BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0534.0018.p (160 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yxeP [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 123 1e-28 BS_hipO [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 115 4e-26 CAC0921 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 102 3e-22 PH0722 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxyp... 98 5e-21 mlr3583 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxy... 93 2e-19 >BS_yxeP [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 380 Score = 123 bits (308), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 62/157 (39%), Positives = 90/157 (57%) Query: 1 METVVLSVTRINGGHTWNVLPERVELEGTVRTYNEEIRASMPEKITRXXXXXXXXXXXXX 60 ++ V+S+TR+ G +WNV+P++ E+EGTVRT+ +E R ++PE + R Sbjct: 224 LQNAVVSITRVQAGTSWNVIPDQAEMEGTVRTFQKEARQAVPEHMRRVAEGIAAGYGAQA 283 Query: 61 RLHWAPGPPATINDGVWADFSKEIAGQAGYEVHDIPPQMGGEDFALYLQQIPGAFVNIGT 120 W P P+ NDG + + + E A + GY+ GGEDFALY ++IPG FV +GT Sbjct: 284 EFKWFPYLPSVQNDGTFLNAASEAAARLGYQTVHAEQSPGGEDFALYQEKIPGFFVWMGT 343 Query: 121 GPAYALHHPRFDVDEAALLPAARYFALLAEQALVQLK 157 HHP F +DE AL A++YFA LA L +K Sbjct: 344 NGTEEWHHPAFTLDEEALTVASQYFAELAVIVLETIK 380 >BS_hipO [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 416 Score = 115 bits (287), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 56/160 (35%), Positives = 87/160 (54%), Gaps = 1/160 (0%) Query: 1 METVVLSVTRINGGHTWNVLPERVELEGTVRTYNEEIRASMPEKITRXXXXXXXXXXXXX 60 +++ +L+V +INGG TWNV+P+ V +EGTVRT++ E+R + ++ Sbjct: 254 LQSAILTVGKINGGSTWNVIPDTVVIEGTVRTFDSEVRNQVKQRFFAVTEQISAAFSLKA 313 Query: 61 RLHWAPGPPATINDGVWADFSKEIAGQAGYEVHDIPPQMGGEDFALYLQQIPGAFVNIGT 120 + W GPP ND ++ A +A +V D P GEDFA YL+ IPG+F GT Sbjct: 314 NVKWHSGPPPLCNDEAITGLVRDAAHKAKLQVIDPAPSTAGEDFAYYLEHIPGSFAFFGT 373 Query: 121 GPAYALHHPRFDVDEAALLPAARYFALLAEQALVQLKERA 160 + HHP F +DE A++ A+ YF + + L+ E + Sbjct: 374 DGDHDWHHPAFTIDETAIIKAS-YFLYESAKRLLDSNEES 412 >CAC0921 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 382 Score = 102 bits (253), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 53/155 (34%), Positives = 77/155 (49%) Query: 1 METVVLSVTRINGGHTWNVLPERVELEGTVRTYNEEIRASMPEKITRXXXXXXXXXXXXX 60 +E+VV+S+TR N G TWNV+ + E+EGTVRT+N IR + + R Sbjct: 227 IESVVISITRFNSGTTWNVISDSAEMEGTVRTFNNNIREEIRNLMERTAKGIARALGGKI 286 Query: 61 RLHWAPGPPATINDGVWADFSKEIAGQAGYEVHDIPPQMGGEDFALYLQQIPGAFVNIGT 120 W P ND + D E A GY+ + +GGEDF+ Y +P FV +G Sbjct: 287 EFKWFSYSPVLENDVRFKDLCIEAAEDNGYKYVEAKRNLGGEDFSFYQTVVPSFFVWMGV 346 Query: 121 GPAYALHHPRFDVDEAALLPAARYFALLAEQALVQ 155 H +++ E AL+ AA YF+ LA + L + Sbjct: 347 DGTEEWHKANYNLKEDALMVAANYFSDLALKVLSK 381 >PH0722 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 388 Score = 98.2 bits (243), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 52/143 (36%), Positives = 77/143 (53%), Gaps = 5/143 (3%) Query: 1 METVVLSVTRINGGHTWNVLPERVELEGTVRTYNEEIRASMPEKITRXXXXXXXXXXXXX 60 +E+ V++V +I GG +NV+P+ VELEGT R + +E+ + +I Sbjct: 230 LESAVVTVGKIQGGTAFNVIPQYVELEGTFRFFTQELGKFLERRIREIIENTAKAHNCKA 289 Query: 61 RLHWAPGPPATINDGVWADFSKEIAGQAGYEVHDIPPQMGGEDFALYLQQIPGAFVNIG- 119 ++ P TIND F E A G +V ++ +GGEDFA YL+++PGAF+ +G Sbjct: 290 EVNTEILGPPTINDEKMVKFVAETAKALGLKVGEVRKTLGGEDFAYYLEKVPGAFIALGI 349 Query: 120 ----TGPAYALHHPRFDVDEAAL 138 G Y HHP+FDVDE L Sbjct: 350 RNEEKGIIYPHHHPKFDVDEDVL 372 >mlr3583 [R] COG1473 Metal-dependent amidase/aminoacylase/carboxypeptidase Length = 387 Score = 92.8 bits (229), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 57/157 (36%), Positives = 81/157 (51%), Gaps = 5/157 (3%) Query: 1 METVVLSVTRINGGHTWNVLPERVELEGTVRTYNEEIRASMPEKITRXXXXXXXXXXXXX 60 +E VV+SVT+ + G +N++PE E+ GTVRT +EI E I Sbjct: 230 VEAVVVSVTKFHAGDAYNIIPESAEIAGTVRTLRKEIAKKSEECIRTICDGLATAFGAKI 289 Query: 61 RLHWAPGPPATIN---DGVWA-DFSKEIAGQAGYEVHDIPPQMGGEDFALYLQQIPGAFV 116 + + P T N + V+A D + +AG A Y I P MGGEDF+ L+ PGAF+ Sbjct: 290 EVDYQANYPVTFNHAEETVFASDIAANVAGDA-YVHRGIQPVMGGEDFSYMLEARPGAFI 348 Query: 117 NIGTGPAYALHHPRFDVDEAALLPAARYFALLAEQAL 153 IG G LH+P +D ++ A+ Y+ LAE AL Sbjct: 349 FIGNGDTAGLHNPAYDFNDEAIPHGMSYWVKLAETAL 385 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,784,678 Number of extensions: 473195 Number of successful extensions: 766 Number of sequences better than 1.0e-05: 52 Number of HSP's gapped: 696 Number of HSP's successfully gapped: 52 Length of query: 160 Length of database: 38,378,431 Length adjustment: 98 Effective length of query: 62 Effective length of database: 27,075,699 Effective search space: 1678693338 Effective search space used: 1678693338 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 111 (47.4 bits)