BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0535.0001.p (408 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3168 [C] COG0281 Malic enzyme 390 e-108 BH0399 [C] COG0281 Malic enzyme 381 e-106 BS_ytsJ [C] COG0281 Malic enzyme 378 e-105 MA1735 [C] COG0281 Malic enzyme 376 e-104 mlr5329_1 [C] COG0281 Malic enzyme 338 1e-92 >BH3168 [C] COG0281 Malic enzyme Length = 410 Score = 390 bits (1003), Expect = e-108, Method: Compositional matrix adjust. Identities = 210/389 (53%), Positives = 263/389 (67%), Gaps = 4/389 (1%) Query: 18 DEIMLLHK--GGKLETTLKNPIRTKEDLSKVYTPGVAEVCQAIAQDQAQAYELTTKKNTV 75 +E + +H+ GKLE+ K P+R DLS Y+PGVAE C+ I +D YE T K N V Sbjct: 6 EEALHIHRENQGKLESKSKVPVRNARDLSLAYSPGVAEPCKEIFEDVETVYEYTMKGNMV 65 Query: 76 AVVSDGTAVLGLGDIGPRAAMPVMEGKAALFKQFANVDAVPICLDTKGTEEIIAIVKALA 135 AVVS+GTAVLGLG+IGP A++PVMEGKA LFK FA VDA PICL T ++I+ VK L Sbjct: 66 AVVSNGTAVLGLGNIGPEASLPVMEGKAVLFKSFAGVDAFPICLRTTDVDQIVETVKLLE 125 Query: 136 PTFGGINLEDISSPRCFEIEKRLRKELDIPVFHDDQHGTAIVVLAGLLNALKVCGKNIKD 195 PTFGG+NLEDI++P CFEIE+RL+KE +IPVFHDDQHGTAIV LAGL+NALK+ GK + + Sbjct: 126 PTFGGVNLEDIAAPSCFEIEERLKKETNIPVFHDDQHGTAIVTLAGLINALKLTGKRMSE 185 Query: 196 VKIVVNGIGAAGISVSKMLLHAGAVNLIGVDREGALNRQVAYSRTHWN-EYARMTNPCLE 254 +K+V NG GAAGI++ K++ G ++I D +GA+ Y E A+ TN Sbjct: 186 IKVVANGAGAAGIAIIKLMQRMGVRDIIMCDSKGAIYDGRPYGMNDMKAEVAKWTNRDKI 245 Query: 255 TGQLSDVIKGADVFIGVSAPDVLKLEDVLNMAKDPIVFAMANPTPEIDPAIA-APHVRVM 313 G L DV+KGADVFIGVS L E + NM DPI+FAMANP PEI P A A +V+ Sbjct: 246 DGGLGDVLKGADVFIGVSVAGALTKEMIENMNDDPIIFAMANPVPEIMPEEAKAAGAKVI 305 Query: 314 ATGRSDYPNQINNVLCFPGIFRGALDCEATDINDEMKLAAAQAISSVIDAADLHETYIIP 373 TGRSD+PNQ+NNVL FPGIFRGALD AT IN+EMK+AA AI+ ++ DL Y+IP Sbjct: 306 GTGRSDFPNQVNNVLAFPGIFRGALDARATHINEEMKVAAVHAIADLVAEEDLDPDYVIP 365 Query: 374 DAFDPXXXXXXXXXXXXXXFNSGAARKKL 402 FDP +G AR+K+ Sbjct: 366 APFDPRVAPAVAAAVAKTAMETGVARRKV 394 >BH0399 [C] COG0281 Malic enzyme Length = 397 Score = 381 bits (979), Expect = e-106, Method: Compositional matrix adjust. Identities = 209/366 (57%), Positives = 265/366 (72%), Gaps = 5/366 (1%) Query: 16 LLDEIMLLHK--GGKLETTLKNPIRTKEDLSKVYTPGVAEVCQAIAQDQAQAYELTTKKN 