BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0534.0009.p (586 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value DR2404 [V] COG1132 ABC-type multidrug transport system, ATPase a... 217 5e-56 CAC0661 [V] COG1132 ABC-type multidrug transport system, ATPase ... 210 4e-54 RSc2200 [V] COG1132 ABC-type multidrug transport system, ATPase ... 179 2e-44 XF1081 [V] COG1132 ABC-type multidrug transport system, ATPase a... 176 9e-44 YPO1395 [V] COG1132 ABC-type multidrug transport system, ATPase ... 173 8e-43 >DR2404 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 633 Score = 217 bits (552), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 140/498 (28%), Positives = 259/498 (52%), Gaps = 23/498 (4%) Query: 103 RQVNKKLSVISMEQLENPAVYDLLDRVQSNVNKGILSYINNSLSVVLPVXXXXXXXXXXX 162 R +NK + ++ LE+P +D ++ +Q + L+ ++ L ++ V Sbjct: 139 RLMNKMGELPGLDVLEDPRFHDDIEILQMGAPRRPLNLVSTLLYLIRSVISAVSVSATLL 198 Query: 163 XXXXXXXXXAVLATVPYLLLMNAQGKKSYFQAVEQSKPLRRLNYMYDVLTSRRYAKEIRM 222 V +P LL K + +++++ R +NY+ V YAKE+R+ Sbjct: 199 SIGWWVPLVVVAGMLPALLKQMEFYKLGWSIFIQRTQDSREVNYLQRVAMRHEYAKEVRL 258 Query: 223 FGLIDYFNDRTEEIRKDVWKEKFKLLLHYTLGGAFIDIFRNVALGICLLMTCIGVLENRM 282 +GL+ Y + R +++ + + + L G I AL + + + NR Sbjct: 259 YGLLPYLQSQYLS-RTLTYQQTMRGVRNKQLLG----ILPYQALSLLVTAGLFVYVVNRA 313 Query: 283 -----SIGDVMLVITSMQAITDGLSGMVNKISSMNNYTLYIEDMMNFLEL-PEDT-GSGH 335 + G V+LVIT++ + D L + +S+ + + FL+ P T Sbjct: 314 QHGTYTAGSVVLVITALAGLRDELRSISEYLSTGTEHLNWFAKFHRFLDATPGVTLAPDP 373 Query: 336 KKIVESPTIQFKHVDFKYPNSKTHTLKNIDITINKGEKIALVGENGCGKTTFINVLLGLY 395 + + ++ + V F Y ++ ++++ +TI +G+ +A+VGENG GKTT + +LL Y Sbjct: 374 RPLPRELSLTLQDVSFGY-RGQSPVIEHLSLTIPEGQTVAIVGENGAGKTTLVKLLLRFY 432 Query: 396 KPTSGEVLVGG----DPLADV-IDDFRTMTVCVFQNFIKYQLSVADNIKAGNFGEDFHK- 449 P+ G +L+GG L D+ ++ +R VFQ+F +++ ++ DN+ G ED K Sbjct: 433 DPSEGRILIGGAGEQTDLRDLDVNAWRRQVAAVFQDFARFEWTLRDNVTLGQ-PEDAAKL 491 Query: 450 -QSLQIFSMDSFIDNLPKGLDTQLGQLENNDVELSGGQWQRLAIARALSRAGTEILIMDE 508 +++ + + ++++ GLD ++GQ V+LSGGQWQ+LA ARAL R +LI+DE Sbjct: 492 SHAVEASGLGAALNHVDGGLDARIGQ-AFGGVDLSGGQWQKLATARALYRE-ARVLILDE 549 Query: 509 PTASLDPKVETQIYEEFSALCENKTAIMISHRLGVTRLCDTIYVFDQGEICEFGSHAELM 568 PTA+LDP+ E ++++ F+AL + +T ++I+HRLG + D + V +G + E G+H EL+ Sbjct: 550 