BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0534.0023.p (283 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yqjO [E] COG0345 Pyrroline-5-carboxylate reductase 131 1e-30 lin0414 [E] COG0345 Pyrroline-5-carboxylate reductase 126 3e-29 BS_proH [E] COG0345 Pyrroline-5-carboxylate reductase 122 7e-28 aq_1664 [I] COG0439 Biotin carboxylase 122 9e-28 aq_1663 [N] COG1344 Flagellin and related hook-associated proteins 122 9e-28 >BS_yqjO [E] COG0345 Pyrroline-5-carboxylate reductase Length = 346 Score = 131 bits (329), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 15/270 (5%), Positives = 39/270 (14%), Gaps = 10/270 (3%) Query: 15 QRIHFIGGGQMAEAIIRAVTANQTIAAGRISVTD-INEARLQVLTNAYGVDTGRSQETYL 73 Q + + + + Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 74 AEAELIVLAVRPQDALDGLGQAITRLAAPDAVIVSIVAGLTLEKLAGVFGAGRPIVRVIP 133 + + + G+ ++ + + Sbjct: 62 QGLTETYVGNWLAKHGSREKL--IIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLK 119 Query: 134 NTLTDTGYGYSGVVLNANASREQV---DEFLLGFGKVQYMEETFLDIFTGYGVAGPNYIY 190 TD Y + L + G + Sbjct: 120 RLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVS 179 Query: 191 YFIESLVDAGVLAGVPXXXPREQAWNVALENMAGAVLMLRQTGLHPRQLLDINNSPGGVG 250 V + + V ++N + + GL + Sbjct: 180 NETAFGVMRYLHLADK----HDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSC 235 Query: 251 MHGLYELNNSDFAAGLQRSVMAAVKRTTEL 280 + A + R T Sbjct: 236 LGFGTLTGKYLNGAKPAGARNTLFSRFTRY 265 >lin0414 [E] COG0345 Pyrroline-5-carboxylate reductase Length = 346 Score = 126 bits (317), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 11/270 (4%), Positives = 33/270 (12%), Gaps = 9/270 (3%) Query: 15 QRIHFIGGGQMAEAIIRAVTANQTIAAGRISVTDINEARLQVLTNAY-GVDTGRSQETYL 73 Q + + + ++ A Q + Sbjct: 2 QYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPET 61 Query: 74 AEAELIVLAVRPQDALDGLGQAITRLAAPDAVIVSIVAGLTLEKLAGVFGAGRPIVRVIP 133 + + I + + L + Sbjct: 62 QGLTETYVGNWLAKHGSRE-KLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 134 NTLTDTGYGYSGVVLNANASREQ---VDEFLLGFGKVQYMEETFLDIFTGYGVAGPNYIY 190 + + + + + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 191 YFIESLVDAGVLAGVPXXXPREQAWNVALENMAGAVLMLRQTGLHPRQLLDINNSPGGVG 250 ++ LA + Q + Sbjct: 181 ETAFGVMRYLHLADKH----DLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCL 236 Query: 251 MHGLYELNNSDFAAGLQRSVMAAVKRTTEL 280 G + A + T Sbjct: 237 GFGTLTGKYLNGAKPAGARNTLFSRFTRYS 266 >BS_proH [E] COG0345 Pyrroline-5-carboxylate reductase Length = 346 Score = 122 bits (306), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 23/251 (9%), Positives = 42/251 (16%), Gaps = 9/251 (3%) Query: 14 EQRIHFIGGGQMAEAIIRAVTANQTIAAGRISVTD-INEARLQVLTNAYGVDTGRSQETY 72 G Q +EA A + + + T + Sbjct: 16 LGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAK 75 Query: 73 LAEAELIVLAVRPQDALDGLGQAITRLAAPDAVIVSIVAGLTLEKLAGVFGAGRPIVRVI 132 E +++A + + I A K V Sbjct: 76 HGSREKLIIASKVSGPSRNNDKGIRPDQ-ALDRKNIREALHDSLKRLQTDYLDLYQVHWP 134 Query: 133 PNTLTDTGYGYSGVVLNANASRE--QVDEFLLG-FGKVQYMEETFLDIFTGYGVAGPNYI 189 G +A A +D + G Sbjct: 135 QRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLAD 194 Query: 190 YYFIESLVDAGVLAGVPXXXPREQAWNVALENMAGAVLMLRQTGLHPRQLLDINNSPGGV 249 + + +V + E + L L G Sbjct: 195 KHDLPRIVTIQNPYSLLNRSFE----VGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAK 250 Query: 250 GMHGLYELNNS 260 L + Sbjct: 251 PAGARNTLFSR 261 >aq_1664 [I] COG0439 Biotin carboxylase Length = 265 Score = 122 bits (305), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 81/279 (29%), Positives = 145/279 (51%), Gaps = 26/279 (9%) Query: 12 LIEQRIHFIGGGQMAEAIIRAVTANQTIAAGRISVTDINEARLQVLTN---AYGVDTGRS 68 ++ R+ +G G M +A A+ ++ + I VTD + + + T A+ D Sbjct: 5 ILTMRVGIVGFGNMGQAF--ALCFSKKLGKENIIVTDKVQEKRNLATEMGIAFASDV--- 59 Query: 69 QETYLAE-AELIVLAVRPQDALDGLGQAITRLAAPDAVIVSIVAGLTLEKLAGVFGAGRP 127 +LA+ ++++++AV+P+D+ + + +L +I+SI+AG+++EK+ + G + Sbjct: 60 --KFLADNSDVVLVAVKPKDS----QEVLQKLKDYKGIILSIMAGVSIEKMEKILGKDKK 113 Query: 128 IVRVIPNTLTDTGYGYSGVVLNANAS---REQVDEFLLGFGKVQYMEETFLDIFTGYGVA 184 IVRV+PN G G + N N S R +V+E LL G + +EE D FT + Sbjct: 114 IVRVMPNVNVAVGSGVMAITDNGNLSEEERSKVEELLLSCGTLYRIEERLFDAFTALAGS 173 Query: 185 GPNYIYYFIESLVDAGVLAGVPXXXPREQAWNVALENMAGAVLMLRQTGLHPRQLLDINN 244 GP +++ FI DA LAGV EQA +AL+ + G+ +L++ ++P +L+ Sbjct: 174 GPAFVFSFI----DALALAGVHQGFSYEQALRIALDTVMGSAKLLKEFQVNPNELIAKVT 229 Query: 245 SPGGVGMHGLYELNNSDFAAGLQRSVMAAVKRTTELGAK 283 SPGG + G+ L G + +VM + RT++ K Sbjct: 230 SPGGTTIEGIKYLEEK----GFKGTVMECINRTSQKAKK 264 >aq_1663 [N] COG1344 Flagellin and related hook-associated proteins Length = 265 Score = 122 bits (305), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 81/279 (29%), Positives = 145/279 (51%), Gaps = 26/279 (9%) Query: 12 LIEQRIHFIGGGQMAEAIIRAVTANQTIAAGRISVTDINEARLQVLTN---AYGVDTGRS 68 ++ R+ +G G M +A A+ ++ + I VTD + + + T A+ D Sbjct: 5 ILTMRVGIVGFGNMGQAF--ALCFSKKLGKENIIVTDKVQEKRNLATEMGIAFASDV--- 59 Query: 69 QETYLAE-AELIVLAVRPQDALDGLGQAITRLAAPDAVIVSIVAGLTLEKLAGVFGAGRP 127 +LA+ ++++++AV+P+D+ + + +L +I+SI+AG+++EK+ + G + Sbjct: 60 --KFLADNSDVVLVAVKPKDS----QEVLQKLKDYKGIILSIMAGVSIEKMEKILGKDKK 113 Query: 128 IVRVIPNTLTDTGYGYSGVVLNANAS---REQVDEFLLGFGKVQYMEETFLDIFTGYGVA 184 IVRV+PN G G + N N S R +V+E LL G + +EE D FT + Sbjct: 114 IVRVMPNVNVAVGSGVMAITDNGNLSEEERSKVEELLLSCGTLYRIEERLFDAFTALAGS 173 Query: 185 GPNYIYYFIESLVDAGVLAGVPXXXPREQAWNVALENMAGAVLMLRQTGLHPRQLLDINN 244 GP +++ FI DA LAGV EQA +AL+ + G+ +L++ ++P +L+ Sbjct: 174 GPAFVFSFI----DALALAGVHQGFSYEQALRIALDTVMGSAKLLKEFQVNPNELIAKVT 229 Query: 245 SPGGVGMHGLYELNNSDFAAGLQRSVMAAVKRTTELGAK 283 SPGG + G+ L G + +VM + RT++ K Sbjct: 230 SPGGTTIEGIKYLEEK----GFKGTVMECINRTSQKAKK 264 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,290,705 Number of extensions: 814670 Number of successful extensions: 1722 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 1689 Number of HSP's successfully gapped: 14 Length of query: 283 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 178 Effective length of database: 26,268,361 Effective search space: 4675768258 Effective search space used: 4675768258 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)