73 L +E + LH+ GKLE K I ++DLS VYTPGVA+VC+ IA+ + LT++ N Sbjct: 6 LKEEAIALHQKHSGKLEIVGKMEITDEKDLSLVYTPGVADVCKQIAEQPDKVNSLTSRGN 65 Query: 74 TVAVVSDGTAVLGLGDIGPRAAMPVMEGKAALFKQFANVDAVPICLDTKGTEEIIAIVKA 133 VA+V+DGTAVLGLG+IGP+AAMPVMEGKA LFK+FAN+D P+CL+T+ T+EI+ I+KA Sbjct: 66 MVAIVTDGTAVLGLGNIGPKAAMPVMEGKALLFKKFANIDGFPVCLNTQDTDEIVQIIKA 125 Query: 134 LAPTFGGINLEDISSPRCFEIEKRLRKELDIPVFHDDQHGTAIVVLAGLLNALKVCGKNI 193 L+PTF GINLEDI++PRCFEIE+RL++ELDIPVFHDDQHGTAIVVLA L NALKV K Sbjct: 126 LSPTFAGINLEDIAAPRCFEIEERLKEELDIPVFHDDQHGTAIVVLAALTNALKVVNKLN 185 Query: 194 KDVKIVVNGIGAAGISVSKMLLHAGAVNLIGVDREGALNRQVAYSRTHWNEYARMTNPCL 253 + +K+V+NG GAAG +++ +LLHAG ++ V EG + + A+ + A +TN Sbjct: 186 QPIKMVINGAGAAGTAIANLLLHAGFKDITLVSLEGVVAKGEAWLNPMQAKMAEVTNLAG 245 Query: 254 ETGQLSDVIKGADVFIGVSAPDVLKLEDVLNMAKDPIVFAMANPTPEIDP--AIAAPHVR 311 G L D I ADVFIGVS P +K E + MAKDPIV A+ANP PEI P AI A Sbjct: 246 VKGTLKDAITDADVFIGVSGPGAMKKEHIQAMAKDPIVLALANPIPEIYPEEAIEA-GAA 304 Query: 312 VMATGRSDYPNQINNVLCFPGIFRGALDCEATDINDEMKLAAAQAISSVIDAADLHETYI 371 V+ TGRSDYPNQ+NN+L FPGIFRGALD A I EMK+AAA AI+ V+ +++ I Sbjct: 305 VVGTGRSDYPNQVNNLLAFPGIFRGALDAGAKTITMEMKIAAAYAIADVLSDKEVNANMI 364 Query: 372 IPDAFD 377 IPDA D Sbjct: 365 IPDALD 370 >BS_ytsJ [C] COG0281 Malic enzyme Length = 410 Score = 378 bits (971), Expect = e-105, Method: Compositional matrix adjust. Identities = 208/390 (53%), Positives = 263/390 (67%), Gaps = 4/390 (1%) Query: 14 VELLDEIMLLHK--GGKLETTLKNPIRTKEDLSKVYTPGVAEVCQAIAQDQAQAYELTTK 71 + L +E + LHK GKLE+ K +R +DLS Y+PGVAE C+ I +D + Y+ T K Sbjct: 1 MSLREEALHLHKVNQGKLESKSKVEVRNAKDLSLAYSPGVAEPCKDIHEDINKVYDYTMK 60 Query: 72 KNTVAVVSDGTAVLGLGDIGPRAAMPVMEGKAALFKQFANVDAVPICLDTKGTEEIIAIV 131 N VAVV+DGTAVLGLG+IGP AA+PVMEGKA LFK FA VDA PI L+T ++I+ V Sbjct: 61 GNMVAVVTDGTAVLGLGNIGPEAALPVMEGKAVLFKSFAGVDAFPIALNTNDVDKIVETV 120 Query: 132 KALAPTFGGINLEDISSPRCFEIEKRLRKELDIPVFHDDQHGTAIVVLAGLLNALKVCGK 191 K L PTFGG+NLEDI++P CF IE+RL+KE +IPVFHDDQHGTAIV +AGL+NALK+ GK Sbjct: 121 KLLEPTFGGVNLEDIAAPNCFIIEERLKKETNIPVFHDDQHGTAIVTVAGLVNALKLSGK 180 Query: 192 NIKDVKIVVNGIGAAGISVSKMLLHAGAVNLIGVDREGALNRQVAYSRTH-WNEYARMTN 