BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0535.0002.p (173 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0676 [I] COG1183 Phosphatidylserine synthase 142 3e-34 PA4693 [I] COG1183 Phosphatidylserine synthase 78 6e-15 AGc1962 [I] COG1183 Phosphatidylserine synthase 78 6e-15 BMEI1490 [I] COG1183 Phosphatidylserine synthase 77 1e-14 SMc00552 [I] COG1183 Phosphatidylserine synthase 73 2e-13 >CAC0676 [I] COG1183 Phosphatidylserine synthase Length = 211 Score = 142 bits (358), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 13/188 (6%), Positives = 27/188 (14%), Gaps = 26/188 (13%) Query: 1 MVKKSIPNVLTFLNLSFGVLAILEIVGGS------YFISGVFLICAAVVDRYDGRIARFL 54 M K +LT L + +S + Sbjct: 1 MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANT 60 Query: 55 NVSSTLGKELDSLADLVSFGVAPALLIF--CKF--NFLNLGHIKGIGICVLLLYVISGSY 110 + + + G+ + V + Sbjct: 61 PAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGE------VPEDRAALEAL 114 Query: 111 RLAKYNIGHFDNVFIGMPITVAGFILAVYSL------AAPINSAFTMGSVVLLLFLACFM 164 + T+A N LL + Sbjct: 115 PGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGK-NVEQV---EEKLLKVVPAE 170 Query: 165 VSKYKFNK 172 + Sbjct: 171 FKVDCHHW 178 >PA4693 [I] COG1183 Phosphatidylserine synthase Length = 271 Score = 78.2 bits (191), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 48/125 (38%), Positives = 70/125 (56%), Gaps = 7/125 (5%) Query: 6 IPNVLTFLNLSFGVLAILEIVGGSYFISGVFLICAAVVDRYDGRIARFLNVSSTLGKELD 65 +PN+ T L G AI+ + G + + + + A V+D DGR+AR N S G E D Sbjct: 43 LPNLFTTAALFSGFYAIINGMSGQFANAAIAIFVAMVLDGLDGRVARLTNTQSAFGAEYD 102 Query: 66 SLADLVSFGVAPALLIFCKFNFLNLGHIKGIGICVLLLYVISGSYRLAKYN--IGHFDN- 122 SL+D+V+FGVAPAL+ F LG + +G V +YV + RLA++N +G D Sbjct: 103 SLSDMVAFGVAPALVAFE----WALGSLGKVGWMVAFIYVAGAALRLARFNTQVGKADKR 158 Query: 123 VFIGM 127 FIG+ Sbjct: 159 YFIGL 163 >AGc1962 [I] COG1183 Phosphatidylserine synthase Length = 290 Score = 78.2 bits (191), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 40/112 (35%), Positives = 70/112 (62%), Gaps = 4/112 (3%) Query: 6 IPNVLTFLNLSFGVLAILEIVGGSYFISGVFLICAAVVDRYDGRIARFLNVSSTLGKELD 65 +PN++T L + G+ + + G + ++ ++ AA +D DGR+AR + +S G+++D Sbjct: 37 VPNLITVLAICAGLSGVRLAIEGRFELAVGMVLLAAFLDGVDGRVARMMKATSKFGEQMD 96 Query: 66 SLADLVSFGVAPALLIFCKFNFLNLGHIKGIGICVLLLYVISGSYRLAKYNI 117 SLAD+V+FGVAPAL+++ L + IG L+YVI+ RLA++N+ Sbjct: 97 SLADIVNFGVAPALVVYIYL----LDQARSIGWIGALIYVIAAGLRLARFNV 144 >BMEI1490 [I] COG1183 Phosphatidylserine synthase Length = 211 Score = 77.4 bits (189), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 10/144 (6%), Positives = 24/144 (16%), Gaps = 14/144 (9%) Query: 6 IPNVLTFLNLSFGVLAILEIVGGSYFISGVFLICAAVVDRYDGRIARFLNVSSTLGKELD 65 N T + G + + + I + E+ Sbjct: 46 SVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNGEVP 105 Query: 66 SLADLVSFGVAPALLIFCKFNFLNLGHIKGIGICVLLLYVISGSYRLAKYNI-------- 117 + + L G + ++ R Sbjct: 106 EDRAALEALPGVGRKTAN----VVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEE 161 Query: 118 GHFDNVFIGMPITVAGF--ILAVY 139 V + + + Y Sbjct: 162 KLLKVVPAEFKVDCHHWLILHGRY 185 >SMc00552 [I] COG1183 Phosphatidylserine synthase Length = 289 Score = 73.2 bits (178), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 54/174 (31%), Positives = 91/174 (52%), Gaps = 16/174 (9%) Query: 6 IPNVLTFLNLSFGVLAILEIVGGSYFISGVFLICAAVVDRYDGRIARFLNVSSTLGKELD 65 +PN++T L + G+ + + ++ ++ AA +D DGRIAR L +S+ G ++D Sbjct: 42 VPNMITVLAICAGLSGVRLAFENRFELAVAMVLLAAFLDGIDGRIARLLKATSSFGVQMD 101 Query: 66 SLADLVSFGVAPALLIFCKFNFLNLGHIKGIGICVLLLYVISGSYRLAKYNIGHFDNV-- 123 SLAD+++FGVAPAL+++ FL L + +G L+Y I+ RLA++N+ V Sbjct: 102 SLADIINFGVAPALVLYV---FL-LDQARSLGWIAALIYAIAVGLRLARFNVMAERQVKA 157 Query: 124 ------FIGMPITVAGF--ILAVY--SLAAPINSAFTMGSVVLLLFLACFMVSK 167 F+G+P +L VY L F + + V + +A MVS+ Sbjct: 158 SWQSEYFVGVPAPAGAMLVLLPVYLGLLGLAPERTFALIASVYTVLIAFLMVSR 211 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.332 0.147 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,759,201 Number of extensions: 435962 Number of successful extensions: 1416 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 1417 Number of HSP's successfully gapped: 7 Length of query: 173 Length of database: 38,378,431 Length adjustment: 99 Effective length of query: 74 Effective length of database: 26,960,365 Effective search space: 1995067010 Effective search space used: 1995067010 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 112 (47.8 bits)