BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0533.0010.p (343 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0520 [KG] COG1940 Transcriptional regulator/sugar kinase 117 2e-26 TM0110 [KG] COG1940 Transcriptional regulator/sugar kinase 65 2e-10 STM0110 [E] COG0066 3-isopropylmalate dehydratase small subunit 65 2e-10 VC0993 [KG] COG1940 Transcriptional regulator/sugar kinase 62 1e-09 BH2758 [KG] COG1940 Transcriptional regulator/sugar kinase 57 5e-08 >lin0520 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 334 Score = 117 bits (294), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 86/324 (26%), Positives = 161/324 (49%), Gaps = 7/324 (2%) Query: 11 VKKMNKELVRQALKTMKQGTKSMLAQATGLSVATCGNILNELIDTGELLELDMEESSGGR 70 +K++NK+ ++ L+ TK+ +A+ T LSV T ++ +L++ E+LE D ++GGR Sbjct: 7 LKELNKKNIKSILRQQGAMTKAEIAEVTRLSVVTVNKLIRDLVENEEILEQDNSVATGGR 66 Query: 71 PARLYRYNVNFSYIACIIIRAGVKTHSLTYTVANLAGEAIKEGYQETDRMEPDMIEKLVD 130 A Y+ N NF + I ++ K + +++V NL GE + ++ +++ Sbjct: 67 RAVSYKINPNFQQVLVISLQEKWKKITYSFSVYNLLGEPEFVEDMSGEDLDITALKRNTK 126 Query: 131 QLIGEFPQIRAVGIGVPGAVNQGIINVSDIKELINVPLEASIRDKHGVEVIMDNDMNLTV 190 L+ FP+I V IGVPG G + D L+NV L ++ + + V+++ D N + Sbjct: 127 DLVCAFPKISCVVIGVPGIEIGGKLRAMDFPLLLNVQLRETLEAEVNLPVLVETDTNAAI 186 Query: 191 YGFYQKQGYDEEKSVAVVTFIEGCFPGAGMMVDGHILRGSTRFAGEIAFLPFGMSHEEQF 250 G Y+ + EE V + + E PGAG++++G IL+G AGEI +P + + Sbjct: 187 LG-YKNRPVKEENIVGLY-YPERFPPGAGLLMNGEILKGQNGLAGEIKHMPLQVDWDNFD 244 Query: 251 RQLHERTTFYSLAGRAVSSLIAIMNPETIALTGSLV--EPADVELIRQECLKYIPEMHMP 308 + E + + V ++ +PETI L + + E + +E + P +P Sbjct: 245 FSVDE---IKAHIRKMVLLTMSFYDPETIVLYTNFYFGQKDFTEELTEELKQVYPYATLP 301 Query: 309 QLMLLENPHENYMYGLITMTLESL 332 +++L +Y GL+ ++ L Sbjct: 302 KIVLSRKFTTDYRIGLLAFGIDYL 325 >TM0110 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 378 Score = 64.7 bits (156), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 66/256 (25%), Positives = 126/256 (49%), Gaps = 21/256 (8%) Query: 7 NTMRVKKMNKELVRQALKTMKQGTKSMLAQATGLSVATCGNILNELIDTGELLEL-DMEE 65 N+ R+K +NK+ + + + ++S +++ TGL+ ++ + ELID G + E+ M + Sbjct: 4 NSPRIKILNKKSILKVIHENHPISRSDISEVTGLTPSSVTRLTKELIDEGYIREIGTMGK 63 Query: 66 SSGGRPARLYRYNVNFSYIACIIIRAGVKTHSLTYTVANLAGEAIKEGYQETDRMEP--- 122 +S GR L N ++++ + + + TY + GE G T + EP Sbjct: 64 NSPGRRRILLDLRKN-AFLSLVF---DIGVNITTYGIGFFDGEVEPRGTFNTPK-EPVEF 118 Query: 123 -DMIEKLVDQLIGEFPQIRAVGIGVPGAVN--QGIINVSDIKELINVPLEASIRDKHGVE 179 ++++++ +++ GE+ +I + + VPG V+ + I ++ E NV ++ + K V Sbjct: 119 FNIVKEIYERISGEY-RISRISLSVPGMVDMEEKKILLAPNLEWENVNIKELL--KVDVP 175 Query: 180 VIMDNDMNLTVYG-FYQKQGYDEEKSVAVVTFIEGCFPGAGMMVDGHILRGSTRFAGEIA 238 V+ DN+ NL++ Y + K + EG G G+M+DG I RG + AGE Sbjct: 176 VLADNEANLSMLAEKYHSEDLRNVKEAVFIIIREGV--GTGLMIDGKIFRGPSFTAGEAG 233 Query: 239 FLPFGMSHEEQFRQLH 254 + M + RQ H Sbjct: 234 HMTVNMYSD---RQCH 246 >STM0110 [E] COG0066 3-isopropylmalate dehydratase small subunit Length = 346 Score = 64.7 bits (156), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 16/256 (6%), Positives = 51/256 (19%), Gaps = 21/256 (8%) Query: 7 NTMRVKKMNKELVRQALKTMKQGTKSMLAQATGLSVATCGNILNELIDTGELLEL-DMEE 65 + + + + T + A A G L ++ + + + + Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 66 SSGGRPARLYRYNVNFSYIACIIIRAGVKTHSLTYTVANLAGEAIKEGYQETDRMEP--- 122 + + + I + + + + + Sbjct: 64 LTETYVGNWLAKHGS-REKLIIA---SKVSGPSRNNDKGIRPDQALDRKNIREA-LHDSL 118 Query: 123 -DMIEKLVDQLIGEFPQIRAVGIGVPGAVN--QGIINVSDIKELINVPLEASIRDKHGVE 179 + +D + R + VS + L + Sbjct: 119 KRLQTDYLDLYQVHW-PQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRA--GKIRY 175 Query: 180 VIMDNDMNLTVYG-FYQKQGYDEEKSVAVVTFIEGCFPGAGMMVDGHILRGSTRFAGEIA 238 + + N+ V + +D + V + V + Sbjct: 176 IGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLL--NRSFEVGLAEVSQYEGVELLAY 233 Query: 239 FLPFGMSHEEQFRQLH 254 + + Sbjct: 234 SCLGFGTLT---GKYL 246 >VC0993 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 404 Score = 62.0 bits (149), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 64/252 (25%), Positives = 117/252 (46%), Gaps = 23/252 (9%) Query: 7 NTMRVKKMNKELVRQALKTMKQGTKSMLAQATGLSVATCGNILNELIDTGELLELDMEES 66 N VK++N V + + ++ +A + L+ A+ I +L++ G + E+ + S Sbjct: 8 NVDLVKQLNSAAVYRLIDQQGPISRIQVADVSQLAPASVTKITRQLLERGLIKEVAQQAS 67 Query: 67 SGGRPARLYRYNVN-FSYIACIIIRAGVKTHSLTYTVANLAGEAIKEGYQETDRMEPDM- 124 +GGR A V F IA I R ++ ++ +L G ++ + + E D+ Sbjct: 68 TGGRRAISLTTEVKPFHSIAVRIGRDYIQ-----LSLYDLGGNSLVDEHHEFHYNTQDVL 122 Query: 125 -------IEKLVDQLIGEFPQIRAVGIGVPGAVN--QGIINVSDIKELINVPLEASIRDK 175 I++ + Q Q+ A+G+ +PG VN G++ + +PL A+IRD+ Sbjct: 123 MSSLIKQIKQFIQQHTALIDQLIAIGVALPGLVNPETGVVEYMPNVAINELPLGATIRDE 182 Query: 176 HGVEVIMDNDM-NLTVYGFYQKQGYDEEKSVAVVTFIEGCFPGAGMMVDGHILRGSTRFA 234 VE + ND+ + + Y D + S+ +V+ G GAG++V+G + G R Sbjct: 183 FHVECFVGNDVRGIALAEHYFGASQDCQDSI-LVSVHRGT--GAGIIVNGQVFLGYNRNV 239 Query: 235 GEIAFL---PFG 243 GEI + P G Sbjct: 240 GEIGHIQIDPLG 251 >BH2758 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 386 Score = 56.6 bits (135), Expect = 5e-08, Method: Compositional matrix adjust. Identities = 49/196 (25%), Positives = 96/196 (48%), Gaps = 9/196 (4%) Query: 4 ITNNTMRVKKMNKELVRQALKTMKQGTKSMLAQATGLSVATCGNILNELIDTGELLELDM 63 +T + +K+MNK++V Q ++ +++ ++ TGL+ T +++ ELI + E+ Sbjct: 3 VTGDQRFIKQMNKQIVLQTIRKEAPLSRAQVSLTTGLNKGTVSSLVAELIADNLVYEVGP 62 Query: 64 EESSGGRPARLYRYNVNFSYIACIIIRAGVKTHSLTYTVANLAGEAIKEGY--QETDRME 121 ESSGGR + + +Y I I + LT V N+ E ++ +E D + Sbjct: 63 GESSGGRRPVMLMFQNKAAYAIGINIAVNYLSGVLTDLVGNVI-EKVEWSLHVKEFDHVN 121 Query: 122 PDMIEKLVDQLIGEFPQ----IRAVGIGVPGAVN-QGIINVSDIKELINVPLEASIRDKH 176 M++ L++QL+ P I +G+ VPG VN +G + + +PL+ + + Sbjct: 122 QQMVQ-LIEQLMKAAPPSTYGIIGIGVAVPGIVNEEGDVLFAPNLNWREIPLKKQLEEHF 180 Query: 177 GVEVIMDNDMNLTVYG 192 + + ++N+ N G Sbjct: 181 QLPIAVENEANAGALG 196 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,903,803 Number of extensions: 1109601 Number of successful extensions: 2159 Number of sequences better than 1.0e-05: 25 Number of HSP's gapped: 2170 Number of HSP's successfully gapped: 25 Length of query: 343 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 236 Effective length of database: 26,037,693 Effective search space: 6144895548 Effective search space used: 6144895548 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)