250 ++ +K+V NG GAAGI++ K+L H G +++ D +GA+ NE A+ TN Sbjct: 181 SMSSIKVVANGAGAAGIAIIKLLHHYGVRDIVMCDSKGAIYEGRPNGMNDVKNEVAKFTN 240 Query: 251 PCLETGQLSDVIKGADVFIGVSAPDVLKLEDVLNMAKDPIVFAMANPTPEIDPAIA-APH 309 + G L DVI ADVFIGVS L E V +MAKDPI+FAMANP PEI P A Sbjct: 241 QDRKDGSLKDVIVDADVFIGVSVAGALTKEMVQSMAKDPIIFAMANPNPEIMPEDAREAG 300 Query: 310 VRVMATGRSDYPNQINNVLCFPGIFRGALDCEATDINDEMKLAAAQAISSVIDAADLHET 369 V+ TGRSD+PNQ+NNVL FPGIFRGALD AT IN+EMK+AA +AI+S++ +L Sbjct: 301 ASVVGTGRSDFPNQVNNVLAFPGIFRGALDVRATHINEEMKIAAVEAIASLVSEDELSAD 360 Query: 370 YIIPDAFDPXXXXXXXXXXXXXXFNSGAAR 399 Y+IP FD +G AR Sbjct: 361 YVIPAPFDKRVAPAVAKAVAKAAMETGVAR 390 >MA1735 [C] COG0281 Malic enzyme Length = 390 Score = 376 bits (966), Expect = e-104, Method: Compositional matrix adjust. Identities = 201/391 (51%), Positives = 259/391 (66%), Gaps = 16/391 (4%) Query: 23 LHK--GGKLETTLKNPIRTKEDLSKVYTPGVAEVCQAIAQDQAQAYELTTKKNTVAVVSD 80 +H+ GG LE K +RT DLS YTPGVAE C+ I ++ + Y T KKNTVAVV+D Sbjct: 1 MHRRLGGVLEVESKVRLRTIHDLSVAYTPGVAEPCRKIKENPSLVYLYTIKKNTVAVVTD 60 Query: 81 GTAVLGLGDIGPRAAMPVMEGKAALFKQFANVDAVPICLDTKGTEEIIAIVKALAPTFGG 140 G+AVLGLG+IGP AA+PVMEGKA +FK+FA +DA PICL+T+ TEE+I VK LAP FGG Sbjct: 61 GSAVLGLGNIGPCAALPVMEGKAIIFKEFAGIDAFPICLNTQDTEEVIKAVKYLAPAFGG 120 Query: 141 INLEDISSPRCFEIEKRLRKELDIPVFHDDQHGTAIVVLAGLLNALKVCGKNIKDVKIVV 200 INLEDIS+PRCFEIE RLR+ELDIPV HDDQHG AIVV AGLLNALK+ K + ++KI++ Sbjct: 121 INLEDISAPRCFEIESRLREELDIPVIHDDQHGAAIVVFAGLLNALKIVKKELGELKILI 180 Query: 201 NGIGAAGISVSKMLLHAGA--VNLIGVDREGALNRQVAYSRTHWN------EYARMTNPC 252 G+GAAG+++ + L+ AGA ++ D +G + Y + E A +TNP Sbjct: 181 TGLGAAGVAIFRFLVRAGADPAKILTCDSQG-----IVYEGREKDMNPIKAEIATLTNPG 235 Query: 253 LETGQLSDVIKGADVFIGVSAPDVLKLEDVLNMAKDPIVFAMANPTPEIDP-AIAAPHVR 311 G L + G D+FIGVS ++ E + +MA D IV AMANPTPEI P A R Sbjct: 236 KLKGGLKEAFPGTDLFIGVSVGGIVTEEMISSMANDAIVMAMANPTPEIMPDAAKRAGAR 295 Query: 312 VMATGRSDYPNQINNVLCFPGIFRGALDCEATDINDEMKLAAAQAISSVIDAADLHETYI 371 ++ATGRSD+PNQ+NN L FPG+F+GAL A I EM++AAA A+++V+ +L E YI Sbjct: 296 IVATGRSDFPNQLNNCLSFPGVFKGALGTCARKITPEMEMAAAHALANVVSVGELSEDYI 355 Query: 372 IPDAFDPXXXXXXXXXXXXXXFNSGAARKKL 