BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0533.0014.p (287 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH3842 [T] COG4753 Response regulator containing CheY-like recei... 83 6e-16 CAC2608 [K] COG2207 AraC-type DNA-binding domain-containing prot... 80 5e-15 BH3679 [T] COG4753 Response regulator containing CheY-like recei... 79 6e-15 lin1114_2 [K] COG2207 AraC-type DNA-binding domain-containing pr... 77 4e-14 lin0157 [K] COG2207 AraC-type DNA-binding domain-containing prot... 75 1e-13 >BH3842 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 530 Score = 82.8 bits (203), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 39/97 (40%), Positives = 62/97 (63%) Query: 187 DWIEDHFRQPFSLELLAGELHLSSYYVSHLFKQYTGVSLTDYVTARRIREACVLLGSSGQ 246 D++ DHFR+P +L+ LA + LS YYVS LFK+ ++ ++YVT R+ A LL + + Sbjct: 422 DYVHDHFREPLTLDDLAKHIGLSPYYVSKLFKERFDLTFSEYVTQLRVEHAKQLLSLTQK 481 Query: 247 SVQRIAREVGGLSAPYFCKLFKKHKGMTPLDYRSALH 283 S++ I E+G YF ++FKK G +P DYR +++ Sbjct: 482 SLKEITYEIGYKDPNYFSRVFKKLTGKSPSDYRKSIN 518 >CAC2608 [K] COG2207 AraC-type DNA-binding domain-containing proteins Length = 284 Score = 79.7 bits (195), Expect = 5e-15, Method: Compositional matrix adjust. Identities = 65/260 (25%), Positives = 114/260 (43%), Gaps = 18/260 (6%) Query: 25 HAHQGLELLFIHEGQGIVEVEGRTYELQGGSLFCFQPYQLHKVEIPAPRDARDVRYVRTN 84 H H E+ + G+ ++ + Y + G+LF ++ H V + + R Sbjct: 35 HIHDCYEIYYSISGENQFLIDNKLYYINPGNLFAVNEFESHYVS-----QVNNSTHERIV 89 Query: 85 LTFEPRYLEPFLAPYPKLEAFLRFLSRGTLSSPRFAVDD---SRLMGAVESHVQAVLEPG 141 ++ P +L+ L F RG+ + +++ SR M + + + G Sbjct: 90 ISIHPDFLKSLCTSKTNLT--YCFKHRGSNFCHKVSLNKEQQSRFMYFIN---KIIGSHG 144 Query: 142 EGQEEARILFLISLLRYL-QLAVISGEELPPEGWSVKTTAHIESVMDWIEDHFRQPFSLE 200 G + I L+ +L L + +GE L P K + IE ++ +I D+ +++ Sbjct: 145 FGADMIEYASFIELMIFLTSLFINNGESLTP---IYKYSNQIEEIIRYINDNVTNDITVK 201 Query: 201 LLAGELHLSSYYVSHLFKQYTGVSLTDYVTARRIREACVLLGSSGQSVQRIAREVGGLSA 260 LA LS Y+ +FK TG ++ Y+TARRI A LL S+G S+ + + G Sbjct: 202 HLAEHFFLSESYICRIFKLSTGTTINKYITARRISMAKALL-SNGSSINDVYVKCGFNDY 260 Query: 261 PYFCKLFKKHKGMTPLDYRS 280 F K FKK G++P Y + Sbjct: 261 SNFLKSFKKAVGISPKKYST 280 >BH3679 [T] COG4753 Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain Length = 257 Score = 79.3 bits (194), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 49/160 (30%), Positives = 85/160 (53%), Gaps = 16/160 (10%) Query: 121 VDDSRLMGAVESHVQAVLEPGEGQEEARILFLISLLRYLQLAVISGEELPP-EGWSVKTT 179 V +L+ AVES +Q E R+ Q+ + E++ E + ++ Sbjct: 109 VKKEKLIEAVESALQMKKEQD---------------RFGQVEKVWDEKIDAIEKERLSSS 153 Query: 