PTAALDPRSEKEVFDAFAALSQGRTTLLITHRLGSVLMADRVLVMKRGRLIEDGTHTELL 609 Query: 569 NRRGKYYQMYSAQSYLYA 586 R G+Y +++ Q+ YA Sbjct: 610 TRGGEYAELWELQASQYA 627 >CAC0661 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 627 Score = 210 bits (535), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 143/490 (29%), Positives = 245/490 (50%), Gaps = 13/490 (2%) Query: 107 KKLSVISMEQLENPAVYDLLDRVQSNVNKGILSYINNSLSVVLPVXXXXXXXXXXXXXXX 166 KK + + + EN Y+ +++ S + +N +V Sbjct: 121 KKANELDLSYFENDEFYNKIEKANSQAVGTTIQIVNGLTQIVKNFTTLVGAIVIVFQLSP 180 Query: 167 XXXXXAVLATVPYLLLMNAQGKKSYFQAVEQSKPLRRLNYMYDVLTSRRYAKEIRMFGLI 226 ++ ++P ++ K+ Y ++ + R +Y+ V+ KEI++ + Sbjct: 181 IILLLCIMTSIPMFIINIKIYKQKYNIYSKRIEKTRLAHYLQMVMMDYIPVKEIKLNRIG 240 Query: 227 DYFNDRTEEIRKDVWKEKFKLLLHYTLGGAFIDIFRNV-ALGICLLMTCIGVLENRMSIG 285 D+ I KD + K+ L + +DIF + + G + + I L R+SIG Sbjct: 241 DWLEKIILSIYKDNLNQDKKIEKKQLLIMSIVDIFNTIISYGYKIYVIFI-TLTQRLSIG 299 Query: 286 DVMLVITSMQAITDGLSGMVNKISSMNNYTLYIEDMMNFLELPE---DTGSGHKKIVES- 341 + + I+++ + D + ++ + + + LYIE++ L+L + SG K+ + Sbjct: 300 SMNMYISALTNLEDSVKNTLDNFALLYSNNLYIENLFYVLDLKPLILNNNSGKKEFINEI 359 Query: 342 -PTIQFKHVDFKYPNSKTHTLKNIDITINKGEKIALVGENGCGKTTFINVLLGLYKPTSG 400 +I+F++V FKYPNSK + LKN+ I + A+VG NGCGKTT I +L LY PT G Sbjct: 360 RDSIEFRNVSFKYPNSKKYALKNVSFKIEANQNCAIVGLNGCGKTTLIKLLTRLYDPTEG 419 Query: 401 EVLVGGDPLADV-IDDFRTMTVCVFQNFIKYQLSVADNIKAGNFG--EDFH--KQSLQIF 455 E+ + + + I+ VFQ+F+KY V NI GN ++F K + + Sbjct: 420 EIYIDNINIKEFNIESLYKGIGIVFQDFMKYPFDVKKNIGFGNIDNLDNFELIKTAAKKA 479 Query: 456 SMDSFIDNLPKGLDTQLGQLENNDVELSGGQWQRLAIARALSRAGTEILIMDEPTASLDP 515 + +FI L DT+L ++ +N V+LS GQWQ+LAI+RA + +LI+DEPTAS+D Sbjct: 480 NAYTFIQGLSNKFDTKLQKMWSNGVDLSLGQWQKLAISRAF-MSNACLLILDEPTASIDA 538 Query: 516 KVETQIYEEFSALCENKTAIMISHRLGVTRLCDTIYVFDQGEICEFGSHAELMNRRGKYY 575 K E +++++F L T+I+ISHR ++ D I V GEI E G+H L+ G Y Sbjct: 539 KSEYELFKDFKELMGEGTSILISHRFSTVKMADKIIVLKDGEIVEQGNHDSLIKSDGLYA 598 Query: 576 QMYSAQSYLY 585 ++Y+ Q+ Y Sbjct: 599 ELYNMQADAY 608 >RSc2200 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 592 Score = 179 bits (453), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 105/327 (32%), Positives = 175/327 (53%), Gaps = 15/327 (4%) Query: 264 VALGICLLMTCIGVLENRMSIGDVMLVITSMQAITDGLSGMVNKISSMNNYTLYIEDMMN 323 +AL + L + + N+ ++G + +M + L + + M E + Sbjct: 268 LALSVILAIAMVQAQANQTTVGGFTGFVMAMLLLISPLKHLTDVNQPMQRGLTAAEFIFG 327 Query: 324 FLELPEDTGSGHKKIVES-PTIQFKHVDFKYPNSKTHTLKNIDITINKGEKIALVGENGC 382 ++ P + G K I + ++F+HV F+Y L +ID+ + GE +ALVG +G Sbjct: 328 LIDTPIEPQDGGKHIDRARGDLRFEHVTFRYGPDGRAALDSIDLHVKAGEIVALVGPSGS 387 Query: 383 GKTTFINVLLGLYKPTSGEVLVGGDPLADV-IDDFRTMTVCVFQNFIKYQLSVADNIKAG 441 GKTT +N+L ++PTSG +++ GD LAD+ + D R V Q+ + + ++A N+ G Sbjct: 388 GKTTLVNLLPRFFEPTSGRIVLDGDALADLSLQDLRRQIAFVSQDVVLFNDTIAANVAYG 447 Query: 442 NFGEDFH-------KQSLQIFSMDSFIDNLPKGLDTQLGQLENNDVELSGGQWQRLAIAR 494 D +++L+ + +DNLP G+DT +G +N +LSGGQ QRLAIAR Sbjct: 448 --ARDASEIDMARVRRALEAAYLTDVVDNLPDGVDTNIG---DNGSKLSGGQRQRLAIAR 502 Query: 495 ALSRAGTEILIMDEPTASLDPKVETQIYEEFSALCENKTAIMISHRLGVTRLCDTIYVFD 554 A+ + ILI+DE T++LD + E Q+ AL + +T ++I+HRL D I V + Sbjct: 503 AVYK-DAPILILDEATSALDSESERQVQAALEALMQGRTTLVIAHRLSTIENADRIVVLE 561 Query: 555 QGEICEFGSHAELMNRRGKYYQMYSAQ 581 G+I E G+H EL++R G Y ++ Q Sbjct: 562 HGQIVEAGTHRELLDRDGLYAGLHRIQ 588 >XF1081 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 589 Score = 176 bits (446), Expect = 9e-44, Method: Compositional matrix adjust. Identities = 105/336 (31%), Positives = 176/336 (52%), Gaps = 11/336 (3%) Query: 252 TLGGAFIDIFRNVALGICLLMTCIGVLENRMSIGDVMLVITSMQAITDGLSGMVNKISSM 311 +L A + + V L + LL+ L R+S GD + ++TSM A+ L + N + + Sbjct: 253 SLSSAAVQLLGAVGLAMLLLIAGHEALAGRLSPGDFVSLMTSMIAVIPALKQLTNVQNML 312 Query: 312 NNYTLYIEDMMNFLELPEDTGSGHKKIVESPT-IQFKHVDFKYPNSKTHTLKNIDITINK 370 + + + + L+ P++ +G + + + I+F+ + +YP L ++ Sbjct: 313 QSGIASAQRLFSVLDSPDELDTGRRPLGRARGFIEFRGITARYPGRSAPVLDSVSFVAAP 372 Query: 371 GEKIALVGENGCGKTTFINVLLGLYKPTSGEVLVGGDPLAD-VIDDFRTMTVCVFQNFIK 429 G A+VG +G GK++ I ++ Y+P SG++L+ G PL D ++ D R V Q + Sbjct: 373 GTVTAIVGRSGSGKSSLIKLIPRFYEPESGQILLDGHPLQDYLLADLRRQIALVGQQVML 432 Query: 430 YQLSVADNIKAGNF----GEDFHKQSLQIFSMDSFIDNLPKGLDTQLGQLENNDVELSGG 485 + S+A+NI G E+ + + + D F++ LP+GL Q+G LSGG Sbjct: 433 FDGSIAENIAYGEMRQVVSEEIERVVVDANAQD-FVNQLPEGLQFQVG---VKGGRLSGG 488 Query: 486 QWQRLAIARALSRAGTEILIMDEPTASLDPKVETQIYEEFSALCENKTAIMISHRLGVTR 545 Q QRLAIARA+ + ILI+DE TA+LD + E + + L +T ++I+HRL + Sbjct: 489 QRQRLAIARAMLK-DAPILILDEATAALDNESERLVQDALQRLMPERTTLVIAHRLSTIK 547 Query: 546 