402 IP+ D S AAR+ L Sbjct: 356 IPEPLDKHVVPAVAKAVANASLESCAARRSL 386 >mlr5329_1 [C] COG0281 Malic enzyme Length = 434 Score = 338 bits (866), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 187/404 (46%), Positives = 253/404 (62%), Gaps = 23/404 (5%) Query: 19 EIMLLHKGGKLETTLKNPIRTKEDLSKVYTPGVAEVCQAIAQDQAQAYELTTKKNTVAVV 78 E + + GKLE P+ T+ DLS Y+PGVA +AIA+D ++A++ TT+ N VAV+ Sbjct: 19 EFHAMGRPGKLEIVATKPMTTQRDLSLAYSPGVAVPVRAIAEDPSRAFDYTTRGNMVAVI 78 Query: 79 SDGTAVLGLGDIGPRAAMPVMEGKAALFKQFANVDAVPICLDTKGTEEIIAIVKALAPTF 138 S+GTA+LGLG++G A+ PVMEGKA LFK+FA+VD++ + +DT+ +E I V+ L P+F Sbjct: 79 SNGTAILGLGNLGALASKPVMEGKAVLFKRFADVDSIDLEVDTEDADEFINCVRFLGPSF 138 Query: 139 GGINLEDISSPRCFEIEKRLRKELDIPVFHDDQHGTAIVVLAGLLNALKVCGKNIKDVKI 198 GGINLEDI +P CF IE+RLR+ +DIPVFHDDQHGTAI+ AGL+NAL++ G+++K ++ Sbjct: 139 GGINLEDIKAPECFIIEQRLRELMDIPVFHDDQHGTAIISAAGLINALEITGRDMKTTRL 198 Query: 199 VVNGIGAAGISVSKMLLHAGAV--NLIGVDREGALNRQVAYSRTHWNEYARMTNPCLETG 256 V NG GAAGI+ +++ G N+I D +G + + W + E Sbjct: 199 VCNGAGAAGIACIELMKAMGFAPENIILCDTKGVVFQGRTEGMNQWKSAHAVKT---EAR 255 Query: 257 QLSDVIKGADVFIGVSAPDVLKLEDVLNMAKDPIVFAMANPTPEIDPAIAAPHVR---VM 313 L+D + GADVF+G+SA L V +MAK+PI+FAMANP PEI P A +R +M Sbjct: 256 SLADALDGADVFLGLSAKGALTTAMVQSMAKNPIIFAMANPDPEITPEEVA-EIRTDAIM 314 Query: 314 ATGRSDYPNQINNVLCFPGIFRGALDCEATDINDEMKL--------AAAQAISSVIDAA- 364 ATGRSDYPNQ+NNVL FP IFRGALD AT IN++MK+ A Q + + AA Sbjct: 315 ATGRSDYPNQVNNVLGFPYIFRGALDVRATTINEDMKIAAARALAELARQDVPDDVAAAY 374 Query: 365 -----DLHETYIIPDAFDPXXXXXXXXXXXXXXFNSGAARKKLL 403 YIIP FDP SG ARK +L Sbjct: 375 QGNRPKFGPNYIIPVPFDPRLISAIPLAVAKAAMESGVARKPIL 418 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,178,847 Number of extensions: 1147745 Number of successful extensions: 3917 Number of sequences better than 1.0e-05: 41 Number of HSP's gapped: 3832 Number of HSP's successfully gapped: 42 Length of query: 408 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 299 Effective length of database: 25,807,025 Effective search space: 7716300475 Effective search space used: 7716300475 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)