180 AHIESVMDWIEDHFRQPFSLELLAGELHLSSYYVSHLFKQYTGVSLTDYVTARRIREACV 239 I + +I+ H Q L+ +A +HL+ Y+S LFK+ T ++ ++Y+T +R++ A Sbjct: 154 DSIHEALHYIDAHLDQKIGLKDVAKSVHLNPSYLSALFKEKTNMTFSEYLTRKRLQHAKH 213 Query: 240 LLGSSGQSVQRIAREVGGLSAPYFCKLFKKHKGMTPLDYR 279 LL S+ + + IA + G +A YF K+FK+H+GMTP YR Sbjct: 214 LLVSTDRPISDIAEKCGYQTAKYFIKIFKEHEGMTPTQYR 253 >lin1114_2 [K] COG2207 AraC-type DNA-binding domain-containing proteins Length = 145 Score = 76.6 bits (187), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 35/101 (34%), Positives = 58/101 (57%) Query: 182 IESVMDWIEDHFRQPFSLELLAGELHLSSYYVSHLFKQYTGVSLTDYVTARRIREACVLL 241 I + +IE + +P +L+ +A ++LSSYY S LFK+ V+ +YV +++ A +L Sbjct: 44 IRKALKYIEKNLNRPITLDEVASHVYLSSYYFSKLFKKEMNVNFINYVNQKKMSLAKDML 103 Query: 242 GSSGQSVQRIAREVGGLSAPYFCKLFKKHKGMTPLDYRSAL 282 + S+ +AR +G YFCK+F+K +TP YR L Sbjct: 104 KNPRWSIDNVARNLGFTQTSYFCKVFRKEFDVTPKGYRETL 144 >lin0157 [K] COG2207 AraC-type DNA-binding domain-containing proteins Length = 277 Score = 75.1 bits (183), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 70/260 (26%), Positives = 119/260 (45%), Gaps = 29/260 (11%) Query: 25 HAHQGLELLFIHEGQGIVEVEGRTYELQGGSLFCFQPYQLHKVEIPAPRDARDVRYVRTN 84 H H LE+ I EG I ++EG L+ G + F P H +I P + + + Sbjct: 30 HHHDFLEISIICEGNVIYDIEGERVALEKGDMLIFNPGVNH-YDITEP----GMTNAQLH 84 Query: 85 LTFEPRYLEPF-LAPYPKLEAFLRFLSRGTLSSPRFAVDDSRLMGAVESHVQAVLEPGEG 143 + F LE + +P +AFLR D+S A+ S + ++E + Sbjct: 85 IGFRNFALEGYSRNTFPFKKAFLRKKE-----------DES----AILSISKQIIEEKDA 129 Query: 144 QEEARILFLISLLRYLQLAVI---SGEELPPEGWSVKTTAH-----IESVMDWIEDHFRQ 195 ++ L L + + L + V+ + E+L G + T + ++ ++E H + Sbjct: 130 EKPGYDLILKAFVMQLIIHVLREATPEQLENNGVKLSTDEQQKQMLVNEIIHYMEKHHTE 189 Query: 196 PFSLELLAGELHLSSYYVSHLFKQYTGVSLTDYVTARRIREACVLLGSSGQSVQRIAREV 255 SL L+ +++S Y+S +FK+ TG S +Y+ R+ A LL + +V++ A V Sbjct: 190 DVSLSTLSQTMYISPAYISKVFKEETGESPINYLIKIRLTRAEELLKNKDVTVKQAANMV 249 Query: 256 GGLSAPYFCKLFKKHKGMTP 275 G A YF KLFKK+ G P Sbjct: 250 GYNDAYYFSKLFKKYYGFPP 269 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.139 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,854,423 Number of extensions: 950193 Number of successful extensions: 2757 Number of sequences better than 1.0e-05: 798 Number of HSP's gapped: 2731 Number of HSP's successfully gapped: 798 Length of query: 287 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 182 Effective length of database: 26,268,361 Effective search space: 4780841702 Effective search space used: 4780841702 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 115 (48.9 bits)