAHE 62 ++L GGSG + + K FL L G +++ + ++L G+ A L E Sbjct: 4 VILSGGSGSRLWPLSRKQYPKQFLALT-----GDDTLFQQTIKRLAFDGMQAPLLVCNKE 58 Query: 63 DQLALTRR---HTRNRYPVIGEPHKRGTFTXXXXXXXXXXXXXXXKPEDTLCVAPADMYT 119 + + + ++ EP R T ++ L + PAD Sbjct: 59 HRFIVQEQLEAQNLASQAILLEPFGRNT---APAVAIAAMKLVAEGRDELLLILPADHVI 115 Query: 120 DD--AFFDCFHRFTDILADSQAELVLLGTRPTHPSDQYGYIIPGNAG--PGAYAPVLSFQ 175 +D AF T+ A + E+VL G + P YGYI P + V SF Sbjct: 116 EDQRAFQQALALATN--AAEKGEMVLFGIPASRPETGYGYIRASADAQLPEGVSRVQSFV 173 Query: 176 EKPDSARALELIQQQAL-WNCGIFAFRLSFMLSHLERMGLPAEPAAFTEL---------- 224 EKPD ARA E + WN G+F FR S L L++ L Sbjct: 174 EKPDEARAREFVAAGGYYWNSGMFLFRASRYLEELKKHDADIYDTCLLALERSQHDGDLV 233 Query: 225 ------YPGFPVRSFDQEVAERSRKAVVLRHEGEWRDLGSWDAL---TARLADPVIGEGG 275 + P S D V E++ +A V+ W D+GSW ++ A+ A+ + +G Sbjct: 234 NIDAATFECCPDNSIDYAVMEKTSRACVVPLSAGWNDVGSWSSIWDVHAKDANGNVTKGD 293 Query: 276 IWGSSRDTHIVNDLGIPLHVIGVSGIVAAGSPEGILIA 313 + +V+ G + VIG+ IV + + ++IA Sbjct: 294 VLVHDSHNCLVHGNGKLVSVIGLEDIVVVETKDAMMIA 331 >STM2084_1 [M] COG0836 Mannose-1-phosphate guanylyltransferase Length = 355 Score = 76.6 bits (187), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 61/218 (27%), Positives = 95/218 (43%), Gaps = 22/218 (10%) Query: 137 SQAELVLLGTRPTHPSDQYGYIIPGN-AGPGAYAPVLSFQEKPDSARALELIQQ-QALWN 194 + +LV G PTH YGYI G G AYA V F EKPD A + + + WN Sbjct: 137 ANGKLVTFGIVPTHAETGYGYIRRGELIGNDAYA-VAEFVEKPDIDTAGDYFKSGKYYWN 195 Query: 195 CGIFAFRLSFMLSHLERMG---LPAEPAAFTELYPGF-------------PVRSFDQEVA 238 G+F FR S L+ L+ + A A + P P S D V Sbjct: 196 SGMFLFRASSYLNELKYLSPEIYKACEKAVGHINPDLDFIRIDKEEFMSCPSDSIDYAVM 255 Query: 239 ERSRKAVVLRHEGEWRDLGSWDAL---TARLADPVIGEGGIWGSSRDTHIVNDLGIPLHV 295 E ++ AVV+ W D+GSW +L + + + +G I+ + + + + + + Sbjct: 256 EHTQHAVVIPMSAGWSDVGSWSSLWDISNKDHQRNVLKGDIFAHACNDNYIYSEDMFISA 315 Query: 296 IGVSGIVAAGSPEGILIAGKGEANAIKEILQQAAEEPR 333 IGVS +V + + +L+A K +K+I+ R Sbjct: 316 IGVSNLVIVQTTDALLVANKDTVQDVKKIVDYLKRNDR 353 >mlr5802_1 [M] COG0836 Mannose-1-phosphate guanylyltransferase Length = 338 Score = 71.6 bits (174), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 66/243 (27%), Positives = 99/243 (40%), Gaps = 48/243 (19%) Query: 106 PEDTLCVAPADMYTDD--AFFDCFHRFTDILADSQAELVLLGTRPTHPSDQYGYIIPGNA 163 P+ LCV PAD D AF +R I Q + LG P P +GYI Sbjct: 108 PDAVLCVFPADHMIGDLPAFQTAMNRA--IEQALQGRIATLGITPDRPDTAFGYI----- 160 Query: 164 GPGAYAPVLSFQEKPDSARALELI-QQQALWNCGIFAFRLSFML---------------- 206 V+ F EKP++ A + ++ WN GIF F+ ML Sbjct: 161 -EADGEKVVRFVEKPNAETAKSYVASKRFFWNAGIFCFKAQVMLDEMAAHCPQVIDAVLD 219 Query: 207 -------SHLERMGLPAEPAAFTELYPGFPVRSFDQEVAERSRKAVVLRHEGEWRDLGSW 259 SH E + A +E + P S D V E++ V+ E W D+GSW Sbjct: 220 SYDNARVSHGEHV---ASVELSSEHFAMAPRISLDHAVMEKTHNLAVVPCEMGWNDIGSW 276 Query: 260 DALTARLADPVIGEGGIWGSSRDTHIVNDLGIPLH-------VIGVSGIVAAGSPEGILI 312 +A +A+ V +G D H+V+ +G + +G+S +V SP+ +L+ Sbjct: 277 NA----MAELVAADGSGNRIRGDVHVVDTVGSYISSDKRVIGTVGISDLVIVDSPDALLV 332 Query: 313 AGK 315 A + Sbjct: 333 ASR 335 >mlr7607_1 [M] COG0836 Mannose-1-phosphate guanylyltransferase Length = 379 Score = 67.4 bits (163), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 56/215 (26%), Positives = 90/215 (41%), Gaps = 25/215 (11%) Query: 141 LVLLGTRPTHPSDQYGYIIPGNAGPGAYAPVLSFQEKPDSARALELIQQ-QALWNCGIFA 199 LV+ G +PT P YGYI +A G + V F EKPD A A ++ WN GIF Sbjct: 143 LVVFGIKPTQPETGYGYI-EVSAEKGGVSDVSRFVEKPDLATAQGYLEAGNFYWNTGIFL 201 Query: 200 FRLSFMLS-------HLERMGLPAEPAAFTEL---------YPGFPVRSFDQEVAERSRK 243 FR + M + R A AA ++L Y P S D + ER+R Sbjct: 202 FRAAAMRDAFAVFQPDIWRATEAAYKAATSDLSGLYMPLDLYAAIPSNSIDYAIMERARD 261 Query: 244 AVVLRHEGEWRDLGSWDAL-----TARLADPVIGEGGIWGSSRDTHIVNDLGIPLHVIGV 298 ++ W DLGSW +L + ++G+ + + + G L IG+ Sbjct: 262 IAMVPAGFRWNDLGSWQSLLDVGPADDQGNVIVGD--VVAIDCENSYIRSEGRLLSAIGM 319 Query: 299 SGIVAAGSPEGILIAGKGEANAIKEILQQAAEEPR 333 + + + +A + +K++++Q + R Sbjct: 320 KDVAIVSTADATFVAPVSHSQHVKKVVEQLEKSGR 354 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.139 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,546,345 Number of extensions: 1422070 Number of successful extensions: 4632 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 4615 Number of HSP's successfully gapped: 17 Length of query: 438 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 329 Effective length of database: 25,807,025 Effective search space: 8490511225 Effective search space used: 8490511225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 117 (49.7 bits)