LCDTIYVFDQGEICEFGSHAELMNRRGKYYQMYSAQ 581 D + V DQG I E G+H +L+ R G Y +YS Q Sbjct: 548 HADQVLVMDQGRIIESGTHVDLLARDGLYAYLYSMQ 583 >YPO1395 [V] COG1132 ABC-type multidrug transport system, ATPase and permease components Length = 582 Score = 173 bits (438), Expect = 8e-43, Method: Compositional matrix adjust. Identities = 117/388 (30%), Positives = 197/388 (50%), Gaps = 17/388 (4%) Query: 205 NYMYDVLTSR----RYAKEIRMFGLIDYFNDRTEEIRKDVWKEKFKLLLHYTLGGAFIDI 260 N M +V TS + KE+ +FG +R + + + ++ KL+ ++ I + Sbjct: 198 NTMGEVTTSAEQMLKGHKEVLIFGGQKVETERFDAVSNRMRQQGMKLVSASSISDPIIQL 257 Query: 261 FRNVALGICLLMTCIGVLENRMSIGDVMLVITSMQAITDGLSGMVNKISSMNNYTLYIED 320 + AL + L + ++ G + +V ++M A+ L + N + + Sbjct: 258 IASFALALVLYAASFPSVMETLTAGTITVVFSAMIALMRPLKSLTNVNTQFQRGMAACQT 317 Query: 321 MMNFLELPEDTGSGHKKIVESP-TIQFKHVDFKYPNSKTHTLKNIDITINKGEKIALVGE 379 + + L++ ++ G ++ + I+F+HV F YP T L +I+I + G+ +ALVG Sbjct: 318 LFSILDMEQEKDEGKLEVERAKGDIEFRHVTFYYPGKDTPALNDINIHLEAGKTVALVGR 377 Query: 380 NGCGKTTFINVLLGLYKPTSGEVLVGGDPLADV-IDDFRTMTVCVFQNFIKYQLSVADNI 438 +G GK+T N+L Y + G +L+ G L D + R V QN + +VA+NI Sbjct: 378 SGSGKSTIANLLTRFYDVSEGSILLDGHDLRDYRLGALRNQVALVSQNVHLFNDTVANNI 437 Query: 439 KAGNFGEDFHKQSLQ-----IFSMDSFIDNLPKGLDTQLGQLENNDVELSGGQWQRLAIA 493 E + + ++ ++MD FI+ + GLDT +G+ N + LSGGQ QR+AIA Sbjct: 438 AYAR-NEQYSRAEIEEAARMAYAMD-FINKMEHGLDTVIGE---NGIMLSGGQRQRIAIA 492 Query: 494 RALSRAGTEILIMDEPTASLDPKVETQIYEEFSALCENKTAIMISHRLGVTRLCDTIYVF 553 RAL R ILI+DE T++LD + E I L +N+T+++I+HRL D I V Sbjct: 493 RALLR-NCPILILDEATSALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVI 551 Query: 554 DQGEICEFGSHAELMNRRGKYYQMYSAQ 581 + G I E G HAEL+ ++G Y Q+ Q Sbjct: 552 EDGRIVERGVHAELLVQQGVYAQLNRMQ 579 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.138 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 39,346,888 Number of extensions: 1570614 Number of successful extensions: 20161 Number of sequences better than 1.0e-05: 1817 Number of HSP's gapped: 17459 Number of HSP's successfully gapped: 1950 Length of query: 586 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 474 Effective length of database: 25,461,023 Effective search space: 12068524902 Effective search space used: 12068524902 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 119 (50.4 bits)